SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaopeng Fan,Dan Zhang,Hongjuan Li,Shiguo Sun,Yongqian Xu
A novel near infrared (NIR) fluorescent hybrid system ( PD-SQ ⊂ BSA ) assembled from squaraine dye PD-SQ and bovine serum albumin (BSA) was developed for selective detection of Ag + over other metal ions in absolute PBS. The detection limit of PD-SQ ⊂ BSA for Ag + is calculated to be about 8.99 × 10 −8 M, which is lower than the guidelines for drinking water set by the WHO. PD-SQ alone shows very weak fluorescence due to aggregation-caused quenching (ACQ) property. BSA can cause PD-SQ to partially disaggregate through electrostatic and hydrophobic interactions, but various metal ions including Ag + cannot do. Interestingly, the presence of both BSA and Ag + can trigger PD-SQ to almost completely disaggregate through synergic binding of multiple S-Ag linkings derived from the biothiol moieties in BSA and PD-SQ with Ag + , resulting in obvious turn-on fluorescence change. Absorption and field emission scanning electron microscope (FESEM) spectra have been used to prove the conversion of PD-SQ from aggregates to monomers. In addition, based on the fluorescence spectra changes of PD-SQ ⊂ BSA system upon addition of Hg 2+ and ethylenediaminetetraacetic acid (EDTA), logic circuits including AND and INHIBIT logic gates with reset function were successfully constructed. This type of fluorescent system shows great potential for disease diagnosis, environmental protection, and information protection at the molecular scale.
研究领域:
生物化学
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环境科学
·
分子生物学
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DOI
高影响因子
Journal of Environmental Sciences
·
2022
·
IF 6.796
Wenjing Tang,Yunyi Meng,Bin Yang,Dongyu He,Yan Li,Bojun Li,Zheming Shi,Changwei Zhao
Nanofiltration (NF) process has become one of the most promising technologies to remove micro-organic combined water pollution . Developing a NF membrane material with efficient separation for perfluorooctanoic acid (PFOA) combined pollution is highly desired, this manuscript targets this unmet need specifically. In this work, hydrophilic SiO 2 nanoparticles with various contents blended with carboxylic multiwalled carbon nanotube were used to modify poly (m-phenylene isophthal amide) (SiO 2 /CMWCNT/PMIA) hollow fiber NF membrane. The modified membrane with 0.1 wt% SiO 2 doping exhibits way better fouling resistance with irreversible fouling ratio decreased dramatically from 18.7% to 2.3%, and the recovery rate of water flux increases significantly from 81.2% to 97.7%. The separation experiment results had confirmed that the modified membrane could improve the rejection from 97.2% to 98.6% for perfluorooctanoic acid (PFOA) and its combined pollution with bovine serum albumin (BSA). It is clear that this reported SiO 2 /CMWCNT/PMIA hollow fiber NF membrane potentially could be applied in water treatment . This research also provides a theoretical basis for efficiently removal of PFOA and its combined pollution by NF membrane.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2019
·Vol. 19
·期 12
·2824
·
IF 3.031
Zhikuang Dai,Ailing Yang,Xichang Bao,Renqiang Yang
Transition-metal nanomaterials are very important to non-enzymatic glucose sensing because of their excellent electrocatalytic ability, good selectivity, the fact that they are not easily interfered with by chloride ion (Cl−), and low cost. However, the linear detection range needs to be expanded. In this paper, Cu2O–bovine serum albumin (BSA) core-shell nanoparticles (NPs) were synthesized for the first time in air at room temperature by a facile and green route. The structure and morphology of Cu2O–BSA NPs were characterized. The as-prepared Cu2O–BSA NPs were used to modify the glassy carbon electrode (GCE) in a Nafion matrix. By using cyclic voltammetry (CV), the influence from scanning speed, concentration of NaOH, and load of Cu2O–BSA NPs for the modified electrodes was probed. Cu2O–BSA NPs showed direct electrocatalytic activity for the oxidation of glucose in 50 mM NaOH solution at 0.6 V. The chronoamperometry result showed this constructing sensor in the detection of glucose with a lowest detection limit of 0.4 μM, a linear detection range up to 10 mM, a high sensitivity of 1144.81 μAmM−1cm−2and reliable anti-interference property to Cl−, uric acid (UA), ascorbic acid (AA), and acetaminophen (AP). Cu2O–BSA NPs are promising nanostructures for the fabrication of non-enzymatic glucose electrochemical sensing devices.Keywords:Cu2O–BSA nanoparticles;non-enzymatic glucose sensing;cyclic voltammetry;chronoamperometry;anti-interference
