BioChip Journal
·
2021
·Vol. 15
·期 3
·268-275
·
IF 3.494
Gao Nailong,Chang Jianguo,Zhu Ziming,You Hui
Sufficiently long reaction time and low flow velocity are crucial for capillary microfluidic immunoassay chips, especially for truly “one-step” detection with continuous on-chip labelling and capturing. In this study, a multistory stairs-based new delay valve is used to meet the time and flow requirements for protein testing. The delay strategy is capable of slowing down the flow of liquid into the functional region and delaying the time out of the regions as well, thus extending the labelling and capture time to about 100 and 60 s respectively, and further reducing the flow velocity to below 8 μL/min. Based on the principle of sandwich-like immunocomplex, the designed chip is capable of automatically accomplishing on-chip mixing, labelling and capturing procedures via once sample loading and sensitively detecting human Alpha-Fetoprotein (AFP) in 40 μL serum with a wide dynamic ranging from 10 ng/mL to 10 000 ng/mL within 5 min. It could be foreseeable that this microchip has great potential in biomarkers clinical testing.
研究领域:
生物医学工程
·
微流控技术
·
即时诊断
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生物标志物检测
·
免疫分析
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DOI
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
·
2022
·Vol. 59
·期 1
·1-9
·
IF 2.723
Chen Sunyu,Xiao Zhanhao,Jiang Wenjin
Osteogenic differentiation is a crucial process of new bone formation. This study aimed to explore the roles and mechanism of SRY-Box Transcription Factor 2 (SOX2) on proliferation and osteogenic differentiation of MC3T3-E1 cells. Bone morphogenetic protein 2 (BMP2) was used to induce the osteogenic differentiation of MC3T3-E1 cells. The expression of SOX2 was determined by quantitative real-time PCR (RT-PCR) at different time points after induction. The SOX2 overexpression plasmids were constructed and transfected into MC3T3-E1 cells. Osteogenic differentiation was evaluated by Alizarin Red S staining and alkaline phosphatase (ALP) assay. The expressions of osteogenic differentiation markers including runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) were detected by western blot assay. Luciferase reporter and CHIP assays were used to confirm that SOX2 regulated the transcriptional activation of leucine-rich repeat-containing G protein–coupled receptor 4 (LGR4). We found that SOX2 was down-regulated upon BMP2-induced osteogenic differentiation in MC3T3-E1 cells. Overexpression of SOX2 effectively inhibited osteogenic differentiation with decreased ALP activity, calcification, and osteogenic differentiation markers’ expression including Runx2, OPN, and OCN. LGR4 was identified as a target of SOX2, and the inhibitory effect of SOX2 on osteogenic differentiation was reversed by knockdown of LGR4. The present study confirmed that SOX2 suppressed osteogenic differentiation of MC3T3-E1 cells through targeting LGR4, which possesses a therapeutic strategy for bone formation and generation.
研究领域:
分子生物学
·
细胞生物学
·
骨骼生物学
·
转录调控
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产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
·
2026
·
IF 6.3
Xian Chen,Chuanchao Zhang,Yaping Wang,Xuexin Duan,Yunhua Gao
Micro-mixing plays an important role in the biochemical analyses using miniaturized total analysis system (µTAS) or lab-on-chips. However, the existing micromixers primarily rely on the specific medium and lack the capability of being activated on-demand, and it is rather challenging to ensure the adequate analyte capture and fluid washing on the surface of each probe particle during the assay steps. Herein, we demonstrated a novel acoustic chaotic micromixer actuated by a Lamb wave resonator (LWR) array, which integrated all steps from the initial sample pretreatment, chaotic probe particle mixing, analytes trapping, fluid washing to the ultimate signal acquisition, thereby fulfilling the “sample-in-answer-out” medical needs. The high-speed microscale acoustic streaming vortices triggered by the four LWRs in the confined micro-channel can be utilized to accelerate the particles’ velocity and enhance the collision between probe particle and target analyte. In addition, chaotic particle mixing effect was realized by on-demand controlling the direction and magnitude of the four-in-one drag force based on the programmable acoustic stimuli of each independent LWR. Besides, a compact cartridge was specifically designed to accommodate the acoustic chaotic micromixer and peripheral accessories (including RF signal source, CMOS optical sensor and mini pump), thus presenting a handheld device for point-of-care applications. Finally, the trace prostate specific antigen (PSA) derived from fingertip blood samples was effectively detected. Owning to the compact size, on-chip chaotic particle mixing, fast and sensitive detection performance, the proposed acoustofluidic system is very suitable for the on-site diagnostic applications.
