Analytical Methods
·
2023
·Vol. 15
·期 6
·837-842
·
IF 3.1
Xiangying Sun,Xuefeng Zhang,Fang Li
AuNCs with chemical groups such as –NH2 and –COOH were synthesized using glutathione as the stabilizer and reducing agent. The aggregation emission of AuNCs in solution-induced self-assembled multilayers (SAMs) were first studied. Scanning electron microscopy and quartz crystal microbalance were used to characterize the morphology and aggregation process of AuNCs. Further AuNC SAMs were used for the solid–liquid interface sensing of water content in ethanol, and the sensitivity is obviously improved as compared with that in the pure solution phase. This aggregation emission induced by SAMs would have a good application prospect in analysis.
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高影响因子
ANALYST
·
2021
·Vol. 146
·期 16
·5115-5123
·
IF 4.616
Haiyang Yao,Difei Jiang,Gaoqiu Dong,Jiamin Sun,Shasha Sun,Lingling Li,Fenfen Zheng,Weiwei Xiong
Dynamically monitoring intracellular glutathione (GSH), a crucial biomarker of oxidative stress, is of significance for the diagnosis and treatment of certain diseases. Although manganese dioxide (MnO2) based GSH fluorescent sensors have exhibited high sensitivity and good selectivity owing to the specific reactivity between GSH and MnO2, near-infrared (NIR) MnO2 based nanoprobes for GSH detection are scarce. Herein, we have developed a NIR activatable fluorescence nanoprobe for the imaging and determination of intracellular GSH based on a core–shell nanoparticle, consisting of NIR emitted gold nanocluster doped silica as the fluorescent core and manganese dioxide as the GSH-responsive shell (named AuNCs@MnO2). Due to the absorption competition mechanism, the outer MnO2 shell rather than the inner AuNCs core preferentially absorbed the excitation light, thus leading to fluorescence quenching of the inner AuNCs core. Upon addition of GSH, the fluorescence of the nanoprobe restored along with the reduction of MnO2 to Mn2+ because of the absorption competition disappearance-induced emission. The activatable fluorescence linearly increased upon changing the GSH concentration in the range of 2 to 5000 μM with a detection limit of 0.67 μM. The cytotoxicity test shows that the AuNCs@MnO2 nanoprobes have a good biocompatibility. After entering the cancer cells, the intracellular GSH degraded the outermost MnO2 shell and initiated the NIR fluorescence restoration of AuNCs, which can be used to monitor the dynamic change of intracellular GSH. This strategy provides an NIR-activatable way to detect GSH levels in living cells and offers a promising platform for the diagnosis and treatment of GSH-related diseases.
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TALANTA
·
2023
·
IF 6.1
Pengfei Ma,Hualin Guo,Ke Li,Yin Zhang,Huiqing Guo,Zhouping Wang
Patulin (PAT) and ochratoxin A (OTA) are the two main mycotoxins present in apples. Herein, a sensitive aptasensor for simultaneous detection of PAT and ochratoxin OTA was developed. Dual-color gold nanoclusters (AuNCs) with enhanced fluorescence properties were synthesized and employed as fluorescence amplifiers. Two separated fluorescence peaks at 650 nm and 530 nm were monitored simultaneously by employing single excitation (405 nm), corresponding to the aptamer probes of Cys@BSA-AuNCs-Apt PAT and Arg@ATT-AuNCs-Apt OTA , respectively. The fluorescent aptasensor demonstrated satisfying specificity, storage ability and accuracy. Under the optimal experimental conditions, the linear detection range for PAT and OTA was 0.10–50 ng/mL, with the limit of detection of 0.09 ng/mL and 0.06 ng/mL, respectively. Most importantly, practicability of the constructed aptasensor were confirmed by conducting the determination of PAT and OTA in apple juice sample, indicating the great potential of the aptasensor in practical detection applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Lingyang Zhang,Yiying Wei,Yu Huang,Xiaoyu Huang,Gaofeng Liang,Gankun Yuan,Zhaoyi Wei,Kun Hou,Jiaheng Yang,Yanfei Wei,Lina Geng,Shenna Chen
As one of the most indispensable microelements in living organisms, the quantitative detection of iodine is of great significance in the fields of food and life sciences. Herein, a novel ratiometric fluorescent probe His/GSH-AuNCs was developed for the detection of iodide ions. The His/GSH-AuNCs, which can be readily obtained by mixing histidine-stabilized gold nanoclusters (His-AuNCs) and glutathione-stabilized gold nanoclusters (GSH-AuNCs) in an appropriate proportion, have two distinct intrinsic emission peaks at 470 nm and 620 nm when excited at 340 nm. Significantly, the His/GSH-AuNCs could precisely and sensitively capture I − in complex environments via its “in-situ” growth stimulated by iodine. Meanwhile, the fluorescence intensity of two emission peaks of His/GSH-AuNCs was hindered to varying degrees. Based on the different variations of these two emission peaks, a ratiometric fluorescence method was established with the relationship between the variation of the ratio of two emission peaks ((I 470 /I 620 )/(I 470 /I 620 ) 0 ) and the I − concentration. Furthermore, the detection mechanism was investigated by employing a combination of UV–vis and TEM techniques. Moreover, the His/GSH-AuNCs probe has been successfully applied to monitor iodide ions in environmental water samples, tap water samples, and urine samples.
