Applied Chemistry for Engineering
·
2025
·Vol. 36
·期 1
·36-42
·
IF 0.6
Gyu Won Woo,Eun Kwang Lee
본 연구에서는 스크린 인쇄 기술을 활용하여 유연 기판인 polyimide (PI) 필름에 내부 이온 게이트 유기 전기 화학 트랜지스터(internal gated transistor, IGT)를 제작하였다. 기존의 복잡한 공정 없이 저비용으로 제작하였으며, 유기 전기 화학 트랜지스터(OECT)의 채널로 주로 사용되는 전도성 고분자인 poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS)와 D-소르비톨을 혼합하여 외부 전해질 없이 채널 내에서 자체적으로 이온 (de)doping 메커니즘을 구현하였다. 그 결과, 스크린 인쇄 IGT는 기존 OECT 대비 향상된 전도성과 성능 지표를 나타냈다. D-소르비톨 첨가 후 PEDOT:PSS의 시트 저항 값이 기존 대비 약 1/2로 감소하여 전도성이 향상되었고, 표면 결정성 또한 개선되었다. 전기적 성능 평가에서 IGT는 OECT 대비 출력 곡선 특성(VGS = 1.6 V)에서 약 4배 증가한 것으로 나타났으며, ION/IOFF 비율은 약 4.3배 높았다. 이동도 계산 결과 OECT는 0.6987 F V-1 s-1 cm-1, IGT는 0.9578 F V-1 s-1 cm-1로 IGT가 높은 값을 보였고, 시간 응답 속도 상수 τ는 τOECT = 0.50 s, τIGT = 0.34 s로 더 빠른 응답 속도를 나타내었다.
The device fabricated in this study was an internal ion-gated organic electrochemical transistors (IGTs) using screen printing technology on a flexible polyimide (PI) film substrate. This fabrication method is a cost-effective and simple method without existing deposition techniques or complicated processes. The conductive polymer, poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS), commonly used as a channel material in organic electrochemical transistors (OECTs), was blended with D-sorbitol to enable a self-ion (de)doping mechanism within the channel, eliminating the requirement for an external electrolyte. The screen-printed IGT exhibited enhanced conductivity and performance compared to conventional OECTs. Analysis of the physical and chemical properties of PEDOT:PSS with D-sorbitol revealed a significant reduction in sheet resistance. The surface analysis further confirmed an enhancement in crystallinity. Electrical performance evaluation involved a comparative study with conventional OECTs. The IGT device demonstrated a 4 times increase in output curve characteristics at VGS = 1.6 V compared to OECTs. Additionally, the ION/IOFF ratio was 4.3 times higher for the IGT. Calculations of µC* to assess device mobility indicated a notable improvement, with values of 0.9578 F V-1 s-1 cm-1 for IGT compared to 0.6987 F V-1 s-1 cm-1 for OECTs. In time constant analysis, τOECT = 0.50 s and τIGT = 0.34 s indicate that IGTs have a faster response time.
研究领域:
材料科学
·
电子工程
·
化学工程
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DOI
高影响因子
Biomolecules
·
2024
·Vol. 14
·期 5
·581
·
IF 4.8
Jiaming Cao,Boyuan Xiang,Baojie Dou,Jingfei Hu,Lei Zhang,Xinxin Kang,Mingsheng Lyu,Shujun Wang
Peptides possessing antihypertensive attributes via inhibiting the angiotensin-converting enzyme (ACE) were derived through the enzymatic degradation ofTrichiurus lepturus(ribbonfish) using alkaline protease. The resulting mixture underwent filtration using centrifugation, ultrafiltration tubes, and Sephadex G-25 gels. Peptides exhibiting ACE-inhibitory properties and DPPH free-radical-scavenging abilities were isolated and subsequently purified via LC/MS-MS, leading to the identification of over 100 peptide components. In silico screening yielded five ACE inhibitory peptides: FAGDDAPR, QGPIGPR, IFPRNPP, AGFAGDDAPR, and GPTGPAGPR. Among these, IFPRNPP and AGFAGDDAPR were found to be allergenic, while FAGDDAPRR, QGPIGPR, and GPTGPAGP showed good ACE-inhibitory effects. IC50values for the latter peptides were obtained from HUVEC cells: FAGDDAPRR (IC50= 262.98 μM), QGPIGPR (IC50= 81.09 μM), and GPTGPAGP (IC50= 168.11 μM). Peptide constituents derived from ribbonfish proteins effectively modulated ACE activity, thus underscoring their therapeutic potential. Molecular docking and modeling corroborated these findings, emphasizing the utility of functional foods as a promising avenue for the treatment and prevention of hypertension, with potential ancillary health benefits and applications as substitutes for synthetic drugs.
