CNS Neuroscience & Therapeutics
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2023
·Vol. 29
·期 4
·1000-1011
·
IF 5.5
Di Zhang,Bei Jing,Zhen-ni Chen,Xin Li,Hui-mei Shi,Ya-chun Zheng,Shi-quan Chang,Li Gao,Guo-ping Zhao
Introduction Sciatica causes intense pain. No satisfactory therapeutic drugs exist to treat sciatica. This study aimed to probe the potential mechanism of ferulic acid in sciatica treatment. Methods Thirty-two SD rats were randomly divided into 4 groups: sham operation, chronic constriction injury (CCI), mecobalamin, and ferulic acid. We conducted RNA sequencing, behavioral tests, ELISA, PCR, western blotting, and immunofluorescence analysis. TAK-242 and JSH23 were administered to RSC96 and GMI-R1 cells to explore whether ferulic acid can inhibit apoptosis and alleviate inflammation. Results RNA sequencing showed that TLR4/NF-κB pathway is involved in the mechanism of sciatica. CCI induced cold and mechanical hyperalgesia; destroyed the sciatic nerve structure; increased IL-1β, IL-6, TNF-α, IL-8, and TGF-β protein levels and IL-1β, IL-6, TNF-α, TGF-β, TLR4, and IBA-1 mRNA levels; and decreased IL-10 and INF-γ protein levels and IL-4 mRNA levels. Immunohistochemistry showed that IBA-1, CD32, IL-1β, iNOS, nNOS, COX2, and TLR4 expression was increased while S100β and Arg-1 decreased. CCI increased TLR4, IBA-1, IL-1β, iNOS, Myd88, p-NF-κB, and p-p38MAPK protein levels. Treatment with mecobalamin and ferulic acid reversed these trends. Lipopolysaccharide (LPS) induced RSC96 cell apoptosis by reducing Bcl-2 and Bcl-xl protein and mRNA levels and increasing Bax and Bad mRNA and IL-1β, TLR4, Myd88, p-NF-κB, and p-p38MAPK protein levels, while ferulic acid inhibited cell apoptosis by decreasing IL-1β, TLR4, Myd88, p-NF-κB, and p-p38MAPK levels and increasing Bcl-2 and Bcl-xl levels. In GMI-R1 cells, Ferulic acid attenuated LPS-induced M1 polarization by decreasing the M1 polarization markers IL-1β, IL-6, iNOS, and CD32 and increasing the M2 polarization markers CD206, IL-4, IL-10 and Arg-1. After LPS treatment, IL-1β, iNOS, TLR4, Myd88, p-p38MAPK, and p-NF-κB levels were obviously increased, and Arg-1 expression was reduced, while ferulic acid reversed these changes. Conclusion Ferulic acid can promote injured sciatic nerve repair by reducing neuronal cell apoptosis and inflammatory infiltration though the TLR4/NF-κB pathway.
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JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 61
·期 7
·542-552
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IF 3.046
Jianan ShangGuan,Yanglei Zheng,Junyi Jiang,Yong Li,Haoran Sun,Shuangfei Xiang,Shujun Zhao,Feiya Fu,Xiangdong Liu
Styrene has been widely used as a reactive diluent for a variety of commercial unsaturated polyester resins (UPRs), but suffered from high volatility. In this work, we developed a bio-based reactive diluent, 4-vinylguaiacol glycidyl ether (DE4VG), via an epoxidation of 4-vinylguaiacol (4VG). Excellent miscibility and high reactivity of DE4VG were demonstrated by a series of copolymerization tests with an unsaturated polyester prepolymer (named PMSD) synthesized from maleic anhydride, 1,3-dihydroxypropane and succinic acid. While, significant advantages in terms of viscosity and volatility were confirmed. Additionally, DE4VG provides both vinyl and epoxide groups and the epoxide groups were cured using 4,4-diamino diphenylmethane (DDM) to participate the network formation of the cured PMSD/DE4VG/TBPB/DDM resin, resulting in high glass transition temperature ( T g , 195°C), residual carbon (45.5%), tensile strength (41.75 MPa), and the elongation at break (4.5%), which were all significantly surpassed the cured PMSD/St/TBPB, PMSD/St/TBPB/DDM, PMSD/DE4VG/TBPB resin. This work provided a successful demonstration to replace styrene with a bio-based reactive diluent in UPR preparation, not only overcame the high volatility of styrene, but also enhanced material performances of the cured resin. Graphical
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 5
·2247-2257
·
IF 3.3
Jiao Wu,Bingjun Han,Xiaoyu Chen,Jinglin Gao,Shan Zhao,Liping Sun,Shijie Wang
Summary Stingless bee honey is a precious food product with therapeutic benefits. In this study, we determined the quality of Malaysian stingless bee honeys ( Heterotrigona itama and Tetrigona binghami ) by the physicochemical characteristics, phenolic compounds, and organic acids. Our results revealed that the major phenolic compounds in all honey samples were chlorogenic acid and myricetin, and the major organic acids contained gluconic acid and trans -cinnamic acid. The microbial community in honeys characterised using 16S rRNA and ITS amplicon sequencing techniques showed that Lactobacillus was assumed to be the predominant bacterium in stingless bee honeys. Furthermore, the antibacterial activity of honeys was examined in order to further demonstrate their health benefits. Our results are intended to pave the road for stingless bee honeys' probiotic capacity to be extended into our daily life.