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TALANTA
·
2024
·
IF 5.6
Li Zhang,Xiaoya Bi,Hui Wang,Libo Li,Tianyan You
Ligand-protected gold nanoclusters (AuNCs) have become promising nanomaterials in fluorescence (FL) methods for mercury ions (Hg 2+ ) monitoring, but low FL efficiency hinders their widespread application. Herein, AuNCs/cerium-based metal-organic frameworks (AuNCs/Ce-MOFs) were prepared by loading 6-aza-2-thiothymine-protected AuNCs (ATT-AuNCs) with aggregation-induced emission (AIE) effect on the surface of Ce-MOFs by electrostatic attraction. This strategy improved the FL intensity of AuNCs through two aspects: (i) the AIE effect of ATT-AuNCs and (ii) the confinement effect of Ce-MOFs, which improved the restriction of intramolecular motion (RIM) of ATT-AuNCs. In addition, Ce-MOFs could adsorb and aggregate Hg 2+ during detection, which might increase the local concentration. Therefore, based on the high FL signal of AuNCs/Ce-MOFs and enriched Hg 2+ , sensitive detection of Hg 2+ could be achieved. More importantly, the strong specific recognition between AuNCs and Hg 2+ could guarantee selectivity. The developed FL sensor exhibited superior detection performances with a wide linear range of 0.2–500 ng mL −1 and a low detection limit of 0.067 ng mL −1 . Furthermore, the FL sensor used for sensitive and selective detection of Hg 2+ in real samples, and the results agreed well with the standard method. In summary, this work proposed an effective and generalized strategy for improving the FL efficiency of AuNCs, which would greatly facilitate their application in pollutant monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yue Cai,Ziyin Qiu,Xubin Lin,Wei Zeng,Yiran Cao,Weipeng Liu,Yingju Liu
Organophosphorus pesticides (OPs) are widely used in agriculture. However, the residue and accumulation of OPs is a threat to food safety and human health. Thus, a sensitive and accurate OPs detection method is still pursued. Here, we utilized electrostatic adsorption to immobilize weak fluorescent AuNCs on the SiO 2 surface, exciting its aggregation-induced emission (AIE) properties and achieving the amplification of the fluorescent signal. Furthermore, a fluorescence-colorimetric sensor based on self-assembled MnO 2 -AuNCs-SiO 2 nanocomposites was constructed. Ascorbic acid (AA), the product of sodium l -ascorbyl-2-phosphate (AAP) in the presence of alkaline phosphatase (ALP), can decompose MnO 2 and “turn on” the fluorescence sensor. Moreover, due to the inhibitory effect of OPs on ALP activity, a dual-mode detection method from fluorescence and colorimetry for OPs was developed. The linear range was from 1 μg/L to 50 mg/L with a detection limit of 0.4 μg/L in the fluorescence detection, while the linear range was from 0.1 μg/L to 50 mg/L with a detection limit of 0.09 μg/L in colorimetric analysis. Besides, the in-situ imaging of OPs in fresh vegetables was achieved and a visual semi-quantitative detection of OPs on a test paper was realized. Therefore, the fluorescent biosensor may show a potential value in field detection.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Imran Mahmood Khan,Sobia Niazi,Ye Yu,Ali Mohsin,Bilal Sajid Mushtaq,Muhammad Waheed Iqbal,Abdur Rehman,Wasim Akhtar,Zhouping Wang
Mycotoxins posit serious threats to human and animal health, and numerous efforts have been performed to detect the multiple toxins by a single diagnostic approach. To best of our knowledge, for the first time, we synthesized an aptamer induced “turn on” fluorescence resonance energy transfer (FRET) biosensor using dual-color gold nanoclusters (AuNCs), l-proline, and BSA synthesized AuNCs (Lp-AuNCs and BSA-AuNCs), with WS2 nanosheet for simultaneous recognition of aflatoxinB1 (AFB1) and zearalenone (ZEN) by single excitation. Here, AFB1 aptamer stabilized blue-emitting AuNCs (AFB1-apt-Lp-AuNCs) (at 442 nm) and ZEN aptamer functionalized with red-colored