Coatings
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2018
·Vol. 8
·期 10
·372
·
IF 2.35
Yang Gao,Chaonan Kan,Ming Chen,Chuying Chen,Yuhuan Chen,Yongqi Fu,Chunpeng Wan,Jinyin Chen
The current work aimed to evaluate the efficacy of four coating formulations—chitosan coating (CH), 0.5% cinnamaldehyde + chitosan (0.5% CI–CH), 1.0% cinnamaldehyde + chitosan (1.0% CI–CH), and 1.5% cinnamaldehyde + chitosan (1.5% CI–CH)—on fresh mandarin fruit cv. Ponkan quality maintenance (weight loss, decay rate, total soluble solids, titratable acidity, vitamin C, color index, malondialdehyde, and antioxidant activity) over 100 days of storage at 20 °C. Compared to the control, chitosan treatment effectively reduced the decay and weight loss rates of mandarin fruit cv. Ponkan during storage at room temperature, delayed the decline of nutritional quality in fruits, increased the antioxidant capacity, and inhibited the accumulation of malondialdehyde (MDA). In comparison to chitosan coating, 1.5% CI–CH did not improve the fruit storage effect, but inhibited the normal color change of fruits and increased the accumulation of MDA. Both 0.5% CI–CH and 1.0% CI–CH effectively reduced the rate of fruit decay, improved the quality of fruits after harvest, and delayed fruit aging. Our study suggests that 0.5% CI–CH and 1.0% CI–CH might be good formulations for maintaining the quality of mandarin fruit cv. Ponkan during room-temperature storage.Keywords:chitosan;coating;cinnamaldehyde;fruit;room temperature
研究领域:
食品科学
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植物保护
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 25
·期 1
·12
·
IF 3.06
Mingyi Yang,Lei Wang,Tarun Belwal,Xiaocheng Zhang,Hongyan Lu,Cunkun Chen,Li Li
Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis viniferacv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.Keywords:grape;melatonin;abscisic acid;flavonoid;Vitis vinifera
研究领域:
植物生理学
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分子生物学
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农业科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Ziheng Yang,Li Jiang,Weidan Zhao,Boya Shi,Xiangwen Qu,Yekai Zheng,Pengwei Zhou
Recent years, two-dimensional transition metal carbonitrides (MXene) have attracted much attention in the field of surface-enhanced Raman scattering (SERS). However, the relatively low enhancement of MXene is a major challenge. Herein, Nb 2 C-Au NPs nanocomposites were prepared by electrostatic self-assembly method, which have a synergistically conjugated SERS effect. The EM hot spots of Nb 2 C-Au NPs are significantly enlarged and expanded, while the surface Fermi level is decreased. This synergistic effect could improve the SERS performance of the system. Consequently, for the dye molecules CV and MeB, the detection limits reach 10 −10 M and 10 −9 M, respectively, while for biomolecule adenine, the detection limit is as low as 5 × 10 −8 M. The results also show the good concentration-dependent linearity, uniformity, reproducibility and stability of SERS substrate. Nb 2 C-Au NPs could be a fast, sensitive and stable SERS platform for label-free and non-destructive detection. This work may expand the application of MXene based materials in the field of SERS.
研究领域:
材料科学
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纳米技术
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分析化学
·
光学
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PubMed
高影响因子
Journal of Future Foods
·
2026
·
IF 7.2
Rui Wang,Pei-Xing Li,Cui-Wen Deng,Yi Chen,Yuan-Yi Mo,Yu-Wei Wu,Qi-Qing Liang,Ai Xu,Ben Yu,Lang-Hong Wang,Xin-An Zeng
Loquat fruit is highly susceptible to chilling injury during low-temperature storage, leading to tissue browning and quality deterioration, which severely limits its postharvest commercial value. This study investigated the efficacy of pulsed electric field (PEF) pretreatment in maintaining the quality of cold-stored loquat fruit at 1°C. The results showed that PEF pretreatment exerted dose-dependent effects on fruit quality preservation. Treatment at 0.3 kV/cm effectively suppressed browning and reduced the decay rate to 23.20 ± 2.44% after 16 days of storage, significantly lower than that of the control (46.93 ± 1.14%). Additionally, this treatment better maintained fruit firmness, minimized weight loss, and preserved higher vitamin C content, thereby improving both visual and nutritional quality. However, quality declined at a higher PEF intensity of 0.5 kV/cm, likely due to excessive electroporation-induced cellular damage. During early storage, the 0.3 kV/cm treatment increased total phenolic content by 72.36% and sustained higher total flavonoid levels (0.84–0.99 mg/g) throughout storage. Concurrently, polyphenol oxidase (PPO) activity decreased to 33.94 ± 0.66 U/g by day 16, while peroxidase (POD) activity remained consistently lower than in the control. The elevated accumulation of antioxidant compounds, coupled with the inhibition of key browning-related enzymes, appears to be the primary mechanism by which PEF alleviates chilling-induced browning. In conclusion, PEF pretreatment offers an effective strategy for extending the shelf life of cold-stored loquat fruit and shows potential for application in other chilling-sensitive fruits.