Foods
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2026
·Vol. 15
·期 9
·1467
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IF 6
Yuqin Wang,Kexin Yu,Tongyan Shen,Kunqing Huang,Mengdie Li,Yating Wang,Jiaqi Xi,Jintian Chen,Minjie Gao,Zhitao Li
Akkermansia muciniphila, a next-generation probiotic in the human intestinal mucus layer, exhibits significant health-promoting properties. However, traditional static culture systems fail to replicate the dynamic peristaltic environment of the gastrointestinal tract, limiting understanding of its metabolic characteristics. This study employed an improved gastrointestinal bioreactor simulating intestinal peristalsis to investigateA. muciniphilagrowth dynamics and metabolomic profiles under dynamic conditions. Dynamic cultivation significantly enhanced bacterial growth. Biomass reached 1.32 ± 0.03 g/L in bovine heart infusion (BHI) medium and 2.03 ± 0.05 g/L in BHI supplemented with 2.5 g/L porcine mucin. These values represent increases of 45.05% and 123.08% relative to static BHI cultures, respectively. Dynamic conditions markedly elevated short-chain fatty acid production (acetic, propionic, isobutyric, isovaleric acids). Untargeted metabolomics identified 1463 metabolites with 1294 showing significant differential expression. Dynamic cultivation substantially altered amino acid biosynthesis, fatty acid, purine, and pyrimidine metabolism. These findings advance the understanding ofA. muciniphilaphysiology and provide insights into its metabolic characteristics under simulated intestinal conditions.
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Journal of Future Foods
·
2025
·
IF 7.2
Mingjing Zheng,Jinhuai Zuo,Huan Ouyang,Tao Hong,Zhipeng Li,Yujia Ou,Yanbing Zhu,Hui Ni,Zedong Jiang
Akkermansia muciniphila plays a key role in maintaining gut barrier integrity, but its interaction with dietary polysaccharides remains unknown. This study investigated the effects of Bangia fusco-purpurea polysaccharide (BFP) on the growth and metabolic activity of A. muciniphila . The results showed that BFP significantly enhanced bacterial growth and short-chain fatty acid production in a dose-dependent manner. Compared with A. muciniphila in a carbon-free medium, integrated proteomic and metabolomic analyses revealed significant changes at the incubation of with 2.00 g/L BFP, including 38 differentially expressed proteins and 157 metabolites. Notably, upregulation of colonization-associated proteins (e.g., MurE, Pyk, GuaB) was observed, along with elevated levels of purine-related metabolites such as guanosine monophosphate and ribose 1-phosphate. Pathway analysis indicated modulation of purine metabolism, cGMP-PKG signaling, and tryptophan metabolism, suggesting that BFP shapes the metabolic adaptation of A. muciniphila in polysaccharide-rich environments. Furthermore, BFP enhanced the expression of MUC-2 and tight junction proteins (ZO-1, Occludin, Claudin), implying the improved intestinal barrier-enhancing effect. These findings demonstrated that BFP can promote A. muciniphila colonization through metabolic reprogramming, supporting its potential application in microbiota-targeted dietary strategies.
