FREE RADICAL BIOLOGY AND MEDICINE
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2026
·
IF 8.2
Xinyi Zeng,Deijian Peng,Yunlong Shen,Li Tang,Tianlu Ran,Ziheng Pan,Hui Liu
Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by the abnormal aggregation of amyloid-β (Aβ). Bletilla striata polysaccharide (BSP), the primary active component of the traditional Chinese medicine Bletilla striata , exhibits various pharmacological effects including hemostatic, antioxidant, anti-inflammatory, and immunomodulatory activities. This study aimed to systematically investigate the protective effects and molecular mechanisms of BSP in Caenorhabditis elegans AD model. We found that BSP effectively alleviated the paralysis phenotype in AD worms, with optimal efficacy observed at a concentration of 100 μg/mL. Furthermore, BSP significantly extended the lifespan of both wild type and AD worms, reduced lipofuscin deposition and egg-laying capacity, improved neuromuscular function, learning ability, and stress resistance, and lowered the level of oxidative stress in vivo. Additionally, BSP treatment markedly suppressed Aβ aggregation in AD worms.Transcriptomic analysis revealed that BSP significantly regulates the autophagy pathway. In combination with genetic experiments, we further elucidated that BSP coordinates the insulin and AMPK signaling pathways to modulate autophagy, thereby reducing abnormal autophagosome accumulation and restoring autophagic homeostasis. Notably, the neuroprotective effects of BSP were completely abolished in mutants of key insulin signaling pathway genes ( daf-2 , age-1 , akt-1 , akt-2 , daf-16 ) and the AMPK homologous gene aak-2 , indicating that its efficacy is associated with the insulin/AMPK-autophagy regulatory axis. This study reveals the mechanism by which BSP ameliorates AD pathology through multi-target and multi-pathway regulation of autophagy, providing a new theoretical basis for its development as a candidate therapeutic agent for AD and further highlighting the potential medical value of Bletilla striata in combating AD.
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Biomaterials Science
·
2026
·
IF 5.7
Shan Yu,Ling Wang,Zhenbo Peng,Yanjun Chen,Peifang Xu
Chronic diabetic wounds remain difficult to heal due to persistent inflammation and impaired macrophage polarization. Herein, we developed an immunoregulatory dressing using a hybrid hydrogel (C-B-S) synthesized from natural polysaccharides through the photo-polymerization of carboxymethyl chitosan methacrylate (CMCMA), Bletilla striata polysaccharide methacrylate (BSPMA), and thiolated hyaluronic acid (HA-SH). The hydrogel demonstrated dual functionality by promoting hemostasis and inducing macrophage polarization toward the M2 phenotype. RNA sequencing analysis revealed that the immunomodulatory effect of Bletilla striata polysaccharide is mediated via the PPAR signaling pathway. In vivo studies demonstrated the C-B-S hydrogel enhanced angiogenesis, re-epithelialization, and collagen deposition, leading to the fastest wound closure among all groups. This study offers a transformative therapeutic strategy to restore immune balance and accelerate diabetic tissue repair, highlighting its potential for clinical translation in treating chronic diabetic wounds.
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CARBOHYDRATE POLYMERS
·
2023
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IF 11.2
Guanghui Jiang,Bulei Wang,Yuwen Wang,Haoyue Kong,Yufei Wang,Peng Gao,Minghao Guo,Wenna Li,Jian Zhang,Zhezhi Wang,Junfeng Niu
Liver fibrosis has proven to be the main predisposing factor for liver cirrhosis and liver cancer; however, an effective treatment remains elusive. Polysaccharides , with low toxicity and a wide range of bioactivities, are strong potential candidates for anti-hepatic fibrosis applications. For this study, a new low molecular weight neutral polysaccharide ( B. striata glucomannan (BSP)) was extracted and purified from Bletilla striata . The structure of BSP was characterized and its activities for alleviating liver fibrosis in vivo were further evaluated. The results revealed that the structural unit of BSP was likely →4)-β-D-Glc p -(1 → 4)-β-D-Man p -(1 → 4)-β-D-2ace-Man p -(1 → 4)-β-D-Man p -(1 → 4)-β-D-Glc p -(1 → 4)-β-D-Man p -(1 → 4)-β-D-Man p -(1 → 4)-β-D-3ace-Man p -(1→, with a molecular weight of only 58.5 kDa. Additionally, BSP was observed to attenuate the passive impacts of liver fibrosis in a manner closely related to TLR2/TLR4-MyD88-NF-κB signaling pathway conduction. In summary, the results of this study provide theoretical foundations for the potential applications of BSP as an anti-liver fibrosis platform.
