BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2021
·Vol. 107
·期 1
·147-153
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IF 2.151
Zhang Rui,Dong Pengyue,Sun Hongwen,Liu Chunguang
To investigate the combined effects of excess boron (B) and high salinity on the growth of freshwater algal species, Chlorella vulgaris and Microcystis aeruginosa were cultured in the medium with different B and salinities. The results show that high levels of B and salinity inhibited the growth of the two algal species. For C. vulgaris , low levels of B can alleviate the growth inhibition induced by salinity, and low levels of salinity can also relieve the growth inhibition induced by B. In contrast, high levels of salinity have little effect on B toxicity, while high levels of B aggravate salinity stress. For M. aeruginosa , salinity aggravates B toxicity, regardless of salinity levels. B supply worsens salinity stress on M. aeruginosa , regardless of supply doses. These results suggest that it may be possible to control algal bloom by regulating B or salinities.
研究领域:
生态学
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环境科学
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2017
·
IF 5.651
Yang Peng,Aosong Deng,Xun Gong,Xiaomin Li,Yang Zhang
Considering the high concentration of mercury in industrial wastewater, such as coal-fired power plants and gold mining wastewater, this research study investigated the coupling process of lipid production and mercury bioremediation using microalgae cells. Chlorella vulgaris modified by biomimetic mineralization. The cultivation was divided in two stages: a natural cultivation for 7 days and 5 days of Hg 2+ addition (10–100 μg/L) for cultivation at different pH values (4–7) after inoculation. Next, the harvested cells were eluted, and lipid was extracted. The fluorescein diacetate (FDA) dye tests demonstrated that the mineralized layer enhanced the biological activity of microalgae cells in Hg 2+ contaminated media. Hg distribution tests showed that the Hg removal capacity of modified cells was increased from 62.85% to 94.74%, and 88.72% of eluted Hg 2+ concentration was observed in modified cells compared to 48.42% of raw cells, implying that more mercury was transferred from lipid and residuals into elutable forms.
研究领域:
环境生物学
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微生物学
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DOI
高影响因子
RENEWABLE ENERGY
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2021
·
IF 8.001
Gul Muhammad,Ange Douglas Potchamyou Ngatcha,Yongkun Lv,Wenlong Xiong,Yaser A. El-Badry,Eylem Asmatulu,Jingliang Xu,Md Asraful Alam
This study investigates modeling and optimal conditions for biodiesel production from exceedingly wet microalgae Chlorella pyrenoidosa using the catalyst, hydrochloric acid . Three levels of Box-Behnken design response surface methodology were used to optimize individual and interactive effects of parameter time (120–240 min), temperature (120–160 °C), solvent-to-wet biomass ratio (2.0–4.67), and hydrochloric acid concentration (2–4 M). Temperature was the most significant factor for direct transesterification of wet microalgae (low p-value (0.0001) and high F-value (53.89). The highest yield (19.90%) of fatty acid methyl ester was obtained on dry biomass weight basis under the optimum conditions of 240 min, 146 °C, 2.83 (vol/wt), and 3.86 M acid concentration. The artificial neural network and response surface methodology were trained with Box-Behnken design data to predict responses, and to develop and compare each model's predictive abilities . The accuracy of results indicates that both models predict the experimental data for fatty acid methyl ester yields with high correlation coefficients (R 2 ) 0.94 and 0.92, respectively for artificial neural network and response surface methodology. The potential for producing biodiesel from C. pyrenoidosa is validated by the high yields of C18 fatty acid methyl esters. Experimental analysis demonstrated biodiesel quality in comparison with European and US standards.