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Food Bioscience
·
2026
·
IF 6.2
Mingjing Zheng,Jinhuai Zuo,Binhui Zhou,Tao Hong,Yuanfan Yang,Zedong Jiang,Hui Ni,Yujia Ou
The effect of ultra-high pressure (UHP)-modified Bangia fusco-purpurea polysaccharides (UBFP) on the growth and metabolic profile of Akkermansia muciniphila, as well as the lipid-regulatory effects of its fermentation products, were investigated. Compared with native BFP, UBFP treated under moderate UHP conditions (200–400 MPa) was found to more effectively promote the growth and polysaccharide utilization of A. muciniphila . In oleic acid-induced HepG2 cells, significant reductions in triglyceride levels and lipid droplet accumulation were observed after treatment with UBFP fermentation products ( p < 0.05), particularly in the UBFP 400 group, which exhibited 12.5% and 20.07% greater reductions than the BFP group, respectively. Metabolomics and proteomics analyses suggested that UBFP fermentation products may influence lipid homeostasis through alterations in lipid-related metabolites and proteins, including hypoxanthine, inosine, FABP3, PLIN4, ALB, FABP4, and SCD5. These alterations were mainly associated with AMPK/ACC-related signaling and several metabolic pathways, including purine metabolism, fatty acid metabolism, and the p53 signaling pathway. These findings provide a theoretical basis for the development of A. muciniphila -mediated algal polysaccharide functional ingredients targeting lipid metabolism.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 12
·5839-5848
·
IF 4.1
Xuan Yao,Anqi Wang,Bo Gao,Yu Xue,Xueling Zhao,Xuanyu Wang,Rui Wang,Min Zhuang,Changqing Wei,Weidong Cheng,Jian Zhang,Padraig Strappe,Zhongkai Zhou
BACKGROUND Ageing and associated cognitive impairments are becoming serious issues around the world. In this study, the physiological properties of three kinds of complexes of fatty acid (capric, stearic and oleic acid, respectively) and de-branched starch molecules were investigated via a d -galactose-induced ageing model. This study revealed differences in the regulation of cognitive impairment and brain damage following intervention of different complexes, which might highlight a potent approach for the prevention of this chronic disease. RESULTS Data indicated that three complexes improved response time and cognitive function and the bio-parameter markers associated with oxidative stress in ageing rats. Among them, the complexes prepared from de-branched starch–oleic acid showed a greater improvement compared to others. In addition, de-branched starch–capric acid complex showed a higher improvement in the morphology of colon cells and hippocampal neuronal cells. The consumption of de-branched starch–capric acid and –oleic acid complexes generated more short-chain fatty acids in the gut. More importantly, the complexation of de-branched starch with either caprate or stearate enhanced gut Akkermansia . Therefore, it was proposed that the richness in Akkermansia and gut metabolites might be associated with reduced damage of the hippocampal neuronal cells induced by the ageing progress. Moreover, the AMPK (AMP-activated protein kinase) pathway was activated in liver in de-branched starch–capric acid complex diet. In summary, de-branched starch–capric acid complex exhibited a greater effect on the attenuation of ageing-induced cognitive impairment. CONCLUSION This study might highlight a new approach for intervening in the cognitive impairment during the ageing progress via a food supply. © 2023 Society of Chemical Industry.
研究领域:
营养学
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老年医学
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神经科学
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Journal of Advanced Research
·
2026
·
IF 17.1
Yuting Zhang,Ruopeng Yin,Wang Dong,Li Sun,Tao Wang,Jingzu Sun,Huanqin Dai,Yanliang Bi,Liangliang Wang,Hongwei Liu
Introduction Sepsis is a life-threatening syndrome of organ dysfunction driven by a dysregulated immune response. Effective therapeutic strategies to restore immune homeostasis remain limited. Hypoacylated lipooligosaccharides (ALOS) from Akkermansia muciniphila have emerged as potential immunomodulatory postbiotics, yet their therapeutic potential in sepsis and the underlying mechanisms remain unexplored. Objectives This study aims to investigate whether ALOS confers protection in experimental models of sepsis induced by toxic lipopolysaccharides (LPS) or cecal ligation and puncture (CLP), and the mechanism by which ALOS exerts its anti-inflammatory and regulatory effects. Methods In LPS or CLP-induced mouse sepsis models and a porcine sepsis model, ALOS (0.2 mg/kg, i.p.) was administered once every two days before challenge or half an hour post-surgery. Survival rates, physiological and biochemical parameters were assessed. Based on 16S rRNA gene amplicon sequencing and barrier function assessment, the changes of the colonic microbiota and metabolites and anti-inflammatory capacity were analyzed. The anti-inflammatory and immunomodulatory mechanisms of ALOS were investigated through dendritic cell phenotyping, transcriptomic analysis, inhibitor assays, and conditioned medium experiments. Finally, the safety of ALOS was evaluated in C57BL/6J mice following one-week administration (0.2 mg/kg, i.p.). Results ALOS pretreatment significantly suppresses sepsis, reduces the proportion of pro-inflammatory Th17 cells, and increases regulatory T cells (Treg). Mechanistically, ALOS induced semi‑mature dendritic cells with upregulated IDO1 expression, leading to enhanced production of kynurenine (Kyn). Kyn activated the aryl hydrocarbon receptor (AhR) to drive Treg differentiation. The protective effect of ALOS was completely reversed by NLG919, whereas Kyn administration mimicked the therapeutic benefit. ALOS also produced therapeutic protection on sepsis. In a porcine sepsis model, pretreatment of ALOS exerted systemic anti‑inflammatory effects and protected multiple organs. Conclusion This study highlights the protective role of ALOS in sepsis and identifies the IDO1-Kyn-AhR immune axis as the major underlying mechanism.