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JOURNAL OF PHARMACY AND PHARMACOLOGY
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2018
·Vol. 70
·期 6
·797-807
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IF 2.309
Zhao Lichun,Sun Dandan,Lu Haibin,Han Bing,Zhang Guangyuan,Guan Qingxiang
ObjectivesA system of stearic acid (SA)-modified Bletilla striata polysaccharide (BSP) micelles was developed for the targeted delivery of docetaxel (DTX) as a model anticancer drug (DTX-SA-BSP).MethodsParticle size, zeta potential and DTX release in vitro were measured in release media at different pH values. Quantitative cellular uptake, cytotoxicity assay in vitro and antitumour efficacy in vivo were also evaluated. Cell apoptosis was assessed by flow cytometry.Key findingsDTX-SA-BSP copolymer micelles displayed pH-dependent properties in the respects of particle size, zeta potential and in vitro release behaviour ranging from pH 5.0 to pH 7.4. DTX-SA-BSP copolymer micelles showed higher release rate at pH 5.0 than that at pH 6.0 and 7.4. In vitro cytotoxic effect of DTX-SA-BSP copolymer micelles was higher than that of DTX injection. The results of high-performance liquid chromatography determination confirmed that DTX cellular uptake of micelles was enhanced compared with that of DTX injection. Anticancer activity in vivo further confirmed the enhanced tumour targeting and anticancer efficacy of DTX-SA-BSP copolymer micelles.ConclusionsThe above results show that DTX-SA-BSP copolymer micelles have pH sensitivity. SA-BSP copolymers are a promising carrier for delivering hydrophobic anticancer drugs.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2024
·Vol. 25
·期 19
·10563
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IF 4.9
Fei Ran,Kailang Mu,Gang Liu,Yuchen Liu,Yuxin Pang,Guo Feng,Lingli Zhou,Leqiang Peng
Plant-derived medicinal materials have significant potential and promising applications in wound healing and skin regeneration. This study aims to develop a plant-based extract hydrogel from Bletilla striata (Thunb.Reichb.f.), specifically a glucosyloxybenzyl 2-isobutylmalates extract (B), and characterize its potential effects on wound healing. We synthesized the hydrogel using carbomer (C), glycerol (G), and triethanolamine (T) as the matrix, incorporating B into the hydrogel base, and evaluated its physical and chemical properties. In vitro tests assessed the biocompatibility of the glucosyloxybenzyl 2-isobutylmalates-carbomer-glycerol-triethanolamine (B-CGT) hydrogel and its effects on cell proliferation, migration, and adhesion. Animal model experiments evaluated its potential to promote wound healing. The results showed that the prepared B-CGT hydrogel possessed a good three-dimensional network structure and stability, demonstrating significant free radical scavenging capacity in antioxidant tests. In cell experiments, the B-CGT hydrogel exhibited no potential cytotoxicity and showed good hemocompatibility and promotion of cell proliferation. Animal experiments indicated that wounds treated with the B-CGT hydrogel healed significantly faster, with improved formation of new epithelial tissue and collagen. This study suggests that the developed B-CGT hydrogel is a promising candidate for wound dressings, with excellent physicochemical properties and controlled drug release capabilities, effectively promoting the wound healing process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yuanhui Lu,Qian Peng,Guopeng Tao,Youjue Fu,Yujie He,Qiuyan Li,Yin Sun,Ping Zhou,Dawa Zhuoma,Kaijun Gou,Rui Zeng,Xiao Wang
