REJUVENATION RESEARCH
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2020
·Vol. 23
·期 5
·367-376
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IF 0
Mei Wang,Shan-Quan Chang,Yu-Shan Tian,Ge-Qiang Zhang,Jin Qi
The incidence of type 2 diabetes mellitus (T2DM) has been increasing in recent years and has become a serious threat to human health. Zengye Decoction (ZYD), a well-known traditional Chinese medicinal formula, has been used in the treatment of T2DM with yin asthenia and extreme heat since Qing Dynasty. However, the characteristics of antidiabetic activities of ZYD have not been fully elucidated. In our study, high-fat diet and streptozotocin were used to establish the T2DM rat model. After 3 weeks of treatment with ZYD, the fasting blood glucose (FBG), oral glucose tolerance, the fasting serum insulin concentration, insulin sensitivity index (ISI), serum lipid profiles, and pancreas histopathology were measured. Then, under circumstance of insulin-resistant glucose consumption, 2-(N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) uptake and glycogen content in C2C12 myotubes, 3T3-L1 adipocytes, and HepG2 cells were determined, respectively. Finally, the expressions of key targets in the insulin signaling pathway were measured to explain the potential mechanism underlying these activities. After administration with ZYD, a notable reduction in FBG levels, oral glucose tolerance test-area under the curve, blood lipid metabolism, and ISI values were observed compared with the diabetic control group. Moreover, ZYD restored the damaged islet cells in T2DM rats. Significant increases in glucose consumption, glucose uptake, glycogen content, expression of glucose transporter type 4, and the ratio of p-Akt/Akt were observed in the ZYD groups. According to the above results, ZYD exhibited glucose disposal, including glucose consumption, glucose uptake, and glycogen content and promoted the Akt signal pathway, which indicates that ZYD exerts significant hypoglycemic effect in T2DM.
研究领域:
药理学
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内分泌学
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中医
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2023
·Vol. 31
·期 6
·2653-2667
·
IF 5.3
Naureen Bushra,Ang Bee Chin,Muhamad Farina,Haseeb A. S. M. A.,Basirun W. J.
Polyurethane (PU) are versatile synthetic polymers used for scaffold synthesis. However, their biocompatibility and growth factor (GF) release properties for biomedical and tissue engineering (TE) applications need improvement. We report four kappa-carrageenan (κ-CRG) modified PU (CMPU: PU4C1, PU4C2, PU4C3, and PU4C4) scaffolds from PU4 where κ-CRG % is minimum in PU4C1 and it is maximum in PU4C4. The investigations include fourier transformed infrared (FTIR) spectroscopy, energy-dispersive X-ray (EDX) spectroscopy, in-vitro cell-viability assays using C2C12 myoblasts, and GF such as insulin-like growth factor-1 (IGF-1) release studies using enzyme linked immuno sorbent assay (ELISA) kits. FTIR spectra confirmed the synthesis of CMPU (C = C, COOR, NHCOO, O = S = O, C–O–C, O–SO 3 − ) scaffolds. The EDX spectra presented an increase of sulfur (%) from 0.24% (PU4C1) to 4.0% (PU4C4) with the increase of κ-CRG. The results of biocompatibility assessment day-21 (%) cell viability (PU4 = 67.54 ± 7.68%, PU4C1 = 71.40 ± 5.07%, PU4C2 = 75.79 ± 6.10%, PU4C3 = 82.42 ± 6.82%, PU4C4 = 83.37 ± 7.68%), live/dead images and scanning electron microscope (SEM) images (day 1, 7, and 14) suggest that C2C12 myoblasts adhesion, proliferation, metabolic activity, and viability followed a trend (PU4C4 > PU4C3 > PU4C2 > PU4C1 > PU4). A similar trend was observed in the IGF-1 (ng ml −1 ) release from PU4 and CMPUs (day 1, 3, 7, 10, and 14), suggesting a successful synthesis of CMPUs with enhanced biocompatibility and GF (IGF-1) release, i.e., directly related to the incorporated κ-CRG concentration. Graphical Abstract
研究领域:
生物材料
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组织工程
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Zhang Yunxia,Wu Xiaoxiao,Li Ruoqi,Sui Mengru,Li Guoyin,Fan Shuhua,Yang Mingsheng,Liu Qiuping,Liu Xiaomeng,Wu Changjing,Li Lili
