TOXICOLOGY LETTERS
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2019
·
IF 3.499
Lingmin Zhang,Qingqing Xia,Yingying Zhou,Jie Li
Excessive accumulation of cadmium (Cd) in retina plays an important role in tobacco smoking-associated age-related macular degeneration (AMD). Plenty of evidence has revealed that the retinal pigment epithelium (RPE) is the primary site of pathology in AMD. Our current study demonstrated that Cd induced apoptosis in a human RPE cell line ARPE-19 cells, as it dose-dependently caused cell viability loss and activated caspase-3. The reactive oxygen species (ROS) were confirmed to be important mediators for Cd-triggered cell death in ARPE-19 cells. We found that endoplasmic reticulum (ER) stress was activated as its marker BiP was remarkably upregulated by Cd-exposure. Whereas the antioxidants N-acetylcysteine (NAC) and Tempol significantly suppressed the expression of BiP and CHOP, suggesting that ROS generation is an early trigger of Cd-activated ER stress. Furthermore, we found that Cd-induced oxidative stress significantly increased autophagic flux and p62 expression. A temporal impact of Cd exposure is possibly existed in p62 expression in ARPE-19 cells. Moreover, an ER stress inhibitor salubrinal diminished Cd-induced LC3BII expression and attenuated cytotoxicity, indicating that ER stress mediates autophagy and was implicated in apoptosis of Cd-exposed ARPE-19 cells. However, CHOP expression may not exert impact on the regulation of Cd-caused autophagy. Additionally, inhibition of autophagy with si-Beclin 1 and 3-Methyladenine significantly ameliorated Cd-induced CHOP expression and cytotoxicity, indicating that autophagy was detrimental in Cd-accumulated ARPE-19 cells, and a positive feedback regulation mechanism may exist between Cd-triggered ER stress and autophagy. Taken together, these results suggest that Cd-caused ER stress and autophagy are implicated in RPE cell death associated retinopathies especially related to smoking.
研究领域:
毒理学
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眼科学
·
细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shan Su,Xiaohong Liu,Min Zhu,Wen Liu,Jingyi Liu,Yujia Yuan,Fudong Fu,Zhiyong Rao,Jingping Liu,Yanrong Lu,Younan Chen
Nonalcoholic fatty liver disease (NAFLD), characterized by hepatic lipid deposition, is one of the most prevalent chronic metabolic disorders globally, and its pharmaceutical treatments are still limited. Excessive lipid accumulation triggers endoplasmic reticulum (ER) stress and autophagy flux dysfunction, which are important mechanisms for NAFLD. Trehalose (Tre), a natural disaccharide, has been identified to reduce hepatic steatosis and glucose intolerance. However, its underlying mechanisms for NAFLD remain unclear. In this study, a high-fat-diet (HFD)-induced mouse NAFLD model and a saturated fatty acid palmitic acid (PA)-stimulated cell model were constructed. The results indicated that Tre supplementation ameliorated hepatocyte lipid deposition in vitro, as well as hepatic steatosis and hyperlipidemia in vivo. Mechanistically, Tre alleviated both autophagy flux dysfunction and endoplasmic reticulum (ER) stress. Under the stimulation of HFD or PA, Tre remarkably increased the expression and nucleic translocation of the lysosomal master protein transcription factor EB (TFEB), while decreasing the accumulation of p62 and also decreasing the ER stress markers (inositol-requiring enzyme 1 (IRE1α), XBP-1, CHOP, and BIP). Similar results were observed in an ER stressor tunicamycin (TM)-induced in vivo and in vitro models. In addition, the transcriptomic analysis of NAFLD patients revealed significant differences in ER stress-related and autophagy-related biomarkers, including TFEB, ATG7, IRE1α, and CHOP. Molecular docking results demonstrated a strong affinity between Tre and both IRE1α and TFEB. Overall, Tre protected hepatocytes from lipotoxicity-related ER stress and autophagy dysfunction, and its regulatory effect on the IRE1α–TFEB signaling pathway may be a critical mechanism. These findings suggest that Tre, as a bioactive substance with significant medicinal potential, holds considerable promise for drug development and clinical application in treating NAFLD.
