BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Xiaojian Li,Yaoguang Wang,Li Shi,Hongmin Ma,Yong Zhang,Bin Du,Dan Wu,Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
研究领域:
生物传感
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Yang Gao,Weishuang Fang,Tianyi Xie,Haiyi Peng,Xiaogang Yao,Huixing Lin
Mg 1–3 x (CoNiZn) x Ta 2 O 6 ceramics ( x = 0.00, 0.05, 0.10, 0.20, and 0.25) were synthesized by the solid-phase reaction method. The XRD and Rietveld refinement results suggest that the Mg 1–3 x (CoNiZn) x Ta 2 O 6 ceramics have a pure tri-rutile structure. (Co 1/3 Ni 1/3 Zn 1/3 ) 2+ can significantly broaden the sintering temperature range of ceramics. The chemical bonding theory reveals that the A-O bond (A = Mg, Co, Ni, or Zn) ionicity and Ta-O bond ionicity are closely related to the porosity corrected relative permittivity ( ε r -corr. ). The increase in A-O bond energy contributes to the greater stability of the AO 6 octahedron , resulting in a decrease in | τ f |. Additionally, the Raman spectroscopy reveals that there is a negative relationship between the Qf value and the full width at half maximum ( FWHM ) of the strongest peak of the A 1g mode. Correspondingly, Mg 0.4 (CoNiZn) 0.2 Ta 2 O 6 ceramic has excellent microwave dielectric properties after sintering at 1450°C for 4 h: ε r = 28.9, Qf = 91,900 GHz (at 7.4 GHz), and τ f = 44 ppm/°C. Furthermore, Mg 0.4 (CoNiZn) 0.2 Ta 2 O 6 ceramic can maintain a stable Qf value (∼ 91,000 GHz) across a broad range of sintering temperatures (150°C), which is a result of the combination of densification and FWHM . This characteristic facilitates adaptation to changes in furnace temperature in large-scale production. The strategy of multiple-element substitution to extend the sintering temperature range is expected to be applied to other ceramic systems.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
DOI
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 7
·1588
·
IF 4.2
Ke Chen,Difan Deng,Yupeng Yin,Dongmei Xi,Phumbum Park,Wei Gao,Rui Liu,Kang Lei
To identify fungicide lead compounds with novel scaffold and high efficacy, a library of 4,5-dihydrotetrazolo [1,5-a]thieno [2,3-e]pyridine derivatives, consisting of 10 newly synthesized compounds and 12 previously reported compounds, was evaluated for their potential as fungicide agents. In vitro bioassay results indicated that some target compounds exhibited certain antifungal activity against the tested fungi at a concentration of 50 μg/mL. Especially, compoundsI-1,I-5,I-7, andI-12demonstrated promising antifungal activity againstC. arachidicola,R. solani, andS. sclerotiorum, with EC50values ranging from 4.61 to 6.66 μg/mL. Additionally, transcriptome analysis revealed that the molecular mode of action of compoundI-12involves the inhibition of nitrogen metabolism and the proteasome pathway. The present work demonstrates that 4,5,6,7-tetrahydrothieno [3,2-b] pyridine represents a promising lead scaffold and provides important theoretical foundations and innovative perspectives for the development of novel and highly efficient fungicides.