研究领域:
食品科学
·
生物化学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2270-2281
·
IF 3.289
Han Wu,Xin Rui,Wei Li,Yu Xiao,Jianzhong Zhou,Mingsheng Dong
This study explored a novel strategy to develop solid-state whole-grain oats as a novel carrier of lactic acid bacteria and a nutraceutical supplement rich in ACE inhibitory peptides. Oats were fermented by Lactobacillus plantarum B1-6, Rhizopus oryzae, or a combination of L. plantarum B1-6 and R. oryzae. L. plantarum showed a much better growth performance in oats when it was combined with R. oryzae than when it was cultured alone, as evidenced by an increase in viable cell count to 9.70 log cfu g−1 after 72 h of fermentation. The coinoculated fermented oats (CFO) and the R. oryzae-fermented oats (RFO) were then selected for investigations on protein hydrolysis and on the functional properties of the released bioactive peptides. The results showed that the soluble protein contents changed from 7.05 mg g−1 to 14.43 and 10.21 mg g−1 for CFO and RFO, respectively. However, the degree of hydrolysis and the content of peptides with molecular masses less than 10 000 Da indicated that the CFO proteins can be degraded to a greater degree. As analyzed by electrophoresis and reversed-phase high-performance liquid chromatography, the protein and peptide profiles of CFO and RFO demonstrated that the proteins from CFO were more obviously hydrolyzed and more small peptides were obtained. In addition, both CFO and RFO presented higher ACE inhibitory activities than unfermented oats, whereas the protein extracts from CFO exerted a lower IC50 value of 0.42 mg protein per mL compared with the protein extracts from the other samples. This research has broadened our knowledge on the development of whole-grain oat products as a probiotic carrier and on the difference between mixed solid-state fermentation (SSF) and fungi SSF in terms of protein degradation and the capacity to release ACE inhibitory peptides. Our approach could be used to obtain probiotic food products and probably to develop oats as a potential therapeutic ingredient targeting hypertension.
研究领域:
食品科学
·
微生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Siyuan Peng,QingPing Liang,Wenjing Qiao,Yangyang Han,Haijin Mou,Min Yang
Hypertension remains a major global health challenge and is associated with increased cardiovascular risk. Angiotensin-converting enzyme (ACE) plays a pivotal role in blood pressure regulation; however, conventional synthetic ACE inhibitors are often accompanied by undesirable side effects. Nattokinase (NK), a serine protease derived from natto, has shown antihypertensive potential via bioactive peptides released during digestion. In this study, we identified two novel ACE-inhibitory peptides (LGG and TW) from simulated digestion of NK. LGG exhibited potent competitive inhibition (IC 50 = 10.78 μM), while TW acted noncompetitively. Molecular docking and dynamics confirmed stable interactions, and network pharmacology suggested multitarget cardiovascular regulation. These findings support the potential of NK-derived peptides as functional food components for blood pressure management.
