BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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2021
·Vol. 85
·期 8
·1809-1817
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IF 2.043
Li Qiang,Zhai Chunmiao,Wang Guodong,Zhou Jia,Li Weiguang,Xie Liquan,Shi Zhanli
Ginsenoside Rh1 (Rh1) has anti-inflammatory effects in asthma mice, but the underlying mechanism remains unclear. BALB/c mice were sensitized and challenged with ovalbumin (OVA) to construct asthma model. Mice received Rh1 or tiotropium bromide 0.5 h before OVA challenge. Airway morphology and airway remodeling were assessed by HE staining and Masson's trichrome staining, respectively. Th1/Th2 cytokines in serum or broncho alveolar lavage fluid (BALF) were measured by ELISA kits. Rh1 significantly alleviated the lung resistance and airway resistance, and reduced the number of total inflammation cells, eosinophils, neutrophils, and lymphocytes in BALF of the asthmatic mice. The morphological changes and collagen deposition of airway were also reduced by Rh1 in asthmatic mice. The increase of Eotaxin, IL-4, IL-5, IL-13, and IL-33 and the decrease of IL-12 and IFN-γ in both BALF and serum of OVA exposed mice were reversed by Rh1. Rh1 attenuates OVA-induced asthma in the mice model by regulating Th1/Th2 cytokines balance.
研究领域:
免疫学
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药理学
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呼吸病学
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DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2021
·Vol. 15
·期 4
·1110-1125
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IF 4.102
Tian He,Wenxiang Zhu,Mingyou Liu,Xiaofei Tian,Fangeng Chen
Highly reactive polyols were prepared from modified acetic acid lignin (AAL) by a two-step combination of mild hydrothermal degradation and hydroxylation. The AAL-based polyols became relatively reactive and compatible with diisocyanate, with a reduction in the number average molecular weight (M n ) from 2923 to 684 g/mol and an increase in the hydroxyl content from 5.21 to 9.13 mmol/g. The mechanical performance and thermal properties of the AAL-based polyurethane elastomers (LPUEs) strongly depended on the molecular weight and hydroxyl content of the AAL-based polyols in the partial substitution of polytetramethylethylene ether glycol (PTMEG). This study proposed the prospective utilization of AAL as an alternative resource in the synthesis of polymers and biomaterials. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2021
·Vol. 85
·期 7
·1697-1705
·
IF 2.043
Chen Jianmin,Ran Mengnan,Wang Meixia,Liu Xinying,Liu Siwan,Yu Yue
The related structure–activity relationships of cinnamic acid and its derivates have not been studied in details yet. Herein, antityrosinase and antioxidant activities of 18 compounds were evaluated. The results demonstrated that the substituents on the phenyl ring of cinnamic acid led to the enhancement of the inhibition on monophenolase and the weakening of the inhibition on diphenolase. Among these tested compounds, 9 was first discovered as a tyrosinase inhibitor in a reversible competitive manner with IC50 value of 68.6 ± 4.2 µm. Docking results demonstrated 9 located into the catalytic center of tyrosinase. Antioxidant assay indicated that only 1 hydroxyl group on the phenyl ring was not enough to possess the radical scavenging activity, and the number of hydroxyl groups may be more important. This study will be helpful in the development of new cinnamic acid derivates as tyrosinase inhibitors and antioxidants with higher efficacy.
研究领域:
药理学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2021
·Vol. 85
·期 7
·1665-1674
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IF 2.043
Feng Wei
This study aims to explore the effect of Tectorigenin in chronic cerebral ischemia (CCI)-induced cognitive impairment mice model. Cognitive impairment, hippocampal tissue histopathology, and myelin density in CCI mice were detected. HT22 cells were used to induce oxygen–glucose deprivation/reperfusion (OGD/R) injury. Cell viability and apoptosis of transfected HT22 cells and toll-like receptor-4 (TLR4)/nuclear factor-kappaB (NF-κB) pathway–related factor levels in hippocampal tissue and OGD/R models were detected. CCI caused cognitive impairment, hippocampal damage, and decreased myelin density in mice while promoting interleukin-1β, tumor necrosis factor-alpha, TLR4, myeloid differentiation primary response gene 88, p-p65, NLRP3, and ASC levels. Tectorigenin reversed the effects of CCI in mice and reversed the promoting effects of OGD/R on apoptosis and TLR4/NF-κB pathway–related factors levels, while overexpressed TLR4 reversed the effects of Tectorigenin in OGD/R-induced HT-22 cells. Tectorigenin alleviated cognitive impairment in CCI mice by inhibiting the TLR4/NF-κB signaling pathway.
