ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Sayed Mir Sayed,Ke-Fei Xu,Hao-Ran Jia,Fei-Fei Yin,Liang Ma,Xiaodong Zhang,Arshad Khan,Qian Ma,Fu-Gen Wu,Xiaolin Lu
Pathogenic infections, particularly caused by Gram-positive bacteria (G + ), pose a serious threat to human health, and therefore the fast and accurate discrimination of G + bacteria from Gram-negative bacteria (G – ) and fungi is highly desirable. Organic molecules with facile synthesis, robust photostability, good biocompatibility, and high selectivity toward pathogens are urgently needed in the clinical diagnosis and therapy. To this end, herein we report the synthesis of two naphthalimide-based bioprobes named tetraphenylethylene-naphthalimide (TPE-NIM) and triphenylamine-naphthalimide (TPA-NIM) with aggregation-induced emission (AIE) characteristic. First, the staining capacity of the designed AIEgens toward six kinds of bacteria and two kinds of fungi was evaluated. Both TPE-NIM and TPA-NIM showed a high degree of binding/imaging selectivity for G + bacteria over G − bacteria and fungi via a wash-free protocol. Second, the two AIEgens had the ability to visualize the biofilms formed by G + bacteria ( Staphylococcus aureus ) and can quickly track the G + bacteria ( Staphylococcus aureus ) in red blood cell suspensions. Third, we have revealed that electrostatic attraction and hydrophobic interaction both contribute to the selective binding of the AIEgens toward G + bacteria. In view of the high binding/imaging specificity toward G + bacteria, low hemolysis rates, and low toxicity toward the bacterial cells, these AIEgens can be applied for the clinical detection of pathogenic infections caused by G + bacteria and broaden the theranostic applications of AIE materials.
研究领域:
生物医学工程
·
微生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ke Xue,Yongfei Zhao,Saidong Sun,Yuanhang Li,Jiankang Liang,Zhengjian Qi
Severe ferroptosis is a non-apoptotic iron-dependent cell death pathway, and extreme hypoxia and anomalous iron metabolism in tumors critically suppress the efficacy of ferroptosis therapy. Overcoming tumor hypoxia while facilitating ferroptosis is an extremely challenging task. Herein, the photosensitizer named BOTTQ with specific targeting of lipid droplet (LD) was synthesized by increasing the tail chain length of triphenylamine. The addition of alkoxy chains optimized the molecular structure and fostered inter-system crossing and type I reactive oxygen species (ROS) generation. Besides, BOTTQ yielded large amounts of type I ROS under light excitation oxidize efficiently polyunsaturated fatty acids in lipid monolayer of LD and contribute to the formation of ferroptosis substrate. Meanwhile, the generation of type I ROS accelerated the Fenton reaction, thus effectively boosting the occurrences of ferroptosis. Furthermore, the successful encapsulation of BOTTQ into DSPE-PEG-MAL material not only elevates the stable type of nanoparticle colloids, but the MAL moiety can deplete the functional thiols of glutathione in a mild environment, which leads to glutathione failure and facilitates ferroptosis. Thus tumor-specific ferroptosis-inducing strategy offers a universally applicable therapy with the capacity to enhance type I ROS production and effectively contain tumorigenesis.
研究领域:
生物医学工程
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肿瘤学
·
药理学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Binbin Wu,Qin Zhang,Min Chen,Hongchen Zhang,Lulu Zheng,Xingyu Peng,Shihua Wang,Yun-Yun Quan,Lingtian Wang,Zu-Sheng Huang,Xiaoxia Ye
Photothermal therapy (PTT) has gained significant attention as a promising cancer treatment due to its low systemic toxicity, minimal invasiveness, and other advantages. Aggregation-induced emission luminogens (AIEgens) with second near-infrared (NIR-II) fluorescence have emerged as ideal photothermal agents (PTAs), offering key benefits such as high biosafety, excellent biocompatibility, and precise tumor theranostics. Nevertheless, achieving an optimal balance between radiation-mediated NIR-II fluorescence imaging and non-radiation NIR-PTT poses a considerable challenge in designing efficient NIR-II AIEgens. In this work, we employed side chain engineering by strategically grafting n -hexyl onto the π-bridge of a conjugated D-π-A-π-D structure, leading to the development of four new AIEgens ( BQ1 ∼ BQ4 ). These compounds feature 6,7-diphenyl- [1,2,5] thiadiazolo [3,4- g ] quinoxaline as the acceptor, thiophene as the π-bridge, and phenothiazine as the donor. This molecular design enables precise regulation of backbone distortion, effectively balancing fluorescence emission and photothermal conversion. Among them, BQ1 , BQ3 , and BQ4 exhibited strong fluorescence, longer emission wavelengths, and superior photothermal conversion efficiencies (PCEs) (59.2%, 57.8%, 52% respectively), whereas BQ2 , with greater molecular distortion, showed a lower PCE of 33.4%. Notably, BQ1 nanoparticles demonstrated outstanding photothermal conversion, prolonged tumor retention, strong resistance to photobleaching in vitro and in vivo , and NIR-II imaging capability at 1055 nm. This study introduces a novel strategy for optimizing NIR-II/AIE PTAs through precise conformational control via strategic n -hexyl positioning, which remains unreported to date.
