RSC Advances
·
2019
·Vol. 9
·期 44
·25274-25284
·
IF 3.049
Xiaoqin Niu,Dan Li,Yuhong Chen,Fen Ran
Polyethersulfone (PES) has good thermal stability, superior pH, chlorine tolerance, and excellent chemical resistance; however, the hydrophilicity and biocompatibility of PES need to be improved for its real applications. In this study, we report a surface modification method for the preparation of a functional PES membrane with hydrophilic polymer chains (MPC and GMA) via surface-initiated electrochemically-mediated atom-transfer radical polymerization (SI-eATRP) technology, and the Arg–Glu–Asp–Val polypeptide groups (REDV) were immobilized onto the modified membrane by a ring-opening reaction. XPS and SEM were used to analyze the chemical composition and morphology of the modified membrane surfaces, confirming that the hydrophilic polymer chains MPC and GMA and the polypeptide group REDV were successfully grafted onto the PES membrane surface. The static water contact angle decreased from 89° to 50–65°, and the hydrophilic property of the modified membrane was enhanced. The water flux increased from 4.29 L m−2 h−1 for the pristine PES membrane to 25 L m−2 h−1 for the modified membrane with PGMA chains grafted on it and REDV functional groups immobilized on it; note that the antifouling tests showed that all the modified membranes had the higher flux recovery ratio values (FRR) of above 80% than the pristine PES membrane (about 60%), and the APTT for the modified membrane increased from 46 s to 93 s, indicating that these modified membranes could be applied in the separation and blood purification fields.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aging and Disease
·
2023
·
IF 7.4
Guoxiang Liu,Xiaoxia Li,Fanghao Yang,Jingyu Qi,Lipeng Shang,Huhu Zhang,Shuang Li,Fenghua Xu,Lingne Li,Huaxin Yu,Yang Li,Xiaolei Dong,Qinghang Song,Feng Zhu,Guang Chen,Can Cao,Liangqian Jiang,Junzhe Su,Lina Yang,Xiaohui Xu,Zhe Zhang,Robert Chunhua Zhao,Bing Li
The senescence of mesenchymal stem cells (MSCs) impairs their regenerative capacity to maintain tissue homeostasis. Numerous studies are focusing on the interventions and mechanisms to attenuate the senescence of MSCs. C-phycocyanin (C-PC) is reported to have multiple functions such as antitumor, antioxidation, anti-inflammation and anti-aging roles, but there is little research about the effects of C-PC on the senescence of MSCs. Here we investigated the roles and mechanism of C-PC on MSCs senescence. In vitro results showed that C-PC could reduce senescence, enhance proliferation, promote the adipogenic and osteogenic differentiation in senescent MSCs induced by oxidative stress. In vivo D-Galactose (D-Gal) induced rats aging models showed C-PC also increased the viability and differentiation of intrinsic senescent bone marrow derived MSCs (BMSCs). Furthermore, C-PC also decreased the levels of oxidative stress markers ROS or MDA, elevated the SOD activity, and increased the anti-inflammatory factors. Proteomic chip analysis showed that C-PC interacted with ZDHHC5, and their interaction was verified by pull down assay. Overexpression of ZDHHC5 aggravated the senescence of MSCs and greatly lessened the beneficial effects of C-PC on senescence. In addition, we found ZDHHC5 regulated autophagy by altering LC3, Beclin1 and PI3K/AKT/mTOR pathway. In summary, our data indicated that C-PC ameliorates the senescence of MSCs through zinc finger Asp-His-His-Cys (DHHC) domain-containing protein 5 (ZDHHC5) mediated autophagy via PI3K/AKT/mTOR pathway. The present study uncovered the key role of autophagy in MSCs senescence and PI3K/AKT/mTOR pathway may be a potential target for anti-senescence studies of MSCs.
研究领域:
干细胞生物学
·
衰老研究
·
分子生物学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG TARGETING
·
2021
·
IF 5.121
Chunjing Guo,Yanguo Su,Bingjie Wang,Qiang Chen,Huimin Guo,Ming Kong,Daquan Chen
With the increasing number of oncology patients and the use of chemotherapeutic agents, tumour multidrug resistance is becoming more and more prevalent. The search for new tumour treatment strategies to overcome tumour multidrug resistance is urgent. In this study, we designed GSH and ROS dual-responsive tumour-associated macrophages (TAMs)-targeted nanoparticles (NPs) for the co-delivery of the clinical first-line anti-breast cancer chemotherapy drug paclitaxel (PTX) and baicalin (Bai), which re-educates TAMs to alter their phenotype. We synthesised oligohyaluronic acid-mannose-folic acid (oHA-Man-FA, HMF) and astragalus polysaccharide-dithiodipropionic acid-paeoniflorol (APS-S-Pae, ASP), two hybrid materials that can self-assemble in water to form hybrid nanoparticles (HP-NPs) co-loaded with paclitaxel and baicalin (HP-NPs@PTX/Bai). The experimental results show that our designed hybrid nanoparticles can be specifically released in the tumour microenvironment and deliver the antitumor drug PTX as well as Bai, which reshapes the phenotype of TAMs, to the tumour site. The hybrid nanoparticles not only effectively re-educated TAMs from M2 TAM to M1 TAM, but also ameliorated the cytotoxic side effects caused by free PTX and provided better tumour suppression than free PTX and HP.
研究领域:
肿瘤学
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药学
·
纳米技术
·
免疫治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）