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Dankui Liao,Lei Wang,Qian Zhou,Lixia Sun,Jianhua Sun,Zhangfa Tong,Guangzhi Zhou
Food-derived angiotensin-converting enzyme (ACE)-inhibitory peptides (ACEIPs) with antioxidant and antibacterial properties can function as food additives to alleviate hypertension-induced oxidative stress and bacterial infections. This study used bioinformatics to analyze Gly-Xaa-Pro ACEIPs, identifying Gly-Pro-Pro (GPP) from buckwheat. GPP exhibited potent free-radical-scavenging capacity and at 4 mg/mL eradicated both E. coli and S. aureus (0 % survival vs 14.1 %/4.6 % with GRP; p < 0.001), demonstrating superior antioxidant and bactericidal potency. Multispectral techniques, thermodynamic analysis, and molecular docking simulations were used for a detailed molecular-level investigation to better understand the binding mechanism between GPP and ACE. The results indicated that GPP's binding to ACE is non-competitive and acts as an inhibitor. The inhibitory effect of GPP on ACE from its moderate binding ( K a = 1.525 × 10 4 M −1 ), which alters the microenvironment and conformation of ACE. The binding process is primarily driven by entropic forces arising from hydrophobic interactions and proceeds spontaneously due to ΔG<0. Through multi-molecular synergy (Hill coefficient n = 513), GPP forms hydrogen bonds with the Asp 121, Glu 123, Tyr 135, and Trp 220 residue sites outside ACE's active center, thereby inhibiting ACE activity. In conclusion, the research of GPP provides a theoretical basis for developing multifunctional blood-pressure-lowering dietary supplements.
研究领域:
食品科学
·
生物信息学
·
分子营养学
·
肽类生物化学
·
功能食品
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Heyong Wang,Meiluan Wang,Guixiang Huang,Mingye Zhao,Yongzhen Peng,Ning Zhu,Zheng Fang,Yujing Hu,Kai Guo
Optically active ethyl ( R )-2-hydroxy-4-phenylbutanoate (( R )-HPBE), is a key component in the production of angiotensin-converting enzyme (ACE) inhibitors, which are essential for treating hypertension. However, the high cost of starting materials has been a barrier to the industrial synthesis of ( R )-HPBE. Herein, we have established for the first time a four-step enzymatic reaction in continuous flow for the efficient synthesis of ( R )-HPBE from inexpensive benzaldehyde and pyruvic acid. This route also overcomes the issues of chemical disequilibrium and incompatibility of reaction conditions that are common in multi-enzyme cascades. The immobilization methods for aldolase/ene-reductase/keto-reductase were developed, and the immobilized enzymes were initially used in three individual flow modules to produce ( R )-2-hydroxy-4-phenylbutanoic acid (( R )-HPB), achieving high space–time yields of 3103 g (OPBEA) L −1 d −1 , 1685 g (OPBE) L −1 d −1 and 1709 g ( R )-HPB L −1 d −1 respectively. Moreover, integrating three modules in continuous flow achieved a space–time yield of about 350 g ( R )-HPB L −1 d −1 after 108 h, and maintained high operational stability with a conversion of >99 % and a product ee of >99.9 % after 192 h. Subsequently, the ethyl esterification of ( R )-HPB as the fourth module for producing ( R )-HPBE obtained a high space–time yield of 2117 g ( R )-HPBE L −1 d −1 , marking a substantial 74.5-fold increase over the batch system.
