CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Jinyu Li,Dandan Liu,Guoxin Tan,Zhinan Zhao,Xinggang Yang,Weisan Pan
To develop a potential nanocarrier for the topical ocular administration of curcumin (CUR), a novel thiolated chitosan was synthesized by the covalent binding between N -acetyl- l -cysteine (NAC) and chitosan (CS) to surface modify the nanostructured lipid carrier loaded CUR (CUR-NLC). And the superiorities of the CS-NAC co polymer coated CUR-NLC over chitosan oligosaccharides (COS) or carboxymethyl chitosan (CMCS) modification were also verified in detail. As expected, the increment in particle size and the reversal of zeta potential occurred after surface decorating, and the most prominent electropositivity was obtained for the CS-NAC-CUR-NLC group. Additionally, the utilization of the CS-NAC coating demonstrated an effectively controlled release over 72 h and attained a 6.4 and 18.8 fold increase in apparent permeability coefficients (P app ) compared with the CUR-NLC and the self-made eye drops, respectively. Meanwhile, the clearance rate of the NLC labeled with Rhodamine B was significantly delayed in the presence of CS-NAC. By contrast, CS-NAC-CUR-NLC was superior to the COS and CMCS coated ones in view of in vitro release, drug permeability and corneal retention. Moreover, the results of the in-vivo and in-vitro characteristics demonstrated that the promoting effect of CMCS coating was relatively weaker than COS coated ones. Ocular irritation test was executed on the CS-NAC-CUR-NLC, neither a sign of toxicity nor irritation to the external ocular tissues was observed. In conclusion, CS-NAC-CUR-NLC possesses a greater potential as an ocular drug-delivery system comparing with the COS-CUR-NLC and CMCS-CUR-NLC.
研究领域:
药剂学
·
生物材料
·
眼科
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DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Chao Luo,Cong Chen,Na Zhang,Jiani Li,Yaru Liang,Weibin Wu,Kai Yang
Tanshinone IIA, an important active ingredient isolated from Salvia miltiorrhiza , exhibits a broad spectrum of biological activities including anti-inflammatory, anti-oxidative, and anti-tumor. However, its poor water solubility results in low bioavailability and limits the subsequent clinical application. Chitosan nanoparticle is an excellent drug delivery carrier due to the ideal biocompatibility and pH-sensitive. Here, we developed an innovative and efficient approach to fabricate the tanshinone IIA-chitosan nanoparticles (TA/CS-NPs) by initially creating a tanshinone IIA-chitosan solid dispersion as the precursor for nanoparticle synthesis. This method successfully overcame the problem that the hydrophobic tanshinone IIA could not be directly mixed with the hydrophilic chitosan to prepare nanoparticles. The optimal conditions for TA/CS-NPs preparation were obtained through the response surface method, with the chitosan concentration of 31 mg/ml, the mass ratio of CS to TA 27:1, and stirring time 30 min. In vitro release and cell uptake experiments proved that the TA/CS-NPs had pH-sensitive drug release property. In ph5.0 dissolution medium or the medium of inflammatory cell, the nanoparticles revealed rapid drug release properties. However, in the ph7.4 dissolution medium or the medium of normal cells, the drug release rate of nanoparticles was very slow. Subsequently, cellular experiments demonstrated that the anti-inflammatory and antioxidant activities of tanshinone IIA were markedly enhanced through nanoparticle delivery. Compared with direct administration of tanshinone IIA, TA/CS-NPs have more significant activity on the inhibition of inflammatory factors (IL-1β, IL-6, TNF-α and NO), regulation of oxidative stress products (ROS, MDA) and antioxidant products (SOD, GSH, and CAT), protective effect on mitochondrial damage, inhibition of inflammatory signaling pathways (p-p65, NLRP3). This study provides a successful method to encapsulate hydrophobic drugs into hydrophilic chitosan nanoparticles and improve the therapeutic activities of the loaded drug.
