FLAVOUR AND FRAGRANCE JOURNAL
·
2023
·
IF 2.6
Yanru Chen,Yin Wan,Wenqin Cai,Xiaming Che,Hong Peng,Guiming Fu
Higher alcohols (phenylethyl alcohol and isoamyl alcohol), as the crucial aroma compounds, have been found to associate with the sensory properties of various alcoholic products. The Ehrlich and Harris pathways are the main pathways for yeast to produce phenylethyl alcohol and isoamyl alcohol during the brewing process of alcoholic products, whereas the specific changes of the Ehrlich and Harris pathways in Wickerhamomyces anomalus under ethanol stress are still unclear. In this study, W. anomalus NCU003 with a high capacity for aroma compound production was treated with ethanol stress at different concentrations (3%, v/v, 6%, v/v and 9%, v/v) to study the effects on the expression levels of genes and metabolite content in the Ehrlich and Harris pathways. The results found that ethanol stress inhibited the production of phenylethyl alcohol and isoamyl alcohol, and the content decreased with the increase of ethanol concentrations. In the meantime, the up-regulation of ARO1 , CS , ARO8 , Leu1 , BAT2 and ilvs family genes in the Ehrlich and Harris pathways could lead to an increase in the synthesis of important precursor substances during the synthesis of phenylethyl alcohol and isoamyl alcohol. However, the significant down-regulation of ARO10 , PDC , ADH1 and AHD2 genes was the main reason for the low production of phenylethyl alcohol and isoamyl alcohol. These results could provide a theoretical reference for the construction of genetic engineering strains of W. anomalus in the future so as to reasonably control the content of higher alcohols during the brewing process of alcoholic products.
研究领域:
微生物学
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生物化学
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发酵科学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Si-Tong Zheng,Zi-Ying Wang,Zhen Liu,Yingxiang Du,Ling Cao,Sheng Tang,Hian Kee Lee,Yaozuo Yuan,Hai-Wei Shi
Ethylene glycol (EG) and diethylene glycol (DEG) are two contaminants known to cause a variety of human health problems, when ingested in certain amounts, they can cause adverse effects such as nephrotoxicity, neurotoxicity and even death. They are widely found in propylene glycol, which is commonly used as a base for pharmaceutical syrups. The aim of this study was to develop an analytical method for the evaluation of EG and DEG in commercially available pediatric syrups. In this study, a fast, simple and reliable ultrahigh performance supercritical fluid chromatography-electrospray ionization-mass spectrometry (UHPSFC-ESI-MS) method was developed. The work involved the evaluation of three derivatization reagents for UHPSFC-ESI-MS. Benzoyl chloride was finally selected as the optimal derivatization reagent. Compared with an approach without derivatization, the present method achieved the separation and detection of EG and DEG efficiently, sensitively and rapidly, and analysis of EG and DEG in syrup formulations was realized within 7 min. The linear determination coefficients of EG and DEG in the concentration range of 0.25–25.0 μg/mL were greater than 0.999. The limits of detection for EG and DEG were 0.02 μg/mL and 0.07 μg/mL, respectively, and the limits of quantification were 0.09 μg/mL and 0.25 μg/mL, respectively. The recovery rates of DEG and EG ranged from 85.5%–108.1% and 86.7%–117.2%, respectively. The absolute values of the matrix effects in the three types of syrups considered were all less than 10%. Meanwhile, a gas chromatography-hydrogen flame ionization detection method was established for cross-testing of the analytical results. In 10 batches of syrup formulations, DEG was not detected by either method. The presence of EG was detected by UHPSFC-ESI-MS in only three batches, none of which exceeded 90.23 parts per million (ppm), with a mean absolute error of 5.95 ppm between the two sets of results. The developed UHPSFC-ESI-MS method was rapid, simple, efficient, sensitive and accurate for the determination of EG and DEG in genuine syrup formulations.
