BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Shuang Zhao,Cong Shi,Hongyang Hu,Zhengping Li,Gang Xiao,Qiaochun Yang,Peng Sun,Linyang Cheng,Wencheng Niu,Jinshun Bi,Zhao Yue
In this paper, a portable real-time sensing device was built for Concanavalin A (Con A) and glucose detection using a smartphone. The ion-sensitive field-effect transistor (ISFET) functioning at a low working point was selected as a small-size, low-power transducer, and dextran-capped silver nanoparticles (Dex-AgNPs) served as sensitive nanoprobes on the ISFET gate. Using the affinity between Con A and carbohydrates, Con A can be captured, and thus directly detected by the ISFET/Dex-AgNPs unit; then glucose can be determined indirectly by removing Con A from the ISFET/Dex-AgNPs/Con A unit via competition with dextran . The mechanism of this competition does less harm to the sensor, allows the reusability of the sensing device, and overcomes the Debye screening of the FET device in saline solutions . Powered by a button cell, the handheld device attains excellent Con A (0.16 ng mL −1 ) and glucose (10 nM) detection limit, and can practically be used for at least 20 days.
研究领域:
生物传感器
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纳米技术
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即时检测
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Di Shao,Shuyun Bi,Rui Zhao,Xiaoyue Sun,Xu Li,Jingfu Yu
Two sensitive surface-enhanced Raman spectroscopy (SERS) analytical methods for dinitolmide and toltrazuril were developed by silver nanoparticles (AgNPs). The dinitolmide and toltrazuril were determined in 0.0565 mol L −1 and 0.0847 mol L −1 NaOH solutions respectively. The enhancement factors (EF) were 1.76 × 10 5 for dinitolmide and 1.27 × 10 5 for toltrazuril. Under optimum conditions, the methods had good linear relationship in the concentration ranges of 0.30–33.30 nmol L −1 for dinitolmide ( I SERS = 1522.52 c + 27,890.46, c :nmol L −1 , R 2 = 0.998) and 0.30–66.70 nmol L −1 for toltrazuril ( I SERS = 1334.29 c + 46,347.83, c :nmol L −1 , R 2 = 0.999). The limits of detection (LOD) (S/N = 3) were 0.915 nmol L −1 for dinitolmide and 1.03 nmol L −1 for toltrazuril. The effects of interferences on the determinations of dinitolmide and toltrazuril were studied. These SERS methods were successfully used to measure the content of dinitolmide and toltrazuril in the duck and chicken samples. The relative standard deviation (RSD) for the detection of dinitolmide and toltrazuril samples could reach 1.45 % ( n = 5). The recoveries were 95.67%–105.39% ( n = 5) and 94.79%–99.44% ( n = 5) for dinitolmide and toltrazuril system respectively.
研究领域:
分析化学
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兽医学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Ruohua Qu,Qiting Xie,Jiang Tian,Min Zhou,Fei Ge
Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo -inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine , glutamine, alanine , and aspartic acid , as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.
研究领域:
环境科学
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生物技术
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代谢组学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 1
·388-397
·
IF 4.6
Bowen Sun,Peng Li,Shuguo Yu,Xiaohui Huang,Liangjun Ma
This study has successfully prepared three kinds of surface enhanced raman scattering (SERS) substrates, namely AgNP/CuNPs/Bragg-PSi (porous silicon, PSi), AgNPs/CuNPs/PSi and AuNPs/CuNPs/Bragg-PSi by use of an anode electrochemical etching method and a dip plating method. Results show that: the AgNPs/CuNPs/Bragg-PSi substrate has optimal SERS performance and is capable of detecting the Raman spectrum (R2 = 0.9315) of a 10−5 M–10−11 M crystal violet (CV) solution. By virtue of optimizing the concentration of copper nitrate for soaking the AgNPs/CuNPs/Bragg-PSi substrate and the annealing temperature for the AgNPs/CuNPs/Bragg-PSi substrate, this study has explored the influence of different preparation conditions on the performances of the substrate. Results show that: the optimal soaking concentration of the copper nitrate solution is 0.75 M, and the AgNPs/CuNPs/Bragg-PSi substrate annealed at 180 °C shows a more uniform microscopic structure and a stronger surface-enhanced effect. Finally, the annealed AgNPs/CuNPs/Bragg-PSi substrate is capable of realizing the high-sensitivity detection (R2 = 0.9898) on diphenyl phthalate within a concentration range of 10−3 M to 10−9 M. The AgNPs/CuNPs/Bragg-PSi substrate has the advantages of easiness and convenience in preparation, low cost, high sensitivity and the like, thus providing a broad prospect for application of SERS technology in the fields of chemical analysis and biological analysis and laying a foundation for developing more sensitive and reliable detection methods.