研究领域:
药理学
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分子生物学
·
代谢紊乱
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肝病学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guijiao Zhao,Ying Zhao,Qianqian Wang,Chenhuan Wang,Chun Wang,Zhi Wang,Qiuhua Wu
Endocrine disrupting compounds (EDCs) in food pose severe threats to human wellness, so it is extremely urgent to develop effective method with high sensitivity for detecting EDCs in seafoods. Herein, novel urea-functionalized magnetic porous polymer (M-NB-POP) was synthesized from N,N,N′,N′-tetra( p -aminophenyl)- p -phenylenediamine and 1,3-bis(2-isocyanato-2-propyl)benzene under mild conditions for the first time. M-NB-POP with good recoverability and recyclability shows excellent adsorption for several typical EDCs through hydrogen bonding, π-π and hydrophobic interaction. Using M-NB-POP as magnetic solid-phase extraction adsorbent, an attractive method for effective enrichment of trace EDCs in red shrimp, river prawn and crayfish was successfully developed prior to LC-MS analysis. The proposed method gave a low detection limit of 1.50–7.80 ng g −1 , high spiked recoveries of 86.3–111 %, and small relative standard deviation of 1.6–6.0 %. This work provides a new protocol for assessment of trace EDCs in seafoods and concurrently presents a new strategy for developing functional materials to enrich organic pollutants.
研究领域:
环境科学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
NATURE MATERIALS
·
2025
·
IF 38.5
Liu Huayang,Song Zilin,Zhang Yu,Wu Bihan,Chen Dinghao,Zhou Ziao,Zhang Hongyue,Li Sangshuang,Feng Xinping,Huang Jing,Wang Huaimin
Bioinspired materials based on self-assembling peptides are promising for tackling various challenges in biomedical engineering. While contemporary data-driven approaches have led to the discovery of self-assembling peptides with various structures and properties, predicting the functionalities of these materials is still challenging. Here we describe the deep learning-guided de novo design of antimicrobial materials based on self-assembling peptides targeting bacterial membranes to address the emerging problem of bacterial drug resistance. Our approach integrates non-natural amino acids for enhanced peptide self-assembly and effectively predicts the functional activity of the self-assembling peptide materials with minimal experimental annotation. The designed self-assembling peptide leader displays excellent in vivo therapeutic efficacy against intestinal bacterial infection in mice. Moreover, it exhibits an enhanced biofilm eradication capability and does not induce acquired drug resistance. Mechanistic studies reveal that the designed peptide can self-assemble on bacterial membranes to form nanofibrous structures for killing multidrug-resistant bacteria. This work thus provides a strategy to discover functional peptide materials by customized design.
研究领域:
生物医学工程
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材料科学
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人工智能
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Chunyang Ye,Hao Liang,Kejian Zhang,Bin Ding,Ning Tang,Hongyan Wu
Traditional fibrous warmth retention materials suffer from limited performance improvement due to their micrometer-scale diameter and fail to meet the requirements of lightweight yet high-efficiency cold protection in extreme environments. Herein, we present a novel, facile, and ecofriendly strategy to fabricate a lightweight, mechanically robust nanofibrous sponge with integrated solar-driven active heating and low-temperature superinsulation. The high-porosity structure is achieved through urea-induced phase separation during ambient electrospinning, which overcomes the energy-intensive and unsafe high-humidity processing challenges. Simultaneous in situ incorporation of silicon carbide nanoparticles with photothermal properties enables solar-activated heat generation. This nanofibrous sponge realizes dual functionalities: ultralow thermal conductivity (27.31 mW m–1 K–1) for low-temperature superinsulation and rapid solar heating (50.1 °C temperature rise within 10 min under simulated sunlight), combined with exceptional attributes including lightweight property (volume density of 3.8 mg cm–3), hydrophobicity (water contact angle = 128°), antifouling behavior, and stable mechanical performance. Its superior performance in extreme environments (e.g., high-altitude and polar regions) and medical applications establishes a new paradigm for advanced warmth retention materials with integrated passive/active thermal management functionalities.
研究领域:
材料科学
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能源科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2013
·Vol. 92
·期 4
·720-728
·
IF 1.003
Qingjie Xie,Linzi Li,JianMing Pan,Minjia Meng,Zhilong Song,Wenjing Zhu,Bing Wang,Ping Yu
Molecularly imprinted β-cyclodextrin/Kaoline particles (MIPs) were prepared for recognitive adsorption of bisphenol A (BPA) from aqueous solution. The characterisation of MIPs were achieved by FTIR spectra, SEM micrographs, nitrogen adsorption–desorption analysis and elemental analysis. The equilibrium data, at various temperatures, were described by the Langmuir, Freundlich and Dubinin-Radushkevich isotherm models. The kinetic properties were successfully investigated by pseudo-first-order model, pseudo-second-order model, intraparticle diffusion equation, initial adsorption rate and half-adsorption time. Based on the comparison of diffusion parameters, including intraparticle diffusion, film diffusion and pore diffusion, we can confirm the process of recognitive binging sites in MIPs. A diffusion-controlled process as the essential adsorption rate-controlling step was proposed. Moreover, intraparticle diffusion increased with BPA concentration while film and pore diffusion decreased. An increase in the temperature was found to increase intraparticle and pore diffusion, and reduce film diffusion. The selectivity of MIPs also demonstrated higher affinity for target BPA over competitive phenolic compounds than that of non-imprinted polymers (NIPs).
