Journal of Environmental Chemical Engineering
·
2025
·Vol. 14
·期 1
·120692
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IF 7.2
Xiaojie Huang,Nengwu Zhu,Mingyi Xu,Xiaorong Wei,Fei Li,Yong Jin,Xinyi Liao,Pingxiao Wu
Photosynthetic microbial CO 2 fixation represents an efficient strategy for industrial flue gas mitigation. However, system performance is critically dependent on the gas bubble size within the bioreactor. While large bubbles limit gas-liquid mass transfer, leaving substantial carbon unabsorbed and unconverted, excessively small bubbles can potentially impair microbial viability and activity. In this case, this study systematically investigates the trade-off in flue gas bubble size for carbon fixation by Chlorella pyrenoidosa , specifically elucidating the mechanisms by which fine bubbles impact cellular functions. The use of flue gas bubble with mean diameters of 259, 485, and 653 μm resulted in carbon fixation rates of 0.8651, 1.0175, and 0.7956 g/L/h, respectively. Notably, the peak performance at 485 μm was also associated with the highest levels of protein (59.36 %) and chlorophyll (3.483 %). Mechanistic analysis indicated that 485 μm bubbles provided an optimal balance, enhancing mass transfer compared to larger bubbles while avoiding the severe cell membrane damage and lipid peroxidation induced by exposure to 259 μm bubbles. At this optimal size, carbon metabolism was accelerated through the upregulation of key enzymes (e.g., carbonic anhydrase and RuBisCO) and enhanced energy supply (e.g., NADPH and ATP), whereas 259 μm bubbles caused metabolic suppression. These findings establish bubble size as a decisive factor governing carbon fixation efficiency in photosynthetic biohybrid systems, providing a mechanistic basis for enhancing their efficacy in flue gas mitigation.
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International Journal of Environmental Research and Public Health
·
2019
·Vol. 16
·期 20
·
IF 2.468
Xiao Tan,Kaiwen Dai,Keshab Parajuli,Xiaoshuai Hang,Zhipeng Duan,Yue Hu
The demand for phenolic compounds has been increasing rapidly, which has intensified the production and usage of phenol at a commercial scale. In some polluted water bodies, phenol has become one of the typical aromatic contaminants. Such water bodies are inescapably influenced by nutrients from human activities, and also suffer from nuisance cyanobacterial blooms. While phenolic pollution threatens water safety and ecological balance, algal cells are ubiquitous and sensitive to pollutants. Therefore, effects of phenolic pollution on interspecific competition between a bloom-forming cyanobacterium and other common alga merit quantitative investigation. In this study, the effects of phenol onMicrocystis aeruginosa(M. aeruginosa, a bloom-forming cyanobacterium) andChlorella pyrenoidosa(C. pyrenoidosa, a ubiquitous green alga) were analyzed in mono- and co-cultures. The two species were exposed to a series of phenol treatments (0, 2, 20, and 200 μg mL−1). Population dynamics were measured by a flow cytometer and analyzed by the Lotka-Volterra model. The results showed thatM. aeruginosawas more sensitive to phenol (EC50= 80.8 ± 0.16 μg mL−1) compared toC. pyrenoidosa(EC50= 631.4 ± 0.41 μg mL−1) in mono-cultures.M. aeruginosawon in the co-cultures when phenol was below or equal to 20 μg mL−1, whileC. pyrenoidosabecame the dominant species in the 200 μg mL−1treatment. Photosynthetic activity was measured by a fluometer. Results showed phenol significantly impacted the photosynthetic activity ofM. aeruginosaby inhibiting the acceptor side of its photosystem II (PSII), while such inhibition inC. pyrenoidosawas only observed in the highest phenol treatment (200 μg mL−1). This study provides a better understanding for predicting the succession of algal community structure in water bodies susceptible to phenolic contamination. Moreover, it reveals the mechanism on photosynthetic responses of these two species under phenolic stress.