A multifunctional self-healing hydrogel based on dynamic borate ester bonds was developed for diabetic infected wounds. • Humic acid endowed the hydrogel with near-infrared-triggered photothermal antibacterial and potent ROS-scavenging capabilities. • The hydrogel effectively modulated the immune microenvironment by promoting RAW264.7 polarization toward the pro-healing M2 phenotype. The characteristics of diabetic wounds include persistent oxidative stress, chronic inflammation, and impaired tissue regeneration, making effective treatment challenging. A multifunctional self-healing hydrogel was developed by co -grafting Bletilla striata polysaccharide (BSP) with 3-aminophenylboronic acid and polyvinyl alcohol (PVA) via dynamic borate ester bonds. The resulting CBPP/PVA@HAs gel exhibits rapid self-healing properties, sprayability, tissue adhesiveness, and good biocompatibility. In vitro studies demonstrated that the gel showed efficient photothermal antibacterial activity against E. coli , S. aureus , and MRSA, effectively scavenged intracellular reactive oxygen species (ROS), and promoted the polarization of RAW264.7 macrophages toward the anti-inflammatory M2 phenotype. In vivo experiments further demonstrated that the hydrogel significantly accelerated diabetic wound healing, promoted collagen deposition, angiogenesis, and tissue regeneration. Mechanistically, the hydrogel activated the Nrf2/HO-1 antioxidant pathway, reduced the pro-inflammatory cytokines TNF-α and IL-1β, and increased anti-inflammatory cytokines (IL-10) and reparative factors (TGF-β). Consequently, the hydrogel improved the inflammatory microenvironment by alleviating oxidative stress. Overall, CBPP/PVA@HAs gel represents a promising therapeutic strategy for diabetic wounds by simultaneously regulating oxidative stress and inflammation. Schematic diagram illustrating the mechanism of action of the hydrogel in repairing diabetic infected wounds. Download: Download high-res image (121KB) Download: Download full-size image
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Nan Wang,Xiaoyong Tian,Baijie Cheng,Shanyi Guang,Hongyao Xu
Biodegradable natural polymers are receiving increasing attention as potential candidates for wound dressing. In the present study, composite microspheres (mCSB) based on calcium alginate (CA), silk fibroin peptide (SP), and Bletilla striata polysaccharide (BSP) were prepared by the reverse emulsion method. The excellent swelling properties of microspheres enable them to rapidly promote thrombosis. Microspheres can increase the platelet aggregation index to 1.5 and the aggregation rate of red blood cells to as high as 80 %. Furthermore, tannic acid (TA)-loaded microspheres demonstrate a slow-release effect on TA; this allows the microspheres to exhibit good long-lasting antibacterial properties . Due to the synergistic effects of SP and TA, the cell senescence was delayed, with a 126.69 % survival rate of fibroblasts after 3 days of incubation. In addition, TA led to a rapid reduction in inflammation levels, with a wound closure rate of >92.80 % within 7 days. The multifunctional TA-loaded mCSB has great application potential for rapid wound healing and the treatment of wound hemostasis .
