Marine Drugs
·
2026
·Vol. 24
·期 4
·125
·
IF 5.7
Zi-Han Xu,Zheng-Biao Zou,Chun-Xiu Wang,Chen Li,Xian-Wen Yang,Jun-Song Wang
Inhibition of inflammation and oxidative stress is increasingly recognized as a promising therapeutic strategy for neurodegenerative diseases. In this study, we isolated two new dimeric naphtho-γ-pyrone (aS)-fonsecinones B and D (1and2) and 14 known compounds (3–16) from the deep-sea-derived fungusAspergillus niger3A00562. Their structures were unambiguously determined through integrated physicochemical and spectroscopic analyses. Screening for neuroinflammatory inhibitors using a BV2 microglial cell model identified TMC 256 A1 (10) as the most potent candidate. Compound10significantly suppressed LPS-induced inflammation in BV2 cells without cytotoxicity. It concurrently inhibited LPS-triggered ROS overproduction and neutrophilic infiltration in zebrafish. Subsequent proteomics revealed that10targets NOS2 to modulate Alzheimer’s disease (AD)-associated pathways and the KEAP1-NRF2 axis. Molecular docking and dynamics simulations demonstrated that10occupies the NOS2 heme-binding pocket, thereby preventing dimerization and inhibiting enzymatic activity. Finally,10ameliorated locomotor deficits in an AD zebrafish model. Collectively, these findings highlight compound10as a candidate compound for preventing inflammatory and oxidative stress damage during treatment of neurodegenerative diseases, particularly AD.
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高影响因子
Journal of Environmental Sciences
·
2010
·
IF 1.412
Shuyan Zang,Bin Lian,Juan Wang,Yanjie Yang
To explore biodegradation of 2-naphthol and its metabolites accumulated in wastewater treatment, a series of bio-degradation experiments were conducted. Two main metabolites of 2-naphthol, 1,2-naphthalene-diol and 1,2-naphthoquinone, were identified by high-performance liquid chromatography with standards. Combining fungus Aspergillus niger with bacterium Bacillus subtilis in the treatment enhanced 2-naphthol degradation efficiency, lowered the accumulation of the two toxic metabolites. There were two main phases during the degradation process by the kinetic analysis: 2-naphthol was first partly degraded by the fungus, producing labile and easily accumulated metabolites, and then the metabolites were mainly degraded by the bacterium, attested by the degradation processes of 1,2-naphthalene-diol and 1,2-naphthoquinone as sole source of carbon and energy. Sodium succinate, as a co-metabolic substrate, was the most suitable compound for the continuous degradation. The optimum concentration of 2-naphthol was 50 mg/L. The overall 2-naphthol degradation rate was 92%, and the COD Cr removal rate was 80% on day 10. These results indicated that high degradation rate of 2-naphthol should not be considered as the sole desirable criterion for the bioremediation of 2-naphthol-contaminated soils/wastewater.
研究领域:
环境科学
·
微生物学
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PubMed
高影响因子
Journal of Biobased Materials and Bioenergy
·
2017
·Vol. 11
·期 3
·210-215
·
IF 0.975
Wang Qing-Yun,Lin Qin-Lu,Peng Kuan,Cao Jian-Zhong,Yang Chun,Xu Dong
An antifungal Bacillus strain isolated from the traditional fermented food natto was identified and named as B. subtilis natto CSUF5. Antifungal metabolites of the strain were extracted from the medium of the inhibition zone on the dual culture plate. 2 V7-surfactin
isoforms (C14–V7, C13–Emet
1-V7) and 3 I/L7-surfactin isoforms (C13–I/L7, C13,14–Emet
1–I/L7 were identified from the RP-HPLC
isolations by MS
n
(n = 1,2,3) analysis. Both V7-surfactins and I/L7 -surfactins showed slight antifungal activity against A. niger, and their MIC50 decreases in the order V7 > I/L
7. EDTA was found
to be an effective strengthening agent which could cooperate with the surfactin variants and enhance their antifungal activity greatly. Microscopic observations revealed that the surfactin variants could delay the fungal spore germination and inhibit the hyphae growth, mainly displayed in
hyphal shriveling and distortion. Surfactin variants and EDTA together could lead the hyphae to severe helical warping and fracture. These results will provide promising references for the development of new antifungal products.