研究领域:
材料科学
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化学分析
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生物分析
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 32
·3079-3086
·
IF 3.532
Shuang Han,Ruonan Sun,Fu Teng,Yuan Wang,Hongtao Chu,Wei Zong,Yao Chen,Zhonghui Sun
p-Cresol is a harmful phenolic substance that can cause serious effects on human health even at a low concentration in water. Therefore, the detection of p-cresol in a water environment is particularly important. In this paper, a novel zeolite imidazolate framework-67 (ZIF-67) material with regular morphology was prepared on the surface of graphene oxide doped with silver nanoparticles. The composite was modified on the glassy carbon electrode surface to increase the specific surface area, accelerate the electron transfer rate, enhance the current response and improve the performance of electrochemical sensors. Furthermore, a layer of p-cresol-molecularly imprinted polymer was prepared on the surface of the modified electrode by electropolymerization for the selective, rapid and sensitive detection of p-cresol, which greatly improved the specific recognition of p-cresol. Under optimal conditions, the prepared sensor had a good linear range of 1.0 × 10−10 M to 1.0 × 10−5 M with a detection limit as low as 5.4 × 10−11 M, and it presented excellent reproducibility, stability and selectivity. Moreover, the sensor was successfully applied for the detection of trace p-cresol in a real water environment, providing a reliable assay for sensitive, rapid and selective detection of p-cresol in complex samples.
研究领域:
环境科学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Xuemei Ma,Jiayi Yu,Lin Wei,Qian Zhao,Liyong Ren,Zhiyong Hu
Simultaneous selective determination of Fe (Ⅱ) and Fe (Ⅲ) is of great significance to the study of iron ion tracking and release of iron supplement in gastric fluid. In this paper, a composite material (N-CQDs/AgNPs/β-CD) was prepared by a one-pot method. The various characterizations confirmed the silver nanoparticles (AgNPs) grew in situ on the surface of nitrogen-doped carbon quantum dots (N-CQDs), and the β-cyclodextrin (β-CD) and AgNPs linked together by Ag–O bonds finally presented gourd-like nanoparticles on the surface of N-CQDs. Then, N-CQDs/AgNPs/β-CD modified glassy carbon electrode (GCE) was applied to detect Fe(II) and Fe(III) simultaneously. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results confirmed that N-CQDs/AgNPs/β-CD enhanced electrode performances because of the synergistic effect between N-CQDs, AgNPs and β-CD. The sensor was successfully applied for the determination by differential pulse voltammetry (DPV) of Fe(II) and Fe(III) released from four iron supplementations in simulated gastric fluid (SGF).
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Aijing Li,Xinpeng Chen,Yurong Sun,Jin Cheng,Ping Yao,Tieling Xing,Guoqiang Chen
An essential factor affecting the photocatalytic efficiency of a catalyst is the efficient separation of photogenerated electrons and holes. In the present study, a novel heterojunction material, PCN@GO/AgNPs, was synthesized with the aim of improving the photocatalytic performance. This achievement was accomplished through the establishment of a heterojunction between graphitic phase carbon nitride (PCN) and nano silver (AgNO 3 ). The PCN was prepared from melamine powder calcined at high temperature under nitrogen environment, and was modified by graphene oxide (GO) to form heterojunction with silver nanoparticles, which resulted in a significant improvement in the photocatalytic efficiency of the catalyst. Under ultraviolet（UV）excitation, the catalytic rate constant of PCN@GO/AgNPs heterojunction on RhB dye can reach 10.594 × 10 −2 min −1 . This research represents a promising strategy to increase the surface area of PCN photocatalysts through simply doping noble metal and thus improving photocatalytic performance, which contributes to understand the reaction mechanism of photocatalytic effect.
研究领域:
材料科学
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环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Jianyu Wang,Junjie Yang,Zhenyu Cui
In this study, the flat-sheet membrane of polyvinylidene fluoride (PVDF)/polystyrene-maleic anhydride (SMA) was fabricated via the thermally induced phase separation (TIPS) technique. Subsequently, a dual-functional anti-fouling hybrid separation layer was developed by co-immobilizing zwitterionic SBMA and silver nanoparticles (AgNPs) onto the polymer membrane upper surface through Michael addition reaction and in-situ dopamine generation method. An organic-inorganic hybrid layer exhibiting dual resistance to both protein fouling and microbial fouling was constructed via the "SBMA-AgNPs" synergistic modification strategy. Furthermore, the antifouling mechanism and performance of the membrane system were systematically investigated. The results demonstrated that when BSA and Escherichia coli (E. coli) were employed as organic and microbial foulant models respectively, the membrane loaded with both zwitterionic SBMA and inorganic AgNPs achieved a flux recovery ratio (FRR) of 96.8 % and antibacterial efficiency exceeding 99.9 %, significantly outperforming membranes loaded with only either AgNPs or SBMA. Meanwhile, the irreversible resistance during operation reduced to 4.5 % and the pure water flux (PWF) decay rate reduced to 20.7 %. Finally, based on the evaluation of the long-term stability and FRR of post-backwashing, the synergistic enhancement anti-fouling mechanism of "organic-inorganic" coupling was elucidated by combining the hydration layer theory of zwitterionic SBMA and the antibacterial mechanism of AgNPs. This study offers novel insights and approaches for the future advancements of water treatment membrane technology.
