Evidence-based Complementary and Alternative Medicine
·
2023
·
IF 2.65
Wei-Qi Yang,Zheng-Ming Qian,Meng-Qi Wu,Jian-Li Gao,Qi Huang,Yuan-Sheng Zou,Dan Tang
The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive compounds from PB is inadequate. In the current research, an online microextraction (OLME) coupled with a high-performance liquid chromatography coupled with the 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay (HPLC-ABTS) system for rapid analysis of antioxidants from PB was proposed. The PB sample (0.17 mg) was online extracted by mobile phase (acetonitrile and 0.2% acetic acid); a Poroshell 120 SB-Aq column was used for separation; then, an online ABTS assay system was used for screening the antioxidants. Finally, ten components were found in PB, and among them, eight components possessed antioxidant activities. Furthermore, five components (gallic acid, neochlorogenic acid, caffeic acid, chlorogenic acid, and an unknown compound) were proved as major antioxidants when compared with rutin as an antioxidant marker. The results showed that the developed OLME-HPLC-ABTS system was a simple, rapid, green, and efficient instrument for the screening of antioxidants from PB, which provides a powerful tool for the discovery of natural antioxidants in Chinese medicines.
研究领域:
生药学
·
分析化学
·
天然产物
·
中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Jinbin Lin,Yuye Hu,Junyang Xiao,Yixin Huang,Mengyun Wang,Haoyu Yang,Jing Zou,Baoling Yuan,Jun Ma
Fe(II)/peracetic acid (Fe(II)/PAA) process exhibits a great potential in abatement of micropollutants because of the fast reaction between Fe(II) and PAA. Similar to Fenton system, the slow reduction from Fe(III) to Fe(II) leads to the high dosage of Fe(II) in Fe(II)/PAA process. This study introduced 2,2′-azino-bis(3-ethylbenz- -othiazoline-6-sulfonate) (ABTS) as electron shuttle to improve the oxidation capacity of Fe(II)/PAA process by accelerating the redox cycle of Fe(III)/Fe(II). ABTS/Fe(II)/PAA process possessed high elimination efficiency of diclofenac in acidic conditions. Alcohols quenching experiments confirmed that ABTS •+ was the primary reactive species responsible for diclofenac elimination, although Fe IV O 2+ and carbon-centered radicals (R-O • ) also existed in ABTS/Fe(II)/PAA process. ABTS •+ was generated via various pathways, including the reactions of ABTS with Fe(III), Fe IV O 2+ and R-O • . ABTS was not an ideal electron shuttle because R-O • could decompose partial ABTS •+ to sulfoxides and sulfones. Two possible degradation pathways of diclofenac were proposed based on the detected transformation products. ABTS/Fe(II)/PAA process was highly-efficient for diclofenac elimination in underground water and reservoir water, while high concentration of humic acid had inhibition effect on diclofenac degradation. The present study provides a promising way to alleviate the intrinsic drawbacks of Fe(II)/PAA process and might advance its potential application in water treatment.
研究领域:
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yixin Huang,Jing Zou,Jiawen Li,Hongfang Ma,Siying Chen,Lingying Chen,Xiaobin Liao,Jun Ma
In this study, a rapid, sensitive and reliable spectrophotometric method for peroxydisulfate (PDS) determination in water was established. The proposed spectrophotometric method was based on the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) to form stable and green-colored ABTS radical (ABTS • + ) by ferrous ion (Fe(II))-catalyzed PDS. The formed ABTS • + performed well linear relationship with PDS concentration (R 2 > 0.999) in the range of 0–60 μM at four wavelengths of 415, 650, 732, and 820 nm under optimized conditions. The proposed Fe(II)-ABTS spectrophotometric method was highly sensitive with the absorptivity of 3.595 × 10 4 M −1 cm −1 , 1.530 × 10 4 M −1 cm −1 , 1.945 × 10 4 M −1 cm −1 and 1.768 × 10 4 M −1 cm −1 at four detection peaks of 415, 650, 732 and 820 nm, respectively. Additionally, the proposed Fe(II)-ABTS spectrophotometric method possessed well anti-interference capacity to common natural water constituents and was reliable to determine PDS concentration in water. What’s more, the proposed Fe(II)-ABTS spectrophotometric method was successfully applied in monitoring the change of PDS concentration during the degradation of diclofenac in heat-activated PDS process. In a word, the proposed Fe(II)-ABTS spectrophotometric method could be employed as a promising approach for the rapid and accurate measurement of PDS in water, and this study provided a new perspective for the measurement of PDS by the visible spectrophotometry .