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Foods
·
2022
·Vol. 11
·期 5
·725
·
IF 5.561
Kunling Lv,Qingxia Yuan,Hong Li,Tingting Li,Haiqiong Ma,Chenghai Gao,Siyuan Zhang,Yonghong Liu,Longyan Zhao
This study was conducted to investigate the prebiotic potential ofChlorella pyrenoidosapolysaccharides to provide useful information for developingC. pyrenoidosaas a green healthy food.C. pyrenoidosapolysaccharides were prepared and their physicochemical characteristics were determined. The digestibility and fermentation characteristics ofC. pyrenoidosapolysaccharides were evaluated using in vitro models. The results revealed thatC. pyrenoidosapolysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.C. pyrenoidosapolysaccharides could not be degraded under saliva and the gastrointestinal conditions. However, the molecular weight and contents of residual carbohydrates and reducing sugars ofC. pyrenoidosapolysaccharides were significantly reduced after fecal fermentation at a moderate speed. Notably,C. pyrenoidosapolysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes. Intriguingly,C. pyrenoidosapolysaccharides can promote growth ofParabacteroides distasonisand increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases. Thus, these results suggested thatC. pyrenoidosapolysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.Keywords:Chlorella pyrenoidosa;polysaccharides;fermentation;gut microbiota;prebiotic
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Materials Research Express
·
2019
·Vol. 6
·期 9
·0950c3
·
IF 1.449
Qianzhu Mu,Yanfeng Sun,Aiying Guo,Xiazhao Yu,Xiaoyue Xu,Aijun Cai,Xiuping Wang
A series of Fe3O4–TiO2 composites were successfully synthesized using Chlorella pyrenoidosa as a bio-template. C. pyrenoidosa is not only a promising bio-template for obtaining Fe3O4 and TiO2 nanoparticles (NPs) but is also a natural dye sensitizer source that improves the photocatalytic activity of the TiO2 NPs under visible light. The bandgap energy of Fe3O4–TiO2 is narrower than that of TiO2 due to the coupling between the Fe3O4 and TiO2 NPs. The coupling and dye-sensitization lead to the rapid separation and suppressed recombination of electron-hole pairs in Fe3O4–TiO2, which enhances the photocatalytic activity of TiO2 under visible light. The photocatalytic activity of the as-prepared composites was evaluated by degrading a rhodamine B (RhB) aqueous solution under visible-light irradiation. Fe3O4–TiO2 exhibits a high degradation efficiency for RhB, and can be withdrawn from solution easily using an external magnetic field.
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ACS Food Science & Technology
·
2024
·
IF 2.6
Kaini Chen,Qinbo Jiang,Hui Zhang
A Camellia oil-based Oleogel, as a plant-based alternative to fats, was obtained by freeze-drying and shearing of Chlorella pyrenoidosa protein microgel particles (CPP MPs) and guar gum costabilized Pickering emulsions. CPP MPs (pH = 5.0 to 9.0) were prepared by a top-down method, possessing particle sizes ranging from 977.00 to 569.10 nm. The high desorption energy made CPP MPs stable adsorption. Pickering emulsions were prepared by two-step emulsification. Changes in pH (5.0–9.0) and oil phase fraction (φ, 50–75%) regulated the droplet size and rheological properties, enhancing the structural stability. Fourier transform infrared (FTIR) indicated hydrogen bonds occurred in oleogels and the effects of pH on the secondary structure of CPP MPs. MPs with higher pH provided oleogels with higher modulus, thermal stability, textural properties, oil-binding capacity, and oxidative stability but lower thixotropic recovery. These findings further promote the diversification of plant-based oleogels as fat simulants for food applications.
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ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Xibo Lu,Zhuang Wang
The understanding of the environmental consequences resulting from the presence of micro(nano)plastics and carbon nanofibers (CNFs) in aquatic ecosystems is currently limited. This research endeavor sought to investigate the underlying molecular mechanisms by which engineered polystyrene-based microplastics (MPs)/nanoplastics (NPs) and CNFs, both individually and in combination, elicit toxic effects on an algal species Chlorella pyrenoidosa . The findings revealed that the combined toxicity of MPs/NPs and CNFs depended on the concentration of the mixture. As the concentration increased, the combined toxicity of MPs/NPs and CNFs was significantly greater than the toxicity of each component on its own. Furthermore, the combined toxicity of NPs and CNFs was higher than that of MPs and CNFs. The study integrated data on cell membrane integrity, oxidative stress , and antioxidant modulation to create an Integrated Biomarker Response index, which demonstrated that the co-exposure of algae to NPs and CNFs resulted in more severe cellular stress compared to exposure to NPs alone. Similarly, the combination of NPs and CNFs caused greater cellular stress than the combination of MPs and CNFs. Additionally, significant changes in the expression of stress-related genes caused by MPs/NPs alone and in combination with CNFs indicated that oxidative stress response, glucose metabolism, and energy metabolism played critical roles in particle-induced toxicity. Overall, this study provides the first insight into the toxicological mechanism of MPs/NPs and CNFs mixtures at the molecular level in freshwater microalgae.