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Fu Xiang,Jinhai Bai,Xiaobei Tan,Ting Chen,Wei Yang,Feng He
Essential oil is one of the most important bioactive substances of Artemisia argyi Levl. et Van. var. argyi cv. Qiai, a traditional Chinese herb used in folks for centuries. An essential oil was prepared by steam distillation method. Chemical components of the essential oil were explored by gas chromatography-mass spectrometry. Antimicrobial activities of the essential oil were determined by agar disc diffusion assay and double-broth dilution method. Also, antimicrobial mechanisms were investigated based on cytomembrane permeability, protein and K + leakage and electron microscopy. The results showed that the essential oil yield was (0.7 ± 0.02)% (w/w). A total of 32 components were identified, and thujone was found to be the major compound (14.94%), followed by globulol (11.6%), 1,8-cineole (11.31%), 1-caryophyllene (8.11%), camphor (6.87%), α-terpineol (4.69%), caryophyllene oxide (4.05%), borneol (3.95%), eugenol (3.01%), and germacrene D (2.81%). The oil showed antimicrobial activities against both Gram-positive and Gram-negative bacteria. The minimum inhibition concentrations of the essential oil against Staphylococcus aureus , Bacillus cereus , Bacillus subtilis , Listeria monocytogenes , Escherichia coli , Pseudomonas aeruginosa , and Salmonella Typhimurium were 16.0, 16.0, 32.0, 32.0, 32.0, 64.0, and 32.0 μg/mL, respectively. The minimum bactericidal concentrations of the essential oil against bacteria mentioned above were 32.0, 16.0, 64.0, 64.0, 64.0, 128.0, and 64.0 μg/mL, respectively. Supposedly, the possible antimicrobial mechanism of the essential oil involved simultaneous cytomembrane disruption, leading to the leakage of intracellular substances, such as protein and K + . The results indicated that the cytomembrane might be the target of essential oil.
研究领域:
药理学
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微生物学
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化学
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植物学
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Xiaopeng Lu,Hua Weng,Chao Li,Jun He,Xing Zhang,Zhiqing Ma
Mosla chinensis Maxim. cv. Jiangxiangru (MCJ) is a traditional Chinese herb, which can be obtained in large quantities by artificial cultivation. In order to understand industrial value of MCJ resources, the essential oil (EO) of MCJ was obtained by steam distillation, analyzed by gas chromatography-mass spectrometry (GC-MS), and evaluated by bioassay and greenhouse trials against Mythimna separate , Myzus persicae , Sitophilus zeamais , Musca domestica and Tetranychus cinnabarinus . The GC-MS results showed that the main components in MCJ EO were thymol (50.60 %), β -thymol (22.32 %) and carvacrol (18.44 %), accounting together for more than 90 % of all compositions. MCJ EO and its three main components showed high fumigant activity against five species insect pests, in the descending order (LC 50 , μL/L air): T. cinnabarinus adults (3.83), M. persicae adults (4.34), M. separate 3rd instar larvae (4.49), M. domestica adults (5.66), and S. zeamais adults (53.71). Moreover, thymol exhibited higher fumigation toxicity than β -thymol and carvacrol, with the LD 50 values of 1.66–59.20 μL/L air to five species insect pests. Greenhouse experiments indicated that MCJ EO 10 % emulsion exhibited obvious corrected control efficacy of about 87.92 % and 77.34 % against M. persicae and T. cinnabarinus on kidney bean ( Phaseolus vulgaris ) plants after 7 days treatment at 300 g hm −2 , respectively. As a traditional Chinese medicinal herb with a large-scale planting area, MCJ provides low-cost, abundant raw materials for production of MCJ EO. And MCJ EO, a safe and broad-spectrum insecticidal active ingredient, has the potential to be developed as novel botanical pesticides for the management of agricultural, sanitary and stored pests.
