SCIENTIA HORTICULTURAE
·
2025
·
IF 4.2
Xia Yang,Yun Wang,Yan Sun,Jiali Guo,Zhaojun Geng,Bingyao Wei,Peiqin Li,Guanghui Tang
Leaf rust caused by Coleosporium zanthoxyli is a destructive disease that threatens Zanthoxylum bungeanum plantations. To address this, screening for resistant cultivars and exploring their resistance mechanisms are crucial for effective disease management. However, the mechanisms underlying Z. bungeanum 's resistance remain unclear. In this study, we conducted in vivo and in vitro experiments to screen for the resistant cultivar Qiujiao (QJ) and the susceptible cultivar Fengxian Dahongpao (FD). Subsequently, the resistance mechanisms of Z. bungeanum were analyzed by integrating transcriptome and metabolome, the result showed the flavonoid pathway served as the primary metabolic pathway for QJ to confer resistance against C. zanthoxyli . Upon infection with C. zanthoxyli , the key genes ( PAL, CHI , and HCT ) and enzymes (PAL, C4H, and 4CL) involved in the flavonoid pathway were upregulated in QJ, and the total flavonoid content was increased in QJ. Additionally, eight flavonoids from this pathway demonstrated inhibitory effects on C. zanthoxyli spore germination, with naringenin and phloretin exhibiting the stronger activity. Collectively, the flavonoid pathway plays a dominant role in the resistance of Z. bungeanum leaves against C. zanthoxyli .
研究领域:
植物病理学
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分子生物学
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转录组学
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代谢组学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2026
·Vol. 2026
·期 1
·1491407
·
IF 2.3
Hui Sun,Sam Al-Dalali,Fei Pan,Long Huang,Min Wei,Jinghan Wang,Rui Dai,Lingyun Wen,Miying Du,Zhigui He
This study investigated the effect of a polyphenolic ethanolic extract from Camellia nitidissima Chi (CNFC) flowers on the reduction of DSS-induced ulcerative colitis in a C57BL/6 mouse model by regulating antioxidant and anti-inflammatory mechanisms. The interaction of the fisetin molecule with essential anti-inflammatory proteins (NF-κB and NLRP3) and the antioxidant protein Keap1 was examined through molecular docking analysis. The phenolic content in CNFC was measured at 2.09 mg/g, while the flavonoid content was 2.80 mg/g. The ethanolic extract of CNFC showed IC 50 values of 0.605, 0.862, and 0.299 mg/mL against DPPH, ABTS, and OH·, respectively, indicating robust antioxidant activity. Three phenolic compounds have been identified for the first time in CNFC flowers: 6-hydroxyflavone, fisetin, and (2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid. The model group mice experienced greater weight loss and had a colon length of 45 mm, much shorter than the intervention group’s 65 mm. The spleen inflammation index was 62.1 mg/10 g, markedly higher than the 44.2 mg/10 g in the intervention group. Additionally, the histopathology score of 7 exceeded that of the intervention group, as did the disease activity index score of 7, compared with the intervention group’s score of 1. The binding interactions of fisetin with NF-κB, Keap1, and NLRP3 proteins indicate a potential affinity for these proteins. CNFC exhibits notable antioxidant and anti-inflammatory properties, and this study offers a technical reference and theoretical foundation for advancing CNFC as a functional and therapeutic food. The present study was designed to elucidate the antioxidant and anti-inflammatory mechanisms of CNFC in DSS-induced colitis, which may serve as a reference for future studies of the multiomics profiles of CNFC’s therapeutic activity, such as gut microbiome profiling, Western blotting, and qPCR-based assessment of related gene expression, for a more detailed understanding.
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产品关联
DOI
高影响因子
Journal of Future Foods
·
2022
·
IF 0
Yue Chen,Yong Zhu,Likang Qin
ABSTRACT In order to study the changes and mechanism of phenolic compounds during Tartary buckwheat germination, the dynamic changes of phenolic compounds were analyzed, and the activities of enzymes that regulate phenolic compound biosynthesis and degradation were monitored. Total phenolics and total flavonoids presented an interesting dynamic trend with the extension of the germination time, and rutin, gallic acid, chlorogenic acid as well as 2,3,4-trihydroxybenzoic acid contents showed an increasing trend during germination period, the contents were up to (2 663.4 ± 61.1), (449.12 ± 5.26), (99.953 ± 7.800) and (50.442 ± 1.477) mg/100 g DW, respectively. Phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) are dominant enzymes in regulating phenolic compound biosynthesis, which showed an increasing trend. Rutin degrading enzyme (RDEs) is the key enzyme in regulating phenolic compound degradation, which showed a decreasing trend. These results suggested that germination processing increases the phenolic compound contents of Tartary buckwheat, which may be regulated by the activation of PAL, CHI and the inhibition of RDEs.
