FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingrui Zhang,Xiaoxiao Zhang,Qibao Jiang,Xiaoge Li,Jing Xu,Miaomiao Jiang
Methylglyoxal (MGO) is a reactive carbonyl compound that forms advanced glycation end products (AGEs), which are associated with diseases such as diabetes, cancer, and Alzheimer's disease. Turmeric, which contains bioactive diarylheptanoids, has compounds like curcumin that can trap MGO and inhibit the formation of AGEs. However, diarylheptanoids suffer from poor stability and solubility, complicating their use in standard methods. Natural deep eutectic solvents (NADES), particularly the BG12–10 % system (betaine and glycerol mixed in a 1:2 ratio with 10 % water added), can improve these properties by enhancing solubility and stability, thus enabling more accurate reaction kinetics. The NADES extract of turmeric can also directly react with MGO, simplifying the experimental process. Liquid chromatography-mass spectrometry has identified 21 diarylheptanoids in turmeric, 10 of which can trap MGO, curcumin being the only one previously reported in the literature.
研究领域:
生物化学
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药理学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 2
·e70046
·
IF 3.4
En-Zhong Chi,Li Wang,Jia-jian Pan,Qiang Zhang,Jiangxiong Zhu,Xin-huai Zhao
This study investigated the structural characteristics and hypoglycemic activity of polysaccharides isolated from Ganpu tea (GPT) in different storage periods. Three polysaccharide fractions (GPTPS1-E2, GPTPS3-E2, and GPTPS5-E2, representing 1, 3, and 5 years of storage, respectively, where GPTPS is Ganpu tea polysaccharides) were extracted and purified from GPT. Chemical composition analysis revealed that all fractions were predominantly acidic polysaccharides with total sugar content exceeding 90%. Monosaccharide composition analysis showed galacturonic acid as the primary component, along with significant amounts of galactose and arabinose. Molecular weight distribution showed that different fractions had distinct distributions, with GPTPS1-E2 and GPTPS3-E2 showing the highest and lowest molecular weights, respectively. Fourier-transform infrared and nuclear magnetic resonance spectroscopy provided insights into the structural characteristics of the polysaccharides. Scanning electron microscopy and X-ray diffraction analyses revealed distinct surface morphologies and crystalline structures. The hypoglycemic activity was evaluated using a Caco-2 cell model, demonstrating potential antidiabetic properties via the IRS1-GLUT2-SGLT1 axis. Comparative analysis revealed that storage time could influence the structural characteristics and hypoglycemic activity of GPTPSs, with a certain storage time generally associated with the transformation of structural characteristics related to hypoglycemic activity and the enhanced hypoglycemic effect. This research contributes to the understanding of GPTPS and their potential applications in functional foods and nutraceuticals.
研究领域:
食品科学
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营养保健品
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生物化学
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药理学
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DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Kong Linghong,Wang Hui,Jiang Weiwei,Chen Yun,Du Zhuoling,Zhang Qiong,Dong Jianyue,Zeng Changchun,Liu Hanping
Advanced Glycation End Products (AGEs) are the molecular mediators that contribute to the progression of diabetic complications. However, there is a paucity of research on effective AGEs inhibitors and strategies for blocking the AGEs-RAGE pathway. To solve the problem, we synthesized polymetformin (PL), which for the first time proved the ability to inhibit AGEs formation and block the AGEs-RAGE pathway to protect the vascular from being damaged. Based on gene ontology (GO) analysis and Kegg enrichment, we found that PL could antagonize AGEs. Molecular docking and dynamics analyses showed that PL formed stable structures with AGEs through electrostatic interactions and hydrogen bonding. PL treatment altered AGEs structures have been proven in FT-IR results. The study discovered that PL interacted with AGEs via both non-covalent and covalent modifications, altering AGEs’ binding sites and antagonizing the AGEs-RAGE pathway. Immunofluorescence assays indicated lower levels of RAGE, IL-1β, and TNFα, while ROS assays demonstrated lower ROS levels, highlighting PL’s inhibitory effects and biocompatibility. Our work underscores PL’s potential to treat diabetic complications by elucidating its mechanism of action against the AGEs-RAGE pathway and inflammatory factors for the first time. This research provides insights for managing chronic illnesses linked to the AGEs-RAGE pathway beyond diabetes complications.