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Xinlian Zhang,Xi Chen,Yuhan Yao,Xiaona Shang,Huijun Lu,Wang Zhao,Sixiu Liu,Jianmin Chen,Guodong Sui
Acute toxicity assay presents vital significance in modern environmental monitoring, including online detection and in-situ assay for emergency events. Although photobacteria related detection methods were established and verified in the past decades with combination of photomultiplier tube (PMT), the price and size of PMT sensor hampered application of rapid acute toxicity assay and detection system miniaturization, especially in the resource-limited occasions. Wide application of smartphones with great low-light performance cameras could be used in photobacteria-based toxicity assay instead of the PMT methods. Herein a box-type portable detection system had been successfully established, including a disc-chip for detection, detection device, and smartphones with a high-performance camera. The system performed well showing stable temperature and rotation control. Results captured by CMOS-based camera presented a linear relationship with PMT-based detection method. An image progress algorithm was also established and tested by series diluted zinc sulfate solution as a reference substance. The system also performed well for toxicity analysis for real Atmospheric particle matter sample. The system could be used in some environmental monitoring scenarios as an alternative solution.
研究领域:
环境科学
·
毒理学
·
生物传感器
·
微流控技术
·
即时检测
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MODERN PHYSICS B
·
2019
·
IF 0.863
Jiaqi Wang,Chao Bian,Yang Li,Jizhou Sun,Jianhua Tong,Shanhong Xia
Water pollution has always been an important issue threatening human health. Water quality monitoring is an important step to ensure water quality safety. Water quality parameters such as pH, temperature and conductivity are important indicators in water quality monitoring. In this paper, a multi-parameter water quality detection integrated chip system is studied, and multiple sensors are integrated on the same chip to realize the monitoring of conventional parameters such as pH, temperature and conductivity in drinking water. The chip system was processed by MEMS technology. A ruthenium oxide (RuO 2 ) film is used for pH detection, and the sensor exhibits a super-Nernst response (62.88 mV/pH) in the range of pH 1.92–11.74. The patterned micro Pt film is used as the temperature sensor, and the three-wire orthogonal structure is used for temperature detection. The sensitivity of this temperature sensor is 5.517 Ω / ∘ C in the range of 6 to 80 ∘ C. Conductivity was measured by a four-electrode system, and the electrode constant was 1.667 cm − 1 measured between 0.994 and 21.167 mS/cm. The test results show that the multi-parameter integrated chip system can realize the simultaneous detection of pH, temperature and conductivity in water, and has the characteristics of miniaturization and low cost, and provides an effective way for simultaneous detection of water quality.
研究领域:
环境监测
·
微机电系统（MEMS）
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传感器与仪器仪表
·
水质分析
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DOI
高影响因子
iScience
·
2025
·
IF 4.1
Hongwei Su,Qing Guo,Hongtao Li,Alei Li,Yunlei Zhong,Xu Zhang,Lin Geng,Shuai Liu,Liuqi Dong,Xiaohang Pan,Lin Wang,Lixing Kang
Developing low-cost, high-performance metal-free electrocatalysts is crucial for sustainable oxygen evolution reactions (OERs) in alkaline media. Here, we synthesized ultra-long boron phosphide (BP) microwires along the [111] crystal axis with high yield. By controlling temperature and adding Ni, we identified Ni’s role as a flux and transport agent, optimizing BP microwires at 1050°C. Electrical property and band structure analysis revealed BP as a one-dimensional p-type semiconductor with a wide band gap. We constructed an on-chip electrocatalytic microdevice using individual BP microwires to evaluate OER performance. In 1M NaOH, the BP electrode achieved 50 mA cm −2 at an overpotential of 320 mV, outperforming other boron-based catalysts. Additionally, the nanocatalyst exhibited a low Tafel value (88 mV·dec −1 ) and excellent stability. This study offers valuable insights for developing future electrocatalysts and electrochemical reaction models.