研究领域:
神经科学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2020
·Vol. 85
·期 4
·786-797
·
IF 1.516
Wang Yuanyuan,Guo Shanqi,Jia Yingjie,Yu Xiaoyu,Mou Ruiyu,Li Xiaojiang
Prostate cancer (PCa) is one of the important factors of cancer deaths especially in the western countries. Hispidulin (4′,5,7-trihydroxy-6-methoxyflavone) is a phenolic flavonoid compound proved to possess anticancer properties, but its effects on PCa are left to be released. The aims of this study were to investigate the effects and the relative mechanisms of Hispidulin on PCa development. Hispidulin administration inhibited proliferation, invasion, and migration, while accelerated apoptosis in Du145 and VCaP cells, which was accompanied by PPARγ activation and autophagy enhancement. The beneficial effects of Hispidulin could be diminished by PPARγ inhibition. Besides, Hispidulin administration suppressed PCa tumorigenicity in Xenograft models, indicating the anticancer properties in vivo. Therefore, our work revealed that the anticancer properties of Hispidulin might be conferred by its activation on PPARγ and autophagy.
研究领域:
肿瘤学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2024
·
IF 3.2
Yue Ouyang,Ying Liu,Longjin Tian,Yongyu Ding,Guozhi Fan,Cheng Pan,Qunpeng Cheng
Anaerobic digestion is a promising technology for the treatment of food waste (FW) but it requires optimization to improve its performance. This study investigated whether combining the addition of biochar with lipase pretreatment could improve the anaerobic digestion of FW. The results showed that biochar stimulated the release of soluble substances, resulting in an increase in the methane yield of groups with added biochar by 16.24% to 19.36% in comparison with the group without biochar. Lipase pretreatment substantially shortened the fermentation time required to complete the anaerobic digestion, from 15 to 9 days. Lipase pretreatment further improved microbial abundance and promoted the enrichment of functional microbes in the anaerobic digestion of FW mediated by biochar. It also changed the methane production pathway from acetotrophic to hydrogenotrophic, thereby ensuring the stability of the anaerobic digestion system in the presence of a high ammonia nitrogen concentration.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2022
·Vol. 17
·期 2
·355-362
·
IF 5.239
Yanqian Gu,Shasha Liu,Gang Wu,Yinlong Wu,Shoujun Zhang,Yong Huang,Shu Zhang,Hong Zhang
Biomass is the sole carbon-containing renewable resource on Earth, and has high potential for the production of platform chemicals. Phenol, one of the most important platform chemicals, is generally derived from lignin conversion. In this study, the production of phenol from co-pyrolysis of cellulose and sodium borohydride at different mass ratios is investigated in detail. The results show that even a small amount of sodium borohydride (cellulose–sodium borohydride = 10:1) could effectively promote the conversion of cellulose to phenol through cellulose-derived levoglucosan intermediates. As the mass ratio of cellulose–sodium borohydride decreased, the relative content of phenol in the products significantly increased, and could be as high as 11.0% when the ratio of cellulose to sodium borohydride was 2:1. Glycosidic bonds in cellulose were considered to play an important role in its catalytic conversion. Furthermore, the resulting solid product could be used as a B-doping carbon material, which is desirable in various research fields. This study provided a novel method for the highly efficient utilization of cellulose. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
研究领域:
生物质能
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化学工程
·
催化转化
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产品关联
DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2022
·Vol. 86
·期 12
·1641-1647
·
IF 2.337
Zhou Yuyang,Mang Rongchen,Du Xuefei,Cai Heng,Zhou Hua
2,5-furanediformate Isooctyl is a potential new green biobased plasticizer. At present, most of the preparation methods are chemical methods, which not only have many by-products and are difficult to separate, but also cause environmental pollution. In this paper, the immobilized lipase Novozym435 was used as the catalyst to catalyze the transesterification of 2,5-furanediformate dimethyl ester and isooctyl alcohol to prepare 2,5-furanediformate isoocty in organic medium, and the reaction process was optimized. The optimal process conditions were determined by single factor experiment: in 10 mL toluene system, the additional amount of immobilized lipase Novozym435 was 0.02 g, the molar ratio of 2,5-furanediformate dimethyl ester (1 mmol) and isooctyl alcohol was 1:4, and 1 g 4Å molecular sieve was added to the reaction system, the reaction temperature was 50°C, the reaction time was 24 h, and the conversion rate of 2,5-furanediformate isoocty was 89.63%.