研究领域:
植物科学
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生物化学
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食品科学
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DOI
Current Drug Delivery
·
2023
·Vol. 20
·期 9
·1391-1403
·
IF 2.4
Yu Linjin,Chao Chuyan,Li Qinglan,Ye Songling,Lin Jiasen,Zhong Shuling,Xuan Qiancheng,Xu Kailin,Zhao Suqing
Background: Coenzyme Q10 (Q10) is a powerful lipophilic antioxidant with poor solubility in aqueous media. Curcumin (Cur) is a natural polyphenolic phytochemical molecule with poor aqueous solubility. The liposome is an improved administration of drugs because it is biocompatible and
permeable for nutraceutical delivery. Chitosan, a hydrophilic polymer, is often used as a polymer coating for its good biocompatible and biodegradable properties, and its relatively low toxicity level. Methods: Q10 and Cur co-loaded liposomes coated with chitosan (Q10-Cur-Lip-Chi) were
constructed. The co-encapsulation of Q10 and Cur in liposomes coated with chitosan was verified by TEM, DLS, DSC, FT-IR, and XRPD. The release profile and antioxidant activity of Q10-Cur-Lip-Chi were accessed. Results: The particle size of Q10-Cur-Lip-Chi was about 1440 nm with narrow
particle distribution. A satisfactory encapsulation efficiency (EE) of Q10 was about 98%, and 25% for that of Cur. Q10-Cur- Lip-Chi showed higher solubility and better pH resistance with 98.5% of Q10 and Cur retention at pH 7.0 - 9.0. Q10-Cur-Lip also showed great salt stability with a vesicle
size change of less than 5%. PSof Q10-Cur-Lip-Chi changed less than 10% at 4°C of storage. Q10-Cur-Lip-Chi also exhibited a good controlled release profile with its accumulative release of less than 34% for Q10 and 30% for curcumin after 24 h. The Q10-Cur-Lip-Chi performed a synergistic
effect on antioxidant activity reaching 41.86±1.84%, which was 5.9 times higher than that of Q10, 2.5 times higher than that of Cur, and 1.7 times higher than that of the mixture. Conclusion: The co-encapsulation Q10-Cur-Lip-Chi improves the solubility and stability of Q10 and
Cur for good release performance and antioxidative activity.
研究领域:
药剂学
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纳米技术
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药物递送系统
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生物物理学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2022
·Vol. 33
·期 8
·1-14
·
IF 4.727
Xu Hongjie,Wang Jingjing,Wu Di,Qin Dajiang
Background Diabetic wound is a severe complication of diabetes. Stem cell is considered as a promising therapy for diabetic skin wounds. Hydrogel can supply niche for cells adhesion and survival to improve the efficacy of stem cell therapy, but the development of hydrogel with suitable properties remains a great challenge. Thus, our study was conducted to combine an optimized hydrogel with stem cell to improve complex diabetic wound treatment. Methods This study constructed a hydrogel with low toxicity and adjustable mechanical properties from gelatin methacrylate (GelMA) and chitosan-catechol (Chi-C), and encapsulated human umbilical cord-mesenchymal stem cells (hUMSCs) to repair full-thickness diabetic wound. Results We explored the relationship between mechanical stiffness and cell proliferation and differentiation potency, and found 10% GelMA hydrogel with an optimal stiffness improved hUMSCs adhesion, proliferation, and differentiation potency maintenance in vitro. Assistant with optimized hydrogel encapsulating hUMSCs, diabetic wound healing process was greatly accelerated, including accelerated wound closure, inhibited secretion of inflammatory factors TNF-α and IL-1β, promoted vascular regeneration and collagen deposition after treatment of hUMSCs. Conclusions The optimized hydrogel encapsulating hUMSCs improved diabetic wound healing, and has a broad implication for the treatment of diabetic complication.