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AQUATIC TOXICOLOGY
·
2025
·
IF 4.3
Feifan Wu,Xiangjie Pan,Yuhao Zhou,Yan Zhu,Kai Liu,Wei Li,Jiangang Han
Emerging pollutants such as antibiotics have raised great concern in recent years, but the complex coexistence of multiple antibiotics in the environment poses a new challenge in the accurate assessment of the toxicity of antibiotics to aquatic organisms such as microalgae. In this study, the mechanism of action of a combination of erythromycin (ERY) and roxithromycin (ROX) on Chlorella pyrenoidosa was illustrated based on the physiological–biochemical response and transcriptomic analysis. The results revealed an inhibitory effect on the biomass of C. pyrenoidosa at 14 d in all treatment groups, whereas an antagonistic effect was observed in the coexposure groups. The photosystem was the main target despite the existence of multiple compensatory mechanisms, such as expanding the antenna size and initiating alternative electron carriers. The intercept of electrons on the donor side of PSI limited the production of energy, whereas the adjustment of the content and ratio of pigments strengthened microalgal adaptation. Enzymes and genes related to the degradation of exogenous compounds, including cytochrome P450 (P450), glutathione S-transferase (GST) and ABC transporters, mediated the detoxification of antibiotics. The upregulated expression of related genes induced by coexposure increased resistance and explained the antagonistic effects. The shift in energy allocation by increasing the proportion of lipids met the urgent requirements of microalgal physiological activities. This study reemphasizes the modes of interactions between multiple antibiotics and provides new insights into the mechanisms of antagonism induced by combinations of antibiotics.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Zhiyi Chen,Xingyue Jiang,Guizhao Liang
CA modulates structures and properties of CPPH via non-covalent binding. • AOPs reveal key electron/H-donating sites underlying complex reactivity. • SAR of AOPs-CA explains assay-dependent synergy patterns in ABTS/DPPH/FRAP. • Quantum parameters link weak binding to reactivity enhancement. • PLR-CA shows potential synergistic cytoprotection in HepG2 cells. Non-covalent complexation with chlorogenic acid (CA) was investigated to remodel complex-level structure-activity relationships of antioxidant peptides (AOPs) from Chlorella pyrenoidosa protein hydrolysates (CPPH) and to evaluate their potential cytoprotective interaction. CPPH-CA complex formation and conformational/microenvironmental changes were characterized by FTIR, intrinsic fluorescence, UV–vis, and interfacial/colloidal measurements. Antioxidant activity and synergy of CPPH-CA and peptide-CA systems were quantified using ABTS, DPPH, and FRAP assays. Representative AOPs were identified by LC-MS/MS and in silico screening, synthesized, and analyzed by quantum chemical parameters (HOMO/LUMO, ESP, ALIE, Fukui functions and IGMH). CA associated with AOPs mainly through hydrogen bonding and van der Waals forces, inducing charge redistribution and decreased HOMO-LUMO gaps, consistent with enhanced reactivity and synergy. In H 2 O 2 -injured HepG2 cells, PLR-CA exhibited a potential cytoprotective effect under the tested conditions by improving cell viability, reducing lipid peroxidation, and restoring antioxidant enzyme activities.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shasha Zhao,Jin Qian,Bianhe Lu,Sijing Tang,Yuxuan He,Yin Liu,Yitong Yan,Shuai Jin
Microalgae-bacteria consortia (MBC) system has been shown to enhance the efficiency of microalgae in wastewater treatment, yet its effectiveness in treating levofloxacin (LEV) wastewater remains unexplored. This study compared the treatment of LEV wastewater using pure Chlorella pyrenoidosa (PA) and its MBC constructed with activated sludge bacteria. The results showed that MBC improved the removal efficiency of LEV from 3.50–5.41 % to 33.62–57.20 % by enhancing the growth metabolism of microalgae. The MBC increased microalgae biomass and extracellular polymeric substance (EPS) secretion, yet reduced photosynthetic pigment content compared to the PA. At the phylum level, Proteobacteria and Actinobacteriota are the major bacteria in MBC. Furthermore, the transcriptome reveals that the growth-promoting effects of MBC are associated with the up-regulation of genes encoding the glycolysis, the citrate cycle (TCA cycle), and the pentose phosphate pathway. Enhanced carbon fixation, coupled with down-regulation of photosynthetic electron transfer processes, suggests an energy allocation mechanism within MBC. The up-regulation of porphyrin and arachidonic acid metabolism, along with the expression of genes encoding LEV-degrading enzymes, provides evidence of MBC’s superior tolerance to and degradation of LEV. Overall, these findings lead to a better understanding of the underlying mechanisms through which MBC outperforms PA in treating LEV wastewater.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Abdulrahman AL-Ammari,Lei Zhang,Jiazhi Yang,Feng Wei,Chuntao Chen,Dongping Sun
This study aims to assess the toxicity of the commonly-spread titanium dioxide nanoparticles (TiO 2 NPs) by evaluating the exposure impact of the particles on both freshwater algae Chlorella pyrenoidosa and zebrafish liver cell line (ZFL), the two common in vitro models in toxicological studies. To compare the toxic effects of TiO 2 NPs with different physiochemical properties, three types of manufactured TiO 2 were used: bulk TiO 2 , Degussa P25 TiO 2 , and ultrafine TiO 2 NPs. Both short and long-term biological responses of green algae, such as the effect on the cell growth rate, pigment autofluorescence, and esterase activity were investigated. The dosage, physical property of TiO 2 particles, and their interactions with algal cells affect cellular growth, especially after short-term exposure. The hydrodynamic size plays a critical role in determining the acute toxicity to C. pyrenoidosa in terms of autofluorescence and esterase activity, while all types of TiO 2 NPs show toxic effects after exposure for 14 days. However, this observation is not seen when studying the effect of introduced particles in ZFL, for the precipitated Degussa P25 TiO 2 showed the highest cellular inhibition. Interestingly, despite the obvious overall toxicity toward C. pyrenoidosa , the photocatalytical properties of TiO 2 NPs may contribute to the enhanced photosynthesis in the low concentration range (< 40 µg mL −1 ). Overall, we found that the physical interactions between TiO 2 particles and the cells, particles’ size and dispersibility play critical role in the cytotoxic effect for both algal and ZFL cells, while the photocatalytical properties of TiO 2 particles may produce mixed effects on the cytotoxicity of green algae.