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Jin Li,Xuebin Zhang,Yuxin Liu,Hongwu Tong,Yeping Xu,Shaomin Liu
In this paper, a highly selective sample cleanup procedure combing dummy molecular imprinting and solid-phase extraction (DMIP–SPE) was developed for the isolation and determination of bisphenol A (BPA) in tap water. The novel hollow porous dummy molecularly imprinted polymer (HPDMIP) was prepared adopting a sacrificial support approach, using tetrabromobisphenol A (TBBPA), whose structure was similar to that of BPA, as the dummy template and mesoporous MCM-48 nanospheres as the support. Owing to a very short distance between the binding sites and the surface, a large surface area and a good steric structure to match its imprint molecules, the maximum adsorption capacities ( Q max ) of the dummy-imprinted and non-imprinted sorbents for BPA were as high as 445 and 340 μmol g –1 , respectively, and the adsorption reached about 73% of Q max in 10 min. Meanwhile, a method was developed for the determination of BPA using HPDMIP as a solid-phase extraction enrichment sorbent coupled with HPLC . Under the optimum experimental conditions, HPDMIP exhibited satisfactory results in the enrichment and determination of BPA in tap water with a recovery rate of 95–105%, and relative standard deviations of below 6%, and it can achieve a limit of detection as low as 3 ng mL −1 . The developed extraction protocol eliminated the effect of template leakage on quantitative analysis and could be applied for the determination of BPA in complicated functional samples.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Hui Ouyang,Limin Wang,Shijia Yang,Wenwen Wang,Lin Wang,Fengquan Liu,Zhifeng Fu
The multianalyte immunoassay (MIA) has attracted increasing attention due to its high sample throughput, short assay time, low sample consumption, and reduced overall cost. However, up to now, the reported MIA methods commonly require multiple antibodies since each antibody can recognize only one antigen. Herein, a novel bispecific monoclonal antibody (BsMcAb) that could bind methyl parathion and imidacloprid simultaneously was produced by a hybrid hybridomas strategy. A chemiluminescence (CL) reaction kinetics-resolved strategy was designed for MIA of methyl parathion and imidacloprid using the BsMcAb as the unique recognition reagent. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were adopted as the signal probes to tag the haptens of the two pesticides due to their very different CL kinetic characteristics. After competitive immunoreactions, the HRP-tagged methyl parathion hapten and the ALP-tagged imidacloprid hapten were simultaneously bound to the BsMcAb since there were two different antigen-binding sites in it. Then, two CL reactions were simultaneously triggered by adding the CL coreactants, and the signals for methyl parathion and imidacloprid detections were collected at 0.6 and 1000 s, respectively. The linear ranges for methyl parathion and imidacloprid were both 1.0–500 ng/mL, with detection limits of 0.33 ng/mL (S/N = 3). The proposed method was successfully used to detect pesticides spiked in ginseng and American ginseng with acceptable recoveries of 80–118%. This proof-of-principle work demonstrated the feasibility of MIA using only one antibody.
研究领域:
分析化学
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生物化学
·
免疫学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Leiliang He,Lihua Ding,Songcheng Yu,Fei Yu,Yongmei Tian,Xinsheng Xie,Lingbo Qu,Lie Liu,Yongjun Wu
The establishment of a highly sensitive and efficient platform for detecting circulating miRNA in serum is important for disease diagnosis and prognosis. To this end, a self-assembled horseradish peroxidase (HRP)-based polymer (poly-HRP) dual signal amplification strategy, via skillful combination of functionalized magnetic nanoparticles as the capture nanoprobes and the poly-HRP as signal amplification probes, has been developed for highl-sensitive and accurate detection of circulating miR-142-3p in human serum. Just by mixing building blocks together at one time, the poly-HRP used for dual signal amplification can be engineered by only one-step assembly. As the poly-HRP can be directly prepared ahead, avoiding chain amplification reaction in the detection process, the assay can be rapidly accomplished in about one hour. The measured limit-of-detection (LOD) is down to 100 fM. Standard miR-142-3p spiked in human serum is measured with ideal recovery rates ranging from 97.46% to 115.90%. More importantly, this method was validated to be applicable to direct detection of miR-142-3p in serums, and the results suggested that the miR-142-3p levels of serum samples obtained from T-cell acute lymphoblastic leukemia children (n = 25) are significantly higher than those from healthy control groups (n = 25) ( P < 0.01). To conclude, the ingenious combination of functionalized magnetic nanoparticles and the poly-HRP presented in this work brings forth a rapid, convenient, yet simple, sensitive, and specific biosensing method that can potentially be applied for clinical diagnosis.
