NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 33
·16177-16182
·
IF 3.925
Shuyan Xiao,Liang Sun,Jiping Lu,Zhongping Dong
A label-free fluorescent biosensor based on the clenbuterol binding aptamer and the fluorescent intercalator SYBR Green I (SGI) was established for the sensitive and selective detection of clenbuterol. In the absence of clenbuterol, a weak fluorescence signal of SGI was observed. In the presence of clenbuterol, the specific recognition and high-affinity binding of the aptamer with clenbuterol induced a conformational change of the aptamer, and a longer double-stranded DNA was formed. As a result, an enhancement in the fluorescence of SGI could be found. Under optimal conditions, the fluorescence change ratio increased linearly with clenbuterol concentration from 50 nM to 450 nM, with a detection limit of 18.8 nM. In addition, the proposed aptasensor showed good selectivity and was successfully applied for clenbuterol quantification in pork samples with recoveries of 90.37–104.98%.
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高影响因子
FOOD CONTROL
·
2014
·
IF 2.819
Jingyue Xu,Ying Li,Jiajia Guo,Fei Shen,Yeli Luo,Chunyan Sun
A new fluorescence method for detecting clenbuterol based on gold nanoparticles (AuNPs) and rhodamine B (RB) has been developed. It has been observed that the quenching of fluorescence of RB occurs in the present of AuNPs through the fluorescence resonance energy transfer (FRET). In the presence of clenbuterol , the FRET-based fluorescence of RB and AuNPs would be gradually recovered for the reason that clenbuterol could congregate the AuNPs and inhibit the FRET process . An assay for the determination of clenbuterol has been proposed based on the modulation in FRET efficiency between RB and AuNPs. Under the optimum conditions, the fluorescence recovery efficiency of the assay is proportional to the concentration of clenbuterol. The detection limit in swine feed was calculated to be 0.008 mg/g and the recoveries were in the range of 97%–102%. In short, the proposed method provides advantages such as rapidity, simplicity, low cost and high sensitivity, making it promising for the rapid screening of clenbuterol residues.
研究领域:
分析化学
·
纳米生物技术
·
药物残留检测
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食品安全
·
分子诊断
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Qian Ran,Boyan Sun,Xiaomei Wang,Jiangkang Ren,Chujun Liu,Qiqi Wang,Zimo Liu,Jianzhong Shen,Jincheng Xiong,Haiyang Jiang
s The illicit use of clenbuterol (CLB) in livestock farming poses significant public health risks, necessitating sensitive and reliable detection methods. Conventional gold nanoparticle-based lateral flow immunoassays (AuNPs-LFIAs) are often hampered by limited sensitivity and a narrow dynamic range. Here, we developed a dual-readout nanohybrid probe via an electrostatic integration of AuNPs into metal-AIEgen frameworks (MAFs). The intrinsic fluorescence of MAFs (excitation≈350 nm, emission≈475 nm) exhibits minimal spectral overlap with AuNP absorption (≈520 nm), thereby retaining 78.6% of the MAF's quantum yield while achieving a 2-fold enhancement in colorimetric absorbance. The resulting probe integrates bright fluorescence and intense colorimetric signal into a robust LFIA strip. For CLB detection, the constructed dual-readout LFIA achieved an ultra-wide linear range (0.01–3.90 ng/mL) and a significantly improved detection limit (0.007 ng/mL), demonstrating a 41-fold wider linear range compared to conventional AuNPs-LFIA. The method demonstrated satisfactory recovery rates (87.5–108.3%) in spiked swine urine and pork samples, and showed high consistency with high-performance liquid chromatography-tandem mass spectrometry results when applied to real samples. This work presents a generalizable design principle for spectral-interference-minimal dual-readout probes, advancing LFIA technology with high sensitivity, an ultrabroad dynamic range, and reliable performance for on-site “farm-to-table” monitoring.
