PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·Vol. 26
·期 15
·11498-11505
·
IF 2.9
Jiannan Sun,Ke Yan,Aizhao Pan,Pan Zhang,Xuehang Chen,Xinyi Shi,Chengyu Shi
Fluorescence nanothermometry based on quantum dots is a current research hotspot for novel non-contact temperature monitoring, and is of vital significance for the modulation and design of the sensing properties of sensors. Herein, a design strategy to modulate the temperature-sensing characteristics of quantum dots based on the thickness of a shell is proposed. In this study, CdSe/ZnS quantum dot/POSS-based temperature probe films with varying fluorescence characteristics were developed, and the influence of the ZnS shell on temperature sensing was examined by varying the thickness of the ZnS shell. The temperature dependency, linearity, range of applications, and reversibility of quantum dot thin film probes were all considerably regulated by the ZnS shell, according to research on quantum dot/POSS-based films coated with various shell thicknesses. The CdSe/ZnS temperature probe with 4 monolayers (MLs) stood out among the rest due to its strong thermal stability (at least 5 cycles), large usable temperature range (20–80 °C), and excellent temperature sensitivity (R2 > 0.994). The results demonstrated that the temperature sensing performance of quantum dots was the consequence of the combined effect of multiple temperature response properties induced by the thickness of the shell, and the shell control of quantum dots to optimize the temperature sensing performance was an essential approach for the design of temperature probes. This work demonstrates the great potential of the shell in tuning the temperature sensing performance of quantum dots and provides a viable approach for the design of quantum dot temperature probes.
研究领域:
材料科学
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纳米技术
·
传感器技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Zhiqiang Zhou,Liyun Yang,Ling Huang,Yuanping Liao,Yi Liu,Qi Xiao
The selective and quantitative detection of H 2 O 2 is important for its employment in physiological, environmental and industrial applications. In this paper, a sensitive and selective strategy for H 2 O 2 detection was established based on the fluorescence quenching of CdSe@ZnS quantum dots (QDs) by H 2 O 2 -mediated etching process of Ag nanoclusters (AgNCs). In this strategy, dihydrolipoic acid (DHLA) modified AgNCs were applied as H 2 O 2 response group, the existence of H 2 O 2 could initiate the oxidation of AgNCs and the production of Ag + . CdSe@ZnS QDs are very sensitive to Ag + , which could give rise to the effective fluorescence quenching of CdSe@ZnS QDs. Based on this strategy, the present fluorescent assay could realize the quantification detection of H 2 O 2 and the limit of detection is calculated to be 0.3 μM under the optimum conditions. Furthermore, CdSe@ZnS/AgNCs hybrid-based probe was applied to detecting H 2 O 2 in milk samples and showed a good recoveries results ranged from 95.8% to 112.0%, meaning the potential applicability of this strategy.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
Current Analytical Chemistry
·
2021
·Vol. 17
·期 7
·1027-1036
·
IF 1.892
Qiao Dan,Deng Qiliang,Wang Shuo
Background: The approach for the determination of aminoimidazo-azaarenes (AIAs) is urgently required due to their mutagenic activity and potential carcinogenicity. For this purpose, a CdSe/ZnS quantum dots (CdSe/ZnS QDs)-embedded molecularly imprinted silica gel with high selectivity
and sensitivity was successfully prepared and applied to analyze such target. Methods: Herein, CdSe/ZnS QDs acted as the fluorescent probe, and molecularly imprinted silica gel as a recognition probe was synthesized on the surface of QDs via sol-gel method. 2- Aminobenzimidazole, which
has a similar structure with AIAs, as well as low cost and low toxicity, was selected as the dummy template. Results: The material has a high imprinting factor (IF=6.15); the linear range of CdSe/ZnS@MIP as a fluorescent sensing material for AIAs is 0.5-20.0 μg L-1
with the limit of detection (LOD) of 0.25 μg L-1 (3σ/s). Moreover, the fluorescent materials have been further applied to determine AIAs in beef floss and grilled fish fillet with satisfactory recoveries (82.8-110.8 %), and the relative standard deviation (RSD)
is < 5.5%. Conclusion: The proposed CdSe/ZnS QDs-embedded molecularly imprinted silica gel can be used to easily and selectively detect AIAs.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
高影响因子
Materials Advances
·
2021
·Vol. 3
·期 1
·682-688
·
IF 0
Xin Wen,Huahua Fan,Lihong Jing,Ming Deng,Xiaodan Huang,Tifeng Jiao,Li Zhang,Minghua Liu
