ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Yonghai Song,Hongyu Liu,Hongliang Tan,Fugang Xu,Jianbo Jia,Lixue Zhang,Zhuang Li,Li Wang
A facile and effective electrochemical sensing platform for the detection of glucose and urea in one sample without separation was developed using chitosan-reduced graphene oxide (CS-rGO)/concanavalin A (Con A) as a sensing layer. The CS-rGO/Con A with pH-dependent surface net charges exhibited pH-switchable response to negatively charged Fe(CN)63–. The principle for glucose and urea detection was essentially based on in situ pH-switchable enzyme-catalyzed reaction in which the oxidation of glucose catalyzed by glucose oxidase or the hydrolyzation of urea catalyzed by urease resulted in a pH change of electrolyte solution to give different electrochemical responses toward Fe(CN)63–. It was verified by cyclic voltammograms, differential pulse voltammograms, and electrochemical impedance spectroscopy. The resistance to charge transfer or amperometric current changed proportionally toward glucose concentration from 1.0 to 10.0 mM and urea concentration from 1.0 to 7.0 mM. On the basis of human serum experiments, the sensing platform was proved to be suitable for simultaneous assay of glucose and urea in a practical biosystem. This work not only gives a way to detect glucose and urea in one sample without separation but also provides a potential strategy for the detection of nonelectroactive species based on the enzyme-catalyzed reaction and pH-switchable biosensor.
研究领域:
电化学
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生物传感器
·
分析化学
·
酶基检测
·
生物医学传感
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Shuang Zhao,Cong Shi,Hongyang Hu,Zhengping Li,Gang Xiao,Qiaochun Yang,Peng Sun,Linyang Cheng,Wencheng Niu,Jinshun Bi,Zhao Yue
In this paper, a portable real-time sensing device was built for Concanavalin A (Con A) and glucose detection using a smartphone. The ion-sensitive field-effect transistor (ISFET) functioning at a low working point was selected as a small-size, low-power transducer, and dextran-capped silver nanoparticles (Dex-AgNPs) served as sensitive nanoprobes on the ISFET gate. Using the affinity between Con A and carbohydrates, Con A can be captured, and thus directly detected by the ISFET/Dex-AgNPs unit; then glucose can be determined indirectly by removing Con A from the ISFET/Dex-AgNPs/Con A unit via competition with dextran . The mechanism of this competition does less harm to the sensor, allows the reusability of the sensing device, and overcomes the Debye screening of the FET device in saline solutions . Powered by a button cell, the handheld device attains excellent Con A (0.16 ng mL −1 ) and glucose (10 nM) detection limit, and can practically be used for at least 20 days.
研究领域:
生物传感器
·
纳米技术
·
即时检测
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jinming Zhang,Jihang Zhang,Jiale Chen,Xiao Zhang,Jinglan Wu,Pengpeng Yang,Fengxia Zou,Hanjie Ying,Wei Zhuang
Enzyme immobilization techniques are crucial for enhancing enzyme stability and catalytic efficiency. Traditional methods such as physical adsorption and simple covalent binding often fail to maintain enzyme activity and stability. In this study, an innovative multi-level immobilization strategy was proposed to achieve efficient targeted immobilization of nuclease P1 (NP1) by fine-tuning the surface microenvironment. Molecular simulation results revealed that the distinctive electrostatic distribution and the specific placement of basic amino acids, such as lysine, on the NP1 surface caused dopamine to preferentially adsorb on areas away from NP1’s active site. This selective adsorption facilitated the directed immobilization of NP1, while the positively charged environment generated by the co-deposited surface further enhanced NP1’s adsorption capacity. This multilevel modification was found to significantly optimize the physicochemical environment of the immobilized surface through surface characterization and enzymatic testing. This strategy greatly improves enzyme activity (3590.0 U/mg), stability, and reusability (70 % after 10 cycles). In particular, NP1 on this surface exhibited an optimal Michaelis constant (K m ) of 34.0 mM and a maximum reaction rate of 5.5 mM min −1 , demonstrating the remarkable effect of the modification strategy in enhancing the enzyme catalytic performance. The present study provides an efficient and stable immobilization platform for enzyme catalytic applications by precisely modulating the surface microenvironment and the oriented immobilization strategy, which has an important potential for practical applications. This stable and reusable NP1 platform allows for efficient DNA/RNA cleavage, facilitating its application in industrial biocatalysis, biomedical enzyme-based processes, and biosensors.