研究领域:
生物化学
·
制药工程
·
酶工程
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Renjie Zhou,Cheng Yang,Ting Xie,Jian Zhang,Chenqiang Wang,Ziqiang Ma,Lianfu Zhang
Amadori compounds (ACs) are the first stable product formed in the initial stage of the Maillard reaction and are widely present in processed foods. In addition to being widely known as the flavor precursors, ACs have attracted attention in recent years because many functions have been revealed, including ACE inhibition. In this work, we synthesized four ACs ( N -(1-Deoxy-D-fructos-1-yl)-glycine (Fru-Gly), N -(1-Deoxy-D-fructos-1-yl)-proline (Fru-Pro), N -(1-Deoxy-D-fructos-1-yl)-serine (Fru-Ser) and N -(1-Deoxy-D-fructos-1-yl)-threonine (Fru-Thr)) and characterized them using UPLC-Q-TOF-MS spectra. The four ACs’ angiotensin-converting enzyme (ACE) inhibitory activities were verified for the first time, the IC 50 value range was 1.447–4.204 mmol/L, the Fru-Gly has the best effect. And all the four ACs showed good digestion stability, the content remained above 70% after 5 h of continuous digestion simulation. In order to reveal the mechanism of ACs inhibiting ACE, we selected Fru-Gly, beacuse of the best inhibitory effect, to carried out enzyme kinetics, circular dichroism spectroscopy, fluorescence spectroscopy, thermodynamics and molecular docking analysis. The results showed that Fru-Gly was a noncompetitive inhibitor, it spontaneously binds to the inactive site of ACE by hydrogen bond and causes the conformation change of ACE to inactivate it. The reaction is easier to proceed at low temperatures.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Long Liu,Yanan Wei,Qing Chang,Huaju Sun,Kungang Chai,Zuqiang Huang,Zhenxia Zhao,Zhongxing Zhao
A novel, moderately hydrophilic peptide (RYL) with high ACE-inhibitory activity was screened ultrafast via a concept of waste conversion using waste. This novel peptide was screened from silkworm pupa using an Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic properties and specific selection for peptides due to Fe’s dual functions. The selected RYL, which has moderate hydrophilicity (LogP = −0.22), exhibited a comparatively high ACE-inhibitory activity (IC50 = 3.31 ± 0.11 μM). The inhibitory kinetics and docking-simulation results show that, as a competitive ACE inhibitor, RYL formed five hydrogen bonds with the ACE residues in the S1 and S2 pockets. In this work, both the screening carbon material and the selected ACE-inhibitory peptide were derived from agricultural waste (silkworm excrement and pupa), which offers a new way of thinking about the development of advanced uses of the silkworm byproducts and wastes.
研究领域:
生物技术
·
环境科学
·
药学
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产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2025
·
IF 1.3
Zhu Xianhong,Zhang Min,Qin Kunming,Qin Xueying,Chi Miaomiao,Zhang Yan,Wang Hongsen,Song Xichen,Gao Xun
Choerospondias axillaris (C. axillaris) , recognized as an effective herbal remedy for coronary heart disease (CHD), has been clinically utilized. Although the angiotensin-converting enzyme (ACE) has been extensively investigated as a pertinent target for CHD treatment, there is currently limited research on screening potential ACE inhibitors from C. axillaris . To advance the broader utilization of C. axillaris in coronary heart disease treatment, the target active components of ACE in C. axillaris were analyzed through the integration of magnetic-targeted fishing technology with functionalized magnetic nanospheres. Magnetic nanospheres functionalized with GO@Fe 3 O 4 @SiO 2 -ACE (SMGO-ACE) were characterized using a vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscope (TEM). The specific binding of lisinopril with the angiotensin-converting enzyme was employed to optimize the performance of the synthesized material and the relevant conditions during targeted fishing. Subsequently, five active ingredients were identified as (2R,3S)-Dihydrodehydroconiferyl alcohol, isovanillin, quinic acid, chrysin, and isorhamnetin. This study provides a precedent for the targeted extraction and separation of active ingredients in complex mixtures.
