Aging and Disease
·
2024
·
IF 7
Jiamei Han,Judun Zheng,Qian Li,Huanle Hong,Jing Yao,Jiao Wang,Robert Chunhua Zhao
Cellular senescence is an irreversible and multifaceted process inducing tissue dysfunction and organismal aging, and thus the clearance of senescent cells can prevent or delay the onset of aging-related pathologies. Herein, we developed an augmented photothermal therapy strategy integrated with an antibody against β2-microglobulin (aB2MG) and an immune adjuvant imiquimod (R837) to effectively accelerate senescent cell apoptosis and clearance under a near-infrared light. With this strategy, the designed CroR@aB2MG enables the targeting of senescent cells and the application of photothermal therapy concomitantly, the initiation of immune clearance subsequently, and finally the realization of protective effects against senescence. Our results showed that the photo-induced heating effect caused senescent cells to quickly undergo apoptosis and the synchronous immune response accelerated the clearance of senescent cells in vitro and in vivo . Therefore, this photoactivated speedy clearing strategy may provide an efficient way for the treatment of senescence-related diseases by eliminating senescent cells with biomaterials.
研究领域:
生物医学
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免疫学
·
老年医学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 18
·10188
·
IF 4.9
Peng Zhao,Zifu Wang,Shimei Liao,Yangxin Liao,Shijun Hu,Jianchun Qin,Donghua Zhang,Xiaohui Yan
Salvia leucanthais a perennial herb of the genusSalviain the family Labiatae, which has a wide range of biological activities, mainly including inhibition of acetylcholinesterase, antibacterial, and anti-inflammatory activity. To explore the protective effects and mechanism of action ofS. leucanthaon Alzheimer’s disease (AD), the anti-AD activity of SLE (extracts ofS. leucantha) was determined by using a transgenicCaenorhabditis elegans(C. elegans) model (CL4176). Analyses included paralysis assay, phenotypic experiments, transcriptome sequencing, RNA interference (RNAi), heat shock assays, and gas chromatography-mass spectrometry (GC-MS). SLPE (S. leucanthapetroleum ether extract) could significantly delay CL4176 paralysis and extend the longevity ofC. elegansN2 without harmful effects. A total of 927 genes were significantly changed by SLPE treatment inC. elegans,mainly involving longevity regulatory pathways—nematodes, drug metabolism—cytochrome P450, and glutathione metabolic pathways. RNAi showed that SLPE exerted its anti-AD activity through up-regulation of the genegst-5; the most abundant compound in SLPE analyzed by GC-MS was 2,4-Di-tert-butylphenol (2,4-DTBP), and the compound delayed nematode paralysis. The present study suggests that active components inS. leucanthamay serve as new-type anti-AD candidates and provide some insights into their biological functions.
研究领域:
药理学
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神经科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Aging-US
·
2020
·
IF 4.831
Chen Diling,Qi Longkai,Guo Yinrui,Liu Yadi,Tang Xiaocui,Zhu Xiangxiang,Zeng Miao,Li Ran,Shuai Ou,Wang Dongdong,Xie Yizhen,Yuan Xujiang,Burton B. Yang,Wu Qingping
Our pre-experiments found that the brain circRNA sequence profiles and gut microbiota in AD-like mice were changed, as circNF1-419 could enhance autophagy to ameliorate senile dementia in AD-like mice, so we conclude that there might some connections between circRNA and gut microbiome. Therefore, we use the over-expressed circNF1-419 adeno-associated virus (AAV) animal system with the aim of identifying possible connections. Our results showed that over-expression of circ NF1-419 in brain not only influenced the cholinergic system of brain, but also changed the gut microbiota composition as the Candidatus Arthromitus , Lachnospiraceae FCS020 group , Lachnospiraceae UCG-006 , and [Eubacterium] xylanophilum group , and the intestinal homeostasis and physiology, and even the gut microbiota trajectory in new born mice. These findings demonstrate a link between circRNA and gut microbiome, enlarge the ‘microbiome- transcriptome’ linkage library and provide more information on gut-brain axis.