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Colloid and Interface Science Communications
·
2023
·
IF 4.5
Chengyang Ban,Hanzhu Shi,Qiong Ding,Bin Wu,Ru Xia,Jiasheng Qian,Ming Cao
In this study, Fe/ABTS/PVP nanocomplexes (named FAPs) as photothermal-reinforced catalytic antibacterial agents were prepared using the coordination-induced assembly of Fe (III), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and polyvinylpyrrolidone (PVP). The redox pairs of Fe(II)/Fe(III) in FAPs can catalyze the H 2 O 2 to hydroxyl radicals (•OH) through Fenton reactions, thereby killing effect on bacteria. The photothermal effect of ABTS •+ in FAPs stimulated by near infrared (NIR) irradiation can further improve the catalytic effect, thereby generating more •OH and achieving better antibacterial activity. What's more, the reduction reaction between glutathione (GSH) and ABTS •+ results in the depletion of the GSH level, the redox pair of Fe(II)/Fe(III) is also beneficial in the consumption of GSH. This dual depletion of GSH further expands the intracellular oxidative stress. Thus, the integrated function of photothermal effect, catalytic property, and dual depletion of GSH by FAPs provides a new strategy for fighting against bacterial infections.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
抗菌研究
✓ 已验证
产品关联
DOI
高影响因子
BMC Complementary Medicine and Therapies
·
2020
·Vol. 20
·期 1
·1-10
·
IF 0
Li Hong-Ying,Fang Jiang-Ji,Shen Hua-Dan,Zhang Xue-Qiong,Ding Xiao-Ping,Liu Jun-Feng
Background Quantitation analysis and chromatographic fingerprint of multi-components are frequently used to evaluate quality of herbal medicines but fail to reveal activity of the components. It is necessary to develop a rational approach of chromatography coupled with activity detection for quality assessment of herbal medicines. Methods An on-line HPLC-ultraviolet detection-2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging (HPLC-UV-ABTS) method was developed to obtain the chromatographic fingerprints and ABTS +• inhibition profiles (active fingerprints) of Rehmanniae Radix (Dihuang) and Rehmannia Radix Praeparata (Shu Dihuang). Eighteen compounds showing ABTS +• inhibition activity were identified by HPLC-fourier-transform mass spectrometry (HPLC-FTMS). Verbascoside was used as a positive control to evaluate the total activities of the samples and the contribution rate of each compound. The similarities of the chromatographic and active fingerprints were estimated by the vectorial angle cosine method. Results The results showed that the HPLC-UV-ABTS method could efficiently detect antioxidant activity of the herbal medicine samples. The antioxidants were different between the two herbs and several new antioxidants were identified in Shu Dihuang. A function equation was generated in terms of the negative peak area (x) and the concentrations of verbascoside (y, μg/mL), y = 2E-07 × 4 - 8E-05 × 3 + 0.0079 × 2 + 0.5755x + 1.4754, R 2 = 1. Iridoid glycosides were identified as main antioxidants and showed their higher contributions to the total activity of the samples. The total contributions of the three main active components in the Dihuang and Shu Dihuang samples to the total activity, such as echinacoside, verbascoside and an unknown compound, were 39.2–58.1% and 55.9–69.4%, respectively. The potencies of the main active components in the Shu Dihuang samples were two to ten times those in the Dihuang samples. Similarity values for S12 in the chromatographic fingerprints and S03, S12 and P03 in the active fingerprints were less than 0.9. The three batches of samples might show their different quality with the other samples. Conclusions The results suggested that the combination of “quantity-effect” research strategy and the HPLC-UV-ABTS analysis method could comprehensively evaluate the active components and quality of Dihuang and Shu Dhuang.