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高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Hongwei Tang,Ying Zhong,Xiangrong Zeng,Yu Sang,Furui Lin,Yifu Zhu,Zhongping Chen,Lanjiao Xu,Zhong Huang,Peicong Zhou
The traditional preparation method of graphene is complicated in operation and high-cost, which severely limits the further application of graphene. Hence, a new strategy for the rapid, large-scale, low-cost preparation of graphene can effectively solve the current problems. In this work, laser-induced graphene (LIG) was rapidly prepared in one step based on polyimide (PI) film as the substrate, by using laser direct writing technology, and prepare LIG into a flexible electrode. Besides that, LIG flexible electrode was modified the by chemical method, sodium borohydride was used as reducing agent to reduce chloroplatinic acid, and Pt metal nanoparticles were loaded on the surface of LIG electrode in situ to prepare Pt-LIG composite electrode material. Compared with LIG electrode, Pt-LIG electrode has high conductivity, larger surface area and better electrocatalytic ability. It is used as a new type of electrochemical sensing platform to perform electrochemical detection of clenbuterol (CLB), obtaining a wide detection linear range of 0.1 – 820.9 μM, and a lower limit of detection (LOD) of 0.072 μM. Moreover, the CLB in the actual sample of beef was detected at the same time, and an effective and acceptable recovery rate was obtained.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Sijie Liu,Rui Shu,Chen Nie,Yuechun Li,Xing Luo,Yanwei Ji,Xuechi Yin,Jing Sun,Daohong Zhang,Jianlong Wang
Although lateral flow immunoassay (LFIA) as a point-of-care (POC) diagnostic tool has been widely used because of numerous merits, it remains challenging to realize higher sensitivity, easier labeling, and fewer antibodies consumption. Herein, a bioresource-derived tannic acid (TA)-supported immuno-network based LFIA for clenbuterol (CLE) monitoring was proposed by employing poly TA nanospheres (PTAN) as the new tracer. Attributing to the effective protein-enrichment ability of TA, plenty of goat anti-mouse immunoglobulins (GAMI) were first absorbed on the surface of PTAN and then monoclonal antibodies (Ab CLE ) were added to form immuno-network. Compared with directly coupling antibodies, the utilization efficiency of Ab CLE was significantly improved. Thus, the detection sensitivity (take the cut-off value as an example) was enhanced 5-fold and 10-fold at least compared with PTAN-Ab CLE and colloidal gold-based LFIA, respectively. Additionally, the proposed method was successfully verified in beef and pork liver and provided an effective strategy for food safety monitoring.
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-9
·
IF 5.479
Yu Qiongqiong,Liu Jing,Zhao Guangying,Dou Wenchao
An immunochromatographic assay (ICA) is presented that can be applied to simultaneous detection of clenbuterol (CLE) and ractopamine (RAC). It is making use of two red and blue silica nanoparticles (SiNPs) that act as labels for encoding the antibodies. This design permits multiplexed analysis in a single test line and does not require an external source for photoexcitation. Anti-CLE was labeled with red SiNPs, and anti-RAC with blue SiNPs. The capture antigens CLE-BSA and RAC-BSA were placed onto the conjugate pad and the test line of the test strip, respectively. Under bare eye examination, no cross-colored lines or nonspecific bioconjugate adsorption were observed, and the visible limit of detections for CLE (red) and RAC (blue) are 3 and 2 ng‧mL −1 , respectively. This design allows for multiplexed detection with reduced device dimensions and costs, and with easy integration and manufacturing. Conceivably, the method may be extended to simultaneous determination of numerous other analytes.
The principle of qualitative detection strategy of multiplex immunochromatographic assay for clenbuterol (CLE) and ractopamine (RAC) is schematically illustrated. Depending on the type and ratio of organic dyes, the color of colored silica nanoparticle can be tuned from red to purple and even to black (lower right corner).
