RSC Advances
·
2020
·Vol. 10
·期 28
·16277-16283
·
IF 3.119
Bobo Huang,Bin Zhang,Bo Liang,Lu Fang,Xuesong Ye
Hepatocellular carcinoma (HCC) is one of the most common cancerous diseases, with a low 5 year survival rate. Global hypomethylation drives genomic instability, which is regarded as one biomarker for early diagnosis. Long interspersed nucleotide element-1 (LINE-1) makes up around 17% of the genome, and could be regarded as a surrogate marker for global DNA methylation. In this work, a gold nanoparticle (AuNP) modified carbon fiber microelectrode (CFME) with a diameter of 7 μm was applied for the first time to detect the methylation level of LINE-1, by distinguishing adsorption affinities between different DNA bases and AuNPs. Several parameters, including AuNP electrodeposition time, sample adsorption time, and DNA concentration have been analyzed and optimized. The detection limit of our assay was 0.1 nM with only 2 μL sample solution. And the CFME had an excellent sensitivity of 10% methylation change and had the capacity to distinguish only one methylated CpG site. The global DNA methylation level of real samples including cell lines and clinical tissues was tested. Higher signals of HCC cell lines and cancer tissues were observed respectively, compared with normal hepatic cell lines and normal tissues. This work provides a promising approach for HCC early diagnosis and prognosis.
研究领域:
癌症生物学
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分子生物学
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生物医学工程
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yue He,Jing Zhou,Ruijie Fu,Yanlin Liu,Yiwen Wang,Haoran Liu,Jing Zhao,Yongliang Cui,Bining Jiao
The monitoring of the fungal genus Alternaria , which causes destructive brown spot disease in citruses worldwide and produces highly toxic mycotoxins , is extremely important to protect citrus and human health. In this work, we describe an ultrasensitive colorimetric method for the detection of genomic DNA of Alternaria from citrus fruit samples, using a system consisting of five groups of reporter probes. Each reporter probe is prepared by coupling recognition DNA and horseradish peroxidase (HRP) on the surface of gold nanoparticle (AuNP) through a convenient and low-cost freezing-assisted method. Meanwhile, the capture DNA is immobilized on magnetic bead (MB) via biotin-streptavidin reaction. Then, the capture DNA, target DNA, and five groups of AuNP-based reporter probes form a stable DNA-heptamer sandwich structure on the MB, and then HRP generates a blue signal for the subsequent colorimetric detection. It should be noted that AuNP with a large specific surface area drives abundant HRP anchoring, resulting in significant signal amplification. In addition, there are five groups of AuNP-based reporter probes, which further amplify the detection signal. As a result, the detection limit of the artificial target DNA is as low as 15.6 pM. Because the detection signal can be recorded visually without any special equipment, and its sensitivity is high, this method represents a suitable diagnostic tool for Alternaria genetic detection.
研究领域:
植物病理学
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分子诊断
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食品安全
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yonghong Zha,Yansong Li,Pan Hu,Shiying Lu,Honglin Ren,Zengshan Liu,Hualin Yang,Yu Zhou
Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0–106.6% from a corn sample, which are in good agreement with the commercial kit (R2 = 0.9995). This “turn-off” fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.