研究领域:
膜技术
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水处理
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材料科学
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表面改性
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Jiahui Ji,Lianggong Zhao,Xiaohua Liu,Huifang Wu,Donghan Wang,Dan Liu,Xinyue Chen,Shilan Feng
In this study, a natural extract from the plant Radix hedysari (ER) was proven to be useful for the green synthesis of silver nanoparticles (AgNPs) and ER-AgNPs in particular. Considering the complexity of plant extracts, high-pressure liquid chromatography (HPLC) was effective at confirming the components of ER, surprisingly, formononetin was found to comprise more than 85% of the total contents. Since formononetin was confirmed to be the primary component of ER, this compound was also used as the basis to synthesize AgNPs designated FMT-AgNPs that was highly pure and of high quality. The FMT-AgNPs functionalized by formononetin were optimized for further study. A simple, fast and sensitive colorimetric method detect formothion was achieved using FMT-AgNPs. A wide linear range from 0.1 μM to 10 μM was established with a low limit of detection (LOD) of 14.6 nM. Since formononetin performed multiple roles during the synthesis, it was confirmed that the mechanism of detection was based on aldol condensation , intermolecular hydrogen bonds and electrostatic attraction between formononetin and formothion. Finally, the green synthesized FMT-AgNPs were further applied in typical fruit and vegetable samples, indicating that FMT-AgNPs were potentially highly useful.
研究领域:
纳米技术
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分析化学
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药学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 17
·4155-4163
·
IF 3.637
Li Jinge,Zhao Liying,Wen Dongxiao,Li Xiaofei,Yang Huaixia,Wang Dazhong,Kong Jinming
In this work, a new method of CYFRA21-1 DNA (tDNA) detection based on electrochemically mediated atom transfer radical polymerization (e-ATRP) and surface-initiated reversible addition-fragmentation chain transfer polymerization (SI-RAFT) cascade polymerization and AgNP deposition is proposed. Firstly, the peptide nucleic acid (PNA) probe is captured on a gold electrode by Au-S bonds for specific recognition of tDNA. After hybridization, PNA/DNA strands provide high-density phosphate groups for the subsequent ATRP initiator by the identified carboxylate-Zr 4+ -phosphate chemistry. Then, a large number of monomers are successfully grafted from the DNA through the e-ATRP reaction. After that, the chain transfer agent of SI-RAFT and methacrylic acid (MAA) are connected by recognized carboxylate-Zr 4+ -carboxylate chemistry. Subsequently, through SI-RAFT, the resulting polymer introduces numerous aldehyde groups, which could deposit many AgNPs on tDNA through silver mirror reaction, causing significant amplification of the electrochemical signal. Under optimal conditions, this designed method exhibits a low detection limit of 0.487 aM. Moreover, the method enables us to detect DNA at the level of PCR-like and shows high selectivity and strong anti-interference ability in the presence of serum. It suggests that this new sensing signal amplification technology exhibits excellent potential of application in the early diagnosis of non-small cell lung cancer (NSCLC).
Electrochemical detection principle for CYFRA21-1 DNA based on e-ATRP and SI-RAFT signal amplification technology.
研究领域:
生物化学
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分析化学
·
癌症诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
研究领域:
分析化学
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环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Ke Zhao,Xinyue Ma,Meng Wang,Zhengquan Qu,Hongxia Chen,Baoshan He,Hanyu Chen,Baozhong Zhang
In this study, an ultrasensitive detection platform for tobramycin (TOB) was developed, featuring a “sandwich” structure guided by AgNCs@PDANSs and Thi-AuNCs@ZnONSs. To address the issue of large background current peak signals in tagless sensors, Thi-AuNCs@ZnONSs composites were synthesized as signal tags. Zinc oxide nanosheets (ZnONSs) served as the loading agent, and AuNCs with the electroactive molecule Thi acted as carriers. Furthermore, AgNPs@PDANSs nanocomposites, possessing excellent electrical conductivity and large specific surface areas, were prepared as substrate materials for the modified electrodes. A “sandwich” structure strategy was also introduced to enhance the accuracy of the electrochemical aptasensor. This strategy, utilizing a dual sequence for target labeling and capture, yielded higher sensitivity and simplified the sensor construction compared to methods employing a single sequence. Under optimal conditions, the detection limit for TOB was established at 1.41 pM, with a detection range of 0.05–5000 nM. The aptasensor was effectively applied in the detection of TOB in tap and lake water, demonstrating outstanding reproducibility, selectivity, and stability. These results may serve as a reference for environmental TOB detection.
研究领域:
分析化学
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环境科学
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纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）