研究领域:
生物化学
·
分子生物学
·
临床诊断
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 183
·期 1
·431-439
·
IF 3.741
Deng Chun,Zhong Yaping,He Yu,Ge Yili,Song Gongwu
We describe a kind of molecularly imprinted polymer (MIP)-based fluorescent sensor. It was obtained by anchoring a surface-imprinted MIP layer for the model analyte bisphenol A (BPA) onto the surface of fluorescent silver nanoclusters (Ag-NCs). The MIP-coated Ag-NCs were synthesized by the sol-gel technique. The hyphenation of the fluorescence of Ag-NCs with MIP technology expands the use of fluorescent nanomaterials in sensing and recognition. BPA is capable of quenching the fluorescence of the Ag-NCs, and the reduction in fluorescence intensity is related to the concentration of BPA in the 0.2 μg mL −1 to 2 mg L −1 range. The detection limit for BPA is 0.02 μg mL −1 , and the recovery of BPA from spiked milk and juice samples ranged from 92.5 to 108.5 %. The MIP-coated Ag-NCs presented here are characterized by their ease of synthesis, selectivity and sensitivity. In our perception, this approach can be extended to numerous other analytes.
A molecularly imprinted polymer (MIP)-based fluorescence assay was developed by anchoring the MIP layer on the surface of silver nanoclusters via a surface molecular imprinting process, with bisphenol A as the model analyte.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 35
·29629-29637
·
IF 3.289
Junlu Sheng,Min Zhang,Wenjing Luo,Jianyong Yu,Bin Ding
Electrospun nanofibrous membranes with thin fiber diameter, small pore size, and high porosity have attracted a great deal of attention in the waterproof and breathable field. However, great challenges still remain in simultaneously reinforcing the mechanical and waterproof-breathable performance of such materials. In this study, a new type of fluorinated polyurethane/polyacrylonitrile/polyvinyl butyral nanofibrous membranes (FPU/PAN/PVB NFM) with a blocked isocyanate prepolymer (BIP) as chemical cross-linking agent were fabricated. The composite NFM, with robust mechanical, waterproof and breathable performance, has been prepared using an innovation strategy combining electrospinning with thermally induced physical bonding and chemical cross-linking. By systematically tuning the cross-linked temperature and time as well as the concentration of PVB and BIP, large breaking elongation (81.5%), good hydrostatic pressure (110 kPa) and modest WVTR (9.6 kg m−2 d−1) of the membranes were achieved. Meanwhile, the physically bonded structure and chemically cross-linked networks between PVB and BIP endowed the composite NFM with the robust tensile strength of 32.8 MPa, which was three times higher than that of pristine FPU/PAN membranes. Considering the excellent performance of the as-prepared membranes, this simple and intriguing approach may provide a versatile platform for exploring the applications of the bonded and cross-linked membranes in separation processes, membrane distillation, self-cleaning materials, and protective clothing.
研究领域:
材料科学
·
纺织工程
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Hangbo Xu,Cao Fang,Changsheng Shao,Lamei Li,Qing Huang
Cold atmospheric plasma (CAP) possesses the ability of high-efficiency disinfection. It is reported that mixtures of reactive oxygen species (ROS) including · OH, 1 O 2 , O 2 − and H 2 O 2 generated from CAP have better antimicrobial ability than mimicked solution of mixture of single ROS type, but the reason is not clear. In this study, CAP was applied to treat yeasts in water in order to investigate the fungal inactivation efficiency and mechanism. The results showed that plasma treatment for 5 min could result in >2-log reduction of yeast cells, and application of varied ROS scavengers could significantly increase the yeast survival rate, indicating that · OH and 1 O 2 played the pivotal role in yeast inactivation. Moreover, the synergistic effect of 1 O 2 with other plasma-generated ROS was revealed. 1 O 2 could diffuse into cells and induce the depolarization of mitochondrial membrane potential (MMP), and different levels of MMP depolarization determined different cell death modes. Mild damage of mitochondria during short-term plasma treatment could lead to apoptosis. For long-term plasma treatment, the cell membrane could be severely damaged by the plasma-generated · OH, so a large amount of 1 O 2 could induce more depolarization of MMP, leading to increase of intracellular O 2 − and Fe 2+ which subsequently caused cell inactivation. 1 O 2 could also induce protein aggregation and increase of RIP1/RIP3 necrosome, leading to necroptosis. With participation of 1 O 2 , endogenous · OH could also be generated via Fenton and Haber-Weiss reactions during plasma treatment, which potentiated necroptosis. Adding l -His could mitigate membrane damage, inhibit the drop of MMP and the formation of necrosome, and thus prevent the happening of necroptosis. These findings may deepen the understanding of plasma sterilization mechanisms and provide guidance for microbial killing in the environment.
研究领域:
环境科学
·
微生物学
·
等离子体物理
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）