研究领域:
植物病理学
·
农药化学
·
生物信息学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xueqing Gao,Xiaowen Gu,Qi Min,Yueyue Wei,Chunyuan Tian,Xuming Zhuang,Feng Luan
In this work, we demonstrate a novel solid-state electrochemiluminescence (ECL) sensor based on the Ru(bpy) 3 2+ @terbium-guanosine monophosphate infinite coordination polymer network ((Ru(bpy) 3 2+ @Tb-GMP ICPn). Comparing with the traditional luminescence of Ru(bpy) 3 2+ observed in a liquid system, the proposed method of encapsulating Ru(bpy) 3 2+ into ICPn for immobilization greatly improves the ECL signal and efficiency, which is attributed to the unique porous structure and large specific surface area of ICPn. Moreover, the solid-state Ru(bpy) 3 2+ ECL sensor has good biocompatibility and low toxicity. Taking histamine (HA) as a detection model, a good linear relationship between ECL intensity and logarithm of HA concentration was obtained with a low detection limit of 17 nM, and satisfactory results were obtained for detecting HA levels in fish samples as well. The proposed solid-state Ru(bpy) 3 2+ ECL sensor has great application prospects in the safety of food.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2014
·Vol. 38
·期 7
·3078-3083
·
IF 3.159
Dan Kong,Yanyan Liu,Juan Zhang,Hongbian Li,Xiangyu Wang,Guangyu Liu,Baojun Li,Zheng Xu
The research on the heterogeneous catalytic synthesis of tetrazoles has attracted great attention. In this paper, a tungsten atom-containing AlPO-5 microporous molecular sieve (termed as WAlPO-5) was designed and synthesized by the incorporation of tungsten atoms into the AlPO-5 skeleton with triethylamine as a structure template from pseudoboehmite, H3PO4 and tungstophosphoric acid. The incorporation of tungsten atoms into the AlPO-5 skeleton has been demonstrated by systematic characterizations. WAlPO-5 can be used as an efficient heterogeneous catalyst for the synthesis of 5-substituted 1H-tetrazoles by [3+2] cycloaddition from nitriles and sodium azide. As a novel heterogeneous catalyst, WAlPO-5 exhibits a high catalytic activity, a superior cycling stability and an excellent substrate applicability. The significant advantages of WAlPO-5, such as the simple procedure and the mild reaction conditions and as an alternative to those corrosive, hazardous and polluting homogeneous catalysts, warrant its potential application in industrial processes.
研究领域:
化学
·
催化科学
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产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2014
·
IF 3.432
Jing Wu,Linxi Yu,Feifei Yang,Jingjie Li,Peng Wang,Wenbo Zhou,Liwen Qin,Yunqi Li,Jian Luo,Zhengfang Yi,Mingyao Liu,Yihua Chen
A series of 2,3-diaryl-4-thiazolidinone derivatives were synthesized and evaluated for their antiproliferative properties against two well-known cancer cell lines (A549 as human lung cancer and MDA-MB-231 as human breast cancer). Structure activity relationship (SAR) analysis resulted in the discovery of 2-(3-(arylalkyl amino carbonyl)phenyl)-3-(2-methoxy-phenyl)-4-thiazolidinone derivatives with high potent inhibitory effects on the proliferation of both cancer cell lines. Furthermore, several compounds with potent antiproliferative activities displayed excellent inhibitory activities on migration with an IC 50 of about 0.05 μM on MDA-MB-231 cells in two different migration assays. In particular, compound 39 was indicated to suppress tumor growth and metastasis as well as promote survival rate. Intriguingly, this series of analogs have been indicated to inhibit tumor cell proliferation through inducing cell cycle arrest . These results suggested that the new series of 2-(3-(arylalkyl amino carbonyl)phenyl)-3-(2-methoxyphenyl)-4-thiazolidinone derivatives could be regarded and developed as novel highly potential anticancer agents in the future.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Kaida Kuang,Ya Li,Yu Ji,Yang Liu,Nengqin Jia
In this work, a quenched molecularly imprinting-electrochemiluminescence (MIP-ECL) sensor based on Ru(bpy) 3 2+ @ZnO-Au nanocomposites was constructed to realize the sensitive detection of acrylamide (AM). Ru(bpy) 3 2+ @ZnO-Au nanomaterials show high ECL efficiency. AuNPs has excellent properties and can improve the properties of electrochemiluminescence by promoting the electron transfer process of the luminescent body. The MIP polymer film was formed on the surface of the material and eluted with eluent, leaving an AM imprinting cavity as a specific recognition site. When adding different concentrations of AM, it was specifically recognized by the MIP membrane, and the ECL signal decreased gradually, thus realizing the sensitive detection of AM. Under the optimal conditions, the detection ranges of the MIP-ECL sensor are 1–10 8 nM and the limit of detection (LOD) is 0.123 nM. Therefore, this sensor proposed in this work opens up a new way for the sensitive detection of AM in food.