研究领域:
生物化学
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材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Nannan Zheng,Yitong Chen,Lei Jiang,Hongchao Ma
Combined photo-induced cancer therapy (photothermal therapy (PTT) and photodynamic therapy (PDT)) is a promising anti-cancer strategy for its non-invasiveness property. However, severe hypoxia in tumor microenvironment (TME) would weaken the PDT effect and limit the clinical efficiency of PTT/PDT treatment. To solve this problem, denatured BSA-hemin-IR780 (dBHI) was designed and synthesized for anti-cancer treatment in this work. Denatured bovine serum albumin (dBSA) was used as the binding matrix to bind hemin and IR780 via covalent reaction and hydrophobic self-assembly, respectively. The obtained dBHI nanoparticles possess both PTT and PDT effect due to the presence of IR780 in the composites. Moreover, because of the existence of hemin, dBHI also exhibited photo-enhanced Fenton-like catalytic performance, which could decompose H 2 O 2 to form oxygen for improving the PDT effect and generate hydroxyl radical (·OH) for chemodynamic therapy (CDT). Therefore, by using dBHI, synergistic effect of PTT/PDT/CDT combined treatment has been achieved for cancer therapy.
研究领域:
癌症治疗
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纳米技术
·
光疗
·
化学动力学治疗
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2013
·Vol. 37
·期 4
·419-426
·
IF 2.591
Cenjin Zhang,Xiaoping Jia,Yuzhi Wang,Min Zhang,Shan Yang,Junxia Guo
A novel temperature-sensitive molecularly imprinted hydrogel composed of N -isopropylacrylamide and acrylamide has been prepared by using free-radical polymerization and was cross-linked by modified water-soluble N -maley chitosan in aqueous solution. BSA (pI 4.9, MW 66.0 kDa) was used as the template protein. The produced hydrogels were characterized by environmental SEM to reveal the microcosmic morphology. A microporous structure was only found in the imprinted hydrogel, while no obvious microporous structure was found in nonimprinted hydrogels. The lower critical solution temperature of the hydrogels was 34°C, and the optimal binding conditions were tested, namely, the adsorption equilibrium time of 6 h and initial BSA concentration of 1.0 mg/mL. The adsorption capacity Q max was determined by Langmuir isotherm plots and was 5.72 mg/g for imprinted hydrogel and 1.18 mg/g for nonimprinted hydrogels. A separation factor (β) of 4 was obtained when bovine hemoglobin (pI 6.9, MW 64.0 kDa) was selected as the particular reference protein. Molecular weights and pIs were chosen to investigate the selectivity of the hydrogels. It was shown that the shape memory and the size effect were the major factors for the recognition. This imprinted hydrogel was used to specifically adsorb the BSA from the protein mixture.
研究领域:
生物材料学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2017
·Vol. 32
·期 7
·1277-1282
·
IF 1.509
Keying Cai,Mulan Zeng,Fenfen Liu,Nan Liu,Zhenzhong Huang,Yonghai Song,Li Wang
An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA–AuNPs@Tb–AMP (BSA, bovine serum albumin; AMP, adenosine 5′-monophosphate; AuNPs, Au nanoparticles) metal–organic framework (MOF) nanostructures were synthesized by encapsulating BSA–AuNPs into Tb–AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg 2+ . DPA could strongly co-ordinate with Tb 3+ to replace water molecules from the Tb 3+ center and accordingly enhanced the fluorescence of Tb–AMP MOFs. The fluorescence of BSA–AuNPs at 405 nm remained constant. While the fluorescence of BSA–AuNPs at 635 nm was quenched after Hg 2+ was added, the fluorescence of Tb–AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg 2+ . The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F 545 /F 405 linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F 635 /F 405 increased linearly with increase of Hg 2+ concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg 2+ in running water.