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Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 6
·2595-2617
·
IF 11.9
Ruijin Li,Danlian Huang,Lei Lei,Sha Chen,Yashi Chen,Guangfu Wang,Li Du,Wei Zhou,Jiaxi Tao,Haojie Chen
Considering the two limitations of the biocompatibility of metal–organic-frameworks (MOFs) and the low utilization of lignin biomass, herein, we review the interaction between lignin macromolecules and MOFs to promote their high-end applications and sustainability. Firstly, lignin and lignin derivatives can coordinate with metal ions to form bio-based MOFs due to their biocompatibility and surface-rich functional groups, which solves the difficulty of applying the original MOFs in biomedicine and other fields. We introduce the structure, coordination process, and advantages of applying bio-based MOFs in detail and discuss the problem that powdered MOFs are easily blocked and difficult to be separated and recovered. Secondly, MOFs can be used as catalysts to facilitate the conversion of lignin to liquid fuels and fine chemicals, thereby alleviating the energy shortage. We discuss the mechanism and products from the redox depolymerization of lignin using MOFs and their derivatives. The opportunities and challenges encountered in bio-based MOFs and selective cleavage of lignin are discussed based on several perspectives. It is hoped that this study can provide good theoretical guidance for the synthesis of bio-based MOFs and the conversion of lignin.
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Frontiers in Plant Science
·
2022
·
IF 6.627
Shi Xia,Fang Jiaxing,Du Huicong,Zhang Sufang,Liu Fu,Zhang Zhen,Kong Xiangbo
When Ips bark beetles invade and colonize the host plants, their associated pathogenic fungal partners are carried into the phloem of the host trees. Host trees are lethally attacked by the beetle-fungus complex and the collective damage severely limits forestry production worldwide. It is of great importance to verify whether bark beetles and their associated fungi show concordant performance in terms of biology, physiology, and biochemistry on host trees. In this study, the two Ips bark beetles Ips typographus and Ips subelongatus (Coleoptera: Curculionidae, Scolytinae), their respective associated pathogenic fungi Endoconidiophora polonica and Endoconidiophora fujiensis, and their respective host plants Picea jezoensis and Larix olgensis were selected as test material. Cross-inoculation experiments were conducted indoors and outdoors to investigate the differences in reproduction and development of two beetles and infectivity of two fungi on two plants, as well as the differences in physiological responses of two plants to two fungal infections. The results showed that I. typographus and E. polonica had excellent host performance on P. jezoensis; however, neither successfully colonized and infected L. olgensis. In contrast, I. subelongatus and E. fujiensis showed strong host suitability on L. olgensis and some degree of suitability on P. jezoensis, although the host suitability of P. jezoensis for E. polonica was significantly higher than that for E. fujiensis. In addition, we found that the absolute amount of ergosterol accumulated on the lesion was positively correlated with lesion area. The ergosterol amount and lesion area were both strongly correlated with the release of host monoterpenes, but had no obvious correlation with the concentration of fungi-induced phenols on the lesion area and the side-chain oxidation of lignin in the xylem of the infected sites. Based on these results, we confirmed that “I. typographus-E. polonica” and “I. subelongatus-E. fujiensis” complexes both showed the most suitable consistent performances on their own traditional hosts, establishing a stable species-specific association relationship in these two beetle-fungus complexes, with the “I. subelongatus-E. fujiensis” complex showing broader host suitability. From the perspective of physiological responses of plants to fungal infections, monoterpenes are an important indicator of host suitability.