研究领域:
药剂学
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生物医学工程
·
中药学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ying Lu,Xiangzhou Li,Jun Zhou,Peng Zhou,Jiajia Lai,Yanhong Yang,Liqun Shen
The unique structure of chitosan-based micelles can be loaded with essential oil, so it is significant to study the modification of chitosan and the interactions between chitosan and essential oil, while molecular dynamics (MD) simulation and density functional theory (DFT) provide a solution. In this study, three kinds of amphiphilic chitosan derivatives (CSDs) were constructed by grafting of different hydrophilic and hydrophobic groups. Amphiphilic chitosan micelles loaded with Chinese fir essential oil (CFEO) were prepared by self-assembly. The aggregation behavior of CFEO component (cedrol and α-cedrene) in the solutions of chitosan and three CSDs were simulated using MD, and the mean square displacement was calculated. DFT analyzed the mechanism for regulating the molecular properties of CSDs by different modification methods, and explored the intensity and type of interaction force between cedrol/α-cedrene and three CSDs. The results show that cedrol and α-cedrene were more easily aggregated near the modified CSDs, with CS-g-PLA showing the strongest trapping ability. The grafting of polylactic acid on chitosan resulted in a significant decrease in HOMO-LUMO energy gap and an increase in reactivity activity. Widely distributed hydrogen bonding and van der Waals forces were confirmed to be the key to enhanced loading capacity.
研究领域:
生物化学
·
材料科学
·
计算化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 4
·610
·
IF 5.1
Chengcheng Gao,Ni Zhang,Ying Chen,Fengwei Xie,Linghan Meng,Xiaozhi Tang
The strong hydrophilicity of chitosan-based films limits their practical applications. To enhance the hydrophobicity of these films, hierarchical carnauba wax particles were prepared using the Pickering emulsion method and subsequently coated onto the film surfaces. The wax was stabilized with various types and concentrations of TiO2. The resulting wax particles exhibited a micro-scale structure, with nano-scale TiO2and micro-scale TiO2aggregates present on the surface. No significant differences in contact angle were observed among these particles. Hydrophilic TiO2demonstrated smaller sliding angles and particle sizes. To improve the mechanical durability and compatibility of the wax particles with the chitosan matrix, the wax particles were mixed with a diluted chitosan solution before coating. As the concentration of wax particles increased and the concentration of chitosan solution decreased, more wax particles became exposed on the surface. This exposure increased the roughness of the coatings, resulting in a significant increase in contact angle and a decrease in sliding angle. A high concentration of chitosan provided greater protection to wax particles during mechanical durability tests. Additionally, the residue rate of liquid foods on the coating films significantly decreased. This study demonstrates that the Pickering emulsion method is an effective approach for preparing hierarchical wax particles, and that mixing these particles with a polymer similar to the matrix can effectively improve mechanical durability.
研究领域:
材料科学
·
表面化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jifang Yuan,Qian Hou,Xiaofeng He,Lingzhi Zhong,Meirong Li,Xiaobing Fu,Hongchen Liu
Wound dressing diligently facilitate healing by fostering hemostasis, immunoregulation, the angiogenesis, and collagen deposition. Our methodology entails fabricating chitosan-taurine nanoparticles (CS-Tau) through an ionic gelation method. The morphology of CS-Tau was observed utilizing Transmission electron microscopy (TEM), scanning electron microscopy (SEM) and Dynamic Light Scattering (DLS). The nanoparticles are subsequently incorporated into carboxymethyl chitosan hydrogels for crosslinking by EDC-NHS, yielding hydrogel dressings (CMCS-CS-Tau) designed to extend the duration of taurine release. In vitro investigations confirmed that these innovative compound dressings displayed superior biodegradation, biocompatibility, cytocompatibility, and non-toxicity, in addition to possessing anti-inflammatory properties, and stimulating the proliferation and mobility of human umbilical vein endothelial cells (HUVECs). Experiments conducted on mice models with full-thickness skin removal demonstrated that CMCS-CS-Tau efficaciously aided in wound healing by spurring angiogenesis, and encouraging collagen deposition. CMCS-CS-Tau can also minimize inflammation and promote collagen deposition in chronic diabetic wound. Hence, CMCS-CS-Tau promotes both acute and chronic diabetic wound healing. Furthermore, the sustained release mechanism of CMCS-CS-Tau on taurine reveals promising potential for extending its clinical utility in relation to various biological effects of taurine.