研究领域:
分析化学
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环境科学
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公共卫生
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纳米技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·
IF 2.6
Ren Jing,Tang Jing,Zhao Mei,Chen Zilin,Wang Fang
Uracil (U) in DNA plays an important role in numerous biological processes. It can be introduced through two primary mechanisms, cytosine deamidation and misincorporation of dUMP during DNA replication. The misincorporation of U in DNA is associated with various diseases, especially cancer. Here, we developed an electrochemical method utilizing a modified electrode with self-assembled gold nanoparticles on covalent organic frameworks (COF-V-AuNPs) for the direct detection of U. The COF-V-AuNP composite offers numerous advantages, such as exceptional stability, abundant π-functional sites, excellent conductivity, a high specific surface area, a porous structure, and a strong non-covalent affinity, all of which facilitate sensitive detection of U. This electrochemical method achieves a detection limit of 1.92 μM for U. Furthermore, the method was successfully applied to the detection of U using human serum samples as matrices, indicating its potential for clinical applications.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 10
·2147
·
IF 6.525
Zhijun Guo,Dan Sun,Xian Zhou,Huan Xu,Yizhou Huang,Chenglin Chu,Baolong Shen
In this work, a facile direct current atmospheric pressure micro-plasma (APM) technology was deployed for the synthesis of functional gold nanoparticle/chitosan (AuNP/CS) nanocomposites for the first time. Different experimental parameters, such as metal salt precursor concentration and chitosan viscosity, have been investigated to understand their effects on the resulting nanocomposite structures and properties. The nanocomposites were fully characterized using a wide range of material characterization techniques such as UV–vis, transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectra and X-ray photoelectron spectroscopy (XPS) analyses. Potential reaction pathways have been proposed for the nanocomposite synthesis process. Finally, potential of the synthesized nanocomposites towards photothermal conversion and bacteria eradiation applications has been demonstrated. The results show that APM is a facile, rapid and versatile technique for the synthesis of AuNP/CS functional nanocomposites. Through this work, a more in-depth understanding of the multi-phase system (consisting of gas, plasma, liquid and solid) has been established and such understanding could shine a light on the future design and fabrication of new functional nanocomposites deploying the APM technique.Keywords:atmospheric pressure micro-plasma;plasma induced liquid chemistry;gold nanoparticles;chitosan;nanocomposites;photothermal conversion;antibacterial
研究领域:
材料科学
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生物医学工程
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纳米技术
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DOI
PubMed
高影响因子
Materials Advances
·
2022
·Vol. 3
·期 23
·8677-8683
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IF 0
Jiameng Wang,Wuyi Zhang,Lanlan Chen,Jie Huang,Xiaojia Shi,Dong Han,Jia Wen,Hongyuan Yan
A kind of nanocomposite consisting of molybdenum disulfide (MoS2) and gold nanoparticles (AuNPs) was prepared by a simple self-reduction method. Transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy were used to characterize the MoS2@AuNP nanocomposites. The as-prepared MoS2@AuNP nanocomposites were further used to modify a glassy carbon electrode to construct an electrochemical glucose biosensor. The electrochemical performance of the biosensor was evaluated using cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry. And it was found that the biosensor had prominent electrochemistry activity towards glucose. Under the optimized experimental conditions, a linear relationship in the range of 1–100 μM was detected between the difference of cathodic reduction current and the concentration of glucose, and the detection limit was 0.14 μM. The results showed that the biosensor exhibited good specificity, repeatability and reproducibility. In addition, the biosensor was also successfully applied for the determination of glucose concentrations in milk samples.
研究领域:
材料科学
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生物医学工程
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分析化学
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DOI
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 19
·56198-56206
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IF 5.8
Wang Tianshu,Shi Peibo,Wang Mingyu,Zhang Shaojun
Oily sewage discharged from ships has brought many harms to the marine environment, even endangered marine life and human life. As a new type of water treatment technology, microbial fuel cell (MFC) can efficiently treat pollutants and recover energy, which can be converted into electric energy. However, its large internal resistance restricts its development. In order to solve the problems of low power generation performance and poor biocompatibility of microbial fuel cell, a gold nanoparticle-carbon quantum dot/polydopamine/graphene oxide/bacterial cellulose (AuNP-CQD/PDA/GO/BC) electrode was prepared, and it was applied to the treatment of oily sewage from ships. Fourier transforms infrared spectroscopy, X-ray diffraction, scanning electron microscopy, gas chromatography-mass spectrometry, and contact angle measuring instrument were used to characterize the electrode. The results show that PDA bridges GO and AuNP-CQD particles through the electrostatic interaction/π-π bond/hydrogen bonding, respectively. This attracts a large number of microorganisms to attach to the surface of the porous anode material, which greatly improves the activity and quantity of microorganisms. Moreover, the maximum power density of AuNP-CQD/PDA/GO/BC electrode is 2624.91 mW/m 2 , which obviously improves the electrochemical performance of MFC. The oil content of the treated water is ≤ 15 mg/L, reaching the discharge of MARPOL 73/78 convention. Therefore, the proposed approach has paved new dimensions in not only the preparation of a new composite electrode materials but also its applications as effective degradation of ship oily sewage in MFC.