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JOURNAL OF MOLECULAR STRUCTURE
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2022
·
IF 3.841
Fan-Zhi Bu,Yue-Ming Yu,Yu-Li Shen,Zhi-Yong Wu,Yan-Tuan Li
The present investigation aims to fully exploit the structural dominance and liver protective efficacy of phenolic acid nutraceutical ferulic acid (FUA) towards decreasing antifungal drug 5-fluorocytosine (FTS) in solubility and dissolution rate through a cocrystallization stratagem, thereby achieving some fire-new understandings for addressing the side effects generated by the fast and inappropriate absorption from FTS. Toward this end, the cocrystalline complex of FTS with FUA, viz . FTS-FUA, as the first cocrystal of FTS with phenolic acid nutrient of hydroxycinnamic acid subgroup is directionally assembled and fully characterized. The crystal structure determined by the single-crystal X-ray diffraction method demonstrates that FUA molecules in the lattice seize the polar sites in situ of FTS founded on the compact hydrogen-bond interaction, therefore leading to the formation of a rigid S-shaped module (RSM) with hydrophobic potentials, which induces a simultaneous down-regulation of the cocrystal in solubility and intrinsic dissolution rate versus raw drug FTS itself. The experimental outcomes are strongly verified by relevant theoretical investigations. The molecular electrostatic potential and the mutual penetration distance authenticate that the RSM's polarity in the cocrystal is lower than that of FTS per se, which complies with the abatement in polarity attributable to the reinforced hydrogen-bonds energy as per topology analysis with atoms in molecules theory, thus giving a remarkable improvement in hydrophobicity for the cocrystal. As a result, the current contribution not only provides a new crystallization form for FTS, but also exemplifies the feasibility of cocrystallization clue from integrated experimental with a systematic theoretical approach to overcome intractable adverse effects of the antifungal agent.
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Food Bioscience
·
2023
·
IF 5.2
Guanghe Zhao,Ruifen Zhang,Lihong Dong,Mei Deng,Yanxia Chen,Mingwei Zhang
Bound phenolics are the main phytochemicals and important contributors to the health benefits of whole grains . The commonly used chemical methods for bound phenolic release are not appropriate for processing of food materials. The effects of cellulase and feruloyl esterase on releasing bound phenolics from rice bran dietary fibre (RBDF) and the auxiliary effects of autoclaving pretreatment were investigated in the present study to find a method to effectively increase bioaccessible phenolics in RBDF. The results showed that cellulase (CT) and feruloyl esterase (FT) alone and combined application (CFT) released limited bound phenolics. Autoclaving pretreatment could enhance the release of bound phenolics by enzymatic treatments significantly with 1.74, 5.56 and 1.76 folds increases when compared with the above mentioned enzymolysis, respectively. Moreover, the total quantity of phenolic compounds released from RBDF via combined enzymolysis of cellulase and feruloyl esterase with autoclaving pretreatment (A-CFT) were comparable with that via alkaline hydrolysis previously reported. The predominant phenolic compounds released by A-CFT from RBDF were vanillin and ferulic acid , while ferulic and p -couramic acids accounted for 99.30% of the sum of phenolics released by alkaline hydrolysis. The DPPH scavenging, FRAP and ORAC antioxidant activities of the enzymatic hydrolysates were positive correlated with their total phenolic contents. The phenolics released by A-CFT showed lower antioxidant activity than those by alkaline hydrolysis, which may ascribe to the difference in phenolic profiles between methods. This study provides a new approach for processing whole grain and the by-products for functional foods materials with higher phenolic bioaccessibility.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Daihong Gao,Denghao Ouyang,Xuebing Zhao
Lignin was oxidized and depolymerized by a direct biomass fuel cell (DBFC) for electricity generation and production of aromatic aldehydes. The yield of aromatic aldehydes could be easily controlled by changing the anodic catalyst, cathodic oxidants and external loading. Cobalt and (VO 2 ) 2 SO 4 were screened as the most suitable anodic catalyst and cathodic oxidant, respectively. A maximum power density of 199.8 mW/cm 2 was obtained under the optimized conditions for electricity generation; however, the yield of the aromatic aldehydes was greatly dependent on the type of lignin used, reaction conditions and the external loading (output voltage) of the DBFC. At a lignin concentration of 0.5 g/L, aromatic aldehydes yield of 7.8 % was obtained at an output voltage of 0.9 V. The formation of aromatic aldehydes was mainly caused by cleavage of β-O-4 linkage and oxidation of side chains by the hydroxyl radicals formed via oxidation of OH – by Co 3+ on the anode.
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JOURNAL OF FOOD AND DRUG ANALYSIS
·
2023
·
IF 3.6
Jingyuan Zeng,Xican Li,Rongxin Cai,Chunhou Li,Shaoman Chen
The study combined UHPLC-Q-Orbitrap-MS analysis with authentic standards, to create a novel strategy for isomers recognition and putative identification. Through the strategy, anti -Covid-19 Jinhua Qinggan Granule was found to comprise 28 isomers and 45 potential anti -Covid-19 constituents. The detection of three constituents (Danshensu, cryptotanshin, and tanshinone IIA) suggests Danshen as confidential additive. Based on this, 6 constituents are recommended as quality-marker candidates, including chlorogenic acid, acteoside, peimisine, baicalein, licoricesaponin H2, and tanshinone IIA. Obviously, the study can not only help the public to really understand the Granule’s formula and chemistry, but also facilitate its Pharmacopoeia collection in future.