Sodium Danshensu (SDSS) is extracted from Salvia miltiorrhiza and has many pharmacological effects. However, little is known about its effects on muscle fiber formation and metabolism. Here, we aimed to investigated the role and molecular mechanisms of SDSS in modulating the formation of skeletal muscle fiber. C2C12 cells were incubated in differentiation medium with or without SDSS for 4 days. C57BL/6 mice were orally administered SDSS by gavage once a day for 8 weeks. Grip strength, treadmill, muscle weight, western blotting, qPCR, immunofluorescence staining and H&E staining were performed. SDSS target proteins were searched through drug affinity responsive target stability (DARTS) and mass spectrometry analysis. Furthermore, molecular docking was carried out for Pyruvate kinase M1 (PKM1). The effect of PKM1 on myosin heavy chain (MyHCs) gene expression was verified by knockdown of PKM1 experiment. SDSS induced oxidative muscle fiber-related gene expression, and inhibited glycolytic fiber-related gene expression in C2C12 cells. Muscle mass, the percentage of slow oxidative fibers, succinic dehydrogenase activity, muscle endurance, glucose tolerance, and the expression of the MyHC1 and MyHC2a genes increased while MyHC2b expression, lactate dehydrogenase activity, and the percentage of glycolytic muscle fibers decreased in SDSS-treated mice. Mechanistically, SDSS bound to the pyruvate kinase PKM1 and significantly repressed its activity. PKM1 inhibited MyHC1 and MyHC2a expression but promoted MyHC2b expression. SDSS also significantly attenuated the effects of PKM1 on muscle fiber-related gene expression in C2C12 cells. Our findings indicate that SDSS promotes muscle fiber transformation from the glycolytic type to the oxidative type by inhibiting PKM1 activity, which provide a new idea for treating muscle atrophy, muscle metabolism diseases and improving animal meat production.
研究领域:
药理学
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肌肉生物学
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代谢疾病
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分子生物学
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细胞生物学
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动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Lukuan Li,Zhe Wang,Tong Wang,Nannan Zhou,Fang Qiao,Zhen-Yu Du,Mei-Ling Zhang
Short-chain fatty acids (SCFAs) can enhance the growth of aquatic animals and improve flesh quality through the gut-muscle axis. However, the effects of valeric acid, one of the SCFAs, on aquatic animals remain unreported. This study sought to explore the effects of valeric acid on Nile tilapia ( Oreochromis niloticus ). A total of 360 Juvenile tilapia (mean weight, 1.820 ± 0.007 g) with 3 tanks for each treatment were randomly assigned to 12 rectangular acrylic tanks and fed with four isolipidic (6 % lipid) and isonitrogenous (37 % protein) diets for 60 days, including a control diet (CK) and three experimental diets supplemented with 500 mg/kg (CK + LV), 1000 mg/kg (CK + MV), and 2000 mg/kg (CK + HV) of sodium valerate. The results discovered that the enhanced flesh hardness ( p < 0.01) of tilapia caused by valerate was attributed to decreased myofiber size ( p < 0.001). Transcriptome analysis revealed significant enrichment of the Forkhead box O (FOXO) signaling pathway in the valerate-treated group ( p < 0.001). Subsequent qPCR, western blot, immunohistochemistry, and immunofluorescence analyses demonstrated that valerate inhibited muscle cell proliferation by activating the FOXO signaling pathway, suppressing FOXO nuclear export, and consequently downregulating cyclins and cyclin-dependent kinases (CDKs) ( p < 0.01). Furthermore, valerate suppressed muscle differentiation by inhibiting the transcription and protein levels expression of myogenic regulatory factors (MRFs) ( p < 0.001). Additional validation in C2C12 myoblasts and myotubes confirmed that valerate indeed inhibited myoblasts proliferation and myotubes differentiation ( p < 0.001). These findings indicated that valerate reduced myofiber size by suppressing myoblasts proliferation and myotubes differentiation. This study elucidated the effects and mechanisms of valerate on skeletal muscle and introduced a novel approach to enhance fish flesh hardness.
研究领域:
水产养殖
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营养学
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分子生物学
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动物生理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）