ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 7
·1955-1966
·
IF 4.157
Chen Shibing,Zheng Sining,Jiang Shengjie,Guo Hongyu,Yang Fafu
The selective detection of salicylaldehyde skeleton is of great significance in phytochemistry and biological research but rarely reported. In this research, a simple and highly selective “turn-on” fluorescence sensor ( CDB-Am ) for salicylaldehyde skeleton was developed based on switch of photoinduced electron transfer (PET) and aggregation-induced emission (AIE). CDB-Am bearing amino-cyanodistyrene structure responded to salicylaldehyde in the range of 3.1 to 40 μM with a detection limit of 0.94 μM. The sensing process of formation of Schiff-base adduct CDB-SA was confirmed by 1 H NMR, MS, and FT-IR spectra, revealing that a recovered AIE property accounted for the turn-on fluorescence response of CDB-Am and the intramolecular hydrogen bonding played a crucial role in the disruption of PET process. This sensing ability was successfully applied for both fluorescence qualitative test of salicylaldehyde skeleton on TLC analysis and quantitative detection of salicylaldehyde skeleton with good accuracy in the root bark of Periploca sepium , suggesting the extensive applications in phytochemistry and traditional Chinese herbal medicine. Furthermore, CDB-Am exhibited the first excellent fluorescence imaging ability in detecting salicylaldehyde skeleton in a living system. This work supplied a new strategy of preparing a novel “turn-on” fluorescence probe for detecting salicylaldehyde skeleton in complex environments and living bodies. Graphical abstract
研究领域:
分析化学
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生物化学
·
药学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 39
·15903-15914
·
IF 5.7
Mengyuan Niu,Yuliang Yang,Yue Sun,Yu Hu,Kaiyue Song,Xiaoxia Sun
A novel chiral fluorescent probe, tetraphenylvinyltriazole-ribosyl ribose derivative (β,D)-RIB-TPE, was prepared by Click reaction. It can self-assemble into amphiphilic micron vesicles in methanol solution. (β,D)-RIB-TPE has good AIE properties with a large Stokes shift (redshift 61.8 nm), self-assembles into J-aggregates in a mixed solution with 98% water content, and exhibits a relative fluorescence intensity αAIE = 24, emitting bright blue fluorescence. In addition to the micron vesicles, (β,D)-RIB-TPE self-assembled into J-aggregates in different mixtures, showing different morphologies, such as a cross-linked network structure and an irregular bulk stacking structure. The fluorescent probe (β,D)-RIB-TPE could achieve high efficiency and rapid multi-channel detection of clopidogrel S-(+)-sulfate by fluorescence chromatography and CD spectroscopy. Infrared, nuclear magnetic titration, scanning electron microscopy and DFT calculations confirmed that the probe (β,D)-RIB-TPE recognized S-(+)-clopidogrel sulfate by an ICT mechanism. (β,D)-RIB-TPE and S-(+)-clopidogrel sulfate were co-assembled in methanol to form single-target drug-carrying micron vesicles with a smaller particle size than the self-assembled (β,D)-RIB-TPE vesicles and good drug embedding capacity.
研究领域:
材料科学
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生物医学工程
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药学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Chao Peng,Xinyu Ma,Dan Lin,Xiaowen Feng,Huan Yu,Yinhui Li
Mitophagy , a specialized form of autophagy, holds the key to cellular metabolism and physiology. Viscosity is a significant marker for visualization of the mitophagy process in real-time. Hence, development of well-performing viscosity probe is beneficial to study mitophagy-related dynamic physiological and pathological processes. Here, a new strategy was proposed by combination of AIE property and molecular rotors to design novel viscosity probe. The probe named TPA-Py was obtained by Knoevenagel condensation reaction of AIE unit and pyridine salt, which giving the probe excellent near-infrared emission, good water-solubility and mitochondrial targeting ability. Most importantly, TPA-Py owns two rotatable parts of triphenylamine and double bond , enabling the probe to equip with AIE property and sensitive recognition units for viscosity. With the environmental viscosity increasing, the rotation of the molecular rotor and the AIE unit is restricted effectively, the probe displayed strong fluorescence. Then, TPA-Py was successfully employed for monitoring the mitophagy process in A549 cells by imaging viscosity alterations. As mitophagy constitutes an important consideration in the pathogenesis of drug-induced liver injury, TPA-Py was also applied to explore the variation of viscosity in production and remediation pathways of APAP-induced liver injury. These results demonstrated that TPA-Py was a highly sensitive viscosity probe which holds great potential of biological applications.
