Food Bioscience
·
2021
·
IF 4.24
Li Yuan,Yunfei Kong,Weijun Leng,Yanmin Wang,Wengang Jin,Ruichang Gao
The myosin aggregation of b.ighead carp ( Aristichthys nobilis ) induced by heating and the physical structure of surimi gels were changed using L-Glu. L-Glu increased the solubility of myosin regardless of heating or low/high ionic strength . Compared to myosin, the hydrophobicity of myosin with L-Glu at low ionic strength increased significantly, however there was no difference between myosin and myosin with L-Glu at high ionic strength. Atomic force microscopy and scanning electron microscopy results showed that L-Glu suppressed myosin aggregation, forming a honeycomb-like network after a two-step heating. Consequently, the suppression by L-Glu of myosin aggregation changed the physical properties of surimi gels. The water holding capacity of surimi gels with L-Glu increased significantly, but the hardness decreased. This study suggested that the influence of myosin aggregation induced using L-Glu increased ion-dipole interactions, hydrogen bonds and electrostatic repulsions, which changed the microstructure of surimi. L-Glu could be used as an additive to soften surimi gels with low salt for those with difficulty in chewing .
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Hongyu Zhou,Zhiwei Hu,Youming Liu,Shanbai Xiong
This study employs sensory evaluation, headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS), and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) techniques to investigate the effect of different pretreatment of fresh silver carp mince (running water rinsing 0, 1, or 2 times) and commercially frozen surimi on the odor characteristics of fish noodles. The free choice profiling (FCP) and check all that apply (CATA) sensory analysis methods were utilized to identify 10 characteristic descriptors, which include “grass, fish fragrance, unpleasant fishy, fatty, roast, ammonia, caramel, warmed-over, earthy, and mushroomy”. HS-GC-IMS and HS-SPME-GC-MS detected 80 and 37 volatile compounds (VCs) in fish noodles. The 1-Penten-3-ol, (E)-2-pentenal-D, hexanal-D, pentanal-D, (E,E)-2, 4-heptadienal-D contents were significantly correlated with “fish fragrance” and “unpleasant fishy”, and octanal, nonanal, heptanal, 2-methylpyrazine contents were significantly correlated with “warmed-over” flavor. The results of this study can be helpful for fish noodle quality improvement and industrial production.
研究领域:
食品科学
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化学分析
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Huifang Yang,Jing Qiu,Ramy M. Khoder,Shanbai Xiong,Ru. Liu,Yueqi. An
This study investigated the impact of chito-oligosaccharides (COS) on the odor characteristics of surimi gels and the adsorption capacity of myofibril protein (MP) to aldehydes. Surimi gels with 1 wt% COS exhibited the freshest odor and the least fishy note. Adding COS significantly reduced the relative content of total volatile compounds by 95.28 μg/kg-148.57 μg/kg (0.5 wt%-2 wt%). The partition coefficient ( K a / b i o p o l ) of aldehydes in 6 mg/mL MP and 1 wt% COS simulated decreased by 2.38% compared to the water system. It demonstrated that the interaction between COS and MP inhibited the release of aldehydes. Additionally, the content of COS greatly influenced the conformation of MP. At concentrations of COS up to 1 wt%, COS filling within the surimi gels created a compact network structure, increasing disulfide bonds and decreasing water interstices. This facilitated COS-MP-aldehyde interactions, with hydrophobic interactions pivotal in adsorbing fishy odors. The smaller quenching constant (Ksv) in the MP-fishy odorant-COS (MP-F-COS) simulated system indicated that COS directly reacted with aldehydes. However, higher COS concentrations (1.5–2 wt%) led to irreversible deformation of MP after being compressed, with aldehydes being embedded and absorbed by the mucous membrane. The present work offers novel perspectives on mitigating off-odor emission in surimi products by applying COS.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Mengzhe Li,Xin Wang,Tong Shi,Xiaoli Zhang,Zhiyu Xiong,Wengang Jin,Abdul Razak Monto,Li Yuan,Ruichang Gao
To investigate the effects of β-carotene on the gelling and digestion properties of surimi gels and the underlying molecular mechanisms, the gel properties, moisture distribution, rheological properties, secondary structure and microstructure were determined at different β-carotene concentrations (0 % ∼ 0.1 %). The results indicated that β-carotene levels from 0.02 % to 0.06 % were positively correlated with gel properties, and the storage modulus (G') gradually increased during heating. This was attributed primarily to the conversion of α-helix to β-sheet structures and the intensification of hydrophobic interactions, resulting in a more compact microstructure. Most importantly, at an additional level of 0.06 %, the digestibility did not significantly decrease with increasing gel properties. Moreover, the antioxidant activity of the hybrid gels subjected to gastrointestinal digestion was also enhanced. The present study provides a theoretical foundation for incorporating β-carotene into surimi which will provide more nutritious products.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
GLYCOCONJUGATE JOURNAL
·
2017
·Vol. 34
·期 5
·625-632
·
IF 2.186
Song Shuang,Yu Qi,Zhang Bao,Ai Chunqing,Sun Yujiao,Fu Yinghuan,Zhao Meiyu,Wen Chengrong
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%–115% with relative standard deviation of 3.0%–9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines.
