Journal of Pharmaceutical Analysis
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2013
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IF 0
Zheng-Jun Cheng,Hong-Mei Zhao,Qian-Yong Xu,Rong Liu
The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins within site I. The thermodynamic parameters indicated that the binding of indigotin–HSA/BSA mainly depended on the hydrophobic interaction. The binding distance of indigotin to HSA/BSA was evaluated. The results by synchronous fluorescence, three-dimensional fluorescence, Fourier Transform Infrared spectroscopy (FT-IR) and circular dichroism (CD) spectra showed that the conformation of proteins altered in the presence of indigotin.
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PubMed
Analytical Methods
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2013
·Vol. 5
·期 4
·925-930
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IF 1.855
Yajing Lei,Shujie Zhang,Lizheng Fang,Muhammad Sajid Hamid Akash,Weixing Shi,Kedan Sun,Yingchun Xu,Shuqing Chen
Tartrazine is commonly used in foodstuffs, its level is controlled by government, however, it is still used in some food products at levels which are not permitted. We aimed to develop a sensitive and simple method to assess the exposure to tartrazine in pregnant women and the general population in China. A competitive enzyme-linked immunosorbent assay (ELISA) was developed based on polyclonal antibodies for tartrazine. Using this assay, we measured the urinary concentrations of tartrazine in the participants. Under optimal experimental conditions, the standard curve was constructed at concentrations of 0.04–1000 ng mL−1 and the limit of detection (LOD) was set at 0.04 ng mL−1. No cross-reactivity of the antibody was observed with seven other artificial dyes based on this assay. Human blank urine was spiked with tartrazine at concentrations of 0.1, 25, 500 ng mL−1 and analyzed by ELISA, the recoveries of tartrazine were from 99.8% to 106.6% and coefficients of variation were from 3.28% to 14.9%. The study comprised of 351 volunteers (age: 19–69 years), of which 30% were male, 16% were nonpregnant women and 54% were pregnant women. The geometric means for tartrazine in three groups were 2.64, 2.44 and 3.21, respectively. No difference was observed in these groups. The values indicated that both the general population and pregnant women are exposed to tartrazine. Sources of exposure, potential health effects and risk assessment require further investigation.
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WATER RESEARCH
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2014
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IF 5.323
Ruijuan Qu,Bingzhe Xu,Lingjun Meng,Liansheng Wang,Zunyao Wang
As a promising disinfection technique to replace chlorination, ozonation has been demonstrated to be efficient in water treatment . This paper describes an effective way to enhance the ozonation of indigo by using carbon nanotubes functionalized with carboxyl groups (CNTs-COOH) as catalysts. The result of kinetic studies showed that the presence of CNTs-COOH dramatically increased the decolorization rate of indigo. Different types of catalysts were compared to further elucidate the internal mechanism of the catalytic reaction and the special nanostructure and the functional –COOH groups are considered to play an important role in the catalytic ozonation process. Four aromatic intermediate products were identified using an electrospray time-of-flight mass spectrometer and further rationalized by the frontier electron density calculations. Ion chromatography analysis revealed that the nitrogen atom of indigo was released predominantly as ammonium and to a lesser extent as nitrate. The presence of the catalyst CNTs-COOH leads to a higher mineralization degree than single ozonation, as suggested by the total organic carbon (TOC) measurement. Three major carboxylic acids (i.e., oxalic, formic and acetic acids) were also identified as oxidation by-products, and they contributed significantly to the residual TOC after 2 h of ozonation. In addition, the toxicity evolution during the degradation was investigated through two aquatic model species to evaluate the potential ecological risks of the intermediate products.
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2015
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IF 2.999
Hong Xie,Xiaoliang Ye,Kaiyue Duan,Muyin Xue,Yongling Du,Weichun Ye,Chunming Wang
The bimetallic alloy CuAu nanoparticles (NPs) can produce more photogenerated electrons when compared with single metal Au NPs. Moreover, graphene (Gr) sheets can help the charge separation and slow down the recombination of the electron hole pairs of ZnO. Hence, a novel graphene-based bimetallic alloy-semiconductor catalyst: CuAu–ZnO–Gr nanocomposite is synthesized. Due to the synergistic effect among CuAu NPs, ZnO nanopyramids, and Gr sheets, CuAu–ZnO–Gr behaves an enhanced photocatalytic activity for the photocatalytic degradation of synthetic colorants methyl orange (MO), methylene blue (MB), indigotin (IN), sunset yellow (SY), and tartrazine (TT) under the simulated sunlight irradiation. Furthermore, the apparent rate constants ( k app ) of MO, MB, IN, SY, and TT degradation are estimated respectively. In addition, the as-prepared CuAu–ZnO–Gr nanocomposite is characterized by X-ray diffraction, UV–vis spectrum, transmission electron microscopy, energy dispersive X-ray analysis (EDX), and EDX mapping. As a result of the facile synthesis route and the enhanced photocatalytic activity, this new material CuAu–ZnO–Gr can be a promising photocatalyst for the degradation of dyes.