研究领域:
分析化学
·
食品安全检测
·
纳米材料科学
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2020
·Vol. 43
·期 5
·831-838
·
IF 2.419
Liu Bin,Wang Xufeng,Zhao Jiahao,Qin Liehao,Shi Lei,Zhou Tao,Liu Shuang,Wang Chunling
Zygosaccharomyces rouxii referred as Yeast S is an important microorganism widely applied in traditional fermented food to accelerate flavor formation. Z. rouxii 3-2 referred as Yeast S 3-2 constructed previously is a new strain having higher salt tolerance than wild type. In this study, salt stress response of synthesis key flavor metabolites 3-methylthiopropanol, 2-phenylethyl alcohol, and isoamyl acetate in Yeast S and S 3-2 were investigated based on SPME–GC–MS and RT-qPCR. Analysis of GC–MS data showed that high salinity led to increase the contents of 3-methylthiopropanol and 2-phenylethyl alcohol both in Yeast S and Yeast S 3-2, while inhibited the synthesis of isoamyl acetate, and the induced effect on Yeast S 3-2 was more pronounced than Yeast S. Furthermore, the RT-qPCR results revealed that the salinity could enhance the genes expression of ARO10 and PDC1 , and stronger effect on Yeast S 3-2 than S. However, the gene expression of AFT1 was reduced in high-salt culture conditions, which was consistent with the results of GC–MS data. The results presented in this study can provide theoretical support of Z. rouxii 3-2 application during food fermentation.
研究领域:
微生物学
·
食品科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2024
·Vol. 22
·期 14
·2764-2773
·
IF 2.9
Yue Song,Wenjie Fan,Chen Yao,Heng Wang,Xiuxiang Lu,Yumin Wang,Pengxiang Liu,Yanjie Ma,Zhen Zhang,Jiang Wang,BeiBei Chu,Lijun Shi,Guoyu Yang,Mengdi Wang
Pattern recognition receptors (PRRs) play a critical role in the innate immune response, and toll-like receptor 7 (TLR7) is an important member of PRRs. Although several TLR7 agonists are available, most of them are being tested clinically, with only one available on the market. Thus, it is imperative to develop new TLR7 agonists. In this study, we designed and synthesized three kinds of quinazoline derivatives and five kinds of pyrrolo[3,2-d]pyrimidine derivatives targeting TLR7. The antiviral efficacy of these compounds was evaluated in vitro and in vivo. Our findings indicated that four kinds of compounds showed exceptional antiviral activity. Furthermore, molecular docking studies confirmed that compound 11 successfully positioned itself in the pocket of the TLR7 guanosine loading site with a binding energy of −4.45 kcal mol−1. These results suggested that these compounds might be potential antiviral agents.
研究领域:
免疫学
·
药物化学
·
病毒学
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Daifang Wang,Longhua Guo,Rong Huang,Bin Qiu,Zhenyu Lin,Guonan Chen
Electrochemiluminescence (ECL) of Ru(bpy) 3 2+ has been widely used in analytical chemistry . Herein, we present a new approach to enhance the ECL of Ru(bpy) 3 2+ by the localized surface plasmon resonance (LSPR) of gold nanorods (AuNR). Our investigations reveal that the ECL intensity could be greatly enhanced by controlling the distance between Ru(bpy) 3 2+ and the surface of AuNRs. We call this kind of surface plasmon induced ECL enhancement as surface-enhanced electrochemiluminescence (SEECL). This kind of SEECL phenomenon is utilized to fabricate a biosensor for ultrasensitive Hg 2+ detection. The SEECL biosensor is fabricated by self-assembling AuNRs and T-rich ssDNA probes on gold electrode surface. With the presence of Hg 2+ , the conformation of ssDNA probes changed to be hairpin-like structure via formation of T-Hg 2+ -T structure. And Ru(bpy) 3 2+ could insert into the grooves of the hairpin structured DNA probes to generate ECL emission, which could be enhanced by the LSPR of AuNR. The ECL intensity of the sensor increased with the concentration of Hg 2+ , and a detection limit of 10 fM Hg 2+ in aqueous solution was achieved. The effect of different LSPR peak location of AuNR on the sensitivity of biosensor has been investigated. The results show that a good overlap between the LSPR absorption spectrum and the ECL emission spectrum of Ru(bpy) 3 2+ could achieve the best ECL signal enhancement.