研究领域:
材料科学
·
生物医学工程
·
药剂学
·
伤口愈合
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Junhui He,Li Yang,Dongmei Li,Jiaxiu Xie,Guili Zhou,Rongfei Zhou,Yi Li,Guining Wei,Zhiqiang Gong,Li Li,Kedao Lai,Juying Zhou
Limitations of the blood-brain barrier (BBB) are responsible for the low efficacy and high toxicity of many antidepressants. However, strategies to enhance BBB targeting to improve the efficacy and safety of antidepressants remain challenging. In this study, we prepared transferrin (Tf)-functionalized chitosan (CS)- N , O -carboxymethy chitosan (NOCMS) nanosystems (NPs) (NOCMS-CS-Tf NPs), which can enhance BBB targeting in the depressive environment and allow more NPs to enter the brain. Experiments demonstrated that NOCMS-CS-Tf NPs had enhanced BBB targeting in the depressed environment compared to the normal environment and were safe and non-toxic. In vitro experiments demonstrated that NOCMS-CS-Tf NPs could target multiple cell lines in the brain and had stronger BBB targeting in neuroinflammatory and neuronal injury environments than in normal environments; in vivo experiments demonstrated that NOCMS-CS-Tf NPs had stronger BBB targeting in the brains of depressed mice than in normal mice, and were safe and non-toxic. The nanosystem enhanced BBB targeting in the setting of neuroinflammation and neuronal damage in depression, providing a promising approach to drug delivery systems for depression and bringing new hope for the return to the market of many first-line antidepressants that have been withdrawn.
研究领域:
药剂学
·
神经科学
·
生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2013
·Vol. 129
·期 6
·3185-3191
·
IF 1.395
Yu Chen,Fengju Wang,Dongran Yun,Yanwen Guo,Yanchun Ye,Yanxi Wang,Huimin Tan
In this study, we explored a new method for preparing C 6 quaternary ammonium chitosan (CTS) derivatives. The C 2 NH 2 of CTS was first protected by benzaldehyde. The C 6 OH of CTS was then transformed into a sulfonyl ester, which was then reacted with NaN 3 through nucleophilic substitution to introduce the N 3 group at the CTS C 6 position. This intermediate was reacted in a click chemistry reaction with a terminal alkynyl quaternary ammonium salt; this was followed by the deprotection of C 2 NH 2 with acid to furnish the C 6 quaternary ammonium CTS derivative. The structures and properties of synthesized products in the reactions were characterized by Fourier transform infrared spectroscopy, NMR, X-ray diffraction, and thermogravimetric analysis, respectively. The effects of the reaction conditions on the degree of Schiff-base CTS quaternization were evaluated by elemental analysis. The largest inhibition zone test and the minimum inhibitory concentration test showed that compared with CTS, the prepared CTS derivative had significantly improved antibacterial activity toward Staphylococcus aureus and Escherichia coli . © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
研究领域:
高分子化学
·
药物化学
·
生物材料
·
抗菌材料
·
天然产物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2026
·
IF 4
Linlin Wang,Lilong Wang,Jinhe Tian,Nan Wang,Chen Song,Shuang Song
BACKGROUND Chitosan (CTS) and chitosan oligosaccharides (COS) are widely used in the food industry due to their bioactive properties. However, their cationic nature enables interaction with whey protein isolate (WPI), affecting protein digestibility. Fucoidan (FUC), a naturally occurring anionic sulfated polysaccharide from brown algae, can form electrostatic complexes with CTS and COS to partially alleviate this effect; however, this process requires optimization. RESULTS Chitosan–fucoidan (CTS–FUC) and chitosan oligosaccharide–fucoidan (COS–FUC) complexes were successfully prepared and characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy, and other analytical instruments, confirming that complexation occurred via electrostatic interactions between amino groups of CTS and COS and the sulfate groups of FUC. Subsequently, the impact of these carbohydrates on WPI's digestibility was assessed during simulated gastrointestinal digestion. The CTS–FUC and COS–FUC complexes mitigated the inhibition of protein digestion caused by CTS and by COS alone. Specifically, the addition of the CTS–FUC complex increased the degree of hydrolysis of WPI by 24.6% compared with the corresponding mixed group, whereas the COS–FUC complex increased it by 22.6% compared with its corresponding mixed group. Furthermore, compared with the group containing CTS + FUC or COS + FUC, both preformed complexes increased protein concentration to 1.08-fold. The free trypsin concentration in the CTS–FUC + WPI group was 1.11 times that in the CTS + FUC + WPI group, whereas the concentration in the COS–FUC + WPI group was 1.06 times that in the COS + FUC + WPI group. Spectroscopic and chemical methods further revealed that the complexes reduced the binding of CTS and COS to protein and trypsin. CONCLUSION Overall, this study demonstrates that CTS–FUC and COS–FUC complexes effectively alleviated the negative impact of CTS and COS on protein digestion, offering new insights into mitigating their adverse nutritional effects in food systems. © 2026 Society of Chemical Industry.