研究领域:
材料科学
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 19
·4552-4558
·
IF 4.23
Xing Liu,Qi Wang,Huihui Zhao,Lichun Zhang,Yingying Su,Yi Lv
Inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of pH and temperature and lower sensitivity to proteases. Although many artificial enzymes have been investigated, searching for highly-efficient and stable catalysts is still of great interest. In this paper, we first demonstrated that bovine serum albumin (BSA)-stabilized MnO2 nanoparticles (NPs) exhibited highly peroxidase-, oxidase-, and catalase-like activities. The activities of the BSA-MnO2 NPs were evaluated using the typical horseradish peroxidase (HRP) substrates o-phenylenediamine (OPD) and 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of either hydrogen peroxide or dissolved oxygen. These small-sized BSA-MnO2 NPs with good dispersion, solubility and biocompatibility exhibited typical Michaelis–Menten kinetics and high affinity for H2O2, OPD and TMB, indicating that BSA-MnO2 NPs can be used as satisfactory enzyme mimics. Based on these findings, BSA-MnO2 NPs were used as colorimetric immunoassay tags for the detection of goat anti-human IgG in place of HRP. The colorimetric immunoassay using BSA-MnO2 NPs has the advantages of being fast, robust, inexpensive, easily prepared and with no HRP and H2O2 being needed. These water-soluble BSA-MnO2 NPs may have promising potential applications in biotechnology, bioassays, and biomedicine.
研究领域:
材料科学
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生物化学
·
生物技术
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2022
·Vol. 18
·期 16
·3157-3167
·
IF 4.046
Zhouxuan Xiang,Ting Liu,Huimin Wang,Genxin Chen,Xiongbin Zhu,Tonghui Hao,Jiabing Ran,Changying Yang
Developing customizable pH-responsiveness for supramolecular hydrogels is of great significance and has drawn tremendous attention. Through systematic simulation analysis, we formulated a simple supramolecular hydrogel (i.e., poly(AAm-co-NaSS)/BSA on the basis of electrostatic interaction between the sulfonate groups of poly(AAm-co-NaSS) and the protonated side groups of BSA, and proposed a novel pH-responsive mode for it: changing the internal electric charge composition of the hydrogel through pH-induced ionization/protonation transition of BSA, thereby regulating the structural stability/shrinkage/extension of the supramolecular network. On basis of this theory, the pH-responsiveness of the poly(AAm-co-NaSS)/BSA hydrogel, in principle, could be pre-designed by adjusting the initial BSA/NaSS ratio. In this regard, we fabricated a poly(AAm-co-NaSS)/BSA hydrogel prototype with a BSA/NaSS ratio of 1/57 and investigated its rheological/swelling/disassembling behavior under different pH conditions (1.7, 4.7, 7.7, 10.7, and 13.7). In addition, we also prepared two capecitabine-loaded poly(AAm-co-NaSS)/BSA hydrogel prototypes with BSA/NaSS ratios of 1/57 and 1/102 respectively at pH 4.0, and compared their drug release behavior in SGF and SIF. Finally, the experimental results fitted well with our theoretical expectations, which testified the rationality of our assumption. Thus, we believed that the poly(AAm-co-NaSS)/BSA supramolecular hydrogel could find diverse applications in the future.
研究领域:
生物材料学
·
药物递送系统
·
高分子化学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2017
·Vol. 33
·期 1
·119-124
·
IF 1.509
Nan Liu,Juan Hao,Keying Cai,Mulan Zeng,Zhenzhong Huang,Lili Chen,Bingxian Peng,Ping Li,Li Wang,Yonghai Song
A novel ratiometric fluorescence nanosensor for superoxide anion (O 2 •− ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb 3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O 2 •− . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O 2 •− demonstrated high sensitivity and selectivity with a wide linear response range (14 nM–10 μM) and a low detection limit (4.7 nM).