研究领域:
植物学
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昆虫学
·
农业科学
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高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2017
·Vol. 19
·期 33
·22472-22481
·
IF 4.123
Jiying Liang,Xue Yu,Tiangang Yang,Menglu Li,Li Shen,Yue Jin,Hongyun Liu
In this paper, poly(N-isopropylacrylamide-co-3-aminophenylboronic acid) (P(NIPAM-co-APBA)) copolymer films were successfully electropolymerized on the Au electrode surface. The electroactive probe ferrocene carboxylic acid (FCA) in solution showed reversible thermal-, glucose- and pH-responsive on–off cyclic voltammetric (CV) behaviors at the film electrodes. The comparative experiments demonstrated that the thermo-responsive property of the film electrode was ascribed to the PNIPAM component of the films, whereas the glucose- and pH-sensitive behaviors came from the PAPBA constituent. The reduced form of nicotinamide adenine dinucleotide (NADH) could be electrocatalytically oxidized by FCA at the film electrodes, which would greatly amplify the multi-responsive CV signal difference between the on and off states. On the basis of these results, a binary 4-input/4-output logic circuit was fabricated with temperature, glucose, pH and NADH as inputs and the CV responses at 4 different levels as outputs. Moreover, a ternary CONSENSUS logic circuit was established on the same platform, which was the first report on the combination of ternary logic gate and bioelectrocatalysis without using enzymes. This work provided a novel idea for constructing complicated biocomputing systems by increasing the number of inputs/outputs with multi-sensitive interfaces and by designing new types of multi-valued logic gates on the basis of bioelectrocatalysis.
研究领域:
生物计算系统
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生物电化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2024
·Vol. 191
·期 7
·1-13
·
IF 5.3
Wang Yuefan,Chen Yinzhi,Zhou Yuhe,Wang Yilin,Wu Yingjie,Xie Yixi,Zhao Pengcheng,Hu Xiayi,Fei Junjie
Rutin extracted from natural plants has important medical value, so developing accurate and sensitive quantitative detection methods is one of the most important tasks. In this work, HKUST-1@GN/MoO 3 -Ppy NWs were utilized to develop a high-performance rutin electrochemical sensor in virtue of its high conductivity and electrocatalytic activity. The morphology, crystal structure, and chemical element composition of the fabricated sensor composites were characterized by SEM, TEM, XPS, and XRD. Electrochemical techniques including EIS, CV, and DPV were used to investigate the electrocatalytic properties of the prepared materials. The electrochemical test conditions were optimized to achieve efficient detection of rutin. The 2-electron 2-proton mechanism, consisting of several rapid and sequential phases, is postulated to occur during rutin oxidation. The results show that HKUST-1@GN/MoO 3 -Ppy NWs have the characteristics of large specific surface area, excellent conductivity, and outstanding electrocatalytic ability. There is a significant linear relationship between rutin concentration and the oxidation peak current of DPV. The linear range is 0.50–2000 nM, and the limit of detection is 0.27 nM (S/N = 3). In addition, the prepared electrode has been confirmed to be useful for rutin analysis in orange juice. Graphical Abstract
研究领域:
分析化学
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材料科学
·
电化学
·
食品科学
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PubMed
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Fuwei Sun,Tianhu Chen,Ziyang Chu,Peixun Zhai,Haibo Liu,Qiang Wang,Xuehua Zou,Dong Chen
A system of Cu 2+ /calcite/PDS was constructed to degrade sulfadiazine (SDZ). Different from the traditional Cu-mediated activation, a low concentration of Cu 2+ that met drinking water standards (≤ 1 mg/L) transformed into Cu(Ⅱ) solid in the presence of calcite, and then enhanced the degradation of SDZ via PDS activation over a pH range from 3 to 9. According to scavenger and chemical probe experiments, Cu(Ⅲ), rather than radicals (hydroxyl radicals and sulfate radicals) and singlet oxygen, was the predominant reactive species, which was responsible for the degradation of SDZ. Based on the results of XRD, ATR-FTIR, and CV curves et al., CuCO 3 was the main complex with high reactivity for PDS activation to form Cu(Ⅲ). Moreover, detailed degradation pathways of sulfadiazine were proposed according to the UPLC-ESI-MS/MS and their toxicity was predicted by ECOSAR. Besides, the real water matrix would not seriously affect the degradation of SDZ in the Cu 2+ /calcite/PDS system. In summary, this study reveals a new insight into the synergistic effect of Cu 2+ and calcite on the SDZ degradation, and promotes an understanding of the environmental benefits of natural calcite.