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 34
·7792-7800
·
IF 5.344
Rui Lv,Enhui Wu,Renfei Wu,Wen Shen,Chongqing Ma,Rui Shi,Ruochen Guo,Mingwang Shao,Jian Liu
There is increasing demand for anti-doping drug monitoring in sports and food safety checks by developing sensitive and fast analytical methods. Here we report the development of hybrid Ir/SiNW as a new MALDI matrix for the detection of small molecules. This matrix is characterized by sufficient UV absorption, low-noise background, and high efficiency in ionization of small molecules. Sensitive detection of clenbuterol (LOD: 0.18 pmol) and a variety of other small molecules has been achieved using the Ir/SiNW matrix with a reproducible performance. Compared to the individual components separately, the matrix of hybrid Ir/SiNW synthesized via in situ growth can promote the MS signal intensity by up to 10 fold under identical experimental conditions. We provide a unique mechanism for the high performance of the hybrid Ir/SiNW matrix with the characteristic properties of hydrogen atom transfer and enhanced protonation at the interface of the hybrid nanostructures. Our approach of using a hybrid Ir/SiNW matrix enables detection of clenbuterol quantitatively in complicated biological samples and in vivo experiments, promising a useful tool for food security and anti-doping drug monitoring in sports.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Nuo Duan,Wenhui Gong,Shijia Wu,Zhouping Wang
Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA library immobilized systematic evolution of ligands by exponential enrichment (SELEX) technique. After cloning and sequencing, five aptamer candidates were picked out for affinity and specificity assays based on a graphene oxide (GO) adsorption method. The results showed that the aptamer CLB-2 binds specifically against CLB with a dissociation constant, Kd, value of 76.61 ± 12.70 nM. In addition, an aptamer-based fluorescence bioassay was established for CLB analysis. The correlation between the CLB concentration and fluorescent signal was found to be linear within the range of 0.10 to 50 ng/mL with a limit of detection of 0.07 ng/mL. It has been further applied for the determination of CLB in pork samples, showing its great potential for sensitive analysis in food safety control.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Conghui Jia,Shaochi Wang,Sijie Liu,Yuechun Li,Xianghong Xie,Huilan Hu,Jing Ren,Yanli Tian,Jing Sun,Daohong Zhang,Mingqiang Zhu,Jianlong Wang,Yining He
Immunochromatographic test strips (ITSs) have gained great practicability in point-of-care testing (POCT). Developing novel labels of ITSs with an additional signal to carry out quantitative analysis favors the development of sensitive POCT. Herein, tannic acid (TA) assisted stripping WS 2 nanosheets (TA-WS 2 NSs) based on colorimetric/photothermal ITS (TWITS) for clenbuterol (CLE) monitoring. After WS 2 NSs loaded TA, it retained excellent photothermal performance and an intact surface structure for monoclonal antibody (mAb) labeling, at the same time, the stability of WS 2 NSs and the activity of mAb were improved effectively. Satisfactory, the visual limit of detection (vLOD) of the photothermal signal was 0.3 ng/mL, which was enhanced 7-fold at least compared with traditional gold-based nanoparticles ITS (GNPs ITS). Importantly, this work can offer a new referenced method for other small molecule pollutants.