研究领域:
分析化学
·
药学
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产品关联
DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
·
2025
·
IF 5.8
Chunyang Han,Zhen Dong,Yahui Zhang,Haian Xia
Ethylene glycol (EG) and 1,2-propylene glycol (1,2-PG) are very important chemicals that are produced mainly from fossil resources at present. Catalytic hydrogenolysis of biomass (such as cellulose and sugar alcohols) to diols has attracted much attention in recent years. However, compared to sugar alcohols, sugars such as xylose have been scarcely used for the production of EG and PG until now due to their instability and low selectivity of diols. Xylose is an abundant and readily available bio-chemicals that can be produced from the hydrolysis of hemicellulose, but its catalytic hydrogenolysis into diols with high yields remains a great challenge. Herein, bimetallic Ru-Re/CZO(Ce 0.7 Zr 0.3 O 2 ) catalysts were synthesized and used to catalyze the hydrogenolysis of xylose to EG and PG. The nature of the catalysts is thoroughly characterized by XRD, XPS, BET, TEM, etc. It is found that Ru and Re nanoparticles are uniformly distributed on CZO alloy support formed by Ce and Zr. A very high total yield (65.0 %) of EG and 1,2-PG was achieved on the 4 %Ru-1 %Re/CZO catalyst under optimal conditions. Recycling experiments demonstrate that high xylose conversion and diol yields are maintained over four successive cycles under optimal conditions, despite a slight increase in Ru nanoparticle size observed via TEM analysis. The mechanistic study shows that xylose is first isomerized into xylulose, which undergoes retro-aldol cleavage to generate the intermediates, followed by further hydrogenation to EG and 1,2-PG. This work provides a new research avenue for the efficient preparation of diols from xylose.
研究领域:
化学工程
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生物质转化
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催化科学
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产品关联
DOI
高影响因子
Systems Microbiology and Biomanufacturing
·
2023
·
IF 0
Li Xuemei,Zhang Rui,Li Jianjiong,Feng Jinhui,Wu Qiaqing,Zhu Dunming,Ma Yanhe
Cholesterol and phytosterols can be served as the substrates for Actinobacteria to produce the precursors used in the synthesis of steroidal medicines. Cytochrome P450s from CYP125 and CYP142 families initiates the metabolism of cholesterol and/or phytosterols. In this study, we demonstrate the functional redundancy and substrate preference of five cytochrome CYP125s and CYP142s by the unmarked cyp genes deletion strains of Mycobacterium neoaurum NRRL B-3805. CYP125-3 was found to be responsible for the catalytic hydroxylation of both cholesterol and the C24-branched side-chains of phytosterols, while CYP142-2 can only oxidize cholesterol and/or cholest-4-en-3-one. The strain harboring the CYP125-3 produced 6.47 g l − 1 and 5.13 g l − 1 of 4-androstene-3,17-dione (AD) when 15 g l − 1 cholesterol or 17.5 g l − 1 phytosterols used, respectively. While, the strain harboring the CYP142-2 produced 7.10 g l − 1 AD when 15 g l − 1 cholesterol used. The other three cytochrome P450s have a smaller contribution for the degradation of cholesterol and phytosterols. CYP125-3 has the hydroxylation activity on more substrates than that of CYP142-2. This study provided possible guidance to improve the efficiency of AD and other metabolites production from different kinds of sterols by the genetic manipulation.
研究领域:
微生物学
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生物化学
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分子生物学
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生物技术
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DOI
Green Energy & Environment
·
2022
·
IF 12.781
Yang Zhou,Lice Yu,Jinfa Chang,Ligang Feng,jiujun Zhang
Low carbon alcohol fuels electrolysis under ambient conditions is promising for green hydrogen generation instead of the traditional alcohol fuels steam reforming technique, and highly efficient bifunctional catalysts for membrane electrode fabrication are required to drive the electrolysis reactions. Herein, the efficient catalytic promotion effect of a novel catalyst promoter, CoTe, on Pt is demonstrated for low carbon alcohol fuels of methanol and ethanol electrolysis for hydrogen generation. Experimental and density functional theory calculation results indicate that the optimized electronic structure of Pt–CoTe/C resulting from the synergetic effect between Pt and CoTe further regulates the adsorption energies of CO and H∗ that enhances the catalytic ability for methanol and ethanol electrolysis. Moreover, the good water activation ability of CoTe and the strong electronic effect of Pt and CoTe increased the tolerance ability to the poisoning species as demonstrated by the CO-stripping technique. The high catalytic kinetics and stability, as well as the promotion effect, were also carefully discussed. Specifically, 71.9% and 75.5% of the initial peak current density was maintained after 1000 CV cycles in acid electrolyte for methanol and ethanol oxidation; and a low overpotential of 30 and 35 mV was required to drive the hydrogen evolution reaction in methanol and ethanol solution at the current density of 10 mA cm −2 . In the two-electrode system for alcohol fuels electrolysis, using the optimal Pt–CoTe/C catalyst as bi-functional catalysts, the cell potential of 0.66 V (0.67 V) was required to achieve 10 mA cm −2 for methanol (ethanol) electrolysis, much smaller than that of water electrolysis (1.76 V). The current study offers a novel platform for hydrogen generation via low carbon alcohol fuel electrolysis, and the result is helpful to the catalysis mechanism understanding of Pt assisted by the novel promoter.