The design and fabrication of chiral inorganic materials with circularly polarized luminescence (CPL) have attracted great interest. Herein, achiral fluorescent quantum dots (QDs) were found to show CPL activity upon doping into the two-component hydrogels composed of N-[(9H-fluoren-9-ylmethoxy) carbonyl]-glutamic acid (Fmoc-Glu) and deoxyguanosine (dG). It was found that the helical structures could be formed in the two-component hydrogels, and the helicity was dependent on the absolute configuration of Fmoc-Glu. Upon introduction of water-soluble achiral CdSe/ZnS QDs into the hydrogel, the hydrogel could transmit chirality to QDs and make achiral QDs show obvious CPL signals. The mirrored CPL spectra of QDs were obtained when the enantiomeric Fmoc-Glu was used, i.e., negative and positive CPL signals appeared in the L-Fmoc-Glu/dG and D-Fmoc-Glu/dG hydrogels, respectively, which indicated that the CPL signal followed the molecular chirality of Fmoc-Glu. However, when K+ ions were added into the co-assembly of Fmoc-Glu/dG/QDs, only negative CPL signals of QDs could be obtained regardless of whether the L-Fmoc-Glu or D-Fmoc-Glu was used. It was suggested that the G-quadruplex (G4) was formed when K+ ions were introduced into the gel, which is more competitive in inducing the supramolecular chirality of achiral QDs than Fmoc-Glu. This study provides not only a convenient way to fabricate CPL-active QDs via gelation, but also a deep understanding on the competitive chirality transfer in the multi-component supramolecular hybrid system.
研究领域:
材料科学
·
化学
·
生物物理学
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产品关联
DOI
COLLOIDS AND SURFACES B-BIOINTERFACES
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2013
·
IF 3.554
Moyuan Cao,Lin Yu,Ping Zhang,Han Xiong,Yucun Jin,Yi Lu,Li-Qun Wang
In this research, soft-binding aminopropanol (APP) was employed as an efficient ligand, for the transfer of as-prepared hydrophobic CdSe/ZnS quantum dots (QDs) into polar solvents. It was found that the ligands at the surface of the original QDs could be completely replaced by APP after a phase-transferring process which successfully maintained fluorescence properties and original morphology of the QDs. The resulting intermediate QDs were soluble in common polar organic solvents, such as dimethyl sulfoxide (DMSO), dimethyl formamide (DMF) and tetrahydrofuran (THF), and the soft-binding ligand could be easily removed by exposure to water. Taking advantage of the excellent solubility of the APP-capped QDs and the soft-binding characteristics of APP, a novel reaction-free method was investigated for the fluorescent labeling of polysaccharide-based micelles via encapsulation of the intermediate QDs. The incorporation of QDs had little effect on the size of the micelles and did not elevate their cytotoxicity. A photo-induced fluorescence enhancement effect was observed for the incorporated QDs, and the QD-labeled micelles could be used for cell imaging. The concept of soft-binding ligand capped QDs and the reaction-free fluorescent labeling method can be applied to a wide range of QD studies.
研究领域:
材料科学
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生物医学工程
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纳米技术
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Xiaoxia Kang,Jige Yuan,Xijuan Gu,Xiaoyan Li,Tao Wen,Miao He,Yue He,Xiaoxiao Yang,Yuechen Li,Conglin Guo,Haiwei Ji,Yuling Qin,Li Wu
The agriculture and environmental industries have employed organophosphorus pesticides (OPs) chemicals as insecticides extensively in previous decades, leading to persistent life-threatening issues. Therefore, developing a platform for the efficient identification of OPs is significant for environmental protection and human health. In this study, a strategy for the colorimetric and fluorescence detection of OPs by combining quantum dots (QDs) with DNA-templated silver nanoclusters (DNA-AgNCs) was proposed. Technically, the fluorescence of DNA-AgNCs will be enhanced once guanine (G)-rich sequences get close to the DNA-AgNCs to form hybrid complexes DNA-AgNCs/G, while quenching of fluorescence can be attributed to the hydrolysate (thiocholine) of acetylthiocholine (ATch) that was caused by acetylcholinesterase (AchE). Based on the fact that OPs can irreversibly inhibit the catalytic activity of AchE and slow down the fluorescence quenching of DNA-AgNCs/G, thus, OPs detection can be achieved by evaluating the fluorescence intensity of DNA-AgNCs/G. Additionally, a dual-mode detection system (colorimetric and fluorescent) is achieved through an ion-exchange reaction between QDs and DNA-AgNCs/G. The concentration of OPs is quantified by monitoring fluorescence changes in the QDs and poly(9,9-dioctylfluorene) (PFO) polymer dots (Pdots) system, with PFO Pdots serving as a stable internal reference. Using trichlorfon as a model, the limit of detection (LOD) was 2.55 pg/mL, while an excellent linear relationship with the trichlorfon concentrations in the range of 5 × 100–5 × 105 pg/mL was obtained. Additionally, visual detection (as low as 1.06 pg/mL) can be realized readily, taking advantage of the ion-exchange reaction between DNA-AgNCs and QDs.