研究领域:
生物工程
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可再生能源
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高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Wenqi Qin,Siqi Zhang,Shujuan Yang,Feilin Lin,Qichang Zhou,Houkui Gong,Xinyu Zhao,Furui He,Yang Zhou,Yuhong Feng,Gaobo Yu,Yuanyuan Liu,Jiacheng Li
Microalgae have been known as a promising sustainable resource for bioenergy cleaner production. Herein, a series of highly dispersible novel magnetic quaternary ammonium cationic surfactants (Fe 3 O 4 @DQn, n = 1, 2, 3) for the efficient harvesting of oleaginous Chlorella pyrenoidosa ( C. pyrenoidosa ) have been investigated. The Fe 3 O 4 @DQn capture microalgae process can be divided into a three-step operation (i.e., flocculation , separation, and recycle). For harvesting C. pyrenoidosa , the Fe 3 O 4 @DQn has both a high recovery efficiency (>98.4%) and a short flocculation time (2 min). In addition, the reused Fe 3 O 4 @DQ2 can maintain a high recovery efficiency (92.31%) with five cycling times. Moreover, the N -[3-(Trimethoxysilyl)propyl]ethylenediamine (TMPED) amount and the pH value both have a positive influence on the C. pyrenoidosa harvesting. It is worth noting that the dual effect of quaternary ammonium cationic surfactant, strong positively charged and hydrophobic properties, leads to a higher harvesting efficiency of microalgae. Furthermore, the results of particle size distribution and turbiscan stability index (TSI) show that a higher specific surface area, a smaller polydispersity index and TSI value of the Fe 3 O 4 @DQn can make a more excellent adsorption and dispersion properties. Finally, the Fe 3 O 4 @DQn might provide a promising high cost-performance to harvesting of oleaginous microalgae due to the better economic viability.
研究领域:
生物技术
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环境科学
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DOI
高影响因子
ENVIRONMENTAL RESEARCH
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2022
·
IF 8.431
Shuai Wu,Jingmiao Zhang,Ao Xia,Yun Huang,Xianqing Zhu,Xun Zhu,Qiang Liao
Microalgae-based technology provides a potential approach to biologically treating oxytetracycline (OTC) wastewater due to its environmental friendliness, low cost, and high efficiency. However, the OTC degradation and transformation characteristics by microalgae are still unclear and need further exploration. This study used microalgae Chlorella sorokiniana MB-1 for OTC wastewater treatment . The OTC with an initial concentration less than 50 mg L −1 promoted microalgae growth, while OTC with a concentration higher than 100 mg L −1 inhibited microalgae growth significantly. More than 99% OTC was removed with the biomass productivity up to 1.8 g L −1 when treated OTC with 10 mg L −1 initial concentration for 7 days. Chlorophyll and total sugar contents decreased, while protein and lipid contents increased compared to the control without OTC. The malondialdehyde content firstly reduced but subsequently enhanced when increased OTC concentration, while superoxide dismutase content gradually enhanced, manifesting that traces of OTC stimulate microalgae antioxidant capacity, while the increasing OTC caused further oxidative damage to microalgae cells. The removal pathways of OTC mainly include photolysis (75.8%), biodegradation (17.8%), biosorption (3.6%), and hydrolysis (2.7%). Overall, removing OTC by microalgae was confirmed to be an excellent technology for treating antibiotics wastewater whilst accumulating microalgae biomass.
研究领域:
环境工程
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生物技术
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废水处理
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PubMed
高影响因子
International Journal of Environmental Research and Public Health
·
2023
·Vol. 20
·期 4
·3623
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IF 4.614
Junrong Li,Yingjun Wang,Ziqi Fan,Panyang Tang,Mengting Wu,Hong Xiao,Zhenxing Zeng
Antibiotics have become a new kind of organic pollutant as they are widely used in the water environment of China. Tetracycline (TC) is a class of broad-spectrum antibiotics produced or semi-synthesized by actinomycetes. Metronidazole (MTZ) is the first generation of typical nitroimidazoles. The content of nitroimidazoles is relatively high in medical wastewater, and their ecotoxicity is worthy of attention because they are difficult to completely eliminate. In this paper, the effects of TC and MTZ on the growth, cell morphology, extracellular polymer and oxidative stress ofChlorella pyrenoidosa(C. pyrenoidosa) were studied, and the toxic interactions between TC and MTZ mixture components were analyzed. The results showed that the 96h-EC50of TC and MTZ was 8.72 mg/L and 45.125 mg/L, respectively. The toxicity of TC toC. pyrenoidosawas higher than that of MTZ, and the combined toxicity effect of TC and MTZ was synergistic after the combined action of a 1:1 toxicity ratio. In addition, the algal cells ofC. pyrenoidosadied to varying degrees, the membrane permeability of algal cells was increased, the membrane was damaged, the surface of algal cells exposed to higher concentration of pollutants was wrinkled, and their morphology was changed. The extracellular polymer ofC. pyrenoidosawas affected by a change in concentration. The effect of pollutants on the reactive oxygen species (ROS) level and malondialdehyde (MDA) content ofC. pyrenoidosaalso had an obvious dose–effect relationship. This study contributes to the assessment of the possible ecological risks to green algae due to the presence of TC and MTZ in aquatic environments.Keywords:tetracycline;metronidazole;Chlorella pyrenoidosa;single toxicity;combined toxicity
研究领域:
环境科学
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毒理学
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PubMed
高影响因子
Acta Pharmaceutica Sinica B
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2022
·
IF 14.903