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PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2022
·Vol. 16
·期 4
·3182-3195
·
IF 3.006
Wu Ding-Tao,Li Fen,Feng Kang-Lin,Hu Yi-Chen,Gan Ren-You,Zou Liang
This study aimed to reveal the impacts of different extraction technologies on the physicochemical properties and in vitro biological functions of dandelion leaf polysaccharides (DLP). The results revealed that the yields (8.69–11.25%), contents of uronic acids (10.29–16.64%), contents of conjugated polyphenolics (26.56–57.67 mg GAE/g), and molecular weights (fraction 1, 4.36–7.68 × 10 4 Da; fraction 2, 3.22–4.56 × 10 4 Da) of DLP were notably affected by different extraction procedures, whereas the primary structures (rhamnogalacturonan I, homogalacturonan, and arabinogalactan segments) of DLP were overall stable according to the FT-IR and NMR analysis. Additionally, the in vitro biological functions of DLP were also obviously affected by different extraction procedures. More specifically, the strongest biological functions, including in vitro antioxidant capacities (ABTS, IC 50 = 0.54 ± 0.01 mg/mL; DPPH, IC 50 = 1.03 ± 0.02 mg/mL; Nitric oxide, IC 50 = 0.21 ± 0.01 mg/mL), inhibitory effects on the formation of advanced glycation end products (IC 50 = 1.46 ± 0.04 mg/mL), and inhibitory effects against α-glucosidase (IC 50 = 14.28 ± 0.28 μg/mL), were found in DLP-P extracted by the pressurized hot water extraction among all selected technologies, which might be owing to its high contents of uronic acids and conjugated polyphenolics according to the heat map analysis. The findings can provide fundamental knowledge to select desirable extraction technologies for preparation of DLP. Indeed, the results are beneficial to well understanding the potential structure-function relationships of DLP. Graphical abstract
研究领域:
食品科学
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植物化学
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生物化学
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DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2014
·
IF 1.178
Guang-Yue Hou,Lu Wang,Shu Liu,Feng-Rui Song,Zhi-Qiang Liu
The formation of advanced glycation end-products (AGEs) and aldose reductase (AR) activity have been implicated in the development of diabetic complications. Our study sought to characterize the capacities of eleven herbal extracts against the formation of AGEs and the AR activity. An ultrahigh performance liquid chromatography and tandem mass spectrometry (UPLC–MS/MS) method was used for the detection of AR activity and the screening of AR inhibitors in this research. The amount of sorbitol from each analyte was directly detected using the multiple reaction monitoring mode and the sorbitol level could be reduced via the addition of an inhibitor. Moreover, the BSA/glucose (fructose) system was applied to investigate their inhibitory activities of AGEs formation in glycation model reactions. Compared with other screened herbs used in our study, Flos Sophorae Immaturus and Radix Scutellariae seemed to be more effective on inhibiting the formation of AGEs and AR activity. The inhibiting capacities of herbal extracts against AR activity and AGEs formation may be correlated with the bioactive components of the herbal extracts. The differences were correlated with the amount of polyphenol and flavonoid components. In the study, we have investigated the potential anti-hyperglycemic bioactivity of eleven herbal extracts in vitro , which could provide a reference for further in vivo research in the prevention and treatment of diabetic complications.
研究领域:
药理学
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生物化学
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DOI
高影响因子
Journal of Agriculture and Food Research
·
2026
·
IF 7.2
Yang Zhang,Zhengzheng Ying,Li Sun,Zheping Yu,Yuyong Wu,Haiying Ren,Xingjiang Qi,Shuwen Zhang,Senmiao Liang
Tree age influences plant secondary metabolism, but its effect on antioxidant components in Chinese bayberry leaves remains unclear. Combining chemical assays and widely targeted metabolomics, we analyzed bayberry leaves of different ages, including young (<100 years) and mature (∼300, ∼400, and ∼500 years old) groups. We found that the contents of polyphenols and flavonoids significantly increased with tree age, which in turn enhanced their antioxidant activity. The 500-year-old leaves exhibited the highest activity. Metabolomic analysis identified a total of 3152 metabolites, with terpenoids, flavonoids, and phenolic acids being the predominant classes, collectively representing 50.44% of the total identified metabolites (20.43%, 20.11%, and 9.90%, respectively). Subsequent multivariate analysis revealed that the “Subclass 1” metabolites-comprising phenolic acids, flavonoids, and terpenoids-were positively correlated with antioxidant activity. Furthermore, two compounds, picfeltarraenin X and isothankunic acid, were identified in Chinese bayberry leaves for the first time. These results demonstrate that tree age enhances antioxidant capacity by regulating the accumulation of key secondary metabolites, predominantly terpenoids, flavonoids, and phenolic acids, thereby providing a scientific basis for the high-value utilization of bayberry leaves.