研究领域:
微生物学
·
应用微生物学
·
天然产物化学
·
抗真菌剂
·
发酵食品
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Bin Jiang,Hongshen Yue,Xinhao Fu,Jiaming Wang,Yu Feng,Dongmei Li,Chunhong Liu,Zhibiao Feng
Prolyl endopeptidase from Aspergillus niger (An-PEP) is an enzyme that recognizes C-terminal peptide bonds of amino acid chains and cleaves them by hydrolysis . An aqueous two-phase system (ATPS) was used to separate An-PEP from fermentation broth. Through single factor experiments, the ATPS containing 16 % ( w/w ) PEG2000 and 15 % ( w/w ) (NH 4 ) 2 SO 4 at pH 6.0 obtained the recovery of 79.74 ± 0.16 % and the purification coefficient of 7.64 ± 0.08. It was then used to produce soy protein isolate peptide (SPIP) by hydrolysis of soy protein isolate (SPI), and SPIP-Ferrous chelate (SPIP-Fe) was prepared with SPIP and Fe 2+ . The chelation conditions were optimized by RSM , as the chelation time was 30 min, chelation temperature was 25 °C, SPIP mass to VC mass was two to one and pH was 6.0. The obtained chelation rate was 82.56 ± 2.30 %. The change in the structures and functional features of SPIP before and after chelation were investigated. The FTIR and UV–Vis results indicated that the chelation of Fe 2+ and SPIP depended mainly on the formation of amide bonds. The fluorescence, SEM and amino acid composition analysis results indicated that Fe 2+ could induce and stabilize the surface conformation and change the amino acid distribution on the surfaces of SPIP. The chelation of SPIP and Fe 2+ resulted in the enhancement of radical scavenging activities and ACE inhibitory activities. This work provided a new perspective for the further development of peptide-Fe chelates for iron supplement.
研究领域:
生物化学
·
食品科学
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PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2018
·Vol. 319
·期 3
·869-880
·
IF 1.181
Ding Hanlin,Luo Xuegang,Zhang Xiaonuo,Yang Hao
In this work, a modified biomass material was successfully prepared by immobilization and inactivation method based on Aspergillus niger , and the obtained environmentally friendly Alginate-immobilized Aspergillus niger microsphere (AAM) exhibited a superior Th(IV) biosorption performance. The sorption kinetics and thermodynamic analysis were studied and showed that the sorption process is spontaneous and endothermic under the experimental conditions. Additionally, the maximum biosorption capacity of AAMs for Th(IV) can be of 303.95 mg g −1 at 313.15 K, which exhibited a superior biosorption capacity than the most reported other biomass materials. The results illustrated the biomaterial had good recyclability through immobilization and inactivation.
研究领域:
环境科学
·
微生物学
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高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3420
·
IF 5.2
Minyu Cai,Liyan Huang,Sashuang Dong,Nanxin Diao,Weilian Ye,Zhiye Peng,Xiang Fang
Summer green tea (SGT) has a low cost and high annual yield, but its utilization rate is limited due to suboptimal quality. The aim of this study is to enhance the flavor of SGT using fermentation withA. nigerRAF106 while examining changes in its metabolites during this process. The results revealed an elevation in the content of alcohol, alkanes, and nitroxides in tea leaves following the process of fermentation. The predominant volatile compounds identified in tea leaves after undergoing a 6-day fermentation period were linalool, (Z)-α, α, 5-trimethyl-5-vinyltetrahydrofuran-2-methanol, (E)-linalool oxide (furan type), linalool oxide (pyran type), and theapyrrole. These compounds exhibited significant increases of 31.48%, 230.43%, 225.12%, 70.71%, and 521.62%, respectively, compared to the non-fermented control group (CK). The content of non-ester catechins, soluble sugars, and total flavonoids reached their peak on the 4th day of fermentation, exhibiting significant increases of 114.8%, 95.59%, and 54.70%, respectively. The content of gallic acid and free amino acids reached their peak on the 6th day of fermentation, exhibiting significant increases of 3775% and 18.18%, respectively. However, the content of ester catechin decreased by 81.23%, while caffeine decreased by 7.46%. The content of lactic acid, acetic acid, and citric acid in tea after fermentation was 421.03%, 203.13%, and 544.39% higher than before fermentation, respectively. The present study offers a fresh approach for the advancement of SGT.Keywords:summer green tea;Aspergillus niger;fermentation;flavor