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DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1209
·
IF 5.1
Kanzhen Liu,Cuiping Pang,Qinghua Li,Jianghua Li,Guocheng Du,Guoqiang Zhang
Bioactive components of abalone and other marine organisms have attracted significant attention owing to their functional performance. The development of peptides with bioactivity like angiotensin-converting enzyme inhibitory (ACEi) and antioxidant properties is of great significance for chronic disease management and drug discovery. In this study, according to the issues of low utilization rate and bioactive content from the hydrolysate of abalone, single-factor and orthogonal experiments were designed to improve the utilization rate of abalone protein, and step hydrolysis with specific proteases was used to improve the overall biological activity of the hydrolysate. A total of 1937 peptide sequences were obtained from the highly bioactive components after separation and peptidomic analysis. Through virtual screening and molecular docking, 14 peptides exhibiting ACEi activity were identified and synthesized for experimental verification, with IC 50 values ranging from 0.05 to 0.54 mg/mL. Notably, nine of these peptides were powerful antioxidants. The developed step enzymatic hydrolysis and in silico screening-assisted preparation also provided a feasible and efficient method for exploring more bioactive peptides from diverse biomasses.
研究领域:
海洋生物学
·
药物化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Peng Zhang,Chang Chang,Haijie Liu,Qiaojuan Yan,Zhengqiang Jiang
Three protein-rich materials were pretreated with a self-designed alkaline electrolyzed water (AEW) pretreatment equipment to promote the release of angiotensin I-converting enzyme (ACE) inhibitory peptides by improving hydrolysis efficiency. According to degree of hydrolysis (DH) and ACE inhibitory activity, defatted walnut meal (DWM) was selected to study antihypertensive activity by hydrolysis in assistance of AEW pretreatment. It was found DWM pretreated with AEW at pH 11.5 followed by Alcalase hydrolysis exhibited the highest DH (21.96%), protein recovery rate (94.76%), and ACE inhibitory activity (IC 50 of 0.137 mg/mL), in which AEW pretreatment resulted in the transformation of α-helix and β-turn to random coil and the destruction of hydrogen bonds to expose more cleavage sites to enhance hydrolysis efficiency. Thereafter, ACE inhibitory peptides GIENYRVV (IC 50 of 0.102 mg/mL) and AGRISTVNSHTLPVL (IC 50 of 0.20 mg/mL) were identified from the hydrolysate. Overall, AEW pretreatment was an innovative strategy to promote the release of antihypertensive peptides during hydrolysis.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 15
·期 1
·84
·
IF 5.1
Wanyu Yang,Xinyu Zou,Tianrong Zhang,Qingqing Liu,Ziyan Liu,Fan Li,Yuhong Luo,Yiwen Wang,Zhijun Qiu,Bin Zhang
This study aimed to identify novel angiotensin-converting enzyme (ACE)-inhibitory peptides from royal jelly proteins (RJPs) by integratingin silicodigestion, virtual screening, andin vitroevaluation. Three major royal jelly proteins (MRJP1-3) were subjected toin silicodigestion using 16 enzymatic systems, yielding 1411 unique peptides. Virtual screening based on predicted bioactivity, toxicity, water solubility, and ADMET profiles resulted in the selection of 27 candidate peptides. Molecular docking revealed strong binding affinities for these peptides compared with the positive control captopril, among which PYPDWSFAK and RPYPDWSF exhibited potent ACE-inhibitory activity, with IC50values of 110 ± 1.02 μmol/L and 204 ± 0.61 μmol/L, respectively. Kinetic analysis indicated that PYPDWSFAK acts as a mixed-type ACE inhibitor. Docking visualization demonstrated that PYPDWSFAK forms multiple hydrogen bonds with key residues in the ACE active pocket and directly coordinates with the catalytic Zn2+ion. Cellular assays showed that PYPDWSFAK was non-cytotoxic, suppressed Ang II–induced endothelial cell migration, restored NO and ET-1 balance, and enhanced SOD and GSH-Px activities. Overall, this study enriches the repertoire of ACE-inhibitory peptides derived from royal jelly proteins. Furthermore, PYPDWSFAK is identified as a promising ACE-inhibitory peptide with potential for incorporation into natural antihypertensive ingredients or functional foods.
研究领域:
食品科学与技术
·
生物信息学
·
分子生物学
·
心血管药理学
·
功能食品
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）