研究领域:
生物化学
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医学影像
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细胞生物学
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DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 1
·e4675
·
IF 3.2
Gerile Oudeng,Junguo Ni,Hao Wu,Honglian Wu,Mo Yang,Chunyi Wen,Yuanwei Wang,Hui Tan
The coronavirus disease-2019 pandemic reflects the underdevelopment of point-of-care diagnostic technology. Nuclei acid (NA) detection is the “gold standard” method for the early diagnosis of the B.1.1.529 (Omicron) variant of severe acute respiratory syndrome-coronavirus disease-2. Polymerase chain reaction is the main method for NA detection but requires considerable manpower and sample processing taking ≥ 3 h. To simplify the operation processes and reduce the detection time, exonuclease III (Exo III)-aided MoS 2 /AIE nanoprobes were developed for rapid and sensitive detection of the oligonucleotides of Omicron. Molybdenum disulfide (MoS 2 ) nanosheets with excellent optical absorbance and distinguishable affinity to single-strand and duplex DNAs were applied as quenchers, and aggregation-induced emission (AIE) molecules with high luminous efficiency were designed as donor in fluorescence resonance energy transfer-based nanoprobes. Exo III with catalytic capability was used for signal amplification to increase the sensitivity of detection. The composite nanoprobes detected the mutated nucleocapsid (N)-gene and spike (S)-gene oligonucleotides of Omicron within 40 min with a limit of detection of 4.7 pM, and showed great potential for application in community medicine.
研究领域:
生物医学工程
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分子生物学
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病毒学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Siqi Chen,Ruyan Xie,Miaomiao Tian,Rui Yan,Zizhun Wang,Ruifeng Xie,Fang Chai
Heavy metal pollution is life-threatening, the detection of heavy metals is crucial to human health and environment. In this work, AIE featured LA-stabilized gold nanoclusters (NL-AuNCs) were designed and fabricated by modulating precursors in various reaction conditions, and utilized as an efficient fluorescent sensor for portable detecting Ag + with high sensitivity and selectivity. The NL-AuNCs exhibited intense red fluorescence at 625 nm via the secondary reducing agent N-acetyl-L-cysteine (NAC), achieving a quantum yield (QY) of 15.7 %. Notably, the introduction of Ag + enhanced the red fluorescence intensity of NL-AuNCs on account of the aggregation-induced emission (AIE) process, rendering NL-AuNCs uniquely capable of detecting Ag + with the ultralow detection limit of 1.3 nM within a wide concentration range of 0.002–200 μM. Furthermore, the handheld intelligent sensing strategy was constructed by integrating the smartphone App, which enabled swift portable monitoring and convenient readout by visual assessment of color evolution. The acceptable recoveries of 94.6 % to 114.9 % were attained from testing real water, indicating the excellent environmental tolerance of NL-AuNCs, which allowed the possibility for practical in-situ detection.
研究领域:
环境科学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiuying Liu,Yu Wang,Zexin Zhang,Lijie Zhu,Xue Gao,Keli Zhong,Xiaofei Sun,Xuepeng Li,Jianrong Li
Freshness is an important parameter of fish quality. This study aims to develop a ratiometric fluorescent sensing label that is responsive to volatile amines, affording real-time and visual detection of fish freshness. For developing of the sensing label, an aggregation-induced emissive (AIE) polymer was prepared from the stimuli-responsive polymer polymethacrylic acid and the AIE molecule tetraphenylethylene and coated on to filter paper with rhodamine B as an internal reference. By exploiting the ratiometric response, the freshness of fish could be identified clearly and easily according to the color of on-package label, which changes from pink (fresh) to dark blue. The difference was linearly correlated with the total volatile basic nitrogen (TVB-N, R 2 = 0.995 and 0.994 at 25 °C and 4 °C, respectively) in the range of 15–25 mg/100 g for the salmon samples, which indicated that the sensing label feasibly and non-destructively quantified TVB-N.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2024
·
IF 10.7
Jiahui Lin,Xing Feng,Jintao Huang,Yiwei Liu,Yongshuang Xiao,Yanfeng Li,Yonggang Min,Ben Zhong Tang
Alcohol sensing plays a critical role in medical detection and personal health management. AIE materials with high sensitivity, selectivity and fast response have been widely used in biosensing, but their application in the field of alcohol sensing still needs further research and development. Furthermore, developing flexible phase change materials (PCMs) is significant for the research of human-body thermal management. In this study, a kind of flexible polyacrylonitrile (PAN)/polyvinylpyrrolidone (PVP)/polyethylene glycol (PEG)/Py-CH (pyrene-based AIE molecule)/SiO 2 @h-BN composite fiber textile (PAB) with alcohol sensing performance, writable fluorescence property, and human body thermal management function has been prepared via electrospinning technique. The PAN/PVP fiber matrix successfully integrated AIE fluorescent sensing material and PCM into a multi-functional composite with great shape stability. Owing to the introduction of novel pyrene-based Py-CH with AIE characteristic, this innovative textile exhibited wonderful fluorescent properties, including sensitive alcohol fluorescence sensing, writable fluorescence performance and variable temperature fluorescence. Furthermore, proposed PAB textile delivered a high energy storage density of 87∼90 J/g, excellent thermal reliability, great comprehensive mechanical flexibility and enhanced thermal conductivity for flexible human body thermal management. Hence, this flexible multifunctional AIE/PCM composite sensing textiles can be widely used in alcohol sensing, fluorescence anti-counterfeiting and flexible body thermal management.