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ChemistrySelect
·
2023
·Vol. 8
·期 23
·e202203483
·
IF 2.1
Wenyao Peng,Shanshan Wang,Junjian An,Chenyang Hu,Jian Zhang,Peng Wang
Graphical GO-Cu 2 (OH) 3 NO 3 composite is prepared by using thermal calcination method. The composite exhibits larger specific surface area and stronger heterogeneous catalysis activity than those of Cu 2 (OH) 3 NO 3 . Under optimal conditions, ferulic acid could be degraded efficiently by heterogeneous process catalyzed by the composite. The environmental-friendly composite exhibits stable chemical properties, which could be reused and recycled successfully. Graphene oxide (GO)-Cu 2 (OH) 3 NO 3 composite was prepared by a thermal calcination process and evaluated as an effective heterogeneous catalytic material. The composite displayed prominent activated performance to persulfate, which was influenced by preparation condition and the reaction parameters in catalytic system. Under optimal reactive conditions, the GO-Cu 2 (OH) 3 NO 3 composite yielded rapid degradation of ferulic acid, which the corresponding apparent rate constant was 1.12×10 −1 min −1 . Catalytic mechanism analysis showed that the main oxygen species were ⋅SO 4 − and ⋅OH. Among them, ⋅OH made the main contribution in the catalytic system. The analysis of degradation intermediates of ferulic acid showed that the compound could be mineralized to small molecules. The remarkable enhanced heterogeneous catalytic performance of GO-Cu 2 (OH) 3 NO 3 was due to a larger specific surface area, high adsorption capacity and a high mass transfer efficiency of oxidizing radicals in the reactive system.
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NEW JOURNAL OF CHEMISTRY
·
2023
·
IF 3.3
Wenbin Jin,Kaipeng Wang,Xueyang Xu,Yiling Xu,Fengwei Li,qingzhi ji,Xiaodong Chen,Xiaohong Yu
Ferulic acid is a natural phenolic chemical which widely present in plants and widely used in food and cosmetic industries. It is used as the cross-linking agent for the preparation of edible films, and as skin protection agents. Enzymatic hydrolysis of lignocellulose to produce ferulic acid has been studied for many years and is rarely used in the industry due to easy deactivation of enzymes. Deep eutectic solvent (DES) is exploited as a novel co-solvent for improving the activity and stability of enzyme. A mixed enzyme system composed of feruloyl esterase and xylanase was constructed for catalytic degradation of de-starched wheat bran (DSWB) to produce ferulic acid. When the ratio of feruloyl esterase to xylanase reached 1:1, the release of ferulic acid reached the maximum. Four DES was synthesized and evaluated the effects of compositions and concentrations on the release of ferulic acid from DSWB. The mixed enzymatic system showed the best performance in the ChCl-Gly DES aqueous solution (volume ratio of 1:2). Through the determination of the enzymatic properties and catalytic conditions, the mixed enzymatic system was applied to the catalytic reaction. Ferulic acid yield 4.2 mg/g DSWB was obtained under the optimum conditions. The results suggested that the efficient production of ferulic acid from lignocellulosic biomass can be realized through the synergistic effect of feruloyl esterase and xylanase in DES aqueous solution.
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Food & Function
·
2023
·Vol. 14
·期 14
·6707-6717
·
IF 6.1
Shuai Zhang,Mei Deng,Ruifen Zhang,Xuchao Jia,Fei Huang,Dong Zhao,Lihong Dong,Jianwei Chi,Zhida Sun,Qin Ma,Mingwei Zhang
Black rice (Oryza sativa L.) is a great source of anthocyanins and dietary fiber and possesses various health-promoting properties. The modulating effect of insoluble dietary fiber (IDF) from black rice on the fermentation of cyanidin-3-O-glucoside (Cy3G) in an in vitro human colonic model, together with the possible microbiota-mediated mechanisms, was investigated. The combined Cy3G and IDF fermentation can promote the biotransformation of Cy3G into phenolic compounds such as cyanidin and protocatechuic acid with stronger antioxidant activities and increase the total production of SCFAs during the fermentation of Cy3G. 16S rRNA sequencing analysis revealed that the addition of IDF modulated the microbiota structure and bloomed Bacteroidota and Prevotellaceae-related genera, which were positively correlated with metabolites of Cy3G, thus potentially regulating the microbial metabolism of Cy3G. The work is of great significance for elucidating the material basis of the health benefits of black rice.
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危险品化学品经营许可证（带存储）