研究领域:
催化化学
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能源科学
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材料科学
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产品关联
DOI
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2023
·
IF 1.6
Dongyu Gu,Chen Fang,Jing Liu,Zhongai Jiang,Gang Li,Minjing Li,Min Zhang,Yun Huang
Salicornia europaea L. is an economic plant which can grow in saline-alkali land, beach and other heavy saline soil . In this study, the inhibitory activities of S. europaea extracts against tumor cell growth including human non-small cell lung cancer cell A549 , human liver cancer cell HepG2, and brain glioma cell U251 were screened. The results showed that the ethanol extract of S. europaea had an inhibitory effect on the proliferation of A549 cells, and the inhibition of tumor cells showed time-dose relationship. Moreover, the ethanol extract of S. europaea was extracted and fractionated with different organic solvents and the activity results indicated that the petroleum ether fraction with IC 50 of 96.5 μg mL −1 possessed the best activity against A549 cells. Then seven compounds, including 30-norhederagenin ( 1 ), 24-methylenecycoartanol ( 2 ), pheophorbide A ( 3 ), stigmasterol ( 4 ), β -sitosterol ( 5 ), α-spinasterol ( 6 ), phytol ( 7 ) were isolated and identified from the petroleum ether fraction of S. europaea . Besides, the oil obtained from the petroleum ether fraction was analyzed by GC-MS, and fourteen compounds were identified. The compounds identified from the petroleum ether fraction mainly consisted of triterpenes , sterols, fatty acids, and their lipids .
研究领域:
药理学
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植物科学
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癌症研究
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生物化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Kang Zhao,Jinhu Sheng,Shikai Ma,Junhao Yin,Nairui Luo,Junwei Ding,Heng Wang,Shiwen Wang,Shaoming Fang
For the development of electrolyte additives as an effective strategy to boost the reversibility of Zn anodes, most researchers focus on the functional groups but overlook the crucial molecular structure. Herein, ethylene glycol (EG), erythritol (Eryt) and hexanehexol (Hex) featuring with different molecular length and varied amounts of hydroxyl groups were introduced into 2 M ZnSO 4 electrolyte system as additives. Based on the theoretical computations and experimental analysis, the differences of three additives in tailoring the Zn anode/electrolyte interface were studied at the molecular level. Among these additives, EG additive with short molecular length and less hydroxyl groups delivers suitable interfacial adsorption energy and slightly stronger than that of water molecule, which could stabilize the anode/electrolyte interface most effectively. Therefore, with the EG additive in ZnSO 4 electrolyte, the undesired Zn dendrite growth and parasitic reactions including hydrogen evolution reaction, corrosion and passivation are significantly limited during the Zn plating/stripping process. As a result, employing EG as the electrolyte additive could greatly extend the cycling stability of Zn/Zn, Zn/Cu and Zn/V 2 O 5 batteries. This work proposes a new insight toward the optimal molecular configuration of additive design for electrolyte engineering in high-performance aqueous Zn batteries.
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
VACUUM
·
2024
·
IF 3.8
Kaile Shi,Haodong Zheng,Yuze Sun,Honghai Wang,Jing Fang,Chunli Li,Jiapeng Liu
The utilization of 1,4-butanediol (BDO) spans across numerous sectors, underscoring its significance as a pivotal chemical. However, the separation of crude BDO still presents great challenges. Ethylene glycol (EG) and 3-methyl-1,5-pentanediol (MPD) are classical impurities in BDO crude products. In this paper, the pressure and temperature data of MPD components under high vacuum pressure were measured, and Antoine constant was obtained by fitting to supplement the basic data. The isobaric vapor-liquid equilibrium (VLE) data for the EG-BDO and BDO-MPD mixtures were experimentally measured at pressures of 1.0, 10.0, and 50.0 kPa. Meanwhile, the thermodynamic consistency of the experimental binary data was examined using the Herington area test. The experimental VLE data for binary systems were analyzed using the nonrandom two-liquid (NRTL) universal quasichemical (UNIQUAC) and Wilson activity coefficient models, demonstrating that the predicted outcomes showed excellent agreement with the measured data. Finally, the interaction energy between the components was calculated by molecular simulation to explain the gas-liquid equilibrium behavior. Therefore, this work provided guidance and basis for the separation of EG-BDO and BDO-MPD in high vacuum separation.
研究领域:
化学工程
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物理化学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2025
·
IF 4.8
Daguo Wu,Yong Liu,Xinghua Zhang,Jianguo Liu,Qi Zhang,Longlong Ma,Lungang Chen
The effective utilization of biomass is essential for addressing environmental challenges. The direct conversion of cellulose into chemicals and fuels is a key step in fostering a sustainable society. In this study, a mesoporous Pt-WO x /Nb 2 O 5 catalyst is synthesized and characterized using N 2 sorption, XRD, ICP, CO pulse, TEM, SEM, NH 3 -TPD, PY-IR, H 2 -TPR, and XPS. Under optimized catalyst preparation and reaction conditions, the yields of ethanol and propanol reach 50.3 % and 20.2 %, respectively. The mesoporous structure of Nb 2 O 5 , coupled with Pt, provides a rich balance of acidic and hydrogenation-active sites. Additionally, WO x plays a crucial role in the C C bond cleavage of cellulosic glucose and the C O bond cleavage of ethylene glycol, facilitating ethanol formation through Pt and the abundant Lewis acid sites from Nb 2 O 5 .