研究领域:
分析化学
·
纳米技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-8
·
IF 5.7
Huang Feng,Deng Lei,Wang Tingting,Zhang Aomei,Yang Minghui,Hou Yi
A bovine serum albumin (BSA)-assisted ultrasonication strategy was developed for the synthesis of CsPbBr 3 nanocrystals (NCs) with stable fluorescence properties in aqueous solution. Such a preparation method is simple, fast and does not require complex equipment. The results show that the synthesized CsPbBr 3 NCs are homogeneous in particle size and have good solubility and stability in water. The CsPbBr 3 NCs have been utilized as fluorescence probe for rapid detection of hydrogen sulfide (H 2 S) in human serum. The reaction of H 2 S with the lead sites on the surface of CsPbBr 3 NCs produces lead sulfide (PbS), resulting in the decrease of fluorometric intensity of CsPbBr 3 NCs. Our designed fluorescent assay has a linear S 2− detecting range of 10 ~ 800 nM with a detection limit of 7.05 nM. The assay was used to determine H 2 S in human serum with spiked recoveries ranging from 94.98% to 102.69%. This work opens new avenues for the application of halide lead perovskite in different biosensing areas. Graphical abstract
研究领域:
纳米技术
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Rui Zhu,Yan Zhou,Xi-Liang Wang,Li-Ping Liang,Yi-Juan Long,Qin-Long Wang,Hai-Jie Zhang,Xiao-Xiao Huang,Hu-Zhi Zheng
It was found that Hg 2+ can inhibit the peroxidase mimetic activity of bovine serum albumin (BSA) protected Au clusters (BSA-Au) due to the specific interaction between Hg 2+ and Au + existed onto the surface of BSA-Au clusters. By coupling with 3, 3′, 5, 5′-tetramethylbenzidine (TMB)–H 2 O 2 chromogenic reaction, a novel method for Hg 2+ detection was developed based on the inhibiting effect of Hg 2+ on BSA-Au clusters peroxidase-like activity. This method exhibited high selectivity and sensitivity. As low as 3 nM (0.6 ppb, 3 σ ) Hg 2+ could be detected with a linear range from 10 nM (2 ppb) to 10 µM (2 ppm) and this method was successfully applied for the determination of total mercury content in skin lightening products.
研究领域:
分析化学
·
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Xin Liu,Shiyue Niu,Bin Yang,Jia Liu,Liqian Niu,Xian Wang,Daqian Song,Shuyun Bi
Ultrasensitive Surface-enhanced Raman spectroscopy (SERS) method for the detection of diquat/dipterex was established using bovine serum albumin (BSA)-protected silver nanoparticles (AgNPs) modified MIL-53(Al) (named as BSA/MIL-53(Al)/AgNPs). Compared with unmodified AgNPs, BSA/MIL-53(Al)/AgNPs significantly enhanced the Raman signals of diquat and dipterex and the enhancement factors (EFs) were 1.58 × 10 7 and 2.34 × 10 7 , respectively. The TEM, XRD, TGA, XPS, UV–vis and FT-IR were utilized to characterize BSA/MIL-53(Al)/AgNPs and the binding of the substrate with diquat/dipterex. The optimal measurement conditions were investigated in detail by single factor experiment and response surface model. The impacts of common pesticides and coexisting substances on the determination of diquat/dipterex were studied. Under optimum conditions, linear calibration curves for detecting diquat/dipterex were established with a limit of detection (LOD) of 0.17/0.89 pmol L −1 (3 S 0 / S ). The SERS approaches were used to detect diquat and dipterex in several fruits and vegetables. The recovery was 97.10 %–104.82 % with the relative standard deviation (RSD) of 1.04 %–4.15 % ( n = 5).
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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