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DESALINATION
·
2024
·
IF 8.3
Ruixue Meng,Xinxin Wang,Deping Li,Kewei Zhang,Xiankai Li,Yanhui Li,Long Chen,Lijie Ci
Effective organic dyes selective separation from high salinity textile wastewater consisting of inorganic salts and organic dyes is of great significant for the textile wastewater treatment . Graphene oxide (GO) have been used widely to form ultrathin high-permeance membrane for molecular and ionic sieving. However, swelling and membrane fouling of GO membrane hampered its practical applications. In this work, high stability and antifouling reduced graphene oxide (rGO) membranes were prepared, and Fe 2 O 3 nanoparticles was used as intercalation to turn the membrane interlayer spacing. The rGO/2Fe 2 O 3 nanocomposite blended membrane exhibited high permeance of 24.90 L m −2 h −1 bar −1 , excellent organic dyes rejection (98.15 % and 98.69 % for methylene blue and Evans blue, respectively) and low salt retention (6.26 % for NaCl). Meanwhile, this membrane demonstrated a superior selective dye/salt separation factor up to 232 with a high rejection of 99.59 % to Evans blue and a lower salt rejection of 5.03 % to NaCl. Furthermore, nanofiltration experiments towards Evans blue/NaCl mixed solution indicated that the membrane also exhibited excellent antifouling performance with flux recovery rate of >86 % after six fouling/backwash cycles. Therefore, our work may provide a promising idea for the development of two-dimensional nanofiltration membranes for efficient high salinity textile wastewater treatment.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Jingjiu Mu,Lei Wang,Fan Wang,Weihong Liao,Hailing Dai,Yajing Yang,Yanjun Jiang,Lijuan Liu,Xiao Men,Haibo Zhang
Bioconversion can efficiently funnel the complex component of biomass into desired products, and improving the utilization efficiency of the bulk biomass has significant implications. Many mature processes have been established through the combination of pretreatment strategies and adapted strains, which focus solely on the lignocellulosic part of biomass. However, few processes have been designed for bioconversion of the full biomass component. In this study, a whole process design strategy was validated via converting full corn stover into single cell protein (SCP) and blue pigment indigoidine, respectively. The corresponding reduction in CO 2 emissions was assessed using life cycle assessment (LCA). By optimizing alkali treatment conditions, the depolymerization of full corn stover was streamlined from a traditional 12-step process to a more efficient 4-step One-Pot Process, incorporating in-situ lignin depolymerization. The optimized One-Pot Process using 0.1 g/g NaOH at 100 °C depolymerized full components of corn stover, and provided a balanced nutrition profile. The selected robust strain Rhodosporidium toruloides converted the components of depolymerized full corn stover to higher SCP and indigoidine compared to equivalent glucose, with the SCP and indigoidine yield reaching 208 g/kg and 72 g/kg, respectively. Furthermore, the LCA results demonstrated that the One-Pot Process reduced CO 2 emissions by 18 % and 27 % compared to traditional processes for SCP and indigoidine production, respectively. This study suggests a systematic design of the whole process for bioconverting full biomass components into bioproducts can simplify the pretreatment operations, enhance product yield, and reduce carbon emissions.
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高影响因子
APPLIED CLAY SCIENCE
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2024
·
IF 5.3
Jixing Fan,Qian Liu,Li,Li,Guanzheng Zhuang,Peng Yuan
Maya Blue, a hybrid pigment that can be synthesized by heating a mixture of palygorskite (or sepiolite) and indigo, has unclear relationships between the structural features of clay substrates, heating atmosphere, colors, and stability. This study investigates the color properties, stability, and clay-dye interactions of Maya Blue pigments based on palygorskite and sepiolite, heated under air and vacuum conditions (180 °C for 8 h). Reflectance spectra, CIE color parameters, surface properties, and Fourier-transform infrared spectroscopy were employed to evaluate these pigments. Results indicate that indigo monomers can penetrate the channels of both palygorskite and sepiolite, conferring a greenish-blue hue and high stability. However, the diffusion of indigo is determined by the size match between the channel and indigo monomers. In palygorskite, indigo monomers can diffuse only upon heating because the channel size is only a little bit larger than the indigo molecule, while in sepiolite, lager channels allow easy diffusion, even without heating. However, sepiolite's larger channels make indigo molecules more prone to chemical degradation or migration. Under vacuum conditions, indigo molecules more easily diffuse into the channels of both clay minerals, yielding pigments with higher saturation and enhanced stability. These findings highlight the size-matching between clay minerals' micropores and organic molecules and offer valuable guidance for optimizing the preparation process of clay-dye hybrid pigments.
