SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Shuyan Xiao,Jiping Lu,Liang Sun,Shengli An
A new colorimetric biosensor for specific detection of azlocillin was developed by using DNA aptamer as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric indicator. In the absence of azlocillin, the AuNPs were protected by the aptamer and stabilized at high NaCl concentrations, displaying a red solution. In the presence of azlocillin, the aptamer reacts specifically with azlocillin, resulting in the aggregation of AuNPs and an apparent red to blue color change. The characteristic change can be easily observed by the naked eye and quantitatively detected by an ultraviolet–visible (UV–Vis) spectrometer. Under the optimal conditions, the absorbance variation at 522 nm (ΔA 522 ) of AuNPs changed proportionally with increasing concentration of azlocillin, which exhibited a linear relationship in the concentration range of 50 nM to 500 nM, with a detection limit of 11.6 nM. Furthermore, the aptasensor was successfully used to detect azlocillin in milk and tap water samples, with recoveries ranging from 97.64% to 102.21% and a relative standard deviation (RSD) less than 3.81%.
研究领域:
分析化学
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生物传感器
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纳米技术
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环境科学
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DOI
PubMed
高影响因子
Applied Sciences-Basel
·
2024
·Vol. 14
·期 5
·2118
·
IF 2.5
Mengping Huang,Shuai Ma,Jinrong He,Wei Xue,Xueyan Hou,Yuqi Zhang,Xiaofeng Liu,Heping Bai,Ran Li
Amino acids found in minor coarse cereals are essential for human growth and development and play a crucial role in efficient and rapid quantitative detection. Surface-enhanced Raman spectroscopy (SERS) enables nondestructive, efficient, and rapid sample detection. Traditional SERS detection efficiency is constrained by the use of a single target. In this study, three different amino acids (cysteine, valine, and tryptophan) were detected simultaneously using a ZIF-8@AuNPs composite substrate. The linear range of detection was 10−3to 10−1M, with limits of detection (LODs) of 2.40 × 10−4M, 2.24 × 10−4M, and 1.55 × 10−4M, respectively. Same linear ranges and LODs were achieved with a one-dimensional convolutional neural network method. Furthermore, this substrate enabled the effective detection of amino acids in millet and efficient detection of cysteine in health products. This study presents a novel method for simultaneous detection of multiple analytes.
研究领域:
分析化学
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生物医学工程
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食品科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zifei Wu,Hao Ding,Kailai Xu,Chenghui Li,Xiandeng Hou
Background due to the toxicity and widespread distribution of inorganic mercury ions (Hg 2+ ), it is a crucial task for researchers to develop simple methods for field analysis of water samples for trace Hg 2+ . Result a colorimetric and ratiometric fluorescent dual-mode assay based on bifunctional UiO–(COOH) 2 –NH 2 /Eu and gold nanocomplexes (AuNPs@UiO–(COOH) 2 –NH 2 /Eu) was constructed for the sensitive, selective and potentially on-site determination of Hg 2+ . The incorporation of Hg 2+ resulted in the formation of gold amalgam, which enhanced peroxidase-like activity of AuNPs@UiO–(COOH) 2 –NH 2 /Eu, accelerating the oxidization of zymolyte 3,3′,5,5′-tetramethylbenzidine (TMB). Concurrently, the color of the reaction solution turned blue, and the RGB (red, green, and blue) of the solution color changed accordingly. The fluorescence at 618 nm was almost unchanged with the increase of Hg 2+ concentration, whereas the fluorescence at 460 nm was obviously quenched due to the inner filter effect (IFE). Under UV irradiation, the fluorescence emission of the sensing system changed from blue to red, enabling easy visual detection. The probe showed great linearity over a wide range of 20–1200 nM (colorimetric) and 50–4000 nM (fluorescent), with limits of detection (LODs) of 8 nM and 3 nM, respectively. Significance the proposed method was successfully applied to the determination of trace Hg 2+ in tap water and groundwater, while the use of a smartphone enables its simplicity and portability. The results show that the colorimetric and ratiometric fluorescent dual-mode method based on AuNPs@UiO–(COOH) 2 –NH 2 /Eu can achieve rapid visual determination of Hg 2+ potentially useful for on-site analysis.