研究领域:
环境科学
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材料科学
·
微生物学
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DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Xiaoting Liu,Ling Lu,Nan Zhang,Wei Jiang
Apoptosis is a typical programmed death mode with complex molecular regulation mechanisms. Developing advanced strategies to monitor apoptosis progression is conducive to disease treatment related with apoptosis. Herein, we developed a regulator-carrying dual-responsive integrated AuNP composite fluorescence probe for in situ real time monitoring apoptosis progression. The nanoprobe is constructed by modifying specially designed double-stranded DNA (dsDNA) and caspase 3-specific cleavable peptides (pep) to the surface of AuNP. After uptake by cells, the nanoprobe recognizes miRNA 21 and triggers fluorescence recovery, enabling silencing and imaging of the upstream signaling molecule miRNA 21. Once miRNA 21 is silenced, the downstream signaling molecule caspase 3 is activated and cleaves the substrate peptides, and fluorescence is restored for in situ imaging of caspase 3. The apoptosis induced by silencing miRNA 21 has been successfully implemented in HeLa and A549 cells. The expression level of miRNA 21 and corresponding changes of caspase 3 have also been effectively monitored. These results suggested this nanoprobe will be a potential tool for apoptosis-related biomedical research and clinical application.
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PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 27
·9447-9456
·
IF 7.367
Yao Yu,Youshen Wu,Jiajun Liu,Yiming Liu,Daocheng Wu
On the basis of finite-difference time-domain simulations, a novel strategy is developed to prepare highly efficient bis(2-(2′-benzothienyl)pyridinato-N, C3′)iridium (BTP) and AuNP polyiohexol composite nanoparticles (BAPI NPs) as dual-modal phosphorescence/computed tomography (CT) bioprobes. In these bioprobes, BTP and AuNPs are both encapsulated with polyiohexol NPs. All bioprobe components perform two functions here: AuNPs could be used as both a CT contrast agent and a phosphorescence enhancement reagent of BTP with a metal-enhancement fluorescence effect. The results showed that BAPI NPs were almost spherical in shape, with an average size of 50.36 ± 3.8 nm and a higher total contrast agent loading ratio of 69.4%. Fourier transform infrared spectra confirmed that AuNPs and BTP are encapsulated in BAPI NPs. It is shown that they have lower toxicity for tissues and cells, their phosphorescence intensities are 8.27-fold that of BTP polyiohexol NPs (BPI NPs), the average phosphorescence intensity of the BAPI NPs was 1.46 times higher than that of BPI NPs and 5.85 times that of BTP alone in vivo. Improved CT imaging is obtained at a low dose of polyiohexol in vivo. These bioprobes not only have highly efficient and excellent dual-modal imaging, but they also save the use of various materials, indicating that these bioprobes are the potential dual-modal probes of the future.