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Arabian Journal of Chemistry
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2024
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IF 5.3
Changwei Zhang,Hua Yuan,Hong Shen,Chuan Li,Jianzhong Ye,Huaxing Zhang,Chengzhang Wang
The effect of acidolysis time, hydrochloric acid concentration, ozonization time and pH on the yield of indigo were investigated by single factor experiment using dried leaves of Baphicacanthus cusia (Nees) Bremek (DLB) as raw material. On this basis, the technological condition for preparing new indigo extract (NIE) by directional chemical conversion method was optimized by orthogonal experiment. Then, the physiochemical properties of NIE were analyzed by combustion assay, nitric acid assay, thin layer chromatography identification assay, moisture detection and water-soluble pigment observation assay. Moreover, UPLC-Q-TOF-MS/MS technology was utilized to compare chemical composition difference between the NIE and traditional indigo naturalis (TIN). Finally, NIE emulsion was prepared by ultrasound assisted high pressure homogenization method and its stability, antibacterial activity as well as antibacterial mechanism were evaluated. The experimental results showed that optimum process for preparing NIE were hydrochloric acid concentration of 2 %, ozonization time of 3 min, pH of 7 and acidolysis time of 1 h; Under this condition, the content and yield of indigo in the NIE were significantly improved, which could reach up to 42.6 ± 0.28 % and 2.26 ± 0.04 %, separately. NIE possessed similar physiochemical properties with TIN described in Chinese pharmacopoeia 2020, and had more diverse active ingredients than TIN. The prepared NIE emulsion demonstrated excellent stability, whether at dark or bright place, and no matter at low temperature (4 ℃) or room temperature (25 ℃), the particle size, PDI, pH and indigo content of which displayed little variation. In addition, the NIE emulsion owned good inhibitory activity against S. aureus , and its MIC and MBC values were 40 and 80 μg/mL, respectively. Moreover, NIE emulsion exhibited antibacterial effect by influencing cell membrane integrity, Ca 2+ -Mg 2+ -ATPase activity on the cell membrane and intracellular Ca 2+ levels of S. aureus. All of above results will lay the foundation for the green and sustainable utilization of Baphicacanthus cusia (Nees) Bremek.
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高影响因子
CLAYS AND CLAY MINERALS
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2025
·
IF 2.1
Li Li,Guanzheng Zhuang,Yanfu Wei,Mohammad Fahimizadeh,Peng Yuan
Sepiolite is considered a suitable substrate for Maya blue pigment. However, the interaction between sepiolite and indigo dye has not been fully understood. Previous studies have demonstrated that pre-treatment of sepiolite by heating or acid was useful in identifying the sepiolite–indigo interaction. The purpose of the present study was to prepare a series of hybrid sepiolite–indigo pigments after modifying the sepiolite using various alkali treatments (NaOH), then to evaluate their properties with respect to color, chemical resistance, and photostability. Samples were characterized using reflectance spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, transmission electron microscopy, and N2 adsorption-desorption. Under alkaline conditions, Si4+ and Mg2+ ions in sepiolite partially dissolved, disrupting the coordinated water associated with them. Mg2+ ions precipitated and blocked the structural channels of the sepiolite. The impact of the alkali treatment on the microporous structure and coordinated water of sepiolite significantly influenced the color properties and stability of the hybrid pigments. Proper alkaline treatment enhanced the greenish hue and chemical stability of the pigment, while severe treatments apparently compromised the structural integrity of the sepiolite, thus diminishing the quality of the hybrid pigment. Results from this study provide new insights into the color-causing and stabilizing mechanisms of sepiolite-based Maya blue pigment and also provide guidance for developing hybrid pigments based on clay minerals and organic dyes.