AuNCs (ZEN-apt-BSA-AuNCs) (at 650 nm) were employed as an energy donor and WS2 nanosheet as a fluorescence quencher. With the addition of AFB1 and ZEN, the change in fluorescence intensity (F.I) was recorded at 442 and 650 nm and can be used for simultaneous recognition with a detection limit of 0.34 pg mL–1 (R2 = 0.9931) and 0.53 pg mL–1 (R2 = 0.9934), respectively. Most importantly, the semiquantitative determination of AFB1 and ZEN can also be realized through photovisualization. The current approach paves a new way to develop sensitive, selective, and convenient metal nanocluster-based fluorescent “switch-on” probes with potential applications in multipurpose biosensing.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 10
·107506
·
IF 4.386
Miao Guo,Xia Cui,Lu Wang,Ke Yang,Jiameng Xu,Liangwei Yu,Zhimin Luo,Aiguo Zeng,Jia Zhang,Qiang Fu
Achieving direct electrochemical detection of 17β-estradiol (E2) at low concentrations remains a challenge due to the weak electrochemical activity of E2. In this study, we report a simple, cheap and sensitive electrochemical sensor based on nanocomposite for the direct detection of E2 in wastewater. The nanocomposite sensing electrode was developed by electrochemical in situ polymerization. Due to the π–π stacking and hydrogen bonding between poly-L-tyrosine (P(L-tyr)) with E2, we prepared P(L-tyr) electrode based on dopamine-modified multi-walled carbon nanotubes (PDA-CNTs) and gold nanoclusters (AuNCs). Then P(L-tyr)/AuNCs/PDA-CNTs/GCE sensing system was obtained. Meanwhile, the synergistic effect of fast electron transfer of AuNCs and the signal amplification effect of PDA-CNTs enables sensitive and direct detection of E2. The results showed that P(L-tyr)/AuNCs/PDA-CNTs/GCE sensor had a linear response to E2 concentration from 0.05 to 10 μmol·l−1 with a detection limit of 7.1 nmol·l−1. Then, the trace amount of E2 in pharmaceutical wastewater samples can be directly detected against several interferences from complex matrix, with recoveries from 91.0 to 107.5%. P(L-tyr)/AuNCs/PDA-CNTs/GCE showed good stability when placed at room temperature. Therefore, it can meet the requirements of the sensitive detection of E2 in complex matrix.
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TALANTA
·
2023
·
IF 6.1
Meng Zhang,Jianhong Zhang,Xin Che,Jingjing Jiang,Qin Tu,Jinyi Wang
As one of the essential trace elements in life activities, copper ion (Cu 2+ ) plays a very important role in human health. However, copper-containing pesticides are usually used for sterilization and disinfestation in agriculture. Thus, the residues of copper-containing pesticides in agricultural samples increase the risk of excessive intake of Cu 2+ for human. The development of an effective method for detecting Cu 2+ is still an important task. Herein, a detecting system based on AuNCs@ZIF-8 modified paper and smartphone platform was developed for visual detection of Cu 2+ in agricultural samples. Herein, a detecting system based on AuNCs@ZIF-8 modified paper and smartphone platform was developed for visual detection of Cu 2+ in agricultural samples. In the detecting system, gold nanoclusters (AuNCs) were packaged by ZIF-8 to limit their molecular motion and enhance the fluorescence effectively. In the meanwhile, by ultrasound-assisted biomimetic mineralizing, AuNCs@ZIF-8 composites were uniformly synthesized in situ on the surface of the paper fibers to indicate Cu 2+ by fluorescence quenching . A portable visual monitoring system consisted of the prepared Cu 2+ paper sensor and a smartphone platform was then successfully built and applied to on site detecting Cu 2+ in agricultural samples. The limit of detection (LOD) was 4.57 μM and recovery rate varied from 96.50 % to 121.58 %. The developed detecting system for Cu 2+ has the advantages of easy preparation and operation, and is very suitable for the use in agricultural products and farmland.
