SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Mengke Wang,Yaqing Han,Rui Huang,Zhonghao Wang,Guannan Wang
Fluorescent gold nanoclusters (AuNCs) have generated significant research interest because of their fascinating physicochemical properties . However, their biosensing applications are limited by the relatively low quantum yield (QY). Herein, a simple confinement effect based on AuNCs as guest molecules and Zn-containing hydroxy double salt (HDS) as host material (AuNCs@HDS) was used to significantly improve the fluorescence properties of AuNCs. The QY increased from 1.24 % to 22.80 %, and the time-tedious preparation or purification procedures were avoided. Further, the validated excellent fluorescence properties of AuNCs enabled the construction of a versatile fluorescent sensing strategy for bio-enzymes for the first time. The p-nitrophenol (pNP), a product of many bio-enzymes-catalyzed hydrolyzes of pNP-substrate, can effectively quench the fluorescence of AuNCs@HDS through an inner filter effect (IFE) process. Therefore, the AuNCs@HDS successfully detected several enzymatic disease biomarkers with robust analysis capabilities by varying the pNP-substrate, including alkaline phosphatase (ALP), β-glucosidase (β-GLU) and β-galactosidase (β-GAL). This study will inspire the design of highly fluorescent AuNCs as tools for biosensing.
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ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Lai Liang,Zhang Mengyun,Liu Chusheng,Qu Jiahuan,Xu Dongsheng,Jiang Zhengjin
Rapid and effective separation of nucleotides (NTs) and their derivatives is crucial for studying their physiological functions. In this work, we comprehensively evaluated the separation ability of a zwitterionic hydrophilic monolith, i.e., poly(N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl)ammonium betaine- co –N,N’-methylenebisacrylamide) (poly(SPP- co -MBA)) for NTs analysis, including its selectivity, chemical stability under extremely basic condition and compatibility with hydrophilic interaction liquid chromatography (HILIC) coupled with mass spectrometry (HILIC-MS). The poly(SPP- co -MBA) monolith exhibited excellent chemical stability, as evidenced by the low relative standard deviation of retention time (0.16–1.05%) after 4000 consecutive injections over one month under strong alkaline elution condition (pH 10). After optimizing the separation conditions, including buffer pH and concentration, organic solvent content and column temperature, four nucleoside triphosphates, five nucleoside diphosphates and five nucleoside monophosphates were baseline separated within 7 min. Additionally, the mixtures containing one nucleoside and its corresponding mono-, di-, and triphosphates were baseline separated within only 3 min, respectively. It is good HILIC-MS compatibility was also confirmed by the satisfactory peak shape and high response of nine NTs. Overall, the proposed poly(SPP- co -MBA) monolith exhibited good mechanical stability and compatibility of HILIC-MS, making it a promising technique for NTs analysis. Graphical Abstract
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 21
·2300090
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IF 3.1
Qiangqiang Jia,Zufan Yang,Qian Wang,Haishan Yang,Xiaofeng Tang,Hongyang Zhang,Lingling Cao,Gong Zhang
It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra-high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15 min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R 2 ≥ 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values ≤ 9.3%), and precise (the intra- and inter-day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin-intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.
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FOOD CHEMISTRY
·
2023
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IF 8.8
Meng Dong,Feng Liang,Shuang Cui,Bing-Bing Mao,Xu-Hui Huang,Lei Qin
The effect of steaming treatment on salmon quality was explored by different multi-omics and electronic sensory system in this study. A comparison between conventional steaming (CS) and anaerobic steaming (AS) was conducted in organoleptic quality of salmon. Twelve key volatile compounds were identified, which contributed to the flavor difference. The concentrations of hexanal, (E)-2-octen-1-al, and decanal in AS salmon were significantly lower than in CS salmon, which account for 68.9–80.5 % of the latter. During steaming, the fatty acids and diacylglycerols decreased significantly by 37.4 % and 57.9 %, respectively. Anaerobic steaming limited the degradation of some oxidized lipids, further reduced some volatile secondary oxidation products. Nucleotides and derivatives, succinic acid, glutamic acid, hydroxyproline and betaine contributed to the saltness, umami, richness of steamed salmon. Metabolomics data revealed that the higher creatinine, Ala-Ala and Ala-Leu provided more umami and less bitterness to AS salmon.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Houmei Liu,Pian Jin,Mi Jiang,Yu Duan,Guozheng Zhu,Hui Yu,Hongdeng Qiu