研究领域:
化学工程
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生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2022
·Vol. 86
·期 9
·1192-1199
·
IF 2.337
Wu Jinhai,Zhao Jiamei,Liu Mingzhu,Zhao Zunquan,Qiu Yu,Li Hanle,Wu Jin,Bai Jialei
We developed a new ochratoxin A (OTA) aptamer biosensor to promptly detect OTA in food. Mesoporous silica nanoparticles were used as carriers, and aptamers were used as recognition probes and gating molecules. The fluorescent dye rhodamine 6G was loaded into mesoporous silica, and through electrostatic contact, the OTA aptamer was adsorbed on amino-modified mesoporous silica. The fluorescent dye released from the mesopore in the presence of OTA because of the conformational change induced in the aptamer by the target. The amount of ochratoxin was determined by measuring the fluorescence intensity. Our findings revealed a positive relationship between the fluorescence intensity and OTA concentration, with a limit of detection of 0.28 ng mL−1, and the detection range was 0.05–200 ng mL−1. The recovery rate was 80.7%–110.8% in real samples. The proposed approach is suitable for the quantification of other toxins.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2023
·Vol. 17
·期 3
·592-601
·
IF 3.9
Liangliang Zhang,Man Xu,Qinhao Guan,Jianchun Jiang,Kang Sun,Emmanuel Manirafasha
Tannin is a chemical compound found in plants such as tea, with yellow or brown as a characteristic color. Tannin is present in any plant species’ wood, stems, barks, leaves, roots, and fruits. However, the tannin contents in those plants are not the same and plants rich in tannin are the most used raw material for industrial-level tannin extraction, such as chestnut. The central purpose of this article was to identify the tannin content of vegetables. In this study, tannins were extracted from 72 plants in China. The contents and purities of tannins, non-tannins and insoluble substances in those studied plants were determined. A gravimetric approach, using hider powder as a precipitate to bind tannins, has been developed to quantify total tannins in plants. The results showed that high contents of tannins (>20%) were found in Pltycarya strobilacea fruit, Quercus acutissima shell, Quercus variabilis shell, Acacia mearnsii bark, Albizia kalkora bark, Camellia oleifera wood, Rosa cymosa root, Rosa laevigata root, Rhus chinensis gallnut, Bruguiera sexangular bark, Kandelia candel bark, Ceriops tagal bark, Lumnitzera racemose bark and Dioscorea cirrhosa tuber. The purity of tannins was more than 70% in some plants: Larix gmelimi bark, Causuarina equisetifolia bark, P. strobilacea fruit, Castanopsis tibetana wood, Quercus aliena shell, Quercus chenii shell and bark, A. mearnsii bark, A. kalkora bark, Bruguiera gymnorrhiza bark, C. tagal bark, K. candel bark and D. cirrhosa tubers . These plants are excellent resources for vegetable tannins in China. © 2023 Society of Industrial Chemistry and John Wiley & Sons Ltd.
研究领域:
植物化学
·
分析化学
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产品关联
DOI
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2023
·Vol. 17
·期 6
·1698-1707
·
IF 3.9
Chao Liu,Zhanghong Xie,Lili Tong,Yuyang Fan,Xiangchen Kong,Rui Xiao
In this study, a sealable fixed-bed reactor was developed to investigate the influence of gaseous products on lignin pyrolysis with a strategy of hydrogen self-supply, and lignin pyrolysis performances at various temperatures were monitored by the comprehensive characterization of the generated products, including gas, bio-oil and char. The results showed that the release of volatiles (both gas and bio-oil) from lignin was strengthened in this sealable system when the temperature rose, and therefore less char was left. Meanwhile no H 2 was detected under any of the operating conditions, which is a common component in pyrolysis gas from conventional reactors, indicating its participation in lignin pyrolysis. Furthermore, the formation of CO and CO 2 in the employed pyrolyzer benefited from not only the thermal evolution of feedstock, but also the interactions between char residues and generated gas components. Meanwhile, because of the hydrogen self-supply, compounds in bio-oil were enriched and only nine species were detected, which were all phenolic compounds. Vanillic acid was dominant, and its relative content was up to 38.86% for Alcell organosolv lignin and 34.41% for soda alkali lignin. This illustrates that highly selective production of phenolic compounds from lignin pyrolysis can be achieved by self-supplying hydrogen from pyrolysis gas.
研究领域:
生物质能
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2023
·
IF 1.6
Guo Cheng,Shen Wangyang,Jin Weiping,Jia Xiwu,Ji Zhili,Jinling Li,Bin Li
Epigallocatechin gallate (EGCG) and caffeine are inevitable to be ingested together in the process of drinking green tea. This study used Caenorhabditis elegans as organism model to examine whether the binding of EGCG and caffeine could influence the fat reduction effect. The results revealed that EGCG significantly reduced the Nile Red fluorescence intensity and the triglyceride/protein ratio of obesity model C. elegans by 14.7% and 16.5% respectively, while the effect of caffeine was not significant. Moreover, the degree of reduction in fluorescence intensity and triglyceride/protein ratio by EGCG + caffeine was comparable to that of EGCG. In the exploration of underlying mechanism, we found that EGCG and EGCG + caffeine treatments had no influence on food intake and energy expenditure of C. elegans. Their fat reduction effects were dependent on the regulation of lipogenesis, as shown by the decreased expression of sbp-1, fat-7 and daf-16 genes.
研究领域:
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）