研究领域:
食品科学
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分析化学
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营养学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Xu-Hui Huang,Rong Liu,Jia-Nan Chen,Xiu-Ping Dong,Bei-Wei Zhu,Lei Qin
Erythromycin has caused severe risk on the aquatic ecological environment and food chain propagation due to its extensive use, environmental persistence, and low efficiency of sewage removal. Our study monitored the erythromycin accumulation, self-detoxification behavior, and metabolic response of turbot in erythromycin-contaminated environments using a multi-omics method with the aim to improve the safety of turbot consumption and provide guidance for turbot culture. Erythromycin was found to rapidly accumulated and reached its peak in turbot in the first two days of exposure to erythromycin. Residual levels in the gill, liver, kidney, muscle, and blood were detected at 95.19, 72.80, 68.74, 51.3, and 12.33 ng/g, respectively. The risk of erythromycin residues in turbot can be effectively reduced by a withdrawal period longer than 12 d. Erythromycin-contaminated turbot muscles showed a significant decrease in flavor properties, particularly umami, sweet, and aroma precursors. A total of 232 lipids in four main categories, glycerides, phospholipids , fatty acyl, and sphingolipids, were found to show significant changes in the turbot muscles. The interacting metabolic network formed by taurine, glycerophospholipid, arginine, alanine , and glutamate metabolism, wherein turbot responded to erythromycin was verified through 14 significantly altered metabolites. A withdrawal period of at least half a month and the corresponding product labels for antibiotics should be formulated before the fish exposed to antibiotics during intensive culture are sold to protect the interests of consumers. This study could serve as a reference for the quality and safety risk assessment of turbot and other highly economical fishes affected by antibiotics exposure.
研究领域:
环境科学
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水产养殖学
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毒理学
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产品关联
DOI
高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 5
·191
·
IF 5.4
Haolong Zheng,Shiyang Gu,Shiqi Huang,Yan Zhang,Feng Xu,Daofei Lv,Wenbing Yuan,Kongyu Zhu,Xin Chen
The existing in vitro and clinical trial evidence supports the health and wellness benefits of collagen peptides sourced from various origins. Despite this, research on collagen peptides from tuna remains limited. Notably, tuna-derived peptides possess an inherent fishy odor, rendering them unsuitable for direct application in humans. This study explores the enhancement of tuna peptides’ applicability in cosmetics through odor mitigation. We developed a dual-phase ozone treatment, employing both dry and wet ozone, to deodorize tuna peptide powder, enabling its use in cosmetic formulations. The deodorized tuna peptide powder can be used in cosmetics. We optimized the ozone nitrification and deodorization conditions for tuna peptide powder by adjusting the treatment time, ozone concentration, and temperature. Sensory evaluation and GC-MS analysis confirmed the effectiveness of fishy odor removal, offering a comprehensive understanding of the deodorization process. The findings reveal that wet ozonation at 50 °C with an ozone concentration of 99.1 mg/L for 40 min significantly reduces the fishy odor of tuna peptide powder. Notably, n-Hexaldehyde, the primary odor-contributing volatile compound, decreased by 66.5%, confirming the efficacy of ozone treatment in odor mitigation. Moreover, the protein activity within the powder remained unaffected, ensuring the preservation of its functional properties. This study demonstrates the efficacy of ozone oxidation in adapting tuna peptide powder for cosmetic use.
研究领域:
生物化学
·
化妆品科学
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产品关联
DOI
PubMed
高影响因子
ZOOLOGICAL RESEARCH
·
2018
·
IF 0
Chao-Hao Chen,Bi-Jun Li,Xiao-Hui Gu,Hao-Ran Lin,Jun-Hong Xia
Table 1 Summary information on sex reversal of the F1 generation Group DES (g)/feed (kg) Individual number ( n ) Female Male Intersex Female rate (%) Group1 1 330 330 0 0 100 Group2 0.5 390 371 6 13 95 Control No DES 636 190 446 0 30 Total N/A 1 356 891 452 13 N/A Open in a separate window N/A: Not available.
研究领域:
水产养殖学
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遗传学
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产品关联
DOI
PubMed
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 18
·18832-18841
·
IF 2.76
Li Qian,Wang Peipei,Chen Ling,Gao Hongwen,Wu Lingling
Zebrafish ( Danio rerio ) embryos and larvae were selected to investigate the potential risk and aquatic toxicity of a widely used pharmaceutical, naproxen. The acute toxicity of naproxen to embryos and larvae was measured, respectively. The histopathology was investigated in the liver of zebrafish larvae after 8-day embryo-larvae exposure to naproxen. The values of 96-h LC 50 were 115.2 mg/L for embryos and 147.6 mg/L for larvae, indicating that zebrafish embryos were more sensitive than larvae to naproxen exposure. Large suites of symptoms were induced in zebrafish ( D. rerio ) early life stages by different dosages of naproxen, including hatching inhibition, lower heart rate, and morphological abnormalities. The most sensitive sub-lethal effect caused by naproxen was pericardial edema, the 72-h EC 50 values of which for embryos and larvae were 98.3 and 149.0 mg/L, respectively. In addition, naproxen-treated zebrafish larvae exhibited histopathological liver damage, including swollen hepatocytes, vacuolar degeneration, and nuclei pycnosis. The results indicated that naproxen is a potential threat to aquatic organisms.
