Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Xiaodong Zhu,Yuxue Dai,Yuanling Sun,Hao Liu,Weiyan Sun,Yanna Lin,Dandan Gao,Rui Han,Xueying Wang,Chuannan Luo
The concentration of alpha-fetoprotein (AFP) rises greatly in patients with liver cancer and it is a challenge to construct a sensitive AFP detection method with wide range. Therefore, an easy and label-free sensing electrochemical platform for AFP detection with wide concentration range had been designed in this work. Firstly, MnO 2 functionalized mesoporous carbon hollow sphere (MCHS@MnO 2 ) with optimal performance was synthesized by regulating experimental conditions and characterized by scanning electron microscope (SEM), high resolution transmission electron microscopy (HRTEM), x-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), etc. Then, it was immobilized on glassy carbon electrode (MCHS@MnO 2 /GCE) to build an immunosensor for the detection of AFP. The MCHS@MnO 2 /GCE can catalyze decomposition of H 2 O 2 to generate electrochemical signal, and the signal will decrease after capturing AFP. Due to good electrocatalytic activity of MCHS@MnO 2 to H 2 O 2 , the immunosensor achieved indirect detection of AFP with wide sensing range from 0.10 ng mL −1 to 420 ng mL −1 and a detection limit of 0.03 ng mL −1 . Furthermore, the method had been proven to be satisfactory selectivity and reproducibility, and it was successfully applied to determine the content of AFP in human serum samples with satisfactory results. This method is expected to be used for early diagnosis and prognosis examination of liver cancer patients.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
·
2021
·Vol. 94
·期 3
·860-868
·
IF 5.488
He Yuhan,Li Lijun,Li Xiaoxuan,Lin Chubing,Zhang Yan
According to its characteristics, borate can recognize and adsorb target glycoproteins and specific binding properties of antigens and antibodies. This article introduces a preparation method for a magnetic immune SERS sensor, and it is used for the specific identification and detection of trace AFP in human serum. The test used AFP as the target protein and 4-mercaptophenylboronic acid (4-MPBA) modified gold-coated silver nanoparticles (Ag@AuNPs) as the signal factor. After the AFP antibody was grafted on the boric acid group, the SERS detection probe (Ag@Au@4-MPBA@Anti-AFP) was obtained. On the surface of boric acid-functionalized (-P) magnetic silica particles, AFP antibody was modified, and the particles were used as magnetic immunocapture probes (Fe3O4@SiO2-P@Anti-AFP). Finally, the sandwich structure complex of “detection probe-target protein-capture probe” was successfully constructed. The prepared magnetic immune SERS sensor exhibits excellent selectivity and high sensitivity for AFP detection; based on the signal-to-noise ratio S/N = 3, the detection limit can reach 1.0 ng/mL; by performing a spike test on AFP in human serum, the sensor can be used to detect AFP in actual samples. The recovery rate was between 85.8% and 105.7%, and the RSD distribution was ≤3.52%. The sensor was easy to prepare, required no complex processing of samples and can achieve rapid detection, so that it is expected to become a new strategy for clinical detection of AFP.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Lei Ji,Zhankui Guo,Tao Yan,Hongmin Ma,Bin Du,Yueyun Li,Qin Wei
An ultrasensitive sandwich-type electrochemical immunosensor for quantitative detection of alpha fetoprotein (AFP) was proposed based on a novel signal amplification strategy in this work. Carbon decorated Fe 3 O 4 magnetic microspheres (Fe 3 O 4 @C) with large specific surface area and good adsorption property were used as labels to anchor palladium nanoparticles (Pd NPs) and the secondary antibodies (Ab 2 ). Pd NPs were loaded on Fe 3 O 4 @C to obtain Fe 3 O 4 @C@Pd with core–shell structure by electrostatic attraction , which were further used to immobilize Ab 2 due to the bonding of Pd-NH 2 . A signal amplification strategy was the noble metal nanoparticles , such as Pd NPs, exhibiting high electrocatalytic activities toward hydrogen peroxide (H 2 O 2 ) reduction. This signal amplification was novel not only because of the great capacity, but also the ease of magnetic separation from the sample solution based on their magnetic property. Moreover, carboxyl-functionalized multi-walled carbon nanotubes (MWCNTs-COOH) were used for the immobilization of primary antibodies (Ab 1 ). Therefore, high sensitivity could be realized by the designed immunosensor based on this novel signal amplification strategy. Under optimal conditions, the immunosensor exhibited a wide linear range of 0.5 pg/mL to 10 ng/mL toward AFP with a detection limit of 0.16 pg/mL ( S / N =3). Moreover, it revealed good selectivity, acceptable reproducibility and stability, indicating a potential application in clinical monitoring of tumor biomarkers.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