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高影响因子
Polymers
·
2019
·Vol. 11
·期 10
·1635
·
IF 3.164
Xiangyun Zhao,Yuliang Mai,Dongchu Chen,Min Zhang,Huawen Hu
The noxious clenbuterol misapplied as the feed additive has posed an enormous threat to humans who actively rely on the food chains with high potential of contamination by clenbuterol, such as pork and beef. It is, therefore, highly desirable to develop novel materials and strategies for dealing with the clenbuterol. Herein, functional polymer microspheres prepared by Pickering emulsion polymerization were explored for the selective enrichment of the clenbuterol, and their structure and oxygen functionalities could be tailor-made by a molecular imprinting process. The clenbuterol imprinting was adequately demonstrated to not only increase the particle size (~52 nm vs. ~42 nm) and create cavities for the accommodation of the clenbuterol molecules, but also reduce the oxygen functionalities of the resulting molecularly imprinted polymer microspheres (MIPMs) by approximately 4 at.%, which is believed to correlate with the high specificity of the MIPMs. Various characterization methods were employed to evidence these findings, including scanning electron microscopy, BET measurements, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and elemental mapping examination. More importantly, the MIPMs showed a markedly superior enrichment capability towards clenbuterol to the counterpart, that is, non-molecularly imprinted polymer microspheres (NIPMs). Compared to the NIPMs without specificity for clenbuterol, the MIPMs exhibited an impressive selectivity to clenbuterol, with the relative selectivity coefficient (k′) values largely exceeding 1, thus corroborating that the useful molecular imprinting led to the generation of the binding sites complementary to the clenbuterol molecule in the size and functionalities. The MIPMs were also employed as the stationary phase to fabricate molecularly imprinting solid-phase extraction column, and the spike recovery was demonstrated to be not significantly decreased even after nine cycles. Furthermore, the reliability of the method was also evidenced through the comparison of the MIPMs prepared from different batches.Keywords:molecularly imprinted polymers;polymer microspheres;Pickering emulsion;oxygen functionalities;adsorption;selectivity
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-12
·
IF 5.479
Ge Yu,Qu Mingren,Xu Lanjiao,Wang Xiaoqiang,Xin Junping,Liao Xiaoning,Li Meifa,Li Mingfang,Wen Yangping
A series of phosphorene (BP) nanocomposites was prepared to realize simultaneous electrochemical determination of clenbuterol (CLB) and ractopamine (RAC). CLB and RAC are the most commonly used β-agonists in animal-derived food. The BP nanohybrid was obtained by co-decoration with both mono(6-mercapto-6-deoxy)-β-cyclodextrin and poly(3,4-ethylenedioxythiophene) nanoparticles. It displays high stability, antifouling capability, a large electrochemical active surface and good electrochemical response. The electrochemical assisted antifouling strategy was selected by further eliminating the fouling of the electrode surface using continuous cyclic voltammetry. The electrode was employed for electrochemical sensing of CLB and RAC at typical peak voltages of 0.8 and 1.0 V (vs. SCE). Responses are linear in the 0.3–90 μM concentration range for CLB, and from 0.3 to 9.4 μM for RAC under optimal conditions. The limit of detection are 0.14 and 0.12 μM, respectively. The sensor was employed for simultaneous determination of CLB and RAC in (spiked) beef, feed and bovine serum samples with acceptable recoveries.
An electrochemically assisted anti-fouling method for simultaneous voltammetric nanosensing of clenbuterol (CLB) and ractopamine (RAC) in edible cattle product samples using high-stable and anti-foul phosphorene (BP) co-decorated with mono(6-mercapto-6-deoxy)-β-cyclodextrin (S-β-CD) and poly(3,4-ethylenedioxythiophene) (PEDOTNPs).
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 18
·10407-10414
·
IF 3.269
Meng Yu,Yongjun Hu,Jianzhi Liu
In recent years, the illegal abuse of β2-adrenergic agonists in livestock for growth promotion has been reported frequently. For conventional immunoassay methods, the development of quantitative and simultaneous detection is a great challenge. In this report, a highly sensitive multiplexed immunoassay based on competitive surface-enhanced Raman scattering (SERS) spectroscopy has been developed for the simultaneous detection of clenbuterol and ractopamine. The SERS nanoprobes based on gold nanoparticles (AuNPs) were attached by 4,4′-dipyridyl (DP), clenbuterol antibody, and 2,2′-dipyridyl (BP), ractopamine antibody respectively. The mode of detection of multiple antibodies was implemented by competitive binding between free clenbuterol, free ractopamine and clenbuterol–BSA, ractopamine–BSA immobilized on the substrates with their antibodies labeled on SERS nanoprobes. The results indicated that compared to conventional methods, the proposed method can detect clenbuterol and ractopamine simultaneously over a wide concentration range (1–1000 pg mL−1) with a lower limit of ca. 1.0 pg mL−1, which holds great potential for applications in biochemical analysis and food safety.
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高影响因子
危险品化学品经营许可证（带存储）