研究领域:
材料科学
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生物传感
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热管理
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纺织工程
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 35
·5571-5578
·
IF 5.687
Zhihai Cao,Xiaoqin Liang,Hangnan Chen,Meng Gao,Zujin Zhao,Xiaolong Chen,Chang Xu,Gan Qu,Dongming Qi,Ben Zhong Tang
Aggregation-induced emission (AIE) polymeric nanoparticles (NPs) with a size of several tens of nanometers were successfully prepared by miniemulsion copolymerization of methyl methacrylate (MMA) with an AIE monomer, 1-allyl-1-methyl-2,3,4,5-tetraphenylsilole (AMTPS). The prepared AIE poly(MMA-co-AMTPS) NPs exhibited strong photoluminescence with high quantum yields. Promisingly, the brightness of AIE poly(MMA-co-AMTPS) NPs, which increased linearly with an increase in AMTPS content, could be accurately modulated via the AMTPS content in the range of 0–20 wt%. In vitro cell experiments indicated that the AIE poly(MMA-co-AMTPS) NPs were promising cell staining reagents with good biocompatibility and high photostability.
研究领域:
材料科学
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纳米技术
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生物医学工程
·
细胞生物学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2017
·
IF 3.473
Yafei Wang,Wanhui Liu,Zuoming Qu,Hua Tan,Yu Liu,Guohua Xie,Weiguo Zhu
To explore the structure-property of donor-acceptor (D-A) type blue emitter, in this contribution, four star-shaped compounds of p -TPA, m -TPA, p -TPA-TPE and m -TPA-TPE using spirotriphenylamine as the donor unit and triazine as the acceptor core were designed and prepared. The tetraphenylethene (TPE) unit was introduced into the D-A molecules to enhance the emission efficiency in solid state. The photophysical properties of all compounds were comparatively studied through experimental and theoretical methods. All these D-A compounds displayed blue emission (443–487 nm) both in solution and neat film. Compared to p -TPA and m -TPA, p -TPA-TPE and m -TPA-TPE possessed clear aggregated-induced emission (AIE) property and higher emission efficiency in the solid state. Both the doped and non-doped organic light-emitting diodes (OLEDs) based on m -TPA and m -TPA-TPE were fabricated by solution-processable approach. The m -TPA-TPE based non-doped device showed a satisfying performance with a high current efficiency of 4.2 cd/A and an external quantum efficiency of 1.8% in the bluish-green region. This research demonstrates that integrating AIE unit into D-A molecule is an effective strategy for design high efficient blue emitter in non-doped OLEDs.