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JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Xu Zichen,Xu Hedan,Duan Hongliang,Li Junjie,Hu Xiao,Jiang Kaixin,Wang Guixue,Zhang Yuanyuan
Simple and sensitive detection of cardiac biomarkers is of great significance for early diagnosis and prevention of acute myocardial infarction (AMI). Here, a ratiometric fluorescent nanohybrids probe (AuNCs-QDs) was synthesized through the coupling of bovine serum albumin-functionalized gold nanoclusters (AuNCs) with CdSe/ZnS quantum dots (QDs) to realize simple and sensitive detection of cardiac biomarker myoglobin (Mb). The AuNCs-QDs probe shows pink fluorescence under UV light, with two emission peaks at 468 nm and 630 nm belonging to QDs and AuNCs, respectively. Importantly, the presence of Mb caused fluorescence quenching of the blue-emitting QDs, thereby inhibiting the fluorescence resonance energy transfer (FRET) process between QDs and AuNCs, and reducing the fluorescence intensity ratio (F 468 /F 630 ) of AuNCs-QDs probe effectively. As the concentration of Mb increases, the ratiometric fluorescent probe also exhibits a visible fluorescence color change. The detection limit was as low as 4.99 μg/mL, and the response of the probe to Mb showed a good linear relationship up to 0.52 mg/mL. Moreover, the probe has excellent specificity for Mb. Besides, the AuNCs-QDs has been applied to detect Mb of urine samples. More importantly, we also developed an AuNCs-QDs probe modified smartphone-aided paper-based strip for on-site monitoring of Mb. As far as we know, this is the first report of a smartphone-aided paper-based strip for on-site quick monitoring of Mb, which provides a useful approach for AMI biomarker monitoring and may can be extended to other medical diagnostics.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Luting Yan,Yuhao Zhang,Qi Guo,Lei Tian,Falan Li,Qingqing Yang,Yanyan Zhang
Most electrochemiluminescent (ECL) biosensors currently necessitate coreactants that affect sensor detection accuracy and increase sensor construction complexity. This study detailed the creation of an ECL biosensor without coreactant, utilizing Methionine-capped Au nanoclusters (Met-AuNCs) as luminophore and a Pb 2+ -dependent DNAzyme-mediated DNA walker signal amplification strategy to detect Vibrio parahaemolyticus (Vp) in scallops. Double-stranded DNA (dsDNA) was produced through hybridization of the Vp aptamer with DNA walker. The electrode surface was loaded with cDNA labeled with Met-AuNCs. Upon the presence of Vp, it competitively bound to the aptamer, resulting in dsDNA dissociation and exposing the DNAzyme sequence in the DNA walker. The introduction of Pb 2+ initiated the activation and circulation of the DNAzyme-mediated DNA walker on the electrode surface, causing the cDNA to be sheared. As a result, the cDNA-Met-AuNCs on the surface of the electrode decreased, resulting in a reduction of ECL signal intensity. The ECL biosensor detected Vp at a range of 1.00×10 1 -1.00×10 7 CFU/mL, under optimal conditions, with the limit of detection (LOD) being 1.23 CFU/mL. In this work, the biosensor demonstrated excellent stability and specificity and had proven successful in detecting actual samples of aquatic products. This study offers a novel ECL method without a coreactant for the detection of foodborne pathogens in aquatic products.
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MOLECULES
·
2024
·Vol. 29
·期 24
·6011
·
IF 4.2
Ting Zhou,Luyao Zang,Xia Zhang,Xia Liu,Zijie Qu,Guodong Zhang,Xiufeng Wang,Fang Wang,Zhiqing Zhang
Gold nanoclusters (AuNCs) have been widely investigated because of their unique photoluminescence properties. However, the applications of AuNCs are limited by their poor stability and relatively low fluorescence. In the present work, we developed nanocomposites (L-Cys-AuNCs@ZIF-8) with high fluorescence and stability, which were constructed by encapsulating the water-dispersible L-Cys-AuNCs into a ZIF-8 via Zn2+-triggered growth strategy without high temperature and pressure. The maximum emission wavelength of the L-Cys-AuNCs@ZIF-8 composite was at 868 nm, and the fluorescence intensity of L-Cys-AuNCs@ZIF-8 was nearly nine-fold compared with L-Cys-AuNCs without the ZIF-8 package. The mechanism investigation by fluorescence spectroscopy and X-ray photoelectron spectroscopy showed that L-Cys-AuNCs@ZIF-8 impeded ligand rotation, induced energy dissipation, and diminished the self-quenching effect, attributing to the spatial distribution of L-Cys-AuNCs. Based on the high fluorescence efficiency of L-Cys-AuNCs@ZIF-8, a “signal off” detective platform was proposed with copper ions as a model analyte, achieving a sensitive detection limit of Cu2+at 16.7 nM. The quenching mechanism was confirmed, showing that the structure of the L-Cys-AuNCs@ZIF-8 nanocomposites was collapsed by the addition of Cu2+. Attributing to the strong adsorption ability between copper ions and pyridyl nitrogen, the as-prepared L-Cys-AuNCs@ZIF-8 was shown to accumulate Cu2+, and the Zn2+in ZIF-8 was replaced by Cu2+.
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