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BMC Complementary Medicine and Therapies
·
2022
·Vol. 22
·期 1
·1-14
·
IF 2.838
Luo Yiyuan,Wang Juan,Li Shuo,Wu Yue,Wang Zhirui,Chen Shaojun,Chen Hongjiang
Background Bletilla striata is the main medicine of many skin whitening classic formulas in traditional Chinese medicine (TCM) and is widely used in cosmetic industry recently. However, its active ingredients are still unclear and its fibrous roots are not used effectively. The aim of the present study is to discover and identify its potential anti-melanogenic active constituents by zebrafish model and molecular docking. Methods The antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2′-azino-bis-(3-ethylbenthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay . The anti-melanogenic activity was assessed by tyrosinase inhibitory activity in vitro and melanin inhibitory in zebrafish. The chemical profiles were performed by ultra-high-performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Meanwhile, the potential anti-melanogenic active constituents were temporary identified by molecular docking. Results The 95% ethanol extract of B. striata fibrous roots (EFB) possessed the strongest DPPH, ABTS, FRAP and tyrosinase inhibitory activities, with IC 50 5.94 mg/L, 11.69 mg/L, 6.92 mmol FeSO 4 /g, and 58.92 mg/L, respectively. In addition, EFB and 95% ethanol extract of B. striata tuber (ETB) significantly reduced the melanin synthesis of zebrafish embryos in a dose-dependent manner. 39 chemical compositions, including 24 stilbenoids were tentatively identified from EFB and ETB. Molecular docking indicated that there were 83 (including 60 stilbenoids) and 85 (including 70 stilbenoids) compounds exhibited stronger binding affinities toward tyrosinase and adenylate cyclase. Conclusion The present findings supported the rationale for the use of EFB and ETB as natural skin-whitening agents in pharmaceutical and cosmetic industries.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Lingchong Wang,Yujia Wu,Junsong Li,Hongzhi Qiao,Liuqing Di
This study described the rheological and mucoadhesive properties of one natural water-soluble polysaccharide from Bletilla striata (BSP). The rheological characteristics of BSP in aqueous solutions and BSP mixed with other polymers were investigated under various conditions, including concentration, temperature, pH, and salt addition. Viscometric studies and ex vivo mucoadhesion tests were also conducted to examine the mucoadhesive properties of BSP. Results indicated that BSP behaved as a shear-thinning fluid at various concentrations, and its viscosity decreased at high temperatures. The viscous flow properties of the BSP mixtures changed at high pH (>5.0). Conversely, the viscosity of the BSP solutions was slightly affected by electrolytes. The viscosities of the BSP mixtures with four other commonly used polymers (sodium alginate, sodium carboxymethyl cellulose, hypromellose , and chitosan) were enhanced. The synergistic viscosity of BSP/mucin mixtures increased as BSP concentrations increased, and the maximum value was observed in the SIF medium without enzymes . The adhesive abilities of 5.0% and 10.0% BSP were almost equivalent to that of 0.5% sodium alginate , suggesting that BSP exhibited a certain mucoadhesive property, although it was weaker than that of the other commonly used mucoadhesive materials. BSP showed potential for pharmaceutical excipient applications in bioadhesive drug delivery systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xialin Sun,Tingwen Zhang,Yan Zhao,Han Yang,Yawei Li,Xin Sun,Xingbo Bian
Background Ulcerative colitis (UC) involves intestinal barrier dysfunction, immune dysregulation, and microbiota imbalance. Bletilla striata polysaccharide (BSP) shows anti-inflammatory potential, but its UC mechanisms remain unclear. Purpose To purify Bletilla striata polysaccharide (BSP-1), characterize its structure, and elucidate its multi-target UC therapeutic mechanisms. Methods This study purified a novel structure of neutral glucomannan with a small amount of acetylation (BSP-1). Together with tight junction protein analysis, oxidative stress markers, 16S rRNA sequencing, cytokine quantification, and molecular ecological network examination, the team used a UC mouse model created using dextran sodium sulfate (DSS). Results BSP-1 alleviated colitis by inhibiting TLR4/MyD88/NF-κB signaling (elevating IL-10; lowering IL-6, TNF-α, and IL-1β) and restoring integrity of the intestinal barrier via upregulating Occludin, ZO-1, MUC2, and Claudin-1. It reduced oxidative stress (lower MDA; higher SOD) and modulated gut microbiota (enriched Lactobacillus , suppressed Escherichia - Shigella ). Molecular ecological network analysis showed that BSP-1 restored microbial community stability by enhancing bacterial competition. Conclusion BSP-1 exerts integrated anti-inflammatory, barrier-repairing, and microbiota-regulating effects, highlighting its potential as a multi-target UC therapy and intestinal microecological regulator.