研究领域:
环境科学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Hao Hao,Hui-Ling Hu,Yi Liu,Feng-Lei Jiang
In this work, CdSe/ZnS core/shell quantum dots (QDs) were synthesized. Dilution of the as-synthesized QDs would lead to instability with photoluminescence (PL) quenching. It was found that the addition of tertiary phosphine and alkylthiol could recover the PL intensity of diluted QDs and make these QDs stable. Specifically, the recovery efficiency of tertiary phosphine was stronger than that of alkylthiol. Trioctylphosphine (TOP) and octanethiol (OT) were chosen as the representative ligands, respectively. Isothermal titration calorimetry (ITC) demonstrated that interactions between both TOP/OT and QDs were exothermic and spontaneous. The interaction between TOP and QDs was mainly driven by van der Waals forces due to the branched structure. This interaction had a negative entropy change, while the interaction between OT and QDs had a positive entropy change. There were about ∼12 TOP molecules and ∼17 OT molecules bound to a QD particle. The binding constants of TOP/OT to QDs were 1.6 × 103 and 12.8 × 103 M–1, respectively. The reaction rate between TOP and QDs was fast, and the addition of OT could slow the reaction rate constant from 0.081 to 0.024 min–1. PL decay indicated that TOP and OT could increase the radiative recombination of excitons. Furthermore, both TOP and OT could greatly improve the brightness of the QD films, which was a prerequisite for the efficient improvement of quantum dot light-emitting diodes. This work explicitly investigated the dilution process of QDs, explored the methods for keeping diluted QDs stable and bright, and highlighted the importance of surface ligands.
研究领域:
材料科学
·
光学
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2015
·Vol. 13
·期 1
·1-10
·
IF 4.115
Chen Jun,Yang Xiao-Quan,Qin Meng-Yao,Zhang Xiao-Shuai,Xuan Yang,Zhao Yuan-Di
Background X-ray computed tomography (CT) imaging can be used to reveal the three-dimensional structure of deep tissue with high spatial resolution. However, it cannot reveal molecular or cellular changes, and has great limitations in terms of specificity and sensitivity. Fluorescence imaging technology is one of the main methods used for the study of molecular events in vivo and has important applications in life science research. Therefore, the combination of CT and fluorescence imaging is an ideal dual-modal molecular imaging method, which can provide data on both molecular function and tissue structure, and has important research value. In a previous study, Bi2S3 nanoparticles were wrapped with quantum dots in SiO2 to generate CT and fluorescence imaging. However, this type of probe led to low survival and caused innegligible in vivo toxicity in mice. Therefore, it is necessary to develop new multifunctional probes that demonstrate biocompatibility and safety in vivo.Methods A polyethylene glycol-phospholipid bilayer structure was used to synthesize hybrid clusters containing hydrophobic Bi2S3 nanoparticles and quantum dots for combined CT/fluorescence imaging. Mean particle size, polydispersity index, and zeta potential were used to study the stability over an 8-week test period. In vivo CT and fluorescence imaging experiments were performed, and the in vivo safety of the probe was evaluated, using histopathological, biochemical, and blood analyses.Results The probe distinctly enhanced the CT contrast and had fluorescence imaging capability. In addition, the nanocomposite hybrid clusters showed a longer circulation time (>4 h) than iobitridol. The results also showed that the Bi2S3-QD@DSPE probe had good biocompatibility and safety, and did not affect normal organ functioning.Conclusion sBi2S3-QD@DSPE hybrid clusters exhibited remarkable performance in CT angiography and fluorescence imaging in vivo.