研究领域:
环境毒理学
·
纳米生物学
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jia Jia,Qian Liu,Chenxi Wu
Despite that the phycosphere was an important niche for the proliferation of various bacteria and antibiotic resistance genes (ARGs), the factors that affect the colonization of bacteria and ARGs in the phycosphere are still poorly understood. In this study, sterile C. pyrenoidosa co-cultured with bacteria from different sources and provided with polylactic acid microplastic (PLA MPs) and florfenicol (FF) was examined. Results showed that bacteria promoted the growth of C. pyrenoidosa and increased its chlorophyll contents. PLA MPs and FF also showed positive effects on C. pyrenoidosa due to the “Hormesis effect”. The occurrence of bacteria in the phycosphere was significantly affected by their sources and the addition of PLA MPs and FF. However, the core microbiota of the phycosphere in each group was similar. Additionally, PLA MPs and FF proliferated the abundance of phenicol-related ARGs (especially flo R) and mobile genetic elements in the phycosphere. Notably, PLA MPs and FF enhanced the abundance of Flavobacterium , a potential host of ARGs. Our results highlighted the important roles of bacteria in microalgae and demonstrated exogenous pollutants could promote the spread of ARGs between surrounding environments and the phycosphere, which provide new insights into the occurrence and spread of ARGs in the phycosphere.
研究领域:
环境微生物学
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水生生态学
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环境污染
·
微生物生态学
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ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Wei Song,Caixia Fu,Yuning Fang,Zhuoyue Wang,Ji Li,Xiaolei Zhang,Kalpana Bhatt,Lu Liu,Ningjie Wang,Fang Liu,Shunni Zhu
Microplastics (MPs), an emerging pollutant, have been increasingly raising concern due to the potential impacts on aquatic organisms. Moreover, the environmental aged MPs always exhibit different environmental behavior and interaction effect with organic pollutants from virgin MPs. In this work, the single and combined toxicity impact on Chlorella pyrenoidosa , a symbiont representative, has been investigated between MPs (e.g., polyamide microplastic (PA6), 75 μm) and organic pollutants (e.g., sulfamethoxazole (SMX) and dicamba (DCB)). Growth inhibition, chlorophyll accumulation, superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were investigated with the primary or UV-aged PA6. Above 0.5 g/L PA6 (primary or UV-aged) inhibited cell growth and chlorophyll accumulation after 96 h cultivation as compared with the control. Besides, the inhibition impacts have enhanced as the UV-aging time extending in the single PA6 systems. The algae growth inhibition rate after 96 h cultivation in both the system i.e., single (PA6: 6.9%) and combined (PA6-SMX: 14.2%, PA6-DCB: 14.9%) was slightly lower than that of exposing in organic pollutants alone (SMX: 23.9%, DCB: 25.0%), while the chl. b concentration in 60 days UV-aged PA6 combined with SMX (1.19 mg/L) or DCB (1.40 mg/L) systems were higher than in single SMX (1.04 mg/L) or DCB (1.33 mg/L) system. In addition, there were several differences of the cellular oxidative stress in the combined system of SMX and DCB. Specially, it was not noticeable of three enzymatic activities for SMX exposing in the presence of primary or UV-aged PA6. While SOD, CAT, and MDA activities was obviously increasing after exposing in PA6 and DCB combined system, indicating the significant synergistic effect on algae cells damage. This research verified the remarkable combined toxicity between UV-aged MPs and organic pollutants on microalgae.
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