Food Quality and Safety
·
2026
·
IF 4.9
Ba Liangjie,Luo Chenglin,Wang Xiaogang,Ye Shengjie,Luo Donglan,Cao Sen
Blueberries are prone to softening and rotting shortly after harvest, which not only complicates post-harvest preservation but also significantly impairs their quality. In this study, the regulatory mechanism of thymol pre-harvest treatment on the quality and ascorbic acid-glutathione (AsA-GSH) cycle of blueberries during storage was investigated. The results demonstrated that thymol treatment impeded the decline of total flavonoids, total phenols, reducing sugars, and starch content in blueberry fruit. In addition, thymol treatment suppressed the activities of α-amylase, β-amylase, and total amylase. In addition, thymol increased the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) and maintained high AsA and GSH contents and AsA/(AsA + DHA) and GSH/(GSH + oxidized glutathione (GSSG)) ratios in blueberries. In conclusion, thymol improved the overall postharvest antioxidant capacity of blueberries and maintained their quality by delaying the reduction of antioxidants, inducing antioxidant enzyme activities, and enhancing the metabolic activities of the AsA-GSH cycle. Therefore, thymol pre-harvest treatment can serve as an efficient and natural pre-harvest treatment agent for preserving postharvest blueberry quality, effectively maintaining the post-harvest quality of blueberries and extending their storage life.
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高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Tan Zhanming,Wu Cuiyun,Xuan Zhengying,Cheng Yunxia,Xiong Renci,Su Zhihang,Wang Desheng
Lead (Pb) is a heavy metal pollutant and negatively affects agriculture and ecosystems. Pb can cause oxidative stress and abnormal plant growth. The ascorbic acid-glutathione (AsA-GSH) cycle mainly exists in chloroplasts and resists oxidative stress, scavenges reactive oxygen radicals, and maintains normal photosynthesis. However, the dosage related effects of Pb on pakchoi photosynthesis, via oxidative stress and the AsA-GSH system, remains unclear. In this study, various Pb dosage stress models were tested (low: 300 mg/kg; medium: 600 mg/kg; high: 900 mg/kg). Pb stress induced a dose-dependent increase in Pb content in pakchoi leaves (P < 0.05). Principal component analysis showed that Se, B, and Pb were significantly and negatively correlated. Pb stress also increased MDA content and decreased antioxidant enzymes SOD, GSH-Px, and T-AOC activities (P < 0.05). We also found that Vc content, as well as the GSH/GSSG ratio, decreased. Additionally, Pb stress destroyed chloroplast structure, decreased photosynthesis indicators Pn, Tr, Gs, Ci and VPD, and attenuated Fv/Fm and Fv/Fo (P < 0.05). In the high-dose group, the contents of chlorophyll a, chlorophyll b, and carotenoids decreased significantly, while the expression of chloroplast development genes (GLK, GLN2) decreased (P < 0.05). Our data suggest that Pb stress leads to dosage-dependent, aberrant photosynthesis by inhibiting the AsA-GSH system in pakchoi. This study expands the Pb toxicology research field and provides indications for screening antagonists.
研究领域:
环境科学
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植物生理学
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毒理学
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分子生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Rongjiao Sun,Limei Zhu,Xianzhu You,Rong Chen,Jiante Li,Rongrong Pan,Yueqi Jin,Hua Zhou,Ying Tan,Jiajia Dong,Wanle Hu
5-Aminosalicylic acid (5-ASA) is widely used in the clinical treatment of ulcerative colitis (UC), but its effectiveness is limited by poor patient compliance and inadequate drug retention. Herein, a novel thermoresponsive nanogel (P(NVCL-co-GelMA)) was synthesized through the copolymerization of N-vinylcaprolactam (NVCL) and methacrylated gelatin (GelMA). This nanogel serves as an effective carrier for 5-ASA, transitioning from a sol to a hydrogel at physiological temperatures, thereby enhancing drug retention and reducing leakage. In vitro studies revealed that the nanogel has a lower critical solution temperature of approximately 34 °C and can form a gel within 60 s. It demonstrates superior bioadhesion, biocompatibility, and controlled release of 5-ASA. In a rat model of UC, the 5-ASA-loaded nanogel significantly improved weight loss and disease activity index scores compared to untreated rats. The hydrogel coating acts as a biophysical barrier on the intestinal mucosa, effectively blocking the irritation caused by metabolites and maximizing the therapeutic effect, comparable to that of commercial mesalazine enema (Salofalk). The P(NVCL-co-GelMA) nanogel represents a promising drug delivery system for the treatment of UC, offering extended drug retention, reduced leakage, and enhanced therapeutic efficacy.