研究领域:
中药学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
BIOMEDICAL CHROMATOGRAPHY
·
2024
·
IF 1.8
Yonglin Ma,Yue Zhang,Yu Zhao,Jiwen Wang,Qianqian Hu,Lianlin Yang,Shuzhen Chen,Yong Diao,Hongliang Ma
In this study, an online antioxidant assay based on HPLC-ABTS was mainly developed for screening the antioxidants of flavonoids from Astragali Radix (AR), and comparing the antioxidant capacity between traditional decoction pieces (TDP) of AR and cell wall-broken decoction pieces (CDP) of AR. The experimental results showed that the overall antioxidant capacity of CDP of AR was about twice as much as that of TDP of AR, which was specifically expressed as the antioxidant capacity of the screened antioxidants extracted from CDP was equivalent to 1.9–5.1 times that of those extracted from TDP, and three antioxidants were successfully screened, which were calycosin-7-O-β-D-glucoside, calycosin, and formononetin. The method established in this study is characterized by high efficiency and accuracy, which can simultaneously accomplish the screening of antioxidant components and the comparison of antioxidant capacity between samples, and provides a new method for the quality evaluation of AR from the perspective of antioxidant activity.
研究领域:
中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 5
·3873-3883
·
IF 2.431
Qian Zheng-ming,Huang Qi,Feng Xiao-long,Li De-qiang
Given the safety of synthetic antioxidants, people are increasingly inclined to develop and use natural antioxidants. However, rapid screening and identification of active ingredients from the complex systems of natural products is one of the major challenges for the modernization of traditional Chinese medicine. In this study, online extraction-high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry coupled with ABTS antioxidant assay (OLE-HPLC-DAD-QTOF-MS/MS-ABTS), in which sample preparation, separation, detection and antioxidant assay were integrated in a single step, was used to rapidly identify antioxidant components from the different parts of Citrus reticulata , including its peels, leaves, vascular bundies and seeds. After method validation (system adaptability, extraction yield and repeatability), the four samples of different botanical parts were analyzed. As a result, among 49 found chromatographic peaks, 33 peaks displayed varying degrees of antioxidant activity and 39 peaks were tentatively or unambiguously identified based on their retention time, UV spectrum and mass spectrometry information and the comparison with reference substance and data in literature, including 10 phenolic acids, 9 polymethoxy flavonoids, 7 flavonoid- O -glycosides and 13 other ingredients. It is concluded that the established method is simple, fast, and reliable, which not only saves the materials and solvents as well as time, but also reduces the risk of degradation of chemical components during the extraction process, suitable for rapid screening and identification of antioxidant components of complex mixture.
研究领域:
生药学
·
分析化学
·
天然产物
·
中药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2012
·
IF 4.555
Ruimin Li,Cen Xiong,Zhiyou Xiao,Liansheng Ling
A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ). Oligonucleotide 5′-GTGGGTAGGGCGGGTTGG-3′ (Oligo-1) formed G-quadruplex structure in the presence of K + , it acted as a horseradish peroxidase (HRP) mimicking DNAzyme when binding hemin and catalyzed the oxidation of colorless ABTS 2− to green ABTS − by H 2 O 2 , which was produced by the reaction of cholesterol and oxygen that catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the color change of ABTS 2− by combining these two reactions. Under the optimum conditions, the absorbance was proportional to the concentration of cholesterol over the range of 1.0–30 μM, with a linear regression equation of A = 0.362 + 0.0256 C ( C : μM, R = 0.998) and a detection limit of 0.10 μM (3 σ /slope). Moreover, the practicability of the assay in the detection of cholesterol in human serum was studied as well.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Ru-Jia Yu,Wei Ma,Mao-Pan Peng,Zhi-Shan Bai,Yi-Tao Long
A novel redox-mediated chromogenic reaction was demonstrated based on the reaction between HAuCl 4 and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), which generate various color responses from red to green in the resulting solutions. Various redox substance could be used to mediate the reaction and trigger a distinct color response. We established a sensitive hydrogen peroxide colorimetric sensor based on the redox-mediated chromogenic reaction and depicted the application both in detection of enzyme and in an immunoassay . Combining the traditional chromogenic reagent with gold nanoparticles , our assay has the advantage in short response time (within three minutes), high sensitivity (10 −12 g mL −1 for HBsAg) and stability.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Wei Yang,Ruicheng Xu,Feifei Mu,Jérome Chauvin,Serge Cosnier,Xue-Ji Zhang,Dan Shan