In this work, for the first time five amine-ligands including mono-amine, di-amine, tri-amine, secondary and tertiary amine, were functionalized on mesoporous micro-silicas and developed as stationary phases for hydrophilic interaction liquid chromatography (HILIC). The investigations about the retention mechanisms, effects of different chromatographic conditions and stability were systematically conducted. Three kinds of polar and hydrophilic compounds (saccharides, sulfonamides, nucleosides and nucleobases) were selected as probe molecules to evaluate their separation performances. Among the five stationary phases, only aminopropyl-bonded silica has already gained wide developments and applications. Whereas, there are no related researches about the other four to be utilized as separation media. By a series of chromatographic evaluations, the results revealed the other four mesoporous micro-silica materials functionalized with di-amine, tri-amine, secondary and tertiary amine, had great potential to be explored as novel stationary phases of HILIC. Particularly, the two stationary phases functionalized with di-amine and tri-amine exhibited outstanding separation and retention abilities. This work offered some insights on the understanding of retention in HILIC mode and provided us possibility to explore other amine-based HILIC stationary phases.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Xiang Wang,Xin Yan,Bin-cheng Wang,Wei Xue,Chang-ren Zhou,Yi Zhang,Zong-hua Liu
Blood lead poisoning is a universal and severe health problem that greatly threatens human health in various industries. Elimination of blood lead relying on chelating agents and combination with hemoperfusion adsorbents has achieved considerable progress, but it is still suffering from the compromised selectivity of adsorbents as well as in absence of real time monitoring during treatment. Herein, we proposed a selective blood lead adsorbent integrated with real-time visualized/electrochemical bi-modal monitoring based on TMPyP-bound guanosine-borate (GB) supramolecular hydrogel as potential smart hemoperfusion device. The GB hydrogel possessed stability in physiological environment, self-healing ability resistant to fluid shear, blood compatibility, selective adsorption of lead ions superior to conventional adsorbents, anti-fouling performance to blood components and renewability. Benefiting from binding with TMPyP and the intrinsic conductivity, GB hydrogel was endowed with the ability to qualitatively diagnose the presence of blood lead via simple color change and quantitatively reflect the amount of adsorbed lead from blood accurately through electrochemical technique . This work puts forward an integrated treatment/monitoring hemoperfusion device with high selectivity, simple fabrication and low-cost, providing a paradigm for next generation design of intelligent, monitorable theranostic hemopurification system, which is also an extensible platform for the other research fields such as environmental monitoring and remediation.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 4
·1383-1391
·
IF 2.647
Yang Hongmei,Hu Peiyu,Tang Jing,Cheng Ying,Wang Fang,Chen Zilin
A simple and sensitive bifunctional electrochemical aptasensor for detection of adenosine and thrombin has been developed using gold nanoparticles–electrochemically reduced graphene oxide (AuNPs-ERGO) composite film-modified electrode. Firstly, the reduced graphene oxide film and AuNPs were sequentially immobilized on glassy carbon electrode (GCE) surface. Secondly, thrombin aptamer was immobilized on the modified electrode. Finally, adenosine aptamer was hybridized with it to serve as a recognition element and methylene blue (MB) as electrochemical signal indicator. In the presence of adenosine or thrombin, the sensor recognized it and a conformational change was induced in aptamer, resulting in decrease of the peak current of MB. The linear relation between concentration of adenosine or thrombin and peak current of MB allowed quantification of them. Thanks to the special electronic characteristic of AuNPs-ERGO composite film, sensitivity of sensor was greatly improved. Under optimal conditions, the proposed aptasensor presented an excellent performance in a linear range of 25 nM to 750 nM for adenosine and 0.5 nM to 10 nM for thrombin. Detection limits were estimated to be 8.3 nM for adenosine and 0.17 nM for thrombin, respectively. Moreover, dual-analyte detection of adenosine and thrombin was achieved without potentially increasing the complexity and cost of the assay.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuhong Xia,Tian Lu,Liping Wang,Jinling Mo,Yang Jin,Liying Zhang,Shuhu Du
Methylglyoxal (MGO) is the primary material basis for the non-peroxide antibacterial activity (NPA) of manuka honey from New Zealand. Therefore, it is necessary to identify the quality or discriminate the grade of honey because no all manuka honeys on the market display the NPA. The current routine method employed for the detection of MGO involves high-performance liquid chromatography (HPLC) test. However, it requires long time (∼8 h) for sample derivatization . Herein, we report an intrinsic Raman signal amplification strategy for the rapid identification and detection of MGO by using silver-coated gold nanoparticles (Au@Ag NPs) along with a high selective surface-enhanced Raman scattering (SERS) probe 8-thioguanosine (8-TG). 8-TG is synthesized via the derivatization of 8-bromoguanosine (8-BG) with thiourea , and its Raman peak assignments were confirmed by computer simulation. The detection is performed through the Raman intensity ratio (I 631 /I 700 ) variation of N 2 -(1-carboxyethyl)-thioguanosine (CETG) formed by the reaction between 8-TG and MGO on surface of Au@Ag NPs, where one CETG Raman intensity at 631 cm −1 increases while the other one at 700 cm −1 decreases oppositely. The opposite change not only yields an intrinsic Raman signal amplification, but also provides built-in correction. As a result, the proposed SERS method exhibits high sensitivity and accuracy. In addition, the whole analytical test is achieved within ∼20 min. The method can be used for the fast detection of MGO in manuka honey and discrimination of the honey grade.