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高影响因子
Frontiers in Veterinary Science
·
2024
·
IF 2.6
Xu Changzhi,Yang Xianshu,Sui Heming,Tong Xu,Zhang Dandan,Zheng Xianrui,Jiao Jun,Wang Chonglong,Cao Zubing,Zhang Yunhai
According to previous studies, the quality and fertilization rate of fresh sperm from boars of different ages were significantly different. However, the difference of freeze–thaw sperm quality and fertility in boars of different ages is unclear. In this study, boars of a Chinese native breed were assigned into two groups. Each group consisted of five boars aged aged either 2–3 years (young boars = YB) or 5–6 years (aging boars = AB) A total of 60 ejaculates for each group were collected and cryopreserved. Semen quality and in vitro fertility of post-thaw sperm was evaluated. The results showed that the concentration and motility of fresh sperm collected from AB were similar to YB, but their semen volume was higher than that in YB (p < 0.05). Frozen–thawed sperm of AB had lower viability than YB, and higher abnormal rate and reactive oxygen species (ROS) levels of YB (p < 0.05). There was no effect of the age on post-thaw sperm motility and time survival. Functional assessments indicated that increasing age markedly compromises the integrity of the sperm plasma membrane and acrosome, as well as mitochondrial functionality post-thaw, albeit without affecting DNA integrity. Furthermore, increasing age of boars reduces the ability of sperm to bind to the oocyte zona pellucida after thawing, delaying the time of the first embryo cleavage after fertilization. Finally, the early developmental efficiency of in vitro fertilized embryos progressing from 4-cell to blastocyst derived from post-thaw sperm in AB significantly decreased compared to those from YB (p < 0.05). Taken together, these results suggest that increasing age in boars impairs the quality and in vitro fertility of frozen thawed sperm.
研究领域:
动物科学
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生殖生物学
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兽医学
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DOI
PubMed
高影响因子
CELL RESEARCH
·
2025
·
IF 25.9
Hu Hui-Hui,Wang Xuefeng,Lan Bin,Cheng Haili,Wen Hong,Chen Fangfang,Wu Jianfeng,Li Mengqi,Chen Jiazhou,Zhang Jinhui,Chen Dongxu,Lin Shiyu,Lin Jieyu,Yang Mingyang,Wu Zhenhua,Zheng Zhong-Zheng,Chen Fuqing,Zhou Jianyin,Chen Gang,Chen Yu,Deng Xianming,Zhang Chen-Song,Liu Jingfeng,Lin Sheng-Cai
Dysregulated metabolism in tumor tissues and para-tumor tissues alike can lead to immunosuppression, which may underlie cancer development. However, metabolic intervention as a therapeutic strategy has been of no avail. In this study, we explored the anti-cancer therapeutic effect of aldometanib, which specifically targets lysosome-associated aldolase to mimic glucose starvation and thereby activates lysosomal AMP-activated protein kinase (AMPK), a master regulator of metabolic homeostasis. We show that aldometanib inhibits the growth of hepatocellular carcinoma (HCC) in an AMPK-dependent manner, allowing hepatoma-bearing mice to survive to mature ages, although aldometanib does not possess cytotoxicity toward HCC or normal cells. Intriguingly, aldometanib exerts anti-cancer effects only in immune-competent host mice, but not in immune-defective mice. We also found that HCC tissues in aldometanib-treated mice were massively infiltrated with CD8 + T cells, which was not seen in mice with liver-specific knockout of AMPKα . Our findings thus suggest that the metabolic regulator AMPK rebalances the tumor microenvironment to allow cytotoxic immune cells inside the body to eliminate cancer cells and effectively contain the tumor tissues. The finding that metabolic intervention can make cancer a lifelong manageable disease may usher in a new era of cancer therapy.