研究领域:
药学
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药物递送系统
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结直肠疾病
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DOI
高影响因子
Nature Plants
·
2025
·
IF 13.6
Chen Xiuxiu,Jin Songsong,Du Hong,Liu Zebin,Hou Congcong,Tang Ling,Wang Yuanyuan,Wang Yuxi,Yang Zhaolin,Zhao Tong,Ma Jinying,Zhang Lanjun,Li Meijing,Qi Dongdong,Li Wenlong,Wang Xiaohua,Sun Runze,Moore John P.,Liu Yongxiu,Lin Jinxing,Deng Xin
Autophagy and apoplastic ascorbic acid (AsA) play important roles in plant drought tolerance. However, the trans-plasma membrane transport of AsA and its association with autophagy during drought stress are unclear. Here we report an AsA-induced autophagy pathway in plants, wherein plasma-membrane-located cytochrome b561 and DOMON domain (CYBDOM) proteins play positive roles. CYBDOM proteins from the resurrection plant Boea hygrometrica (BhDB) and Arabidopsis (AtDB1) can transport electrons across the plasma membrane using intracellular AsA as an electron donor and apoplastic monodehydroascorbic acid or Fe 3+ as electron acceptors in Xenopus laevis oocytes. Increased apoplastic AsA, autophagy and drought tolerance are observed in BhDB - and AtDB1 -overexpressing Arabidopsis compared with the wild type. CYBDOM proteins interact with respiratory burst oxidase homologue D (RbohD), which serves as the adaptor to bind autophagy related gene 8 protein (ATG8) and cargo protein for autophagic degradation. AsA increases AtDB1 protein level and its interaction with RbohD. Together, the AsA-activated CYBDOM–RbohD synergy to induce autophagy suggests a novel mechanism in plant drought and desiccation tolerance.
研究领域:
植物学
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植物生理学
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分子生物学
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应激生物学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2026
·Vol. 33
·期 7
·292
·
IF 3.5
Zhao Shen,Zhao Shicheng
The effect of poly(butyl acrylate) (PBA) rubber particle size on the mechanical and rheological properties of acrylate-styrene-acrylonitrile (ASA) resin was systematically investigated. A series of PBA latices with particle sizes ranging from 200 to 480 nm were synthesized via semi-continuous seeded emulsion polymerization, and then grafted with styrene-acrylonitrile copolymer (SAN) to form core-shell particles (PBA-g-SAN). The notched impact strength exhibited a strong particle size dependence, first increasing then decreasing, and reached an optimum of 11.6 kJ/m 2 at 395 nm—a 141.7% improvement over the 200 nm sample. This enhancement could be attributed to the optimal particle size (395 nm) effectively inducing large-scale crazing and shear yielding in the SAN matrix, as confirmed by scanning electron microscopy. Dynamic mechanical analysis verified that all samples maintained a distinct two-phase morphology, with glass transition temperatures exhibiting only marginal variations, indicating that the performance improvement arises predominantly from geometric effects rather than matrix plasticization. Rheological measurements further revealed that the 395 nm particles achieve an optimal interfacial entanglement density, ensuring efficient stress transfer and melt homogeneity while avoiding the excessively constrained network formed by smaller particles. This study establishes a clear relationship between PBA particle size control and ASA resin performance improvement, providing a mechanistic basis for designing high-performance weather-resistant ASA resins.
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高影响因子
Plants-Basel
·
2021
·Vol. 10
·期 1
·176
·
IF 3.935
Yubin Zhang,Lingyan Zha,Wenke Liu,Chengbo Zhou,Mingjie Shao,Qichang Yang
To study the effects of light quality of continuous light before harvest on the growth and ascorbic acid (AsA) metabolism of lettuce (Lactuca sativaL.) grown under relative high nitrogen level, lettuce plants grown under different nitrogen levels (8, 10 and 12 mmol·L−1) were subjected to continuous light with different red: blue light ratios (2R:1B and 4R:1B) before harvest. The results showed that the shoot fresh weight of lettuce under 12 mmol·L−1nitrogen level was significantly higher than that under other treatments. There were no significant differences in shoot dry weight, root fresh weight, root dry weight, soluble sugar content, nitrate content and AsA content in leaves among the treatments at different nitrogen levels. The content of AsA in leaves was significantly higher than that in petioles before and after continuous light. Under the same nitrogen level, the fresh weight of lettuce under continuous light quality 4R:1B was significantly higher than that under other treatments. The content of AsA in lettuce leaves increased in different degrees after continuous light before harvest. High yield and AsA content could be obtained by 72 h continuous light with red and blue light 4R:1B at 12 mmol·L−1nitrogen level. After continuous light, the content of AsA increased significantly due to the increase of the ratio of red light and nitrogen level, which increased the activities of L-galactono-1,4-lactone dehydrogenase (GalLDH) and dehydroascorbic acid reductase (DHAR) involved in AsA synthesis and in the recycling of DHAR to AsA respectively.Keywords:ascorbic acid metabolism;nitrogen levels;light quality;continuous light;LEDs
研究领域:
植物生理学
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农学
·
园艺学
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PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Wenxin Huang,Zepeng Mao,Zhiren Xu,Bo Xiang,Jun Zhang
The core-shell polymeric polyacrylate-graft-poly(acrylonitrile-ran-styrene) (ASA) has been explored for the polymer industry due to its excellent properties. However, it has been a longstanding challenge to synthesize large size-tunable particles by the conventional batch polymerization. In this paper, an accurate method, i.e. pre-emulsion semi-continuous polymerization, is applied to prepare ASA polymeric particles, which makes a series of different particle sizes (100–450 nm) of the crosslinked poly(butyl acrylate) (PBA) inner core coated with a hard shell using the poly(acrylonitrile-ran-styrene) (AS) copolymer . Specifically, the feed modes and proportion of seed are investigated to tune the particle size. The results show that the PBA seed microsphere in pre-emulsion semi-continuous polymerization is enlarged by accurate feed rate, but not in batch polymerization. A possible mechanism for the growth of the PBA core is proposed that secondary particles from homogeneous nucleation in semi-continuous feed mode is less than that of batch mode, which attenuate the impact on the growth of core particle. The size and morphology of resulting particles are characterized using various analytical techniques including the transmission electron microscopy (TEM), the scanning electron microscopy (SEM) and the dynamic light scattering (DLS). Core-shell separates phase corresponding to different glass transition temperatures is evaluated using differential scanning calorimeter (DSC) analyses indirectly. Moreover, the mechanical properties including impacts and tensile strengths are also analyzed. This study thus highlights a detailed strategy to tune polymeric particles related to different properties and the nucleating mechanism of seed emulsion polymerization that governs structure, particle size and distribution of ASA polymer with a bespoke structure for various application.
研究领域:
高分子化学
·
材料科学
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-19
·
IF 10.2
Qiu Lei,Shen Renbin,Wei Lei,Xu Shujuan,Xia Wei,Hou Yan,Cui Jinxin,Qu Rong,Luo Jiale,Cao Jian,Yang Jie,Sun Jing,Ma Ronglin,Yu Qiang
Patients with inflammatory bowel disease (IBD) always suffer from severe abdominal pain and appear to be at high risk for colorectal cancer. Recently, the co-delivery of targeted drugs and gut microbiota has developed into an attractive strategy. A new strategy using gut microbiota fermentation to overcome the interspace diffuse resistance from the mucus layer to control drug release in inflammatory bowel sites (IBS sites) has not yet been available. Here, we designed an alginate hydrogel microsphere encapsulating bifidobacterium (Bac) and drug-modified nanoscale dietary fibers (NDFs). The hydrogel microsphere is responsible for protecting drugs from acidic and multi-enzymatic environments and delivering drugs to the colorectum. Subsequently, the fermentation of Bac by digesting NDFs and proteins as carbon and nitrogen sources can promote drug release and play a probiotic role in the gut microbiota. In vitro evidence indicated that small-sized NDF (NDF-1) could significantly promote short-chain fatty acid (SCFA) expression. Notably, NDF-1 hydrogel microspheres showed a boost release of 5-ASA in the IBS sites, resulting in the amelioration of gut inflammation and remodeling of gut microbiota in chronic colitis mice. This study developed a controlled release system based on microbial fermentation for the treatment of IBD.
研究领域:
药学
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生物技术
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胃肠病学
·
微生物学
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DOI
PubMed
高影响因子
Iranian Journal of Basic Medical Sciences
·
2019
·
IF 1.854
Yan Yan,Fengling Ren,Pengchong Wang,Ying Sun,Jianfeng Xing
Objective(s): This study is aimed to design and synthesize a prodrug of 5-aminosalicylic acid and evaluate its ameliorative effect on experimental ulcerative colitis (UC). Materials and Methods: 5-Aminosalicylic acid-alanine (5-ASA-ALA) was synthesized and characterized. Its stability study was conducted in rat plasma and in the gastrointestinal tract environment, its transport characteristic was assessed using the Caco-2 cells. Its colon-targeting property was evaluated by the pharmacokinetic study, and incubation studies. A series of indicators were used to investigate its therapeutic effect on experimental colitis, including the survival rate and body weight of mice, the disease activity index (DAI), the colonic damage score and colon index, the myeloperoxidase (MPO) activity and the levels of malondialdehyde (MDA), total superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) in colonic tissues. Results: 5-ASA-ALA was barely absorbed in the Caco-2 monolayer or into the rat blood. It was remarkably stable when incubated in the upper gastrointestinal tract, while gradually hydrolyzed in the colon of rats. When orally administered to mice, 5-ASA-ALA had significantly greater therapeutic effect on colitis than the positive control. Conclusion: 5-ASA-ALA is demonstrated to be a promising oral colon-targeting prodrug of 5-ASA and has potential application in UC treatment.