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Analytical Methods
·
2020
·Vol. 12
·期 39
·4789-4797
·
IF 2.596
Jiawei Li,Zhengfan Shui,Liang Dong,Lian Shen,Dong Zhao,Huibo Luo,Yi Ma,Changjun Hou,Danqun Huo
The organic acid content plays important roles in the flavor and taste of Chinese baijiu. Developing a detection and discrimination technique for organic acids and employing it as a basis in baijiu classification has great practical significance. We employed 3 kinds of acid-sensitive quantum dots (QDs) to construct a fluorescence sensor array for the detection and identification of organic acids in baijiu. We report the first directional use of array sensing detection technology for the evaluation of organic acids in baijiu. Linear discriminant analysis (LDA) was successfully employed to evaluate the ability of the as-developed sensor array to classify organic acids. The Euclidean distance analysis was introduced to prove the provided sensor array possesses good quantitative detection. On this basis, our sensor array was successfully applied to distinguish 16 kinds of baijiu samples. The results were supported by principal component analysis (PCA), LDA, and systematic cluster analysis (HCA). Furthermore, Pearson correlation results indicated a strong correlation between the detection results and the organic acids in baijiu. This simple and accurate method shows potential for quality control and detection in baijiu factories and markets.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Jincai Wang,Jialiang Guo,Haowen Chen,Xiaoling Huang,Govert W. Somsen,Fenyun Song,Zhengjin Jiang
A single-step copolymerization strategy was developed for the preparation of carbohydrate (glucose and maltose) functionalized monoliths using click reaction. Firstly, novel carbohydrate-functionalized methacrylate monomers were synthesized through Cu(I)-catalyzed 1,3-dipolar cycloaddition (alkyne-azide reaction) of terminal alkyne with azide of carbohydrate derivatives. The corresponding carbohydrate functionalized monolithic columns were then prepared through a single-step in-situ copolymerization. The physicochemical properties and performance of the fabricated monolithic columns were evaluated using scanning electron microscopy, Fourier-transform infrared spectroscopy, and nano-liquid chromatography. For the optimized monolithic column, satisfactory column permeability and good separation performance were demonstrated for polar compounds including nucleoside, phenolic compounds and benzoic acid derivatives. The monolithic column is also highly useful for selective and efficient enrichment of glycopeptides from human IgG tryptic digests. This study not only provided a novel hydrophilic column for separation and selective trapping of polar compounds, but also proposed a facile and efficient approach for preparing carbohydrate functionalized monoliths.
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Analytical Methods
·
2020
·Vol. 12
·期 46
·5635-5641
·
IF 2.596
Dan Liu,Lili Meng,Liping Huang,Shipeng Luo,Guohua Zhang
Chitosan is a considerably versatile and promising biomaterial and can easily form a 3D hierarchical porous scaffold. In this work, a novel boronate-affinity monolithic column modified with a boronic acid–chitosan complex was prepared and characterized by different methods such as Fourier transform infrared spectroscopy, scanning electron microscopy, thermal gravimetric analysis, specific surface area analysis and pore size distribution analysis. The synthesized monolithic column was used for polymer monolithic microextraction combined with high performance liquid chromatography for the simultaneous determination of cytidine, uridine, inosine, and guanosine in milk powder samples. Several parameters affecting extraction efficiency, including the eluent proportion, eluent flow rate, sample flow rate, sample volume, and sample pH were investigated. The boronate-affinity monolithic column showed high enrichment ability due to the selective formation of cyclic borate esters between nucleosides and boronic acid groups at high pH and the release of cyclic borates at low pH values. Under the optimum operating conditions, the linear range was 0.1–50 μg mL−1, and the correlation coefficients were in the range of 0.9993–0.9994. The LOD and LOQ were in the range of 0.0027–0.0034 and 0.0090–0.011 μg mL−1, respectively. In addition, the results of recovery and relative standard deviation were satisfactory.
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APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Di Zhu,Huiyuan Ma,Qingfang Zhen,Jianjiao Xin,Lichao Tan,Chunjing Zhang,Xinming Wang,Boxin Xiao
Epinephrine (EP) is a significant neurotransmitter, energizes and maintains the various body systems and plays a pivotal role in the response to stressful conditions of blood pressure and blood sugar level. Herein, a novel epinephrine sensor based on the hierarchical flower-like zinc oxide nanosheets (flower-like ZnO) embedded into three-dimensional ferrocene-functionalized graphene framework (3D graphene@Fc) was successfully prepared, using a facile route of one-pot in situ hydrothermal growth method. Benefiting from the unique structural advantages, meanwhile integrated with synergistic effect of components, the flower-like ZnO/3D graphene@Fc exhibits excellent sensing performance for epinephrine with a wide linear range of 0.02 to 216 μM, a low detection limit of 0.0093 μM, high anti-interference and good cyclic stability. Interestingly, it is unexpectedly found that the flower-like ZnO/3D graphene@Fc has sensing ability for EP’s oxidized derivative (QA) during the oxidation process of EP, along with a linear range of 0.192 to 527 μM and a detection limit of 0.1 μM for QA. More importantly, acceptable recovery obtained from the real sample test, confirming the feasibility of this biosensor for practical application.
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