ChemElectroChem
·
2013
·Vol. 1
·期 2
·441-447
·
IF 0
Qunfang Li,Dianping Tang,Fangming Lou,Ximei Yang,Guonan Chen
Graphical Multitasker: A sensitive and feasible multiplexed immunoassay protocol for simultaneous electrochemical determination of biomarkers is presented. The assay uses multifunctionalized graphene nanotags on a cyclodextrin-modified immunosensor. A sensitive and feasible multiplexed immunoassay protocol for simultaneous electrochemical determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was developed using multifunctionalized graphene nanotags on a cyclodextrin-modified immunosensor. The sensor was fabricated via the guest–host chemistry between the immobilized cyclodextrin and anti-CEA/anti-AFP antibodies. Two nanotags including cyclodextrin-thionine-graphene and cyclodextrin-ferrocene-graphene nanostructures were synthesized based on a wet chemistry method. The synthesized nanotags were employed for the labelling of horseradish peroxidase (HRP)–anti-AFP and HRP–anti-CEA conjugates, respectively. With a sandwich-type assay format, the signal was monitored through the labelling of HRP toward catalytic reduction of H 2 O 2 at various peak potentials in the presence of thionine and ferrocene, respectively. Under optimal conditions, the multiplexed immunoassay enabled the simultaneous determination of CEA and AFP with wide working ranges of 0.001–60 ng mL −1 for AFP and 0.003–40 ng mL −1 for CEA. The detection limits were 0.5 pg ml −1 for AFP and 0.8 pg ml −1 for CEA (at 3 s B ). No obvious nonspecific adsorption and cross-talk were observed during a series of analyses to detect target analytes. Intra- and inter-assay coefficients of variation (CV) were below 9.5 %. Importantly, the methodology was also evaluated for the analysis of AFP and CEA in the clinical human serum specimens, receiving a good relationship between the developed immunoassay and a commercialized electrochemiluminescence enzyme-linked immunoassay.
研究领域:
生物医学工程
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiayi Wu,Lujie Wang,Hongxin Wang,Xinhui Gu,Yucheng Zhou,Fengna Xi
Sensitive detection of alpha-fetoprotein (AFP) is crucial for early liver cancer screening. In this work, an immunosensor is constructed by combining the immunorecognition interface and enhanced electrochemiluminescence (ECL) of luminol under neutral condition utilizing cobalt oxide (Co 3 O 4 ) nanozymes confined in nanochannels, which realizes highly sensitive detection of AFP. Indium tin oxide (ITO) conductive glass is used as the supporting electrode, and silica nanochannel film (SNF) is simply grown on the ITO surface using the Stöber method. Co 3 O 4 nanozymes are in situ grown within the SNF nanochannels by a one-step electrodeposition method. The rigid 2D nanochannel array of SNF effectively confines the Co 3 O 4 nanozyme, significantly enhancing its stability. Epoxy modification on the external surface of SNF enables covalent attachment of AFP antibodies, leading to recognition interface. Confined Co 3 O 4 exhibits peroxidase-like activity, catalyzing the generation of reactive oxygen species (ROS) from hydrogen peroxide (H 2 O 2 ), enhancing luminol oxidation and boosting the ECL signal under neutral conditions. Upon AFP binding, antibodies capture AFP to form immunocomplexes, causing reduced diffusion of luminol and H 2 O 2 to the electrode surface, decreasing the ECL signal. The constructed immunosensor exhibits a linear range for AFP detection from 100 fg/mL to 10 ng/mL, with a limit of detection (LOD) of 18.5 fg/mL. AFP detection in serum samples from liver cancer patients or healthy individuals is successfully achieved.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Min Cui,Zhiling Song,Yumin Wu,Bing Guo,Xiaojian Fan,Xiliang Luo