Diabetic wound is a severe complication of diabetes. Stem cell is considered as a promising therapy for diabetic skin wounds. Hydrogel can supply niche for cells adhesion and survival to improve the efficacy of stem cell therapy. This study constructed a hydrogel with low toxicity and adjustable mechanical properties from gelatin methacrylate (GelMA) and chitosan-catechol (Chi-C), and encapsulated human umbilical cord-mesenchymal stem cells (hUMSCs) to repair full-thickness diabetic wound. Hydrogel of 10% GelMA with an optimal stiffness improved hUMSCs adhesion, proliferation, and differentiation potency maintenance in vitro. Assistant with optimized hydrogel encapsulating hUMSCs, diabetic wound healing process was greatly accelerated, including accelerated wound closure, inhibited secretion of inflammatory factors TNF-α and IL-1β, promoted vascular regeneration and collagen deposition after treatment of hUMSCs. The study supplies an alternative treatment for diabetic complication. Hydrogel-hUMSCs combined treatment accelerates wound closure in diabetic mice. A . Representative images of wounds during 21-day in vivo experiments. B . Quantification of wound closure rate (%) over 21-day period. C . HE staining of wounds at days 7, 14 and 21. The bar corresponds to 200 μm.
研究领域:
生物医学工程
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组织工程
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干细胞研究
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伤口愈合
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 20
·13958-13971
·
IF 3.9
Bin Wang,Yanbin Zhao,Yangyang Li,Junyan Yao,Shunjie Wu,Guoping Miu,Chenglin Chu
The insufficient osteogenesis of magnesium phosphate cement (MPC) limits its biomedical application. It is of great significance to develop a bioactive MPC with osteogenic performance. In this study, an injectable MPC was reinforced by the incorporation of a near infrared (NIR)-responsive nanocontainer, which was based on simvastatin (SIM)-loaded mesoporous silica nanoparticles (MSNs) modified with a polydopamine (PDA) bilayer, named SMP. In addition, chitosan (CHI) was introduced into MPC (K-struvite) to enhance its mechanical properties and cytocompatibility. The results showed that nanocontainer-incorporated MPC possessed a prolonged setting time, almost neutral pH, excellent injectability, and enhanced compressive strength. Immersion tests indicated that SMP-CHI MPC could suppress rapid degradation. Based on its physicochemical features, the SMP-CHI MPC had good biocompatibility and osteogenesis properties, as shown via in vitro and in vivo experiments. These findings can provide a simple way to produce a multifunctional MPC with improved osteogenesis for further orthopedic applications.
研究领域:
材料科学
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生物医学工程
·
纳米技术
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Wenhao Wang,Ao Wang,Gaofu Hu,Mengyao Bian,Lili Chen,Qian Zhao,Weilian Sun,Yanmin Wu
Periodontal tissue regeneration is a major challenge in tissue engineering due to its regenerated environment complexity. It aims to regenerate not only the supporting alveolar bone and cementum around teeth but also the key connecting periodontal ligament. Herein, a constructed aligned porous hydrogel scaffold carrying cells based on chitosan (CHI) and oxidized chondroitin sulfate (OCS) treated with a freeze-casting technique was fabricated, which aimed to induce the arrangement of periodontal tissue regeneration. The microscopic morphology and physical and chemical properties of the hydrogel scaffold were evaluated. The biocompatibilities with periodontal ligament stem cells (PDLSCs) or gingival-derived mesenchymal stem cells (GMSCs) were verified, respectively, by Live/Dead staining and CCK8 in vitro. Furthermore, the regeneration effect of the aligned porous hydrogel scaffold combined with PDLSCs and GMSCs was evaluated in vivo. The biocompatibility experiments showed no statistical significance between the hydrogel culture group and blank control (P > 0.05). In a rat periodontal defect model, PDLSC and GMSC hydrogel experimental groups showed more pronounced bone tissue repair than the blank control (P < 0.05) in micro-CT. In addition, there was more tissue repair (P < 0.05) of PDLSC and GMSC hydrogel groups from histological staining images. Higher expressions of OPN, Runx-2, and COL-I were detected in both of the above groups via immunohistochemistry staining. More importantly, the group with the aligned porous hydrogel induced more order periodontal ligament formation than that with the ordinary hydrogel in Masson’s trichrome analysis. Collectively, it is expected to promote periodontal tissue regeneration utilizing an aligned porous hydrogel scaffold combined with PDLSCs and GMSCs (CHI–OCS–PDLSC/GMSC composite), which provides an alternative possibility for clinical application.
研究领域:
组织工程
·
牙科材料
·
干细胞生物学
·
再生医学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）