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Journal of Orthopaedic Translation
·
2026
·
IF 9.8
Yimin Pi,Haodong Zhu,Jian Xie,Furui Tang,Zhuoya Wang,Li Liu,Yuchun Zhong,Zhidong He,Tian Liu,Jiacheng Zheng,Yuwen Tang,Jun Luo,Cihua Zheng
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder frequently accompanied by extraintestinal complications such as bone loss, yet effective treatment options remain elusive. In this study, we investigated whether hesperetin mitigates colitis-associated bone loss by modulating the “gut–bone axis.” Using a DSS-induced mouse model of chronic colitis, we found that hesperetin significantly reduced intestinal inflammation, restored mucosal barrier integrity, and suppressed serum inflammatory cytokines. Furthermore, hesperetin effectively alleviated DSS-induced bone microstructure damage, promoted the expression of ALP, Runx2, OCN, and inhibited NFATc1 and CTSK. Fecal microbiota transplantation experiments further confirmed that the intestinal microbiota from hesperetin-treated group attenuated intestinal inflammation and bone loss. 16S rRNA sequencing and metabolomics revealed that hesperetin enriched Akkermansia muciniphila ( A. muciniphila ) and increased the tryptophan metabolite 3-indoleacrylic acid (IDA). Supplementation with A. muciniphila or IDA alone simulated the therapeutic benefits of hesperetin. Furthermore, RNA-seq revealed that hesperetin regulated bone metabolism-related gene expression by ECM-receptor interaction and PI3K-AKT signaling, while inhibiting the NOD-like receptor pathway. In conclusion, hesperetin mitigates intestinal inflammation and bone homeostasis imbalance by reshaping the gut microbiota, enriching A. muciniphila, and promoting IDA production, thus providing new insights into potential therapies for IBD-related bone loss. Download: Download high-res image (369KB) Download: Download full-size image
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Probiotics and Antimicrobial Proteins
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2025
·
IF 4.4
Ma Xue-Ke,Shao Xin,Xu Tian,Yan Hong,Teng Hui,Chen Zhe-Xin,Hu Meng-Xin
Astaxanthin (ASX) gains wide interest in food and nutritional sciences due to its great antioxidant and anti-inflammatory properties. However, ASX faces challenges such as poor water solubility, low bioavailability, and sensitivity to environmental factors. In this study, we employ Akkermansia muciniphila ( AKK ) with mucoadhesive property as a carrier for ASX to enhance ASX stability and bioaccessibility. The complex multilayered cell envelope of AKK cell provided space for ASX loading. Immersion loading yielded the highest ASX encapsulation efficiency (10.80 ± 2.29 μg/mg in ASX@ AKK group and 11.47 ± 3.52 μg/mg in ASX@ AKK -Heat group), whereas osmoporation loading and vacuum loading yielded ASX encapsulation efficiencies of 4–6 μg/mg. The encapsulated ASX demonstrated improved thermostability, tolerating pasteurization, and was predominantly amorphous in state, interacting with AKK primarily through hydrophobic interactions and partial hydrogen bonding interactions. The ASX@ AKK system facilitated precise ASX release in the intestine and markedly enhanced bioaccessibility, increasing approximately 25.7-fold in simulated intestinal fluid and 77.6-fold in simulated colonic fluid. Importantly, ASX encapsulation did not impair AKK ’s adhesive affinity to mucus. Therefore, the newly developed integrated dietary supplements have both the functional activity of inactivated AKK as a postbiotic and the antioxidant property of the intestine-targeted released ASX, showing dual-functional activities. These findings highlight a paradigm shift in the functional application of probiotics, showcasing their potential as innovative vehicles for targeted nutrient delivery with improved bioaccessibility and synergistic health effects.