研究领域:
肿瘤学
·
免疫代谢
·
癌症免疫治疗
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分子生物学
·
代谢调控
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产品关联
DOI
PubMed
高影响因子
eLife
·
2024
·
IF 6.4
Junjun Yao,Shaoxing Dai,Ran Zhu,Ju Tan,Qiancheng Zhao,Yu Yin,Jiansen Sun,Xuewei Du,Longjiao Ge,Jianhua Xu,Chunli Hou,Nan Li,Jun Li,Weizhi Ji,Chuhong Zhu,Runrui Zhang,Tianqing Li
While accumulated publications support the existence of neurogenesis in the adult human hippocampus, the homeostasis and developmental potentials of neural stem cells (NSCs) under different contexts remain unclear. Based on our generated single-nucleus atlas of the human hippocampus across neonatal, adult, aging, and injury, we dissected the molecular heterogeneity and transcriptional dynamics of human hippocampal NSCs under different contexts. We further identified new specific neurogenic lineage markers that overcome the lack of specificity found in some well-known markers. Based on developmental trajectory and molecular signatures, we found that a subset of NSCs exhibit quiescent properties after birth, and most NSCs become deep quiescence during aging. Furthermore, certain deep quiescent NSCs are reactivated following stroke injury. Together, our findings provide valuable insights into the development, aging, and reactivation of the human hippocampal NSCs, and help to explain why adult hippocampal neurogenesis is infrequently observed in humans.
研究领域:
神经科学
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分子生物学
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干细胞生物学
·
发育生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 7
·4065-4075
·
IF 3.612
Jianling Wei,Zhengyang Wu,Tingting Chai,Fanyu He,Yuewen Chen,Xiuping Dong,Yugang Shi
Summary This study aimed to elucidate the information mechanism of advanced glycation end products (AGEs) in sturgeon fillets, and its inhibition by polyphenol. The results revealed that LTVH fillets (processed using low temperature vacuum heating method, LTVH) did not have lower values of AGEs than those treated using TC method (a typical thermal procedure using boiling water at 100 °C). The addition of tea polyphenol, on the other hand, drastically reduced the AGEs levels in LTVH fillets. An in vitro model including sturgeon myofibrillar protein and glucose was employed in this work to evaluate the inhibition of AGEs by tea polyphenol. The results showed that free Arginine was susceptible to interaction with glucose, which caused the development of AGEs. However, the addition of tea polyphenol competed with glucose for binding to free Arginine, leading to a substantial decrease in free Arginine concentration and an increase of free amino loss rate, resulting in the inhibition of AGEs generation. The LTVH technique, in combination with tea polyphenol, can be used to prevent the production of AGEs on fillets during the thermal treatment.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Xie Mengdi,Dai Haibo,Liu Jiaxin,Xue Zhe,Chen Yi,Liang Xuan,Mou Haiyan,Sun Hui,Ao Tianqi,Li Yunzhen,Chen Wenqing
To decipher the Cd hyperaccumulation and tolerance mechanisms of plants and increase phytoremediation efficiency, in this study, the physiological effects induced by environmentally relevant concentrations (0, 25 and 200 mg/kg) of Cd were characterized in Amaranthus hypochondriacus (K472) at three growth stages using LC/MS-based metabolomics. Metabolomic analysis identified 31, 29 and 30 significantly differential metabolites (SDMs) in K472 exposed to Cd at the early, intermediate and late stages of vegetative growth, respectively. These SDMs are involved in nine metabolic pathways responsible for antioxidation, osmotic balance regulation, energy supplementation and the promotion of metabolites that participate in phytochelatin (PC) synthesis. K472 at the intermediate stage of vegetative growth had the strongest tolerance to Cd with the combined action of Ala, Asp and Glu metabolism, purine metabolism, Gly, Ser and Thr metabolism and Pro and Arg metabolism. Among these crucial metabolic biomarkers, purine metabolism could be the primary regulatory target for increasing the Cd absorption of K472 for the restoration of Cd-contaminated soil.
研究领域:
植物学
·
环境科学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Huihui Yang,Xiaosen Bai,Baolong Feng,Qinghua Wang,Li Meng,Fengzhong Wang,Yutang Wang
Advanced glycation end products (AGEs) are associated with the occurrence of human chronic diseases, and exist commonly in thermally processed foods, such as infant formula. Existing research mainly focuses on the discrete simulation system, which is time-consuming and challenging, but accumulates of a large amount of valuable data. This study aimed to propose a specific Molecular Transformer-based model trained on the data curated from literature to predict the chemical reaction of AGEs, and apply it to infant formula to observe which new reactions could generate AGEs. The model achieved top-3 accuracy of 76.0% on the total dataset. Based on the model prediction results, five reactions were selected for experimental verification, and four of them were consistent with the model prediction results. This prospective study might potentially revolutionize the discovery of AGEs reactions and provide theoretical guidelines for designing a safer infant formula.
研究领域:
食品科学
·
生物信息学
·
医学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）