研究领域:
生物医学工程
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材料科学
·
肿瘤学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yaxin Hu,Li Xie,Qiaozhen Que,Lidan Chen,Zhaohui Sun,Yujie Tu,Rong Hu,Yong Liu,Zhiming Wang,Ben Zhong Tang
Magnetic/fluorescent barcodes combine fluorescent dyes and magnetic materials in micrometer-sized host microspheres, offering high potential for high-throughput multiplexed biodetection automatically. However, the significant fluorescence quenching effect of magnetic nanoparticles (MNPs) towards organic luminogens limits the coding capacity in practical applications. In addition, aggregation-caused quenching (ACQ) and photon reabsorption effects of organic luminogens result in unpredictable fluorescence encoding numbers, and several experimental iterations are required. Herein, a reliable and simple strategy based on large stokes shift aggregation-induced emission (AIE) luminogens/magnetic barcodes is established for multiplex detection by eliminating the negative effects aboved of multicolor organic dyes. The in-situ generation and layer-by-layer modification strategies are utilized to prepare the related microspheres, which showed lower magnetic quenching effect and energy transfer process as expected. Thus, 36 encoded peaks are feasible based on the theoretical 6 × 6 barcode matrix with two colors and six intensity levels, providing great potential for large-scale multiplex detection. Based on the developed magnetic/fluorescent barcodes the excellent detection performance (detection limits: 5.5–20.6 pg·mL −1 ) are achieved for the detection of seven common cytokines from patient serum samples. These results indicate that the characteristic of AIEgens enjoying large stokes shift could give a barcode platform greater potential in the field of precision multiplex detection in clinic.
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产品关联
DOI
高影响因子
Materials Chemistry Frontiers
·
2020
·Vol. 4
·期 9
·2635-2645
·
IF 6.788
Jin Wang,Meng Gao,Zhong-Kai Cui,Yong-Guang Jia,Sa Liu,Kai-Feng Chen,Xiaohui Chen,Yiqing Zhang,Zhou Fang,Yunhua Chen,Kaojin Wang,Huatang Zhang,Lin Wang,Li Ren
Facing the challenges posed by bacterial infection, the real-time detection of bacterial infection combined with antibacterial functions is urgently needed. Herein, we prepare thermo- and pH-responsive poly(vinyl alcohol) derivatives (PVA–TPE) bearing aggregation-induced emission luminogens of tetraphenylethene (TPE) and quaternary ammonium moieties through a facile one-pot quaternization with a tertiary amine method. Intrinsic fluorescence from TPE moieties and antibacterial functions from quaternary ammonium units can efficiently integrate the functions of “monitoring and killing of bacteria”. It is found that PVA–TPE can selectively image and kill Gram-positive bacteria at pH 7.4, while it switched to display enhanced broad-spectrum antimicrobial activity against both Gram-negative and -positive bacteria in an acidic environment (pH 5.5). Moreover, PVA–TPE exhibits low cytotoxicity on mammalian cells of L929, HepG2 and red blood cells. Furthermore, in vivo evaluations further confirmed that PVA–TPE has excellent antibacterial efficacy with no tissue damage. This study represents the first example of a pH-switchable integrated system for “monitoring and killing of bacteria” constructed through a facile one-pot quaternization method.