研究领域:
神经科学
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微生物学
·
分子生物学
·
胃肠病学
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DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2019
·
IF 0
Cai-Hong Li,Jun-Li Yang
This study is to investigate the potential of wolfberry water extracts for the treatment of Alzheimer’s disease (AD) and the relationship between their anti-AD property and the geographic locations in China. Total extracts of all wolfberry samples showed the potent to decrease self-induced A β 1-42 aggregation and Cu 2+ -induced A β 1-42 aggregation and the antioxidant capacity as well as the bioactivity for binding bio-metal. A transgenic drosophila model assay revealed that TE of S1 and S6 (S1-TE and S6-TE) at 100 μg/mL demonstrated the potent rescue effect on memory loss compared to the positive control memantine at 20 μg/mL. Further study also revealed the polysaccharide extracts could contribute to their anti-AD property, and the anti-AD activity conspicuously depends on growing environment. Wolfberry samples S1 and S6 showed much more potent anti-AD property. Meanwhile this study demonstrated that the Chinese commonly consumed wolfberry in boiling water might have the beneficial function to prevent AD pathology.
研究领域:
神经科学
·
植物化学
·
药理学
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产品关联
DOI
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Zhiming Wang,Yuedong He,Lijing Liao,Yuhao Zhang,Yucheng Zhao,Yibei Xiao,Xuefei Jiang,Fei Qiao
A glycosyl transferase , isolated from Angelica decursiva a medical herb rich in coumarin , shows C-glycosyl transferase activity by in vitro activity assay using 5,7-dihydroxycoumarin as substrate, producing a C-glycosylated product at position C′8 along with the main product at C′6 position. Catalytic promiscuity assay shows that Ad CGT also displays O- or C-glycosylation activity to other coumarins and flavonoids . When phloretin and 2,4,6-trihydroxyacetophenone were fed as substrates, Ad CGT catalyzed the formation of di-C-glycosides. Therefore, Ad CGT is a multifunctional glycosyltransferase with a broad substrate acceptability. This work highlights the potential of Ad CGT as a catalyst for glycosylation of coumarin and reveals a new regio-selective C-glycosyltransferase, providing a basis for exploring the mechanism of coumarin glycosylation.
研究领域:
生物化学
·
生物催化
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药物化学
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DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Junjie Yu,Dejin Ma,Shutong Li,Suyu Yang,Tianhua Li,Liang Kong,Yang Liu,Juan Zang,Yang Yu,Xuetao Li
Oxidative stress is considered a critical factor in the pathogenesis of Alzheimer's disease (AD). The interaction between oxidative stress and neuroinflammation is closely associated with the production of beta-amyloid (Aβ). Although many drugs exhibit potent anti-inflammatory and antioxidant properties, their poor water solubility and limited ability to penetrate the blood-brain barrier (BBB) often result in reduced efficacy, making accurate AD treatment challenging. Previous studies have shown that borneol (Bor) can enhance BBB permeability, while the MG1 peptide can specifically target M1-type microglial cells. In this study, we constructed reactive oxygen species (ROS)-responsive cascade-targeting liposomes (ROS@Res-Lip) using DSPE-PEG 2000 -MG1 and DSPE-TK-PEG 2000 -Bor co-modification. This design aimed to reduce ROS accumulation and enhance BBB penetration by incorporating ROS-responsive thioketal (TK) bonds and Bor, while leveraging the MG1 peptide to improve the targeting of resveratrol (Res) to M1-type microglia. As a result, the drug concentration in the brain was significantly increased, thereby improving therapeutic efficacy. Our findings demonstrate that ROS@Res-Lip can achieve anti-inflammatory, antioxidant, and neuroprotective effects by targeting M1-type microglia and promoting the conversion of M1-type microglia to M2-type microglia.