研究领域:
化学工程
·
可再生资源
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产品关联
DOI
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2022
·Vol. 54
·期 2
·191-200
·
IF 2.727
Yuhao Xie,Siwei Yang,Shenghua Zhou,Jiazhen Liu,Shuai Zhao,Shangzhong Jin,Qiang Chen,Pei Liang
Raman spectroscopy is widely used in the identification of substances. Raman spectra contain molecular information from various components and interference from noise and instruments. Therefore, using Raman spectroscopy to identify components is still challenging, especially for substances with high similarity. In this study, a class of highly similar products (methanol and ethanol) and a multicomponent mixture of propanol and water were tested using a portable Raman spectrometer. They are divided into 11 categories according to the volume fraction ratio. A total of 5,060 groups of Raman spectrum data were obtained, constituting the data set of this study. The deep neural network structure adopted in this study is based on ResNet architecture, on which the SE module in the attention mechanism is added, which increases the weight of some spectral features and achieves significant performance improvement. Finally, SE-ResNet achieves a recall of 0.95, a precision rate of 0.95, a Micro-F1 of 0.95, and an accuracy rate of 99.20%.
研究领域:
分析化学
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人工智能
·
光谱学
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产品关联
DOI
高影响因子
Food & Function
·
2025
·
IF 5.4
Kuang Yao,Feng Yao,Xue Songwen,Yun Huang,Qiong Shao,Lichun Chen
Sugar alcohols are a class of low digestible carbohydrates that are commonly used as sweeteners and humectants in foods. Sugar alcohols of different chain lengths may have different effects on the structure and function of cell membranes, but their specific mechanisms of action and their relationship with osmosis have not been systematically studied. In this study, the effects of three sugar alcohols with different chain lengths, erythritol (Ery), xylitol (Xyl) and sorbitol (Sor), on the permeability of cell membranes were investigated using living cell membranes and artificial multilamellar vesicles (MLVs) models. The results of fluorescence microscopy showed that Ery had the greatest effect on the structure of MLVs, resulting in significant deformation of MLVs. This was closely followed by Xyl and Sor, which had relatively little effect on vesicle structure. In addition, cellular experiments showed that Ery failed to significantly increase intracellular reactive oxygen species (ROS) levels as well as maintain the membrane potential cell membrane potential at the same concentration compared to Xyl and Sor, which enhanced membrane adsorption and slowed down the decrease of membrane potential. These results suggest that sugar alcohols with different chain lengths have different permeation effects on biological membranes and provide a theoretical basis for understanding their functions in physiological environments.
研究领域:
食品科学
·
生物物理学
·
膜生物学
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碳水化合物化学
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细胞生理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Dan Du,Zhirong Qu,Weiwei Zhang,Hong Dong,Chuan Wu
The density, viscosity and speed of sound of trimethoxylsilane derivatives with ethanol, 1-butanol, 1-pentanol and 1-octanol have been measured at 298.15, 303.15, 308.15, 313.15 K, 318.15 and 323.15 K, respectively. The excess molar volume, viscosity deviation, excess Gibbs energy of activation of viscous flow, isobaric thermal expansivity and isentropic compressibility deviation were calculated from these results. The Redlich–Kister equation was used for the correlation. The calculated excess properties have been discussed according to the possible interactions formed between the molecules in the mixture using equimolar attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. In all of the compositions and experimental temperature ranges studied, the excess molar volumes were found to be negative for vinyl trimethoxy silane and phenyl trimethoxy silane with ethanol. This may be attributed to the close accumulation of molecules and easier formation of cross-association structures between the alkoxysilane molecules. However, the excess molar volume of the binary mixture of vinyl trimethoxy silane and phenyl trimethoxy silane with n -butanol, n -pentanol, and n -octanol was positive. Hydrogen bond fracture may play a leading role in this process. The excess molar volumes of the binary system comprised of trifluoropropyl trimethoxy silane with ethanol and 1-octanol were all positive. This may be attributed to the influence of steric hindrance, which makes it difficult for trifluoropropyl trimethoxy silane to form a cross-association structure with alcohol molecules.
研究领域:
物理化学
·
材料科学
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DOI
高影响因子
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