研究领域:
食品科学
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营养学
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微生物学
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Food & Function
·
2026
·
IF 6.3
Ziyuan Zhou,Xiaxia Pan,Jiali Ni,Zhuoqi Lou,Yangfan Shen,Shiman Jiang,Xiangmin Dong,Fengjiao Wang,Yanfei Chen,Lanjuan Li
Existing evidence indicates that pre-existing metabolic dysfunction-associated steatotic liver disease (MASLD) confers susceptibility to acute liver injury (ALI). Nevertheless, the underlying mechanism prompting ALI risk in such condition is not fully uncovered yet. Gut dysbiosis has been well-documented in MASLD patients which prompts us to hypothesize that gut dysbiosis may serve as a contributing factor and regulating target to ALI susceptibility. A case-control study was performed and verified the increased risk of ALI in MASLD patients. Mice pretreated with MASLD mice/patients-derived fecal microbiota transplantation (FMT), demonstrated worsened ALI after D-GalN/LPS injection. We identified significant gut dysbiosis with decreased microbial α-diversity, beneficial genus lactobacillus, increased potential harmful bacteria Campylobacter and helicobacter. Probiotic Akkermansia muciniphila (A. muciniphila) was reported to improve gut dysbiosis. Thus, we tested the effect of A. muciniphila on ALI mice pretreated with MASLD mice-derived FMT. A. muciniphila significantly ameliorated the worsened ALI and improved gut dysbiosis. Transcriptomic analysis reveled that A. muciniphila restored the impaired liver PPAR-α signaling, alleviating the aggravated NF-κB signaling and downstream inflammation response in liver. Specifically, A. muciniphila restored the decreased abundance of PPAR-α agonist daidzein in colon, thereby restoring PPAR-α activation and inhibiting NF-κB signaling in liver. Daidzein is also capable to ameliorate ALI aggravated by MASLD-associated dysbiosis, accompanied by PPAR-α activation and NF-κB inhibition. Our findings revealed that gut dysbiosis in MASLD increased the risk of ALI. A. muciniphila exhibited preventive potential for MASLD patients to reduce ALI risk. Daidzein/PPAR-α/NF-κB signaling is important to maintain host resistance to hepatoxic challenge.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Kangle Li,Hongjie Cui,Si Wang,Qianfu Gan,Qinghua Liu,Shuangbo Huang
Alterations in gut microbiota and its metabolites are associated with placental and fetal development. Although proline has been shown to enhance placental function and improve pregnancy outcomes, its interaction with the gut microbiota remains poorly understood. In this study, a gestational low-protein diet (LPD) leads to decreased fetal birth weight and impaired placental vascular development, as well as increased embryonic resorption rates; these effects are mitigated by dietary proline supplementation. Mechanistically, proline enhances the abundance of Akkermansia and the production of butyrate in the gut, thereby promoting placental angiogenesis by activating the placental Wnt/insulin-like growth factor 1 (Igf1) signaling pathway, ultimately alleviating fetal developmental impairment caused by LPD. Moreover, in an in vitro porcine vascular endothelial cell model under amino acid-restricted conditions, butyrate enhances angiogenesis by activating the Wnt/Igf1 signaling pathway. These findings reveal that targeting the dietary proline supply and gut microbiota may open new avenues for the prevention of intrauterine growth restriction.
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Journal of Environmental Sciences
·
2025
·
IF 6.3
Gang Wei,Wangrui Liu,Fengjie Shen,Rongrong Xie,Fengwei Wang,Huaqi Guo,Lin Liu
The global rise in intestinal dysfunction has been linked to modifiable risk factors including high-fat diet (HFD)-induced obesity / diabetes, microplastics (MPs) and aging, yet their combined effects remain unclear. This study examined the combined effects of chronic low dose polystyrene MPs (∼25 μg/(kg·day)) with HFD or aging on intestinal barrier function in C57BL/6J mice over 14 weeks. Juvenile mice receiving either normal chow (NCD) or HFD, along with aged NCD-fed mice, were subjected to MPs exposure. Results demonstrated that MPs exposure or HFD feeding (and aging) significantly exacerbated the pathological changes developed in colon tissues of mice relative to those of control mice, including gut permeability, oxidative stress, pro-inflammatory response and apoptosis. Moreover, combination of MPs exposure with HFD feeding (and aging) further potentiated the harmful effects on colon dysfunctions, clearly showing a synergistic deterioration effect (‘double hit’). More importantly, we found that the recovery of colon damages resulting from MPs exposure and HFD feeding was disrupted after their withdrawal, leading to the worse effects on gut microbiota dysbiosis and intestinal function. Furthermore, oral administration of Akkermansia muciniphila prevented intestinal barrier dysfunction and decreased intestinal permeability from MPs exposure and HFD feeding co-exposure. Our findings in this study emphasized the important role of avoiding MPs exposure in the prevention and treatment of intestinal-related dysfunctions, and provided potential therapeutic approaches. The oral administration of gut microbiota, such as Akkermansia muciniphila , contributed to restoration of intestinal barrier function, uncovering a new landscape for treating intestinal disabilities caused by MPs exposure.