研究领域:
材料科学
·
电化学
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DOI
PubMed
高影响因子
Small
·
2015
·Vol. 11
·期 23
·2798-2806
·
IF 8.368
Lei Wu,Zhuyuan Wang,Kequan Fan,Shenfei Zong,Yiping Cui
A surface enhanced Raman scattering (SERS)-assisted 3D barcode chip has been developed for high-throughput biosensing. The 3D barcode is realized through joint 2D spatial encoding with the Raman spectroscopic encoding, which stores the SERS fingerprint information in the format of a 2D array. Here, the concept of SERS-assisted 3D barcode is demonstrated through multiplex immunoassay, where simultaneous detection of multiple targets in different samples has been achieved using a microfluidic platform. First, multiple proteins in different samples are spatially separated using a microfluidic patterned antibody barcode substrate, forming a 2D hybridization array. Then the SERS probes are used to identify and quantify the proteins. As different SERS probes are labeled with different Raman reporters, they could be employed as “SERS tags” to incorporate spectroscopic information into the 3D barcode. In this 3D barcode, the 2D spatial information helps to differentiate the samples and targets while the SERS information allows quantitative multiplex detection. It is found that the SERS-assisted 3D barcode chip can not only accomplish one-step multiplex detection within 30 min but also achieve an ultrasensitivity down to 10 fg mL −1 (≈70 aM), which is expected to provide a promising tool for high-throughput biomedical applications.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL MICRODEVICES
·
2019
·Vol. 21
·期 1
·1-13
·
IF 2.327
Shen Yiren,Du Kun,Zou Lili,Zhou Xiaoming,Lv Rong,Gao Dayong,Qiu Bensheng,Ding Weiping
Freeze-drying is a promising approach for the long-term storage of erythrocytes at room temperature. Studies have shown that trehalose loaded into erythrocytes plays an important role in protecting erythrocytes against freeze-drying damage. Due to the impermeability of the erythrocyte membrane to trehalose, many methods have been developed to load trehalose into erythrocytes. However, these methods usually require multistep manual manipulation and long processing time; the adopted protocols are also diverse and not standardized. Thus, we develop an osmotically-based trehalose-loading microdevice (TLM) to rapidly, continuously, and automatically produce erythrocytes with loaded trehalose. In the TLM, trehalose is loaded through the erythrocyte membrane pores induced by hypotonic shock; then, the trehalose-loaded erythrocytes are rinsed to remove hemoglobin molecules and cell fragments, and the extracellular solution is restored to the isotonic state by integrating a rinsing-recovering design. First, the mixing function and the rinsing-recovering function were confirmed using a fluorescent solution. Then, the performance of the TLM was evaluated under various operating conditions with respect to the loading efficiency of trehalose, the hemolysis rate of erythrocytes ( ϕ ), the recovery rate of hemoglobin in erythrocytes ( φ ), and the separation efficiency of the TLM. Finally, the preliminary study of the freeze-drying of erythrocytes with loaded trehalose was accomplished using the TLM. The results showed that under the designated operating conditions, the loading efficiency for human erythrocytes reached ~21 mM in ~2 min with a ϕ value of ~17% and a φ value of ~74%. This study provides insights into the design of the on-chip loading of trehalose into erythrocytes and promotes the automation of life science studies on biochips.
研究领域:
生物技术
·
细胞生物学
·
微流控
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Long Li,Jibran Iqbal,Hui Li,Yuan Zhang,Lin Sun,Ying Zhu,Wanchao Chen,Yongqing Xi,Ting Wu,Yiping Du
A device was designed integrating solid phase extraction (SPE) and in situ optical detection techniques. A chip assembled in the device to carry out SPE was enclosed in a fully reflective aluminum box to avoid loss of radiation flux in the optical measurement. The spectrum was measured on the SPE material directly after SPE. The device offers simple analytical operation without any elution step comparing with the traditional analytical methods with complicated procedures. The device and the developed analytical technique was used for the determination of rhodamine B and benzo[ a ]pyrene with UV–visible diffuse reflectance and solid phase fluorescence spectroscopies, respectively. Under optimal conditions, standard curves of rhodamine B and benzo[ a ]pyrene showed a linear relationship in concentration ranges of 10–800 μg L -1 (R 2 = 0.99) and 0.07–3.33 μg L -1 (R 2 = 0.99), respectively. The limit of detections (LOD) were 7 μg L -1 and 0.02 μg L -1 . The proposed device was successfully employed to quantify rhodamine B and benzo[ a ]pyrene in tap water samples. The results showed that the recoveries were 90–105% and the relative standard deviations (RSDs) were 2.53–5.55%, indicating that the device and analytical method is capable of trace analytes for rapid quantitative analysis in various samples.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Xiaolu Xing,Ying Zhang,Yan Tan,Tianhao Hu,Ziqiu Chen,Bowen Liu
Surface enhanced Raman spectroscopy (SERS) offers ultrasensitive and label-free detection of specific molecules with broad applications in chemistry, life sciences, security, environmental sciences, and sensors. Among environmental contaminants, Hg2+ poses a significant threat due to its high toxicity, necessitating precise and high-throughput detection methods. Here, we introduce a novel SERS chip that integrates a multiwell plate with a plasmonic array, boasting strong electromagnetic enhancement and exceptional uniformity. Through surface modification of the chip with I–, we achieve high throughput, selectivity, and low limit of detection (LOD) for Raman-inactive Hg2+. Notably, the chip serves as a convenient platform for real-time Hg2+ detection and offers versatility for various surface modifications, paving the way for a next-generation quantitative and high-throughput sensor for a Raman-inactive analyte.