研究领域:
分析化学
·
生物传感
·
纳米技术
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Xinyi Ni,Yuan Zhao,Qijun Song
An integrative method based on the combination of electrochemical reduction (ECR) and in-situ electrochemiluminescence (ECL) detection was developed for the sensitive detection of the nitroaromatic compounds (NACs), including 4-nitrotoluene (4-NT), 2,4-dinitrotoluene (2,4-DNT), 2,4,6-trinitrotoluene (TNT). An electrochemical flow cell including two working electrodes was fabricated with poly(dimethylsiloxane) (PDMS) to achieve the dual functions. The first one is a copper mesh electrode, acting as the reductor to convert the NACs to aminoaromatic compounds (AACs). Electrochemical deposition of copper nanoparticles on the surface of the copper mesh was conducted to further increase the reduction efficiency. The second one is an ECL electrode, which was used for in-situ detection of the reduced products. The ECL electrode was fabricated by the immobilization of Ru(bpy) 3 2+ on a glassy carbon electrode (GCE) with the Nafion/the multi-wall carbon nanotubes (MWCNTs) composite film. The copper mesh electrode was arranged directly underneath the ECL electrode to ensure the reduced products could be immediately detected in-situ. An electrolytic solution consists of 33.3 mmol/L Na 2 SO 4 and 16.7 mmol/L phosphate buffer solution (PBS, pH7.0) was found to be optimum for both the electrochemical reduction of the NACs and the ECL detection. The ECL intensities were proportional to the concentrations of 4-NT, 2,4-DNT and TNT in the range from 1.0 × 10 −9 to 1.0 × 10 −5 , 3.5 × 10 −9 to 1.0 × 10 −5 and 7.5 × 10 −9 to 1.0 × 10 −5 mol/L respectively. The limit of detections (LOD) were 4.0 × 10 −10 , 1.7 × 10 −9 and 3.1 × 10 −9 mol/L respectively for 4-NT, 2,4-DNT and TNT. The reduction mechanism was preliminarily investigated and it was found that the presence of oxygen was critical for the reduction process, suggesting that the reductive oxygen species O 2 − , O H could be involved in the NACs reduction process.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Libo Li,Wanlin Zhao,Jiayi Zhang,Lijun Luo,Xiaohong Liu,Xia Li,Tianyan You,Chunjiang Zhao
Herein, a label-free, self-enhanced electrochemiluminescence (ECL) sensing strategy for divalent mercury (Hg(II)) detection was presented. First, a novel self-enhanced ECL luminophore was prepared by combining the ECL reagent tris(2, 2′-bipyridyl) dichlororuthenium(II) hexahydrate (Ru(bpy) 3 2+ ) and its co-reactant carbon nitride quantum dots (CNQDs) via electrostatic interactions. In contrast to traditional ECL systems where the emitter and its co-reactant underwent an intermolecular reaction, the self-enhanced ECL system exhibited a shortened electron-transfer distance and enhanced luminous efficiency because the electrons transferred from CNQDs to oxidized Ru(bpy) 3 2+ via an intramolecular pathway. Furthermore, the as-prepared self-enhanced ECL material was encapsulated in silica (SiO 2 ) nanoparticles to generate a [email protected] 2 luminophore. Based on the different affinity of [email protected] 2 nanoparticles for single-stranded DNA (ssDNA) and Hg(II)-triggered double-stranded DNA (dsDNA), a label-free ECL biosensor for Hg(II) detection was developed as follows: in the absence of Hg(II), ssDNA was adsorbed on [email protected] 2 surface via hydrogen bond, electrostatic, and hydrophobic interaction. Thus, quenched ECL signal was observed. On the contrary, in the presence of Hg(II), stable dsDNA was formed and carried the ssDNA separating from [email protected] 2 surface, resulting in most of [email protected] 2 existing in their free state. Therefore, a recovered ECL intensity was obtained. On this basis, Hg(II) was measured by the proposed method in the range of 0.1 nM-10 μM, with a detection limit of 33 pM. Finally, Hg(II) spiked in water samples was measured to evaluate the practicality of the fabricated biosensor.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）