研究领域:
材料科学
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光学
·
化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Nuanfei Zhu,Kuijing Yuan,Dinghui Xiong,Fengxiang Ai,Kun Zeng,Biying Zhao,Zhen Zhang,Hongjun Zhao
Dietary intake is an important exposure pathway of environmental bisphenols including bisphenol S (BPS, a typical emerging organic pollutant). However, available data on the occurrence and risk assessment of BPS in food samples are limited due to the lack of high-throughput analytical methods. Herein, based on H 2 O 2 inducing fluorescence turn-off signals, a sensitive and high-throughput fluorescence enzyme-linked immunosorbent assay (FELISA) was developed for BPS investigation in beverages. In this system, the fluorescence of copper nanoclusters in ZIF-8 (CuNCs-CS@ZIF-8) could be quenched with the presence of H 2 O 2 owing to the strong oxidation, being served as signal amplification in FELISA. Meanwhile, the aggregation-induced emission (AIE) fluorescence enhancement of CuNCs-CS through the conformational locking of ZIF-8 provided an effective strategy to overcome the intrinsic limitations of FELISA including high background, serious fluorescence quenching in the detection of real samples. Thus, the integration of AIE building blocks into porous ZIF-8 showed obvious advantages in enhancing the analytical performance of FELISA. Under optimized conditions, satisfactory results with good accuracy were achieved in this proposed FELISA, which was applied for the systematic investigation of BPS in foods and further health risk assessments. The results after 100 beverages analysis displayed an average detection frequency of 55%, and the detected concentration of BPS ranged from below the limit of quantification (LOQ) to 12.08 ng/mL with a mean value of 1.717 ng/mL. Besides, the estimated daily intake (EDI) of BPS was calculated to be 0.05449–4.483 ng/kg-bw/day, in which milk–tea drinks contributed to the majority of BPS daily intake (mean EDI = 4.483 ng/kg-bw/day). And the hazard quotient (HQ) according to EDI values indicated that BPS exposure in beverages might not make a considerable impact on human health.
研究领域:
环境科学
·
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2018
·Vol. 10
·期 7
·722
·
IF 2.935
Meiran Feng,Laiping Fang,Fujun Guan,Siying Huang,Yinwei Cheng,Yancui Liang,Hefeng Zhang
A facile and efficient approach for design and synthesis of organic fluorescent nanogels has been developed by using a pre-synthesized polymeric precursor. This strategy is achieved by two key steps: (i) precise synthesis of core–shell star-shaped block copolymers with crosslinkable AIEgen-precursor (AIEgen: aggregation induced emission luminogen) as pending groups on the inner blocks; (ii) gelation of the inner blocks by coupling the AIEgen-precursor moieties to generate AIE-active spacers, and thus, fluorescent nanogel. By using this strategy, a series of star-shaped block copolymers with benzophenone groups pending on the inner blocks were synthesized by grafting from a hexafunctional initiator through atom transfer radical copolymerization (ATRP) of 4-benzoylphenyl methacrylate (BPMA) or 2-(4-benzoylphenoxy)ethyl methacrylate (BPOEMA) with methyl methacrylate (MMA) andtert-butyldimethylsilyl-protected 2-hydroxyethyl methacrylate (ProHEMA) followed by a sequential ATRP to grow PMMA or PProHEMA. The pendent benzophenone groups were coupled by McMurry reaction to generate tetraphenylethylene (TPE) groups which served as AIE-active spacers, affording a fluorescent nanogel. The nanogel showed strong emission not only at aggregated state but also in dilute solution due to the strongly restricted inter- and intramolecular movement of TPE moiety in the crosslinked polymeric network. The nanogel has been used as a fluorescent macromolecular additive to fabricate fluorescent film.Keywords:star-shaped polymeric precursor;fluorescent nanogel;AIE-active spacer
研究领域:
材料科学
·
聚合物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Cheng Li,Yu Chen,Lijun Yang,Congrou Zhang,Bin Wang,Yong Wang
“Turn-on” fluorescent materials in the absence of metal and organic solvent are highly desirable as bioprobes to selectively and sensitively detect pyrophosphate (PPi) anion. In this work, we embedded tetraphenylethene (TPE) inside the relatively rigid hyperbranched poly(amido amine) (HPA) to afford highly water soluble TPE-HPA materials, where the TPE fluorescence was pre-quenched in water by the hyperbranched structure of HPA without participation of any metal or organic solvents. The obtained TPE-HPA could be directly used as fluorescence “turn-on” bioprobe under physiological conditions to selectively detect PPi. There was a good linearity between the photoluminescence enhancement and the PPi concentration in the range of 0–1.0 μM with a limit of detection (LOD) of 6.5 nM. Compared with the other reported AIE probes for PPi detection, TPE-HPA with the optimal TPE content showed the best comprehensive performance, that is metal-free, organic-solvent free and nanomolar level LOD. The extreme selectivity and sensitivity towards PPi allowed TPE-HPA to accurately detect the PPi concentration in human urine.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）