研究领域:
材料科学
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生物材料
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生物医学工程
·
微生物学
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DOI
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Jie Wen,Jin Tong,Ya-Lan Kou,Ji Wang,Shu-Yan Yu
Five tetraphenylethene (TPE) derivatives 1a , 1b , 2 , 3 , and 4 with adjustable stimuli-responsive characteristics were designed and synthesized in this study. Using single-crystal X-ray diffraction, the effects of substitution positions and pyrazolyl numbers on molecular packing were examined in the solid state. The findings indicate that as the pyrazolyl numbers increased, the molecular packing of the compound progressively changed from the C−H···π stacked compact mode in 1 − 3 to the X-crossing stacked loose mode in 4 , which results in the origin of various stimuli-responsive phenomena in compound 4 . This approach facilitates the preparation of multi-stimuli-responsive (thermochromism, mechanochromism, and metal ion fluorescence switch) smart fluorophores with tunable emission color. Moreover, compound 4, with an excellent fluorescence performance, exhibited three different colors when reversible mechanochromism was selected as the candidate for an optical recording material and security ink. This study proposes an effective method to guide the design of stimuli-responsive materials, which, in turn, would facilitate the development of high-contrast MC molecules, and the optical information anticounterfeiting based on grinding was proven by utilizing the grind-responsive luminescence capabilities.
研究领域:
材料科学
·
化学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Weiwei Zhang,Yongbo Wei,Fen Lin,Yinyin Yao,Xia Wang,Yongxiao Sun,Jingming Zhou,Tong Wu,Nannan Chen,Yusheng Lu,Xiaoping Wu,Lijun Xie
In this work, a new series of rofecoxib analogues were designed and synthesized by employing the migration of the donor (D) group and donor (D)–acceptor (A) switching principle to achieve ACQ–AIE conversion. Among them, when the D unit dimethylamino was anchored on the para-position of the benzene ring D, the corresponding compound L1, namely (Z)-5-(4-(dimethylamino)benzylidene)-4-(4-(methylsulfonyl)phenyl)-3-phenylfuran-2(5H)-one, was obtained exhibiting obvious aggregation-caused quenching (ACQ) behavior. Subsequently, after migrating the D group from ring D to ring B, the resulting L2 exhibits an aggregation-induced emission (AIE) effect. Meanwhile, by switching the position of the D and A units based on the structure of L1, the corresponding compound L3 also shows AIE behavior. These results demonstrate that migrating the position of the donor group and switching the positions of the donor and acceptor have significant effects on the fluorescent properties of the compounds. Moreover, single crystal X-ray analysis of L2 and L3 revealed that the stronger molecular C–H⋯π interactions contribute to greater structural rigidification, which in turn enhances their bright emission in the solid state. In addition, L2 and L3 exhibited solvatochromic, acidichromic, and trace water properties with reversible multi-stimulus behaviors. More importantly, L2 and L3 demonstrated highly specific fluorescent imaging ability (RL2 = 0.97, RL3 = 0.98) toward lipid droplets in living MCF-7 cells, with low cytotoxicity. In summary, the creative design concepts of adjusting the position of D and switching the positions of D and A units, as well as the ACQ to AIE conversion provide alternative ways for developing new multifunctional AIEgens, L2 and L3.
研究领域:
有机化学
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生物医学
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材料科学
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 33
·期 3
·955-963
·
IF 2.525
Zeng Xiaohui,Wang Hailong,Zeng Yanbo,Yang Yiwen,Zhang Zulei,Li Lei
Insulin, the only hormone regulating blood glucose level, is strongly associated with diabetes and its complications. Specific recognition and ultrasensitive detection of insulin are of clinical significance for the early diagnosis and treatment of diabetes. Inspired by aggregation-induced emission, we presented a turn-on label-free fluorescence aptasensor for insulin detection. Quaternized tetraphenylethene salt was synthesized as the fluorescence probe. Guanine-rich aptamer IGA3 was selected as recognition element. Graphene oxide was chosen as the quencher. Under optimized conditions, the fluorescence aptasensor displayed a wide linear range (1.0 pM–1.0 μM) with a low limit of detection (0.42 pM). Furthermore, the aptasensor was successfully applied to detect insulin in human serum. Spiked recoveries were obtained in the range of 96.06%–104.26%. All these results demonstrated that the proposed approach has potential application in the clinical diagnostics of diabetes.