Hangyi Wu,Pei Yang,Aiqin Li,Xin Jin,Zhenhai Zhang,HuiXia Lv
Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments, such as high-glucose, excessive reactive oxygen species (ROS), hypoxia and malnutrition. Unfortunately, few strategies have been developed to ameliorate the multiple microenvironments simultaneously. In this study, Chlorella sp. (Chlorella) hydrogels were prepared against diabetic wounds. In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis, actively consume glucose and deplete ROS with the inherent antioxidants, during the daytime. At night, Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents. It was verified in vitro that the inactivated-Chlorella could supply nutrition, relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration. The advantages of living Chlorella and its contents were integrated ingeniously. The abovementioned functions were proven to accelerate cell proliferation, migration and angiogenesis in vitro . Then, streptozotocin-induced diabetic mice were employed for further validation. The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments, including hypoxia, high-glucose, excessive-ROS and chronic inflammation, thereby synergistically promoting tissue regeneration. Given the results above, Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.
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PubMed
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CHEMICAL ENGINEERING & TECHNOLOGY
·
2013
·Vol. 36
·期 5
·733-739
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IF 1.366
C. Prommuak,P. Pavasant,A. T. Quitain,M. Goto,A. Shotipruk
Biodiesel and valuable free lutein were demonstrated to be simultaneously produced from Chlorella vulgaris lipid extracts. The alkali catalyst used in the transesterification of triglycerides acted as a reactant in converting lutein fatty acid esters to free lutein. A maximum biodiesel yield of 33.6 % by weight of the algal lipids was obtained after a 4-h reaction with MeOH at the MeOH/biomass ratio of 16:1 using 6 % alkali catalyst. The excess of alkali and MeOH employed in the production of biodiesel ensured the complete saponification of all lutein fatty acid esters to free lutein, giving a maximum yield of 2.3 % by weight of the algal lipids. In addition, a process for the separation of the biodiesel and free lutein products from the reaction mixture is proposed. Finally, a preliminary economic assessment was conducted, the results of which suggest that the process for the simultaneous production of biodiesel and lutein from C. vulgaris may be economically feasible.
研究领域:
生物工程
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能源生物学
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植物化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Qian Feng,Yabo Sun,An Li,Xiangang Lin,Tao Lu,Dan Ding,Menghan Shi,Yingqiang Sun,Yupeng Yuan
Booming graphitic carbon nitride (g-C 3 N 4 ) photocatalyzed water splitting increases crisis of aquatic contamination. However, a controversial understanding regarding effect of g-C 3 N 4 on growth of microalgae still exists. Accordingly, Chlorella vulgaris were cultured in 0–250 mg/L of g-C 3 N 4 with biomass named as C-0, C-50, C-100, C-150, C-200, and C-250, respectively. g-C 3 N 4 below 200 mg/L was beneficial to short-term cultivation of microalgae, while it was harmful to long-time cultivation. Protein factions of C-0, C-100, and C-250 were 41.4, 42.3, and 36.4 wt%, while their lipid factions varied from 21.5, 16.9, to 17.8 wt%, respectively. In short-term cultivation, superoxide dismutase’s activity of C-0, C-150, and C-250 increased dramatically, while accumulated H 2 O 2 led to increased activity of catalase . However, it started to decrease once antioxidant enzymes were per-oxidized, leading to increase of malondialdehyde content. In long-term cultivation, activities of superoxide dismutase, catalase and malondialdehyde content decreased dramatically owning to peroxidation of algae. Scavenger tests with tertiary butanol and triethanolamine implied that·OH was dominate parameter affecting growth of microalgae. This work indicates that g-C 3 N 4 below 200 mg/L is propitious to short-term cultivation of microalgae, while it is bad to long-time cultivation of microalgae, revealing dual rules of g-C 3 N 4 in Chlorella vulgaris cultivation.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2021
·
IF 3.978
Zhikang Deng,Jinyao Zhu,Lie Yang,Zulin Zhang,Bolin Li,Ling Xia,Li Wu
The widespread use of neonicotinoid pesticides has caused environmental threat that has attracted scientific concern. This study developed a microalgae fuel cells (MFCs) with Chlorella sp. as the cathode synergistically enhanced the removal of imidacloprid (IMI). The MFCs were operated at open-circuit and closed-circuit. Under closed-circuit condition (with an external load), 57.20–61.66% of IMI (10–50 mg/L) was removed within 12 days. However, only 39.66–42.66% was removed in the open-circuit (without an external load). Additionally, a voltage of 200 mV was also detected in the closed-circuit MFCs, indicating the green bioenergy was produced during the degradation of IMI. Cyclic voltammetry revealed that the cathodic electroactivity was higher with the addition of IMI. Biomass accumulation (indicated by chlorophyll and dry cell weight) were significantly enhanced by IMI. On the 12th day, there was no difference in MDA content between the different IMI concentration (10–50 mg/L), while a significantly upregulated superoxide dismutase (SOD) of each group was also found at the same time. Six intermediates were detected and five major degradation pathways were proposed. Based on the identified products and microalgae growth, the degradation products of IMI were less toxic /non-toxic than IMI. Considering the efficient removal of IMI and energy production, the MFCs could be a promising technique for controlling IMI contamination by effective detoxification.