研究领域:
生物医学工程
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纳米医学
·
分子影像学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiao-Yuan Wang,Qin Hong,Ze-Rui Zhou,Zi-Yue Jin,Da-Wei Li,Ruo-Can Qian
The localized surface-plasmon resonance of the AuNP in aqueous media is extremely sensitive to environmental changes. By measuring the signal of plasmon scattering light, the dark-field microscopic (DFM) imaging technique has been used to monitor the aggregation of AuNPs, which has attracted great attention because of its simplicity, low cost, high sensitivity, and universal applicability. However, it is still challenging to interpret DFM images of AuNP aggregation due to the heterogeneous characteristics of the isolated and discontinuous color distribution. Herein, we introduce machine vision algorithms for the training of DFM images of AuNPs in different saline aqueous media. A visual deep learning framework based on AlexNet is constructed for studying the aggregation patterns of AuNPs in aqueous suspensions, which allows for rapid and accurate identification of the aggregation extent of AuNPs, with a prediction accuracy higher than 0.96. With the aid of machine learning analysis, we further demonstrate the prediction ability of various aggregation phenomena induced by both cation species and the concentration of the external saline solution. Our results suggest the great potential of machine vision frameworks in the accurate recognition of subtle pattern changes in DFM images, which can help researchers build predictive analytics based on DFM imaging data.
研究领域:
纳米生物学
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医学影像学
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人工智能
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xingyue Tang,Yafang Shen,Jiangqi Wang,Zhaohui Qiao,Fangkai Han,Yanna Cui,Qiuyue Hu,Yuxin Wu,Jiahua Wang,Xiaodan Liu,Huang Dai,Guijie Hao
Sensitive and accurate detection of enrofloxacin (ENR) residues is essential for food safety. Herein, a competitive immunosensor was developed for the detection of ENR, using a gold nanoparticle (AuNP)-enhanced CRISPR/Cas12a system. In this method, bovine serum albumin-ENR- and single-stranded DNA-modified AuNPs (AuNPs-BSA-ENR/ssDNA) was used to act as both a competitor and CRISPR/Cas12a activator. The use of AuNPs-BSA-ENR/ssDNA helped transduce the target recognition information to the cleavage activity of the CRISPR/Cas12a system. Moreover, benefiting from the high ssDNA loading capability of AuNPs, it enabled one-to-multiple CRISPR/Cas12a activation. The synergetic signal amplification from AuNPs and the CRISPR/Cas12a system guaranteed the sensitivity of this method with a limit of detection of 1 ng/mL. Its feasibility for the detection of food and environmental samples was also investigated with recovery rates ranging from 91.0% to 115.2%. The developed immunosensor offered a promising alternative for ENR detection.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
分子诊断
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 44
·7765-7770
·
IF 0
WeiJuan Yang,Ye He,LiangJun Xu,Danlong Chen,Mengxue Li,Hongyan Zhang,FengFu Fu
In this paper, we have reported on novel phosphatidylserine-functionalized AuNPs (gold nanoparticles) for the visual detection of Cu2+ by employing phosphatidylserine for Cu2+ recognition and AuNPs for signal generation. The phosphatidylserine (1,2-dioleoyl-sn-glycero-3-phospho-L-serine, DOPS) was covalently assembled on the AuNP surface to obtain DOPS-functionalized AuNPs. It was demonstrated that DOPS-functionalized AuNPs could specifically bind Cu2+ and lead to the aggregation of AuNPs, which gave rise to a colour change from wine-red to blue and a new absorption band around 650 nm. This provides a sensing platform for the simple, rapid and field portable colorimetric detection of Cu2+. By using the sensing platform, a selective and sensitive visual biosensor for the detection of Cu2+ was developed. The proposed biosensor has outstanding analytical advantages such as good stability, relatively high sensitivity, low cost and short analysis time. It can be used to detect concentrations of Cu2+ as low as 30 μM in river water by observation with the naked eye and of 1.55 μM Cu2+ in river water by UV-visible spectrophotometry, within 10 min and with a recovery of 98–103% and a relative standard deviation (RSD) < 4% (n = 6). The proposed biosensor is promising for on-site detection of trace Cu2+ in clinical diagnosis or environmental monitoring.
研究领域:
分析化学
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环境科学
·
生物医学工程
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产品关联
DOI
PubMed
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