研究领域:
神经科学
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药剂学
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纳米技术
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生物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122219
·
IF 7.5
Qian Zhang,Fu Wei,Shumin Qin,Bixiang Nie,Lin Deng,Zheng Chen,Siyu Hua
Heterotrophic nitrifying-aerobic denitrifying (HN-AD) bacteria- Chlorella symbiotic systems face several limitations, including high energy consumption, weak symbiosis and low pollutant removal efficiency. To address these issues, this study optimized the aeration rate. In addition, three quorum sensing (QS) molecules, namely C4-HSL, C6-HSL and C8-HSL, were introduced based on the structural chain length gradient of signaling molecules. This approach aimed to evaluate their effects on the system's macroscopic performance and to explore the underlying enhancement mechanisms. The results showed that under the optimal aeration rate of 0.6 L·min −1 , C8-HSL significantly enhanced pollutant removal, increasing the average removal efficiencies of NH₄⁺-N, TN, TP and COD by 9.09%, 10.81%, 13.78% and 14.25%, respectively. Highthroughput sequencing revealed that C8-HSL markedly enriched Corynebacterium (48.72%), strengthening bacterial-algal symbiosis and improving nitrogen and phosphorus removal. PICRUSt2 functional prediction indicated that C8-HSL enhanced nitrogen metabolic flux by upregulating key genes involved in denitrification and nitrogen fixation ( nirK and nifDHK ), while also significantly increasing the abundance of the phosphorus metabolism related pat gene. qPCR analysis further confirmed the elevated expression of nirK and nifDHK , validating the reliability of PICRUSt2 predictions. These findings demonstrate that C8-HSL effectively improves the performance of the HN-AD bacteria- Chlorella symbiotic system and provides a theoretical basis for the engineering application of QS molecules.
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产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 19
·9856-9867
·
IF 6.317
Shuainan Zheng,Long Pan,Jianguang Hou,Aimei Liao,Yinchen Hou,Guanghai Yu,Xiaoxiao Li,Yongjian Yuan,Yuqi Dong,Penghui Zhao,Jie Zhang,Zheyuan Hu,Ming Hui,Jian Cao,Ji-Hong Huang
Neuroinflammation and intestinal microbiota cause pathological progression of Alzheimer's disease (AD), leading to neurodegeneration and cognitive decline. This study investigates the effects of wheat embryo globulin nutrient (WEGN) on depression, neuroinflammation, and intestinal microbial disorder caused by AD and its protective mechanism on cognitive impairment. Results demonstrated that rats in the WEGN group have lower feed intake but higher body weight than those in the control group. Notably, rats in the WEGN group have a higher number of cross grids and uprights and a smaller amount of fecal particles than those in the control group. Biochemical examinations revealed that rats in the WEGN group had lower expression of interleukin-1β, interleukin-6, and tumor necrosis factor α in hippocampus tissue and the expression of genes and proteins related to the TLR4/MyD88/NF-κB signaling pathway in AD rats was down-regulated compared to those in the control group. The 16S rRNA gene sequencing results demonstrated that WEGN treatment inhibits the increase of Erysipelotrichaceae, Erysipelatoclostridium, Erysipelotrichaceae, Corynebacterium, and Frisingicoccus, and the reduction of Lactobacillus in AD rats. WEGN has potential value as a practical food in alleviating neuroinflammation-related diseases such as AD.
研究领域:
神经科学
·
营养学
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微生物学
·
病理学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Sheng Li,Sidra Subhan,Liqin Zhou,Jing Li,Zhongxing Zhao,Zhenxia Zhao
Improving the adsorption capacity of the volatile organic compounds (VOCs) will inevitably decrease the desorption efficiency and hence increase more energy-consumption, which has become a crucial challenging problem for MOFs modification and composition toward VOCs adsorption. Herein, a thermal-conductive composition strategy was proposed to anchor BN nanosheets (BNNS) into HKUST-1 crystals via “N/O-Cu-O” interface. The formed HKUST-1@BNNS composite maintained high surface area (1633.7 m 2 /g having more microporous) and enhanced heat diffusivity (1.8 times of HKUST-1), simultaneously. This enabled significant increase of toluene adsorption capacity on HKUST-1@BNNS up to 4.3 mmol/g (2.8 times of HKUST-1) at ultra-low pressure ( P / P 0 ≈ 0.001) and higher desorption rate of toluene ( k ds = 29.3 × 10 −2 , 4.0 times of HKUST-1) with lower desorption activation energy ( E d = 33.5 kJ/mol). Compared with many other state-of-the-art adsorbents (e.g., MOFs, zeolites and activated carbons), it has a much higher adsorption capacity and regeneration rate. Thus, thermal-conductive MOFs construction can be deemed as smart strategy to enhance adsorption efficiency and save desorption energy for VOCs capture.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2023
·
IF 5.5
Jing-Jing Xu,Jiao Luo,Heng Xi,Jin-Bu Xu,Lin-Xi Wan
A series of novel N -aryl-debenzeyldonepezil derivatives ( 1–26 ) were designed and synthesized as cholinesterase inhibitors by the modification of anti-Alzheimer’s disease drug donepezil, using Palladium catalyzed Buchwald-Hartwig cross-coupling reaction as a key chemical synthesis strategy. In vitro cholinesterase inhibition studies demonstrated that the majority of synthesized compounds exhibited high selective inhibition of AChE. Among them, analogue 13 possessing a quinoline functional group showed the most potent AChE inhibition effect and significant neuroprotective effect against H 2 O 2 -induced injury in SH-SY5Y cells. Furthermore, Compound 13 did not show significant cytotoxicity on SH-SY5Y. These results suggest that 13 is a potential multifunctional active molecule for treating Alzheimer’s disease.