研究领域:
药物学
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胃肠病学
·
药物开发
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Runqiao Chen,Shuheng Yi,Zhipan Wen,Han Feng,Xing Yin,Lingyue Shu,Yalei Zhang
To enhance the special selectivity and recognizability of two kinds of typical phenylarsonic compounds ( p -arsanilic acid ( p -ASA) and roxarsone (ROX)) in complicated water bodies, novel double-template molecularly imprinted polymers (DMIPs) were successfully synthesized via the suspension polymerization method. Subsequently, laboratory scale tests were carried out to systematically investigate the adsorption behavior and performance of p -ASA and ROX by using polymers. The result indicated that DMIPs exhibited super p -ASA/ROX sequestration and immobilization compared with non-imprinted polymers (DNIPs), which was associated with imprinting effect of polymers. The better removal efficiency of p -ASA/ROX by DMIPs were occurred in a broad pH region (3.0–9.0), whereas solution pH severely affected the uptake of DNIPs for organic arsenic compounds. According to the remarkable binding selectivity and special recognizability of DMIPs, common inorganic ions and natural organic matters (NOM) were negligible influence on targeted pollutants removal, and excellent adsorption capacity could still maintain even in the presence of interfering substances that displayed similar chemical structure with templates to a certain extent. Finally, the remarkable reusability and stable performance in continuous flow studies demonstrated the obtained DMIPs were promising candidates for simultaneously and effectively eliminating these multiple organic arsenic pollutants in harsh and complicated aquatic environment.
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DOI
高影响因子
BMC Oral Health
·
2024
·Vol. 24
·期 1
·1-10
·
IF 2.6
Li Shaoping,Jia Cancan,Han Haitong,Yang Yuqing,Xiaowen Yundeng,Chen Zhiyu
Background Polyetheretherketone (PEEK) is well known for its excellent physical–chemical properties and biosafety. The study aimed to open up a new method for clinical application of PEEK to reconstruct large-scale bone defects.Methods A bilayer scaffold for bone regeneration was prepared by combining a sulfonated PEEK barrier framework (SPEEK) with a hydrogel layer loaded with aspirin (ASA) and nano-hydroxyapatite (nHAP) by the wet-bonding of Polydopamine (PDA).Results The hydrogel was successfully adhered to the surface of SPEEK, resulting in significant changes including the introduction of bioactive groups, improved hydrophilicity, and altered surface morphology. Subsequent tests confirmed that the bilayer scaffold exhibited enhanced compression resistance and mechanical compatibility with bone compared to a single hydrogel scaffold. Additionally, the bilayer scaffold showed stable and reliable bonding properties, as well as excellent biosafety verified by cell proliferation and viability experiments using mouse embryo osteoblast precursor (MC3T3-E1) cells.Conclusion The bilayer bone regeneration scaffold prepared in this study showed promising potential in clinical application for bone regeneration.
研究领域:
生物医学工程
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材料科学
·
组织工程
·
骨科
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Hanhan Xie,Ji Yang,Tong Liu,Xinyue Zhang,Shan Zuo,Yingjie Gao,Xi Kuang,Tao Zhang
Lignin is dietary fiber from plant cell walls with multiple biological antioxidant and anti-inflammatory activities. However, whether lignin protects from ulcerative colitis (UC) and underlying mechanisms is unclear. Herein, UC mouse modeling was established with dextran sulfate sodium (DSS) and treatment with lignin for 1 week. Results showed that lignin inhibited the disease activity index (DAI), histological damage, and cell death in UC mice. We also found that lignin reversed the alterations of ferroptotic features in DSS-induced mice and ferroptotic cells, as evidenced by increased expression of GPX4 and 4-HNE and decreased transferrin expression, ameliorating the phenomenon of iron overload, GSH depletion, and ROS and MDA production. Furthermore, the increased expression of NRF2 was observed in IECs under lignin treatment. Also, the upstream regulatory molecule ERK of NRF2 was activated. Further research revealed that lignin can bind GPR37. Meanwhile, lignin was able to alleviate colitis by improving the composition of the intestinal flora in DSS mice and its effect was similar to that of 5-ASA. Taken together, these findings suggest that lignin protects against ferroptosis in UC by combining GPR37 and activating the ERK-NRF2-GPX4 signaling axis, which provides new ideas for clinical intervention in the treatment of UC.
研究领域:
胃肠病学
·
药理学
·
分子生物学
·
免疫学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）