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JOURNAL OF POROUS MATERIALS
·
2022
·Vol. 29
·期 4
·991-1000
·
IF 2.523
Ru Xuanwen,Zhao Kai,Hu Zhengbo,Lu Yan,Jia Xiwen,Ding Zhishan,Zhu Bingqi
In this study, Bletilla striata polysaccharide/powdered activated carbon (BSP/PAC) aerogels were prepared by freeze-drying of homogeneous mixture of BSP and PAC. Solvent replacement method, mechanical analysis, scanning electron microscope and Fourier transform infrared spectroscopy were used to characterize the morphology and performance of the aerogels. Dyes including anionic methylene orange (MO), cationic methylene blue (MB) and oil red O (ORO) and oils including trichloromethane, n-hexane, edible oil and engine oil in aqueous solution were used to study the adsorption properties of aerogels. The results showed that the prepared aerogels had porous structure and good adsorption capacity. The results of dye adsorption showed that BSP/PAC aerogel had stronger adsorption capacity for MB compared with MO and ORO, and the adsorption data for MB were more consistent with the Langmuir model with maximum experimental capacity of 166.295 mg·g − 1 , indicating the efficiency in absorption. In addition, according to the results of adsorption kinetics, the adsorption of MB on BSP/PAC aerogels could be described by a quasi-second-order model. The results of oil adsorption showed that BSP/PAC had the highest adsorption rate of edible oil compared with the other oils, which was nearly 52 times of its own weight. Overall, BSP/PAC is a porous material with three-dimensional structure, which can effectively remove MB dye and oil from water, and may have potential application prospects in sewage purification.
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Frontiers in Plant Science
·
2023
·
IF 5.6
Pengfei Deng,Ruoyong Yin,Huiling Wang,Leiru Chen,Xiaoqing Cao,Xiaoniu Xu
The intercropping practice has been regarded as a practical land-use selection to improve the management benefits of Bletilla striata plantations. The reports about the variety of economic and functional traits of Bletilla pseudobulb under intercropping systems were limited. The present study investigated the variation of economic and functional traits of Bletilla pseudobulb under different intercropping systems (the deep-rooted intercropping system: B. striata - Cyclocarya paliurus, CB; and the shallow-rooted intercropping system: B. striata - Phyllostachys edulis, PB). The functional traits were analyzed through non-targeted metabolomics based on GC-MS. The results indicated that the PB intercropping system significantly decreased the yield of Bletilla pseudobulb while significantly increasing the total phenol and flavonoids compared with the control (CK). However, there were no significant differences in all economic traits between CB and CK. The functional traits among CB, PB, and CK were separated and exhibited significant differences. Under different intercropping systems, B. striata may adopt different functional strategies in response to interspecific competition. The functional node metabolites (D-galactose, cellobiose, raffinose, D-fructose, maltose, and D-ribose) were up-regulated in CB, while the functional node metabolites (L-valine, L-leucine, L-isoleucine, methionine, L-lysine, serine, D-glucose, cellobiose, trehalose, maltose, D-ribose, palatinose, raffinose, xylobiose, L-rhamnose, melezitose, and maltotriose) were up-regulated in PB. The correlation between economic and functional traits depends on the degree of environmental stress. Artificial neural network models (ANNs) accurately predicted the variation in economic traits via the combination of functional node metabolites in PB. The correlation analysis of environmental factors indicated that Ns (including TN, NH4+-, and NO3--), SRI (solar radiation intensity), and SOC were the main factors that affected the economic traits (yield, total phenol, and total flavonoids). TN, SRI, and SOC were the main factors affecting the functional traits of the Bletilla pseudobulb. These findings strengthen our understanding of the variation of economic and functional traits of Bletilla pseudobulb under intercropping and clarify the main limiting environmental factors under B. striata intercropping systems.
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