研究领域:
环境科学
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生物技术
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电化学
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毒理学
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2016
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IF 4.694
Wenjun Zhou,Lili Gao,Wentao Cheng,Lin Chen,Junfeng Wang,Hui Wang,Wei Zhang,Tianzhong Liu
Harvest of algal biomass from dilute culture medium at low cost remains a major hurdle to industrial scale processing. Conventional harvesting methods are not only costly, but also affect any later downstream processes partly because of the metal ions contamination by flocculants . This work constructed an electro-flotation technique including two steps of flocculation by chitosan first and then sequential bubbling flotation by non-sacrificial graphite electrodes . Laboratory work has been carried out to investigate the influences of chitosan dosage, pH value, cell density of algal culture, stirring rate and mixing time on flocculation efficiency, and the voltage and current intensity imposed on electrodes and the space between electrodes on flotation efficiency. Moreover, a 1000 L pilot bench of electro-flotator constructed to harvest Chlorella sp. was tested. Around 90% of total recovery efficiency and 50 times concentration rate have been reached, and only 23.7 g chitosan and 0.43 kWh of electricity are required to harvest 1 kg biomass. Above results proved electro-flotation by graphite electrodes assisted with chitosan is a safe and cost effective approach for microalgae harvesting.
研究领域:
环境工程
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生物技术
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微藻生物炼制
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水处理
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2019
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IF 2.87
Jiejun Xi,Jian Shao,Yuan Wang,Xiaoqin Wang,Hui Yang,Xinwei Zhang,Dongmei Xiong
Triflumizole is one of imidazole fungicides that works by inhibiting ergosterol biosynthesis , and is widely used for the control of powdery mildew and scabs on various fruits and crops. Triflumizole residue has been frequently detected in soil and aquatic ecosystems . While many studies have focused on its toxic effect on terrestrial and aquatic animals, little attention has been paid to aquatic algae, the primary producers of aquatic environments . Therefore, we evaluated the acute (96 h) toxicity effects of triflumizole on the freshwater algae Chlorella vulgaris , by examining growth, cell morphology, photosynthesis , and oxidative stress . The results showed that the 96 h median inhibition concentration (96 h-EC 50 ) was 0.82 mg / L (95% confidential interval 0.70–0.98 mg/L).The growth of algal cells was conspicuously inhibited by triflumizole exposure, and the cell surfaces appeared to be shrunkThe chlorophyll content (including Chl-a, Chl-b and T-Chl) dramatically decreased at triflumizole concentrations of 0.2 and 1.0 mg/L. In addition, the transcript abundance of photosynthesis-related genes ( psa B, psb C and rbc L) showed obvious decreases in above treatments after 96 h of exposure to triflumizole. Moreover, the algal growth inhibition was accompanied by an increase in intracellular reactive oxygen species and malondialdehyde content, as well as increased activity of antioxidant enzymes such as superoxide dismutase and peroxidase , indicating oxidative stress and lipid peroxidation . Our findings reveal that triflumizole has potential toxicity to the primary producers (freshwater algae) in aquatic ecosystems.
研究领域:
环境毒理学
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水生生态学
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植物学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）