研究领域:
药物化学
·
神经生物学
·
老年医学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shumin Qin,Bixiang Nie,Fu Wei,Yan Lin,Lang Jiang,Qian Zhang
The symbiotic system of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria and Chlorella shows significant nitrogen and phosphorus removal in biogas slurry treatment. It uses quorum sensing signaling molecules to form symbiotic biofilms, significantly boosting nitrogen and phosphorus removal efficiency. This study systematically explored the effect of exogenous N-octanoyl-DL-homoserine lactone (C8-HSL) concentrations on pollutant removal efficiency, extracellular polymeric substance (EPS) production, and microbial dynamics in the HN-AD bacteria- Chlorella symbiotic system. Results showed that the pollutant removal of the symbiotic system is maximized when the C8-HSL concentration is 0.8 μM. At concentrations below 0.8 μM, the system significantly enhances pollutant removal through increased EPS secretion. However, when the concentration exceeds 0.8 μM, both EPS production and pollutant removal are inhibited. Metagenomic analysis shows that C8-HSL at 0.8 μM can enrich key bacterial genera such as Halomonas and Sphingobium . By regulating key genes like narGHI , nirKS , phnX , and ppa , it adjusts their metabolic pathways, thereby influencing nitrogen and phosphorus removal. This highlights the crucial role of C8-HSL concentration in pollutant removal within HN-AD- Chlorella symbiotic systems.
研究领域:
环境微生物学
·
水处理技术
·
生物修复
·
分子生态学
·
环境工程
·
微藻生物技术
·
信号分子调控
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产品关联
DOI
高影响因子
Natural Products and Bioprospecting
·
2025
·Vol. 15
·期 1
·1-15
·
IF 4.9
Huang Wen,Liu Yihan,Jiang Haixin,Guo Dongxue,Song Yi,Wang Junqi,Li Luqi,Zhang Qiang
Bioactive compounds from food-compatible medicinal herbs have shown promise as preventive agents against age-related neurodegenerative conditions, particularly Alzheimer’s disease (AD). The present work aimed to find Lobetyolin as a new suppressor of Aβ aggregation and its interventions on abnormal metabolism in AD. Aβ-expressing Caenorhabditis elegans (strain CL4176) and wild-type worms were employed to evaluate paralysis onset, lifespan, cerebral Aβ deposition, and intracellular reactive oxygen species (ROS) after Lobetyolin administration. Untargeted ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) metabolomics coupled with RNA-seq transcriptomics was carried out to profile systemic metabolic and gene-expression changes. Differential metabolites and transcripts were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and pathway-impact analyses; hub targets were prioritized by integrating enrichment scores with in-silico docking. Lobetyolin (12.5–50 µM) markedly protected C. elegans from Aβ-driven toxicity and oxidative stress. In CL2006 worms, β-amyloid deposits fell by 54.8 ± 9.4%, while paralysis in CL4176 was delayed by 20.9 ± 4.5%. Lifespan increased by up to 18.2% in CL4176 and 25.0% in wild-type N2 worms. Concomitantly, intracellular ROS declined maximally by 28.1 ± 8.9% (N2) and 22.4 ± 3.8% (CL4176). Integrative metabolomic–transcriptomic analyses, validated by RT-qPCR, revealed selective remodeling of glutathione metabolism: gst-38 expression was suppressed, whereas gst-1 was elevated. Lobetyolin confers neuroprotective and geroprotective benefits in vivo , primarily through reprogramming glutathione-centered redox metabolism and selectively modulating glutathione-S-transferases (GST) isoforms. These findings position Lobetyolin as a promising dietary lead compound for AD prevention and healthy aging interventions. Graphical
研究领域:
神经科学
·
药理学
·
代谢组学
·
转录组学
·
衰老研究
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）