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2014
·Vol. 9
·期 6
·e100743
·
IF 3.534
Zhanwei Ma,Wenyan Wang,Ying Wu,Yiming He,Tinghua Wu
Low molecular water-soluble chitosan was prepared by the depolymerization of chitosan in the presence of a series of catalysts with active W(O 2 ) sites. Both the peroxo species [W 2 O 3 (O 2 ) 4 ] 2- and {PO 4 [WO(O 2 ) 2 ] 4 } 3- showed high efficiency in the degradation of chitosan, indicating that the degradation mechanism did not follow the radical mechanism. That means •OH is not the active species, which has been proven by the fluorescence spectra. H 2 O 2 acted as an oxidant to regenerate the active W(O 2 ) sites in the depolymerization of chitosan. The developed catalyst (TBA) 3 {PO 4 [WO(O 2 ) 2 ] 4 } is recoverable.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Siyi Ju,Fenglun Zhang,Jiufang Duan,Jianxin Jiang
Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared. The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared. CS incorporation improved the mechanical properties, as the maximum tensile strength was increased to 130.55 ± 9.42 MPa. The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes. The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability. CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200–280 nm). In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus , which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.
研究领域:
材料科学
·
生物材料
·
纳米技术
·
高分子科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2021
·Vol. 13
·期 3
·338
·
IF 6.321
Ahmed M. Omer,Maha S. Ahmed,Gehan M. El-Subruiti,Randa E. Khalifa,Abdelazeem S. Eltaweil
To develop an effective pH-sensitive drug carrier, alginate (Alg), carboxymethyl chitosan (CMCs), and aminated chitosan (AmCs) derivatives were employed in this study. A simple ionic gelation technique was employed to formulate Alg-CMCs@AmCs dual polyelectrolyte complexes (PECs) microcapsules as a pH-sensitive carrier for efficient encapsulation and release of diclofenac sodium (DS) drug. The developed microcapsules were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analyzer (TGA), and scanning electron microscope (SEM). The results clarified that formation of dual PECs significantly protected Alg microcapsules from rapid disintegration at colon conditions (pH 7.4), and greatly reduced their porosity. In addition, the dual PECs microcapsules can effectively encapsulate 95.4% of DS-drug compared to 86.3 and 68.6% for Alg and Alg-CMCs microcapsules, respectively. Higher DS-release values were achieved in simulated colonic fluid [SCF; pH 7.4] compared to those obtained in simulated gastric fluid [SGF; pH 1.2]. Moreover, the drug burst release was prevented and a sustained DS-release was achieved as the AmCs concentration increased. The results confirmed also that the developed microcapsules were biodegradable in the presence of the lysozyme enzyme. These findings emphasize that the formulated pH-sensitive microcapsules could be applied for the delivery of diclofenac sodium.Keywords:alginate;carboxymethyl chitosan;aminated chitosan;pH-sensitive microcapsules;polyelectrolyte complex;drug release
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Ding Zhou,Chao Qi,Yi-Xuan Chen,Ying-Jie Zhu,Tuan-Wei Sun,Feng Chen,Chang-Qing Zhang
Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model. The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan. Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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