Biocompatible polymers, such as poly(ethylene glycol) (PEG), are of great significance in the development of bio-interfaces and biosensors, as they possess excellent biocompatibility and are easy for modification. A novel highly biocompatible polymer composite was synthesized herein through electrochemical polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a PEG derivative, 4-arm PEG terminated with thiol groups . The electrodeposited conducting polymer composite of PEG doped PEDOT (PEDOT/PEG) exhibited flake-like nanostructure , large surface area and outstanding stability. In order to further immobilize antibodies, gold nanoparticles (AuNPs) were introduced to the PEDOT/PEG composite surface through their unique interaction with the thiol groups . AuNPs modified PEDOT/PEG provided a desirable support for the immobilization of various biomolecules such as antibodies for alpha fetoprotein (AFP), a vital tumor biomarker for liver cancer. The fabricated AFP biosensor demonstrated favorable selectivity, high sensitivity, and ultralow detection limit. Furthermore, owing to the presence of PEG polymers that are highly hydrophilic , such AuNPs/PEDOT/PEG based AFP biosensor also exhibited good anti-fouling ability, and it was capable of assaying target AFP in 10% (V/V) human serum samples, indicating highly feasible potential for clinical diagnosis.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·5030-5037
·
IF 3.864
Qiong Wu,Fengxia Zhang,Huijuan Li,Zhihua Li,Qi Kang,Dazhong Shen
Herein, we report a ratiometric photoelectrochemical (PEC) immunosensor coupled with secondary antibodies–Co3O4 nanoparticle conjugates (Ab2–Co3O4 NPs) for signal amplification. The Ab1-g-C3N4@TiO2 NTs and BSA-g-C3N4@TiO2 NTs act as the sensing and reference photoelectrodes, where Ab1, BSA and TiO2 NTs are the capture antibodies, bovine serum albumin and TiO2 nanotube arrays, respectively. In the presence of target Ag, the sensing PEC photocurrent decreases due to the steric hindrance of Ab1 molecules, which increases the inner resistance of the photoelectrode. The signal-off response is enhanced further using Ab2–Co3O4 NP conjugates due to their steric hindrance effect and the consumption of electron donors by the Co3O4 NPs. Furthermore, the performance of the ratiometric PEC platform is tested using alpha-fetoprotein (AFP) as a model antigen. Under the optimal conditions, the concentration of AFP is detected in the range of 0.4 pg mL−1 to 40 ng mL−1 with a detection limit of 0.2 pg mL−1. This ratiometric strategy is beneficial to improve the reliability and anti-interference ability of PEC immunosensors.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 20
·2977-2982
·
IF 1.9
Yuanhui Ma,Liping Zhang,Lei Wang,Lun Wang,Hongqi Chen
A novel surface plasmon resonance (SPR) enhanced upconversion (UC) luminescence system was designed for the detection of α-fetoprotein (AFP) by using gold nanoparticle (NP) coated NaYF4:Yb,Er,Gd upconversion nanoparticles (UCNPs) as probes. The gold NP coated NaYF4:Yb,Er,Gd UCNPs had a strong emission at 665 nm and were modified with poly(acrylic acid) (PAA) before being conjugated with anti-AFP (Ab1). When the AFP antigens were added to the system, the binding affinity between AFP and Ab1 resulted in a reduced distance between the gold NP coated UCNPs. The UC luminescence intensity of this system was enhanced because of the SPR properties of the gold NPs. The sensing system exhibited a high sensitivity, good specificity, and wide linear range, 0.098–15.0 ng mL−1 for the detection of AFP (R = 0.99), with a detection limit as low as 0.095 ng mL−1 under optimal conditions. The method was successfully applied to AFP sensing in human serum samples.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Xixuan Gu,Qingning Li,Xiaohui Song,Chen Chen,Xiaobing Yin,Jiayu Shen,Rui Yu,Keqi Tang
The concentration of alpha-fetoprotein (AFP) generally increases in the serum of hepatocellular carcinoma (HCC) patients. Hence, AFP is a biomarker for HCC detection, diagnosis, and treatment. However, the sensitivity is unsatisfactory. Here a fluorescent probe based on CdTe quantum dots (QDs-AFPAb) was prepared and a simple sensitive fluorescence immunoassay on nitrocellulose membranes (NFIA) was developed for alpha-fetoprotein. This method exhibited a low detection limit of 2.18 pg/mL with a linear range from 10 to 1.0 × 107 pg/mL. In addition, the sensitivity allowed the identification of HCC and non-HCC serum. This method is sensitive, easy, and low-cost for the diagnosis of HCC.