研究领域:
环境科学
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医学
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微生物学
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Food Science & Nutrition
·
2024
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IF 3.5
Xi Chen,Yong Zhu,Muhammad Mazhar,Likang Qin
Camellia seed oil (CSO), a potential prebiotic agent, can significantly increase the relative abundance of Akkermansia muciniphila ( A. muciniphila ) in mice gut microbiota following oral administration, this study aims to investigate the enhancing effect in vitro. The results showed that after 24-h co-cultivation with 0.5% (v/v) CSO, the growth of A. muciniphila increased from 11.61 ± 0.04 Log 10 CFU/mL to 12.17 ± 0.10 Log 10 CFU/mL ( p < 0.05), accompanied by a reduction in the oxidation–reduction potential (ORP) value of the media from −126.67 ± 1.78 mV to −117.33 ± 0.72 mV ( p < 0.05). Additionally, squalene and (+)-α-tocopherol, bioactive compounds present in CSO, were found to promote A. muciniphila proliferation (squalene OD 600 : 1.086 ± 0.002, tocopherol OD 600 : 1.100 ± 0.003, DMSO control OD 600 : 0.991 ± 0.003, p < 0.0001). Transcriptomic and metabolomic profiling revealed 464, 121, and 194 differentially expressed genes (DEGs) and 212, 160, and 156 differentially expressed metabolites (DEMs) in A. muciniphila co-cultivated with CSO after 4, 16, and 24 h, respectively ( p < 0.05). The upregulated DEGs and DEMs were primarily enriched in pathways associated with energy generation (e.g., gap , icd , sucC , GOZ73_RS04175 , succinate, phosphoenolpyruvate), nucleotide metabolism (e.g., mazG, deoxyguanosine), amino acid metabolism (e.g., argF , metK, L-tyrosine), translation (e.g., rplO , rpmC ), and environmental adaptation (e.g., murA , katE , reduced nicotinamide adenine dinucleotide). These findings suggest that various bioactive compounds present in CSO exhibit prebiotic effects on the in vitro proliferation of A. muciniphila by facilitating nutrient utilization and environmental adaptation. This study provides insights into the extended utilization of CSO.
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Advanced Science
·
2026
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IF 14.1
Kaiyuan Jiang,Fen Xiong,Yiwen Peng,Lingfei Meng,Xinran Wang,Yingruo Xu,Tian Tang,Hongchang Gao
Diabetic cardiomyopathy (DCM) is a major cardiovascular complication of diabetes with limited effective interventions. Using a streptozotocin-induced insulin-deficient, type 1 diabetes-like DCM mouse model, we show that intermittent fasting (IF) improves cardiac function and attenuates myocardial remodeling. Antibiotic-mediated microbiota depletion largely abolished these benefits, whereas fecal microbiota transplantation from IF-treated donors recapitulated cardioprotection, supporting a causal role of the gut microbiota. Metagenomic profiling identified Akkermansia muciniphila ( A. muciniphila ) as a prominent IF-responsive taxon, and A. muciniphila supplementation alleviated cardiac injury without obvious improvement in glycaemia. Integrated serum and heart metabolomics identified 1-methyl-L-histidine as a microbiota-associated metabolite reduced in diabetes but restored by IF and A. muciniphila . In vitro and ex vivo assays further supported an L-anserine-linked microbial route for 1-methyl-L-histidine generation. Importantly, oral 1-methyl-L-histidine supplementation recapitulated key cardioprotective effects, remodeled cardiac lipid homeostasis, and reduced lipid peroxidation and oxidative injury. Together, these findings support a gut microbiota–metabolite–lipid axis associated with IF-related cardioprotection in DCM and highlight microbial metabolites as tractable targets to complement dietary intervention.
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