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
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2019
·Vol. 9
·期 1
·1901229
·
IF 6.27
Shi-Zhao Lu,Xiao-Yan Guo,Mei-Shuai Zou,Zi-Qin Zheng,Yu-Chuan Li,Xiao-Dong Li,Li-Li Li,Hao Wang
The emergence of drug-resistant bacteria is becoming the focus of global public health. Early-stage pathogen bioimaging will offer a unique perspective to obtain infection information in patients. A photoacoustic (PA) contrast agent based on functional peptide modified gold nanoparticles (AuNPs@P1) is developed. These nanoparticles can be specifically tailored surface peptides by bacterial overexpressed enzyme inducing in situ aggregation of the gold nanoparticles. In the meantime, the close aggregation based on the hydrogen bonding, π–π stacking, and hydrophobic interaction of the peptide residues on the surface of gold nanoparticles exhibits a typical redshifted and broadened plasmon band. In addition, this active targeting and following in situ stimuli-induced aggregation contribute to increased nanoparticle accumulation in the infected site. Finally, the dynamic aggregation of AuNPs@P1 results in dramatically enhanced photoacoustic signals for bioimaging bacterial infection in vivo with high sensitivity and specificity. It is envisioned that this PA contrast agent may provide a new approach for early detection of bacterial infection in vivo.
研究领域:
生物医学工程
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纳米技术
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医学成像
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微生物学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
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2017
·Vol. 33
·期 1
·119-124
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IF 1.509
Nan Liu,Juan Hao,Keying Cai,Mulan Zeng,Zhenzhong Huang,Lili Chen,Bingxian Peng,Ping Li,Li Wang,Yonghai Song
A novel ratiometric fluorescence nanosensor for superoxide anion (O 2 •− ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb 3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O 2 •− . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O 2 •− demonstrated high sensitivity and selectivity with a wide linear response range (14 nM–10 μM) and a low detection limit (4.7 nM).
研究领域:
分析化学
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Nanfei Yang,Yuncheng Bei,Yahong Huang,Wei Zheng,Jiehua Ma,Jiangqiong Ke
Serum Cystatin C (CysC) is an impressive marker for early diagnosis of renal dysfunction. In this work, we established a novel electrochemical immunosensor based on Fe 3 O 4 /AuNPs-MWCNTs@PDA nanocomposite for the detection of CysC. The Fe 3 O 4 /AuNPs-MWCNTs@PDA nanozyme complex by polydopamine encapsulation can not only carry massive detection antibodies, but also bind the electroactive substance toluidine blue (TB) through electrostatic adsorption. By immobilizing AuNPs onto the electrode to bind the capture antibody (Ab1), we constructed a sandwich electrochemical immunosensor with low cost, high sensitivity, and repeatability. The detection range is 3.9–125.0 ng/mL with a significant linear relationship between the current peak difference (ip) and logarithm of the CysC concentration. Moreover, the detection limit of the immunosensor is 0.157 ng/mL. We have successfully utilized this novel immunosensor to detect CysC in human serum samples, and these results have implications for its potential use in clinical application.
研究领域:
生物医学工程
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分析化学
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临床诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Ruhao Wang,Mingjing Wang,Xu Hun
Coxsackievirus B3 (CVB3), a member of the genus Enterovirus, has high risks for incidence and mortality. Developing speedy and sensitive molecular diagnostics for CVB3 detection is challenging. Herein, a novel highly sensitive photoelectrochemical (PEC) biosensor based on nanocomposite (ZnSeNSs/AuNPs/BNNSs) modified electrode was fabricated. This biosensor relied on reverse transcription of CVB3 RNA and the rapid isothermal amplication by recombinase polymerase amplification (RPA). The amplified product was identified and linked to photoelectrode. Then biotin on the amplified product was connected to strepavidin-alkaline phosphatase (SA-ALP). The alkaline phosphatase catalyzed the hydrolysis of substrate 4-aminophenylphosphate monosodium (4-APP) to generate free 4-aminophenol which was a electron donor. An enhancing PEC signal was obtained. Therefore, a relationship between photocurrent response and CVB3 concentration was established. Under the optimal conditions, this PEC biosensor displayed a liner photocurrent response in the range of 0.1 fM ∼100 fM with a low detection limit of 30 aM. Furthermore, this strategy with satisfactory stability and selectivity provides an innovative way for CVB3 detection and lays a foundation for the research of portable devices for clinical sample processing and analysis in the further.
研究领域:
病毒学
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生物化学
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生物医学工程
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分析化学
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yunliang Liu,Jing Gao,Yuting Huo,Yang Yang,Daoping Yue,Xianghang Lin
MiRNA-155 is a reliable biomarker for cancers and allergic rhinitis, also plays a critical role in regulating gene expression and mediating the allergic TH2 process. The accurate determination of miRNA-155 will be significant for diagnosing allergic rhinitis, especially for children. Herein, Yb-Bi2S3 nanorods were prepared through a hydrothermal method. Gold nanoparticles (AuNPs) were assembled onto Yb-Bi2S3 nanorods surface to provide a Yb-Bi2S3/AuNPs nanocomposite. Yb-Bi2S3/AuNPs nanocomposite was used as a photoactive element for fabricating a near-infrared photoelectrochemical aptasensing platform by assembling the aptamer for specific recognition of miRNA-155. The photocurrent variation of the photoelectrochemical aptasensor before and after recognizing miRNA-155 is linearly related to the miRNA-155 concentration in the range of 1.0 fmo l L-1 ~ 1.0 nmo l L-1. The detection limit was calculated to be 0.34 fmol L-1. The developed photoelectrochemical aptasensor was successfully employed for determining miRNA-155 in serum samples with satisfactory results, indicating possessing practical applications in the determination of miRNA-155 in real samples.