研究领域:
生物化学
·
医学诊断
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2018
·Vol. 10
·期 7
·786
·
IF 2.935
Rui Jiang,Na Liu,Fan Li,Wensheng Fu,Yun Zhou,Yan Zhang
Herein, a novel on-off-on fluorescent chemosensor for copper (II) ion (Cu2+), iron (III) ion (Fe3+) and cysteine is developed simply by the nano-precipitation method. The prepared organic dots with AIEgens (AIE dots) are advantageous over other metal ions in detecting Cu2+, Fe3+with high selectivity and sensitivity by forming agglomerations (on-off). The agglomerations formed by AIE dots and Cu2+redistributed and the fluorescence was obviously recovered in the presence of cysteine (off-on). This sensor has a wide linear range for Cu2+, Fe3+and cysteine. The fluorescent detection limits of AIE dots are calculated to be 107 nM for Cu2+, 120 nM for Fe3+and 78 nM for cysteine, respectively. These results indicate that the AIE dots can be used as a potential probe for Cu2+, Fe3+and cysteine detection.Keywords:fluorescent chemosensor;nanoparticle;aggregation-induced emission;ion detection;cysteine detection
研究领域:
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Xuehong Min,Yuan Zhuang,Zhenyu Zhang,Yongmei Jia,Abdul Hakeem,Fuxin Zheng,Yong Cheng,Ben Zhong Tang,Xiaoding Lou,Fan Xia
We demonstrate an ultrasensitive microRNA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. Furthermore, the assay could accomplish various detection limits by tuning the reaction temperature due to the different activity of exonuclease III corresponding to the diverse temperature. Specifically, 1 pM miR-21 can be detected in 40 min at 37 °C, and 10 aM (about 300 molecules in 50 μL) miR-21 could be discriminated in 7 days at 4 °C. The great specificity of the assay guarantees that the real 21 urine samples from the bladder cancer patients are successfully detected by our method.
研究领域:
癌症研究
·
分子生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yan Zhang,Xiaolong Xie,Song Zhao,Rui Jiang,Wenjing Qi,Lianzhe Hu
Dissolved ammonia and trinitrophenol (TNP) in water represent a critical environmental and safety challenge. Under slightly acidic conditions, these two compounds spontaneously react to form crystalline ammonium picrate—a highly shock-sensitive explosive compound. This poses explosion risks during wastewater transport, treatment, and sludge handling processes. Existing detection methods rely on complex laboratory instrumentation and cannot provide simultaneous, real-time on-site monitoring. Therefore, there is an urgent need for on-site and simultaneous monitoring of these two hazardous substances. Here, we developed an aggregation-induced emission (AIE) active molecule, DVA-4P, functionalized with four pyridine groups. Its highly twisted molecular conformation effectively suppresses π-π stacking and restricts intramolecular motions, resulting in intense solid-state fluorescence with a quantum yield of 41.87 %. This molecular design can fully leverage the dual functionality of pyridine: the protonated form (DVA-4P-H + ) enables “turn-on” fluorescence detection of ammonia, while the neutral form (DVA-4P) facilitates “turn-off” detection of TNP through electron donor-acceptor interactions. Two separate hydrogel tags are therefore prepared: one encapsulated in a PTFE membrane for selective dissolved ammonia detection, and one left unshielded for selective TNP detection. The tags can achieve high sensitivity and selectivity, with detection limits of 0.70 μg/L for ammonia and 66.2 nM for TNP. The recovery rates of both hydrogel tags in lake water exhibited excellent performance with deviations from the standard within 5 %, providing a practical, dual-mode analytical platform for the environmental monitoring and wastewater treatment.
研究领域:
环境监测
·
分析化学
·
材料科学
·
爆炸物安全
·
废水处理
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Shanting Liu,Xi Kang,Bumeng Guo,Zewei Song,Shuang Li,Yinghan Li,Sheng Hua Liu,Zhao Chen
Due to their distinctive stimulus-responsive properties, materials exhibiting hypsochromic mechanofluorochromism (MFC) or force-induced substantial weakening of fluorescence intensity have demonstrated remarkable application potential in the field of information security. This study designed and synthesized two novel fluorene-based dinuclear gold(I) complexes, 1 and 2 . Both molecules exhibited aggregation-induced emission (AIE) characteristics. Specifically, complex 1 showed reversible hypsochromic MFC. In contrast, complex 2 exhibited force-induced substantial weakening of fluorescence intensity. It is worth noting that 2 is the first example of gold(I)-based luminogen capable of simultaneously exhibiting AIE and force-induced substantial weakening of fluorescence intensity. Based on the force-induced significant fluorescence attenuation feature of luminogenic complex 2 , an interesting information anticounterfeiting system involving a pizza model was successfully established. This work not only reports the first example of AIE-active gold(I) complex featuring anisotropic mechanical force-induced pronounced fluorescence reduction but also provides a valuable reference for anticounterfeit applications of mechanically responsive luminogens.
研究领域:
材料化学
·
超分子化学
·
发光材料
·
智能响应材料
·
防伪技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）