研究领域:
水生毒理学
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药理学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Shaolin Xie,Bing Yang,Siying Li,Liangjun Ge,Min Li,Qingshi Chen,Xian Qing,Jixing Zou
CYP1A is the most commonly used biomarker and transgenic fish which carrying a cyp1a promoter to drive a reporter gene can be used as reliable way to monitor dioxin/dioxin-like compounds (DLCs) in the environment. Here, we cloned the cyp1a promoter of Gambusia affinis and this promoter showed stronger transcriptional activity than that of zebrafish. Then, a Tg(GAcyp1a:eGFP/Luc) transgenic zebrafish line was first constructed with the G. affinis cyp1a promoter driving eGFP expression using meganuclease I-SceI mediated transgenesis technology. The Tg(GAcyp1a:eGFP/Luc) larvae at 72 h post-fertilization (hpf) were tested by exposing to TCDD for 72 h, and induced GFP was mainly expressed in the liver with low background. The Tg(GAcyp1a:eGFP/Luc) zebrafish showed high sensitivity (limit of detection of 0.322 ng/L TCDD and 0.7 TEQ-ng/L PCDD/Fs) and specificity (insensitive to responses to PAHs and PCBs). In addition, the transgenic line showed a low detection concentration of the DLCs contaminated environmental samples (as low as 1.8 TEQ-ng/L), and the eGFP fluorescence intensity and the chemical-TEQ values were closely correlated. In conclusion, a sensitively and specifically transgenic zebrafish line was established to convenient and effective to detect DLCs in the environment.
研究领域:
环境科学
·
分子生物学
·
毒理学
·
遗传学
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2023
·
IF 3.8
Yi Zhang,Wenzheng Li,Jun Zhu,Xin Qian,Wenlong Pei,Zhenyang Gu,Qian Wu,Zhan Zhang,Lei Li
Pentachlorophenol (PCP) is a ubiquitous environmental toxicant with various adverse effects. Although its neurotoxicity has been reported, the underlying mechanism and subsequent detoxification remain unclear. In this study, embryos and adult zebrafish were exposed to PCP to determine its potential neurotoxic mechanism and protective indicators. The survival rate, heart rate, mobility time, active status and moving distance were significantly decreased in larvae after 30 μg/L PCP exposure. Likewise, the mobile time, latency to the first movement, velocity and moving distance of adult zebrafish were significantly reduced by PCP exposure. Untargeted metabolomics analysis of larvae revealed that arginine and proline metabolism was the primary pathway affected by PCP exposure, reflected by increased proline and decreased citrulline (CIT) contents, which were confirmed by quantitative data. PCP exposure suppressed the conversion from arginine to CIT in larvae by downregulating the expression of nos1 and nos2a . Ornithine content was increased in the brains and intestines of adult zebrafish after PCP exposure, which inhibited ornithine catabolism to CIT by downregulating otc , resulting in reduced CIT. Intriguingly, CIT supplementation significantly restored the neurobehavioral defects induced by PCP in larvae and adult zebrafish. CIT supplementation upregulated the expression of ef1α and tuba1 in larvae and inhibited the downregulation of ef1α in the brains of adult zebrafish. Taken together, these results indicated that CIT supplementation could protect against PCP-induced neurotoxicity by upregulating the expression of genes involved in neuronal development and function.
研究领域:
毒理学
·
神经科学
·
代谢组学
·
斑马鱼生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 10
·e15811
·
IF 2.19
Cikun Liu,Wenxie Li,Boyan Lin,Shumin Yi,Beibei Ye,Hongbo Mi,Jianrong Li,Jinxiang Wang,Xuepeng Li
This study investigates the effects of ozone water rinsing on the myofibrillar protein (MP) oxidation, color, and aroma characteristics of grass carp surimi. The results showed that the incubation of surimi with increased concentrations of ozone water led to a gradual generation of MP carbonyl derivatives and a loss of sulfhydryl. Additionally, the surface hydrophobicity of the MP increased, accompanied by a loss in tryptophan residues. The cross-linking via disulfide linkages and aggregates of the MP was formed after ozone water rinsing. The surimi treated with 3, 5, 7 and 10 mg/L ozone water had higher L* and whiteness values than that of the control group. After rinsing with ozone water, the relative contents of main volatile compounds including hexanal, heptanal, and 1-octen-3-ol declined significantly. The results demonstrated that rinsing with ozone water can improve the sensory quality of grass carp surimi, and also lead to MP oxidation. Novelty impact statement Rising surimi with ozone water is an effective method to improve the color property of freshwater fish surimi. The work provides detailed information about the effects of ozone water on protein oxidation, whiteness, and volatile compounds of grass carp surimi. This not only contributes to perform a reasonable assessment for scientists to the use of ozone water in surimi production, but also give a reference for the production of high-quality surimi.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）