研究领域:
环境科学
·
化学工程
·
分析化学
·
水处理
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PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2021
·
IF 3.503
Hongyan Ma,Shanshan Liu,Wenshan Qu,Qi Huang,Linyuan Li,Fujiang Chu,Yuyun Zhu,Xinlin Lv,Zhimin Wang,Jingjing Zhu
Context The traditional drying method, sun drying, for Chrysanthemum morifolium Ramat. cv. Hang-ju (Compositae) (HJ) is widely replaced by sulphur fumigation (SF), which has an unknown effect on its efficacy.Objective To investigate protective effects of nonfumigated HJ (NHJ) and sulphur-fumigated HJ (SHJ) water extracts against oxidative stress and lipid peroxidation.Materials and methods Sprague-Dawley rats were administered high-fat diet to induce hyperlipidaemia and randomly divided into eight groups (n = 6): control, fenofibrate, NHJ and SHJ extracts (1, 2 or 4 g crude drugs/kg/d; intragastric administration for 8 weeks). Serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were detected. Human umbilical vein endothelial cells (HUVECs) were treated with NHJ and SHJ extracts (50, 100 or 200 μg/mL) for 24 h, followed by oxidized low-density lipoprotein (ox-LDL, 20 μg/mL) for 2 h in vitro. Cellular reactive oxygen species (ROS), SOD and MDA levels and apoptosis were evaluated.Results NHJ was more effective than SHJ in decreasing serum TG, TC, LDL-C, LDL/HDL and MDA while increasing serum HDL-C and SOD levels at high doses. SHJ (IC50=19.9 mg/mL) suppressed HUVEC growth stronger than NHJ (IC50=186.7 mg/mL). At 200 μg/mL, NHJ was more effective than SHJ in downregulating ROS and MDA levels, reducing HUVECs apoptosis rate and elevating SOD activity in ox-LDL-treated HUVECs.Conclusions SF causes oxidative damage and attenuates antioxidative activity in ox-LDL-treated HUVECs, which promotes lipid peroxidation. SF is not recommended for processing HJ.
研究领域:
药理学
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植物化学
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DOI
PubMed
高影响因子
Antioxidants
·
2026
·Vol. 15
·期 8
·1038
·
IF 8.2
Yahui Yan,Chaoming Zhang,Yongxia Tao,Zuoshan Feng
Fruit ripening is accompanied by extensive reprogramming of secondary metabolism, which determines the antioxidant value of medicine-food homologous fruits.Ziziphus jujubaMill. cv. ‘Junzao’ (Junzao) is a high-quality cultivar rich in bioactive compounds, yet its stage-dependent metabolite accumulation and the corresponding antioxidant capacity remain poorly resolved. In this study, widely targeted metabolomics was combined with the quantification of total phenolic (TPC), total flavonoid (TFC), and total triterpenoid (TTC) contents and with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS) radical-scavenging assays to profile Junzao fruits at five developmental stages. A total of 2388 secondary metabolites were identified, with flavonoids and terpenoids representing the major classes. TPC and TFC were highest at the immature YG (young-fruit) stage, whereas TTC peaked at the BS (white-ripe) stage; all three decreased during subsequent ripening, consistent with the stronger DPPH and ABTS radical-scavenging activities observed in early-stage fruits. Multivariate analyses revealed distinct metabolic profiles among developmental stages, and 2111 differentially accumulated metabolites (DAMs) were identified. K-means clustering resolved nine temporal accumulation patterns, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicated dynamic regulation of flavonoid biosynthesis, phenylpropanoid metabolism, and triterpenoid-related pathways. Spearman correlation analyses further identified ten metabolites, comprising six flavonoids, three triterpenes, and one phenolic acid, that were strongly associated with antioxidant capacity (|r| ≥ 0.5,p< 0.05), highlighting their potential as biomarkers for quality evaluation. Overall, immature Junzao fruits exhibited superior antioxidant capacity, supporting their promise as functional-food ingredients and providing a basis for stage-specific harvesting and utilization.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Weiwei Wu,Yanming Tuo,Zhihui Wang,Na Chen,Yuxue Jiang,Chuanjiu Wu,Yongquan Xu,Wen Zeng,Weijiang Sun
Tea flowers and seeds, non-leaf organs of Camellia sinensis , are often discarded, leading to inefficient resource utilization. This study evaluated the potential of tea flowers and seeds by preparing white teas from fresh leaves (WT), flowers (WTF), and seeds (WTS) of Camellia sinensis cv. Fuding Dabaicha. Sensory evaluation, targeted metabolomics, and molecular simulations were used to investigate taste components and their impact on pleasantness. WTF exhibited the highest pleasantness score (6.60), followed by WTS (6.36) and WT (6.10). Four sugars— d -fructose, glucose, D-galactose, and D-xylose—synergistically enhanced pleasantness, with a dose-dependent increase, especially d -fructose. Molecular simulations showed these sugars formed a stable binding network with the CHRM1 receptor (−60.04 kcal/mol), with d -fructose as the key contributor. Alanine scanning identified ARG123 as a critical binding residue. This study provides support for the high-value utilization of tea flowers and seeds and offers insights into enhancing the pleasantness of tea beverages.
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高影响因子
危险品化学品经营许可证（带存储）