研究领域:
环境科学
·
分析化学
·
生物传感
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产品关联
DOI
高影响因子
LAB ON A CHIP
·
2022
·Vol. 23
·期 2
·341-348
·
IF 7.517
Haiying Shen,Qiliang Li,Wenqi Song,Xingyu Jiang
On-chip valves can simplify a microfluidic chip and make it easy to operate. However, most on-chip valves already reported still need complicated manufacture and sophisticated supporting devices. In this work, we present a straightforward on-chip valve, which can be serially connected, to form an on-chip pump. The liquid can horizontally flow one way by the regular deformations of flexure strips in the two valves at both sides of the chamber under pressure changes in microchannels generated by repeated vertical movements of linear actuators. The volume of this system including the chip and the supporting device is 0.65 cubic decimeters, which is much smaller than that of reported systems with a volume of at least 12 cubic decimeters, and the weight of this system is only 0.56 kg, making it possible for point-of-care testing. We carry out an immunoassay of folic acid on chip, and the results show satisfactory reproducibility with acceptable coefficients of variation. We determine 163 clinical human serum samples for folic acid. Furthermore, we detect transferrin, cobalamin and folic acid simultaneously on one chip with both sandwich and competitive binding immunoassay methods. We anticipate that this on-chip valve and pump can be applied in immunoassays and other biosensing applications.
研究领域:
微流控
·
生物传感
·
即时诊断
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Biomedical Optics Express
·
2023
·Vol. 14
·期 6
·3003-3017
·
IF 3.4
Tingting Yu,Tingting Yu,Xiang Zhong,Xiang Zhong,Qihang Yang,Qihang Yang,Chao Gao,Chao Gao,Wenyue Chen,Wenyue Chen,Xiang Liu,Xiang Liu,Zhang Liu,Zhang Liu,Tingting Zhu,Tingting Zhu,Dongyu Li,Dongyu Li,Peng Fei,Peng Fei,Zaozao Chen,Zaozao Chen,Zhongze Gu,Zhongze Gu,Dan Zhu,Dan Zhu
Three-dimensional (3D) cell cultures provide an important model for various biological studies by bridging the gap between two-dimensional (2D) cell cultures and animal tissues. Microfluidics has recently provided controllable platforms for handling and analyzing 3D cell cultures. However, on-chip imaging of 3D cell cultures within microfluidic devices is hindered by the inherent high scattering of 3D tissues. Tissue optical clearing techniques have been used to address this concern but remain limited to fixed samples. As such, there is still a need for an on-chip clearing method for imaging live 3D cell cultures. Here, to achieve on-chip clearing for live imaging of 3D cell cultures, we conceived a simple microfluidic device by integrating a U-shaped concave for culture, parallel channels with micropillars, and differentiated surface treatment to enable on-chip 3D cell culture, clearing, and live imaging with minimal disturbance. The on-chip tissue clearing increased the imaging performance of live 3D spheroids with no influence on cell viability or spheroid proliferation and demonstrated robust compatibility with several commonly used cell probes. It allowed dynamic tracking of lysosomes in live tumor spheroids and enabled quantitative analysis of their motility in the deeper layer. Our proposed method of on-chip clearing for live imaging of 3D cell cultures provides an alternative for dynamic monitoring of deep tissue on a microfluidic device and has the potential to be used in 3D culture-based assays for high-throughput applications.
研究领域:
生物医学工程
·
细胞生物学
·
显微成像技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）