研究领域:
医学影像学
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纳米医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Wenyuan Zhao,Ying Ma,Jianshan Ye,Jiye Jin
Many quantum dots (QDs) are considered as excellent electrochemiluminescence (ECL) fluorophores with high quantum yields (QYs) and good stability. However, their ECL efficiency dramatically decreases in the aqueous solution compared to that in organic phase, which significantly limits their applications in bioanalysis. In this study, we developed a new sensing strategy via the combination of a closed bipolar electrode and electrochemiluminescence (CBP-ECL) system, which allows the separation of sensing in aqueous media and generation of ECL in organic media. Two kinds of QDs, CdSe/ZnS and CsPbBr 3 were applied as ECL fluorophores in this system, and stable, high QYs ECL signals were achieved. The resulting sensing platform demonstrated high sensitivity for the detection of H 2 O 2 with a linear range of 0.02−12 mM and the limit of detection (LOD) of 9.2 × 10 −8 M when CdSe/ZnS QDs were used as ECL reagents. We envision that this system can be widely employed in any kind of bioanalysis which can produce the electrochemical currents in the detecting cell. More importantly, we expect this system could break through the limitation of the fluorophores, especially those with solvent dependent ECL signals such as aggregation-induced emission (AIE) molecules, or most of the hydrophobic dyes.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Research Letters
·
2016
·Vol. 11
·期 1
·1-8
·
IF 2.584
Yan Xinyu,Wang Kan,Lu Wenting,Qin Weijian,Cui Daxiang,He Jinghua
Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.
研究领域:
医学诊断
·
生物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 6
·2764-2771
·
IF 2.562
Guozhen Fang,Chao Fan,Huilin Liu,Mingfei Pan,Huidan Zhu,Shuo Wang
A novel molecularly imprinted optosensing material (MIOM) based on ionic liquid (IL)-stabilized CdSe/ZnS quantum dots (QDs) was prepared, for highly selective and sensitive recognition of the mycotoxin zearalenone (ZON). ZON is expensive and highly toxic, so the ZON analog cyclodo-decanyl-2,4-dihydroxybenzoate (CDHB) was instead used as the template. MIOM was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and adsorption experiment. Under optimal conditions, the relative fluorescence intensity of MIOM decreased linearly with increasing ZON concentration, in the range of 0.003–3.12 μmol L−1. MIOM had a detection limit of 0.002 μmol L−1. MIOM was used to detect ZON in corn, rice and wheat flours, and at three concentration levels 50, 100 and 500 ng g−1, ZON recoveries for these three cereals were 94.2–98.7%, 93.1–107.6% and 84.4–106.0%, respectively.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Toxins
·
2022
·Vol. 14
·期 10
·710
·
IF 5.075
Shasha Feng,Wei Hu,Fubin Pei,Zhiwei Liu,Bin Du,Xihui Mu,Bing Liu,Qingli Hao,Wu Lei,Zhaoyang Tong
A sensitive dual-readout immunosensor for fluorescence and electrochemiluminescence (ECL) detection of ricin was established, which was combined with a streptavidin–biotin signal amplification system. CdSe/ZnS quantum dots with fine fluorescence and ECL properties were used as the dual-signal function probes of the sandwich immunocomplex. Under the optimum experimental conditions, the dual signal intensity increased significantly with the rise in ricin concentration. The fluorescence intensity of the senor exhibited a good liner relationship toward the ricin concentrations with 0.1~100 ng/mL and the limit of detection (LOD) was 81.7 pg/mL; taking ECL as the detection signal, the sensor showed a linear relationship with the ricin concentrations ranging from 0.01 ng/mL to 100 ng/mL and the LOD was 5.5 pg/mL. The constructed sensor with high sensitivity had been successfully applied to the detection of ricin in complex matrices with satisfactory recoveries. The proposed immunosensor model can be extended to the analysis and detection of others target proteins.Keywords:immunosensor;ricin;fluorescence;electrochemiluminescence;dual readout;CdSe/ZnS quantum dotsKey Contribution:A dual-readout (fluorescence and ECL) immunosensor for ricin detection was established based on CdSe/ZnS quantum dots with dual signal, which was combined with a streptavidin–biotin signal amplification system.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
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