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高影响因子
Journal of Environmental Chemical Engineering
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2025
·
IF 7.2
Lingchao Kong,Qinyu Wang,Yeyin Zhang,Yi Wang,Chunmiao Zheng
The rapid expansion of the textile dyeing industry has created an urgent need for high-performance dye wastewater treatment materials due to the limitations of traditional adsorbents in efficiency and separability. Herein, we report the synthesis of ultralight, cost-effective aerogel adsorbents by introducing triethylenetetramine (TETA) into α-cellulose (CU). The resulting CU/TETA-0.25 aerogel displays outstanding adsorption capacities for Congo Red and indigo dyes, reaching 575.7±20 mg/g and 516.4±20 mg/g, respectively. Remarkably, less than 1 g of this aerogel continuously filters over 35 L (50 mg/L) of deeply colored red–blue dye wastewater to near transparency. Its high adsorption efficiency remains stable even under simultaneous exposure to multiple ions, organic compounds, and pH fluctuations. Mechanistic insights, derived from SEM, EDX, and XPS analyses and first-principles calculations (including molecular structure optimization, frontier molecular orbital distribution, weak interaction, and charge density difference), confirm that the –OH and –NH₂ groups on CU/TETA-0.25 drive dye adsorption through hydrogen bonding, supplemented by electrostatic and van der Waals interactions. Owing to its mass-producibility, lightweight nature, and low cost, CU/TETA-0.25 holds great promise for large-scale applications in mitigating water pollution caused by mixed dyes.
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Heliyon
·
2024
·
IF 3.4
Changwei Zhang,Hua Yuan,Hong Shen,Jianzhong Ye,Chengzhang Wang
In this study, Ginkgo biloba leave polyprenols (GBP) and polypyridine metal complex were individually utilized as functional ligand and main ligand, four kinds of novel GBP-based polypyridine metal complexes were successfully synthesized and their cell absorption capacity, light-dark stability, photodissociation efficiency, ROS production capacity, light-controlled antibacterial and anti-tumor activities as well as mechanisms were systematically investigated by ultraviolet visible spectrophotometer (UV–vis), confocal laser scanning microscope (CLSM), gel electrophoresis (GE), scanning electron microscope (SEM), oxford cup method, MTT method etc. The lipid water distribution coefficients of complex 1, 2 and 4 were all within the range of 0∼3, demonstrating their better cell absorption capacity and more competitive bioavailability potentiality compared with GBP. All of the synthesized complexes possessed excellent stability in a dark environment, and could conduct ligand dissociation under the condition of visible light irradiation except complex 1. In which, complex 2 and complex 4 were able to achieve degradation rates of 37.9 % and 54.4 % within 5 min, separately. In addition, complex 2 and complex 4 exhibited superior inhibitory activities on the HN-3 tumor cells on account of their stronger ROS production capacity. Moreover, the constricted expression of BCL-2 and NF-kB p-p65, especially the promoted expression of BAX may be one of the root cause. The four synthesized complexes had preferable inhibition effects against S. aureus under the condition of visible light irradiation in contrast to darkness, in which complex 4 was the best and its MIC and MBC values were 6.25 and 12.5 μg/mL, respectively. The antibacterial mechanism of the complex 4 may be in relation to the synergistic effect of multiple factors, including leakage of bacterial inclusion, change of cell membrane permeability and disruption of cell wall etc. All of the above generalized researches will pave a way for the high-value development and application of GBP-based functional products.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
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2025
·
IF 4.7
Li Li,Qian Liu,Guanzheng Zhuang,Jixing Fan,Peng Yuan,Fei Xu
Thermal treatment is a key step in synthesizing Maya blue pigments. While palygorskite-based Maya blue has been extensively studied, the preparation of sepiolite-based pigments remains underexplored. This study synthesized sepiolite-based Maya blue by heating indigo-sepiolite mixtures at 100–500 °C for 1–72 h, systematically investigating the effects of temperature and duration on pigment color and stability. Mechanistic insights were provided using XRD, surface area and micropore analysis, HRTEM, and FTIR. The optimal preparation conditions were identified as 100–200 °C with heating durations under 8 h. Below 200 °C, increasing temperature and time enhanced the greenish-blue hue and improved chemical and photostability due to the conversion of indigo oligomers to monomers and their diffusion into sepiolite channels. No evidence of strong interactions, such as hydrogen bonding or coordination, was found between indigo and sepiolite. Indigo encapsulation within sepiolite's channels likely explains its stability, while the conversion of intermolecular to intramolecular hydrogen bonds accounts for its coloration. Above 300 °C, indigo degradation caused pigment darkening. HRTEM showed no significant structural changes in sepiolite after heating. Sepiolite's nanostructure, microporosity, and diverse surface groups make it ideal for clay-dye hybrid pigments. These findings advance the understanding and application of sepiolite-based organic-inorganic hybrid materials.
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