研究领域:
食品科学
·
微生物学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Hui-Juan Cheng,Zhi-Wen Xie,Xin Wang,Shuo Wang,Wei Ye,Yu-Cai Lü,Da-Chun Gong,Xiao-Tao Shi,Dong-Xu Ma
β-Glucosidase (BGL), a pivotal enzyme in lignocellulosic saccharification, has been increasingly recognized as an “emerging green biocatalyst” in modern biorefinery processes. Here, Aspergillus niger An-BGL was rationally engineered to achieve high-level production of BGL under low-cost inducers. Corncob powder served as a cost-effective alternative inducer, enhancing BGL production to 14.2 U/mL. The knockout of CreA and the overexpression of Xln R increased the BGL yield by 75% and 27%, respectively. Combinatorial engineering of CreA and Xln R generated the XO–CK strain, which exhibited a derepression effect at high glucose concentrations. Supplementation with 1% glucose alleviated the delayed enzyme production in the engineered XO–CK strain, resulting in a BGL activity of 31.54 U/mL. Furthermore, integration of the bglA gene into the high-expression amyA site enhanced BGL to 40.68 U/mL. The rational modification strategy for A. niger strain established in this study offers an efficient and sustainable approach for transforming corncob agricultural waste into high-value enzymatic preparations.
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高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2016
·
IF 1.207
Tao Lu,Qilei Zhang,Shanjing Yao
In order to improve the efficient decolorization of dye-containing water by biosorbent and understand the biosorption mechanism, the self-immobilization mycelial pellets were prepared using a marine-derived fungus Aspergillus niger ZJUBE-1, and an azo dye, Congo red was chosen as a model dye to investigate batch decolorization efficiency by pellets. The pellets as biosorbent showed strong salt and acid tolerance in biosorption process. The results for dye adsorption showed that the biosorption process fitted well with models of pseudo-second-order kinetic and Langmuir isotherm, with a maximum adsorption capacity of 263.2 mg·g − 1 mycelium. During 6 batches of continuous decolorization operation, the mycelial pellets could possess efficient decolorization abilities (> 98.5%). The appearance of new peak in the UV–Vis spectral result indicated that the decolorization process may also contain biodegradation. The mechanism studies showed that efficient biosorption ability of pellets only relies on the active zone on the surface of the pellet, which can be enhanced by nutrition supplement or be shifted outward by a re-culture process.
研究领域:
环境科学
·
微生物学
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高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Tian-Lin Wu,Yong-Mei Hu,Yu Sun,Zhi-Jun Zhang,Zheng-Rong Wu,Wen-Bin Zhao,Chen Tang,Sha-Sha Du,Ying-Hui He,Yue Ma,Cheng-Jie Yang,Ying-Qian Liu
Isothiocyanates had attracted attention for many years due to their antimicrobial properties. In this study, the antifungal efficiency of eighteen isothiocyanate compounds were evaluated against six plant pathogenic fungi and two molds in vitro . Among them, 2-(4-methoxyphenyl)ethyl isothiocyanate displayed the highest antifungal activity against Aspergillus niger (EC 50 = 4.19 μg/mL). The further studies of its mode of action showed that it may damage the cell morphology and membrane integrity in a dose dependent manner by morphological observation, propyl iodide staining and the release of cellular contents. On the other hand, the decrease of related enzyme activities in tricarboxylic acid cycle suggested that 2-(4-methoxyphenyl)ethyl isothiocyanate may effected energy metabolism. Besides, reactive oxygen species, the content of malondialdehyde, the total antioxidant capacity , the content of glutathione and the kinase of oxidative reactive were changed in fungi treated with 2-(4-methoxyphenyl)ethyl isothiocyanate, compared with those in untreated fungi, which all indicated that 2-(4-methoxyphenyl)ethyl isothiocyanate exhibited antifungal activity against A.niger by inducing oxidative stress.