Uncontrolled diffusion and instability of redox mediators remain major challenges limiting the durability and fidelity of electrochemical biosensors. Here, we report a biomimetic strategy that encapsulates 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) within phospholipid vesicles to construct a vesicle-confined electron relay platform for hydrogen peroxide (H 2 O 2 ) sensing. The confined aqueous microenvironment preserves the redox activity of ABTS, prevents mediator leakage and degradation, and enables efficient electron shuttling with horseradish peroxidase (HRP). Consequently, the biosensor achieves ultrasensitive electrochemical detection (LOD: 2.53 nM), retains >70 % of its activity after 30 days at 4 °C, and endures over 500 CV cycles without signal loss. In parallel, a complementary chromogenic output provides an intuitive visual response in the micromolar range, underscoring the synergistic advantages of recyclable electrochemical relays versus one-off colorimetric products. Importantly, the platform enabled intracellular H 2 O 2 monitoring in living cells, validating its biocompatibility and robustness under biologically relevant conditions. This organelle-inspired, dual-mode sensing architecture offers a generalizable framework for stabilizing redox mediators through nanoconfinement and opens new avenues for durable, field-deployable point-of-care biosensing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Shen Peng,Yuqin Wang,Junjun Yang,Lei Wang
A new method for the quantification of trace periodate (PI) concentration was developed. The Br - -ABTS method was based on the oxidation coloration reaction. Under Br - catalysis, the PI rapidly oxidized ABTS to the green ABTS •+ , which could be accurately determined by UV-Vis spectrophotometer. The absorbance of ABTS •+ was remarkably correlated linearly with the concentration of PI at 415 nm, and the slope of standard curve was (3.77 ± 0.01)× 10 4 M −1 ·cm −1 . The proposed method had high sensitivity and the slope at different optical paths showed a good multiplicative relationship for concentration values as low as 0.02 μM to be measured. The existence of different solutes in river and lake water did not affect the accuracy of Br - -ABTS method, the generated ABTS •+ could maintain good stability within 30 min, and the recovery of PI was between 95%− 105%. The stoichiometric coefficient between PI and ABTS •+ was 1.12 at pH 3.0. In addition, a common reductant (hydroxylamine), transition metal ions and inorganic anions (Cl - , SO 4 2- and HCO 3 - ) did not affect the Br - -catalyzed oxidation of ABTS for the measurement of PI concentration. Consequently, Br - -ABTS is a feasible method to determine the concentration of PI.
✓ 已验证
产品关联
DOI
高影响因子
ACS ES&T Water
·
2025
·
IF 4.3
Shule Duan,Zhimin Qiang,Yu Lei,Shaogang Liu,Ting Xie,Zhenyu Wang,Huiyu Dong
Spectrophotometry can efficiently determine permanganate (PM)-based chemical oxygen demand (CODMn) in various waters, but existing methods face challenges, such as large reagent requirements and interference from complex water matrices. Here, we developed a novel spectrophotometric method for determining the CODMn using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as an indicator. The kinetics and thermodynamics of the PM + ABTS reaction were investigated, revealing a pseudo-first-order rate constant (k′) of 36.4–48.6 s–1 at [PM]0:[ABTS]0 molar ratios of 1:60–1:100 (pH 6.5 and 298 K) and an Arrhenius activation energy (Ea) of 17.5 kJ mol–1. Compared to the PM + DPD (N,N-diethyl-p-phenylenediamine) reaction, which has a k′ of 29.4–47.5 s–1 under identical conditions and an Ea of 47.4 kJ mol–1, the PM + ABTS reaction demonstrated greater adaptability for rapid CODMn determination. Furthermore, the ABTS method showed higher sensitivity and accuracy for CODMn determination than the DPD method, with a higher slope of the linear calibration curve (0.228 L mg–1 cm–1 vs 0.101 L mg–1 cm–1) and a lower limit of quantification (0.03 mg L–1 vs 0.05 mg L–1). In addition, both methods showed high robustness against different water matrices, including real waters. Although the ABTS method has a higher reagent cost than the DPD method, it offers sensitive, rapid, and accurate CODMn determination, making it suitable for time-sensitive water quality assessments.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）