研究领域:
生物医学工程
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分子诊断学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Biao Jia,Baoshan He,Renli Liu,Jinping Yang,Wenjie Ren,Zhiguang Suo,Yiwei Xu
The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study,the conductivity of aptasensor was improved by modifying carbonized MOF as the base material. In the presence of fipronil (FIP), Apt tended to bind to FIP and was separated from the electrode surface, so that the remaining single-stranded complementary DNA (cDNA) were hybridized with signal probe (MB@PtPdCo/sDNA) and amplified the electrochemical signal. By modifying the substrate, competitive amplification strategy and signal probe material to catalyze signal molecules, the sensor performs multiple signal amplification, and the electrical signal is significantly improved. The sensor has a wide detection range 100 pg/mL to 100 μg/mL and a low detection limit of 76 pg/mL.
研究领域:
分析化学
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环境科学
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食品安全检测
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2019
·
IF 4.248
Yuanyuan Hui,Bini Wang,Rong Ren,Aiqing Zhao,Fuxin Zhang,Shuanghong Song,Yaping He
It is very essential and meaningful for detection and quantitation of aflatoxin in food and agricultural commodities to establish a rapid and accurate detection method. Herein, a novel electrochemical aptasensor for sensitive and selective detection of aflatoxin B 1 (AFB 1 ) was successfully constructed. The thiolated complementary strand (cDNA) of the AFB 1 aptamer (Apt) was immobilized on the surface of the glassy carbon electrode (GCE) modified with gold nanoparticles (AuNPs) through Au–S bond. Then Apt was attached on the surface of GCE via by specific base pairing. In the presence of AFB 1 , the Apt was detached from the electrode surface by specific recognition between the AFB 1 and Apt, forming Apt-AFB 1 conjugates in solution. The conjugates were digested by exonuclease I ( Exo I) to trigger AFB 1 recycling. Finally, DNA-AuNPs-horseradish peroxidase (DNA-AuNPs-HRP) nanoprobes was bound with cDNA on the surface of electrode by specific base pairing. HRP could catalyze the oxidation of hydroquinone (HQ) to benzoquinone (BQ) by hydrogen peroxide (H 2 O 2 ) for producing a strong electrochemical signal. Under the optimized conditions, the electrochemical signals increased with increasing concentrations of AFB 1 in a range from 10 −3 ng mL −1 to 200 ng mL −1 with a low detection limit of 3.3 × 10 −4 ng mL −1 . Moreover, the aptasensor has been successfully applied for the determination of AFB 1 in peanuts and corn samples and the recoveries were 88.5 %–110.2%, which would provide a novel method for the rapid detection of AFB 1 in food samples.
研究领域:
食品安全检测
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生物传感技术
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Xujie Su,Yujie Han,Zhiguang Liu,Lifang Fan,Yujing Guo
Simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. This paper describes an alternative method for detection of nucleolin, which is a major nucleolar protein, overexpressed in several types of cancer, and is a cancer-specific target. Gold nanoparticles/molybdenum disulfide/reduced graphene oxide (AuNPs/MoS 2 /rGO) was easily synthesized with hydrothermal method and characterized by transmission electron microscopy (TEM), x-ray diffraction (XRD) and x-ray photoelectron spectroscopy (XPS). Combining the properties of large surface area and good electrical conductivity of AuNPs/MoS 2 /rGO with the high affinity and specificity of aptamer, a smart hierarchically self-assembled electrochemical aptasensor was established for the specific and sensitive nucleolin (as a model analyte) detection. Under the optimized conditions, AuNPs/MoS 2 /rGO based aptasensor exhibits a great performance for the detection of nucleolin with a linear response from 0.5 nM to 1.0 μM and a low detection limit of 0.16 nM. In addition, this electrochemical aptasensor has good reproducibility towards nucleolin. Besides, the practicability of the TNA (thiolated nucleolin aptamer)/AuNPs/MoS 2 /rGO was demonstrated by analysis of nucleolin in the blood sample. This work paves a promising way for the facile synthesis and design MoS 2 /rGO nanocomposite-based electrochemical aptasensor for testing tumor markers in clinical diagnostics.
研究领域:
分析化学
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生物医学工程
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癌症诊断
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-13
·
IF 5.3
Guo Liang,Zhou Shijin,Xue Jinyan,Liu Zenghui,Xu Shuqing,He Zhangxu,Yang Huaixia
A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core–shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring. Graphical Abstract
研究领域:
分析化学
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食品安全
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环境监测
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）