研究领域:
生物技术
·
分析化学
·
医学诊断
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Peipei Li,Zhenjuan Xu,Lili Zhao,Haoyu Chen,Xiaohua Zhu,Youyu Zhang,Meiling Liu,Shouzhuo Yao
Development of nanoluminophores with high efficiency, good stability, and excellent electronic transfer ability is one of the key challenges in electrochemiluminescence (ECL). Here, a porphyrin-based nanomaterial named Fc-TCPP was synthesized by self-assembly of 1,1′-ferrocenedicarboxylic acid (Fc(COOH)2) and tetrakis-(4-carboxyphenyl) porphyrin (TCPP) under infrared heating light. By tuning the ratio of the precursors, the morphology of Fc-TCPP can be regulated from primitive nanospheres to thorn-ball flowers decorated with branches and particles, and the possible formation mechanism was proposed. Nano-boron carbide (B4C) with carbon defect sites further boosts the ECL performance of Fc-TCPP, and thereby, an ECL biosensor for AFP was constructed innovatively. This study provides a simple and effective strategy for the preparation of ECL nanoluminophores taking the advantages of ferrocene and TCPP, opening a new horizon to design porphyrin-based nanomaterials with various structures and expanding their application in biosensing and disease diagnosis.
研究领域:
材料科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Haolin Xiao,Shanshan Wei,Miao Gu,Zhencheng Chen,Liangli Cao
Alpha-fetoprotein (AFP) plays an important role as a marker in the clinical diagnosis of cancer. However, the sensitive detection of AFP in clinical samples is still challenging. Therefore, we developed a highly sensitive electrochemical immunosensor for the effective detection of AFP. In this study, thionine (Thi) and gold nanoparticles (AuNPs) co-modified reduced graphene oxide-tetraethylene pentamine (rGO-TEPA-Thi-Au), which was used as the immunosensor platform to fix the primary antibody (Ab 1 ), leading to a large improvement in the sensitivity of the immunosensor. Furthermore, ordered mesoporous carbon (CMK-3) modified with AuPt binary nanoparticles ( [email protected] ) was used as the signal probe for further signal amplification because CMK-3 can provide a large specific surface area for in-situ reduction of AuPtNPs with good conductivity. Therefore, a dual signal amplification was observed in the amperometric i-t curve, and the detection limit of the proposed immunosensor was effectively enhanced. The constructed immunosensor showed a wide linear range of 0.005 to 100 ng mL −1 and a low detection limit of 0.0022 ng mL −1 at S/N = 3. Furthermore, the proposed immunosensor exhibited excellent stability and recovery, demonstrating its potential for clinical applications.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
电化学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Shen Liyue,Peng Tao,Dong Jiahui,Liang Zhanwei,Si Jihao,Ye Hua,Xie Jie,Yu Xiaoping,Dai Xinhua
Biomarkers screening is a benefit approach for early diagnosis of major diseases. In this study, magnetic nanoparticles (MNPs) have been utilized as labels to establish a multi-line immunochromatography (MNP-MLIC) for simultaneous detection of carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA 19–9), and alpha-fetoprotein (AFP) in a single serum sample. Under the optimal parameters, the three biomarkers can be rapidly and simultaneously qualitative screening within 15 min by naked eye. As for quantitative detection, the MNP-MLIC test strips were precisely positioned and captured by a smartphone, and signals on the test and control lines were extracted by ImageJ software. The signal ratio of test and control lines has been calculated and used to plot quantitative standard curves with the logarithmic concentration, of which the correlation coefficients are more than 0.99, and the limit of detection for CEA, CA 19–9, and AFP were 0.60 ng/mL, 1.21 U/mL, and 0.93 ng/mL, respectively. The recoveries of blank serum were 75.0 ~ 112.5% with the relative standard deviation ranging from 2.5 to 15.3%, and the specificity investigation demonstrated that the MNP-MLIC is highly specific to the three biomarkers. In conclusion, the developed MNP-MLIC offers a rapid, simple, accurate, and highly specific method for simultaneously detecting multiple biomarkers in serum samples, which provides an efficient and accurate approach for the early diagnosis of diseases. Graphical Abstract
研究领域:
医学诊断
·
纳米技术
·
生物标志物筛查
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）