研究领域:
微生物学
·
药理学
·
植物病理学
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziyue Chen,Qi Lin,Cheng Peng,Kunpeng Yu,Yanling Lin,Shuyi Weng,Lijun Li,Hui Ni,Feng Chen
Tea aroma is largely shaped by volatile compounds released from glycosidic aroma precursors. This study investigated the effects of two Aspergillus niger β-glucosidases, An-G3987 and An-G8816, on modulating the aroma profile of instant Oolong tea powder. Quantitative descriptive analysis (QDA), gas chromatography–mass spectrometry (GC–MS), and odor activity value (OAV) assessments revealed that An-G3987 significantly enhanced floral, sweet, and grassy aromas by releasing key volatile compounds such as benzyl alcohol, (Z)-3-hexen-1-ol, and 1-hexanol. In contrast, An-G8816 generated a broader spectrum of volatile compounds but had a less pronounced impact on overall aroma enhancement. Kinetic studies and molecular docking analyses demonstrated that An-G3987, compared to An-G8816, exhibited higher catalytic efficiency and stronger binding affinity for typical glycosidic aroma precursors, driven by hydrogen bonding networks (e.g., Arg107/Tyr254) and hydrophobic interactions (e.g., Pro428/Tyr497). These findings indicate that An-G3987 is better suited for targeted aroma intensification, while An-G8816 may be preferable for balanced flavor modulation.
研究领域:
食品科学
·
酶学
·
风味化学
·
生物技术
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PubMed
高影响因子
Toxins
·
2014
·Vol. 6
·期 11
·3157-3172
·
IF 2.48
Wei Zhang,Beibei Xue,Mengmeng Li,Yang Mu,Zhihui Chen,Jianping Li,Anshan Shan
Aflatoxin B1, a type of highly toxic mycotoxin produced by some species belonging to theAspergillusgenus, such asAspergillus flavusandAspergillus parasiticus, is widely distributed in feed matrices. Here, coumarin was used as the sole carbon source to screen microorganism strains that were isolated from types of feed ingredients. Only one isolate (ND-1) was able to degrade aflatoxin B1after screening. ND-1 isolate, identified as a strain ofAspergillus nigerusing phylogenetic analysis on the basis of 18S rDNA, could remove 26.3% of aflatoxin B1after 48 h of fermentation in nutrient broth (NB). Optimization of fermentation conditions for aflatoxin B1degradation by selectedAspergillus nigerwas also performed. These results showed that 58.2% of aflatoxin B1was degraded after 24 h of culture under the optimal fermentation conditions. The aflatoxin B1degradation activity ofAspergillus nigersupernatant was significantly stronger than cells and cell extracts. Furthermore, effects of temperature, heat treatment, pH, and metal ions on aflatoxin B1degradation by the supernatant were examined. Results indicated that aflatoxin B1degradation ofAspergillus nigeris enzymatic and this process occurs in the extracellular environment.Keywords:Aspergillus niger;coumarin;aflatoxin B1;degradation
研究领域:
微生物学
·
毒理学
·
食品科学
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PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 8
·3453-3467
·
IF 3.094
Ye Hangyu,Li Xiaojun,Li Luyuan,Zhang Yinjun,Zheng Jianyong
Aspergillus niger has been used for homologous and heterologous expressions of many protein products. In this study, the α-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was homologously expressed in A. niger 3.350 by Agrobacterium tumefaciens -mediated transformation. The enzyme activity of Rha-N1 was 0.658 U/mL, which was obtained by cultivation of engineered A. niger in a 5-L bioreactor. Rha-N1 was purified by affinity chromatography and characterized. The optimum temperature and optimum pH for Rha-N1 were 60 °C and 4.5, respectively. Enzyme activity was promoted by Al 3+ , Li + , Mg 2+ , and Ba 2+ and was inhibited by Mn 2+ , Fe 3+ , Ca 2+ , Cu 2+ , and organic solvents. The result indicated that rutin was the most suitable substrate for Rha-N1 by comparison with the other two flavonoid substrates hesperidin and naringin. The transformed products of isoquercitrin, hesperetin-7-O-glucoside, and prunin were identified by LC–MS and 1 H-NMR.
研究领域:
生物化学
·
分子生物学
·
生物技术
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PubMed
高影响因子
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