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Jing Zheng,Jianping Wang,Dandan Song,Jingli Xu,Min Zhang
An ultrasensitive electrochemical sensor was developed for the quantitative determination of carcinoembryonic antigen (CEA), which is often utilized as a tumor marker and a prognostic indicator. In the paper, first, copper silicate integrated with nitrogen-doped magnetic carbon microtubes (NCMTs@Fe3O4@Cusilicate) was synthesized and employed to enrich concanavalin A (ConA), which can be used as an affinity probe to selectively capture CEA. Through chemical chelation between a copper ion and Con A, the combination of Cusilicate/ConA was greatly improved. Furthermore, gold nanoclusters (AuNCs) immobilized with aptamers were provided for signal amplification. Thus, in the presence of the target protein, CEA, in a sandwich manner, was formed, and AuNCs were gathered on the electrode through a magnetic field. Electrochemical oxidation of AuNCs to AuCl4– was performed, and immediately differential pulse voltammetry was performed, resulting in an electrochemical response because of the reduction of AuCl4–. On the basis of these merits, the constructed sensing system provided a ultrasensitive detection scheme for CEA with a linear range from 0.03 to 6.00 ng·mL–1 and a low detection limit of 5.38 pg·mL–1. In addition, the proposed strategy was employed to detect CEA in serum, which showed excellent correlations with standard immunoassay. The excellent performance indicated that the proposed sensor has significant potential for clinical diagnosis.
研究领域:
生物医学工程
·
肿瘤标志物检测
·
纳米技术
✓ 已验证
DOI
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Luoyuan Shi,Xinying Jiang,Shuang Sun,Chunping Jia,Xiaojun Bian,Jianlong Zhao
Rapid and reliable detection of foodborne pathogens is essential for ensuring food safety, yet achieving high selectivity and sensitivity in complex matrices remains challenging. Herein, we report a sensitive and selective electrochemical sensing platform integrating a bacteria-imprinted polymer (BIP) capture interface with concanavalin A-functionalized Fe 3 O 4 (Fe 3 O 4 -ConA) for the detection of Escherichia coli O157:H7 ( E. coli O157:H7). The BIP film was electropolymerized in situ on glassy carbon electrode to generate recognition sites for efficient capture of E. coli O157:H7. Subsequently, Fe 3 O 4 -ConA bound to surface glycans on the captured bacteria through lectin-glycan interactions, forming a sandwich-like sensing interface. The anchored Fe 3 O 4 nanozyme catalyzed the H 2 O 2 -mediated oxidation of o -phenylenediamine, producing amplified electrochemical signals for bacterial quantification. Benefiting from the combination of imprinting-based recognition and catalytic amplification, the sensor achieved an ultrasensitive detection limit of 2.8 CFU mL −1 and a linear range from 10 1 to 10 5 CFU mL −1 . Recoveries of 93.29–113.62% were obtained in spiked milk, fish, and juice samples, with reliable detection down to 10 CFU mL −1 in complex food matrices. This work provides an effective strategy for constructing nanozyme-amplified electrochemical sensors for sensitive and selective monitoring of foodborne pathogens.
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 19
·14503
·
IF 5.6
Zhiye Zhao,Ye Li,Shancheng Guo,Yuxu Chen,Haiaolong Yin,Yaxian Li,Guiguang Cheng,Lei Tian
Acute hepatitis (AH) is a common liver disease with an increasing number of patients each year, requiring the development of new treatments. Hence, our work aimed to evaluate the therapeutic effect ofOryza sativaL. indica (purple rice) seed coat on concanavalin A (ConA)-induced AH and further reveal its potential mechanisms. Purple rice seed coat extract (PRE) was extracted with hydrochloric acid ethanol and analyzed through a widely targeted components method. We evaluated the effects of PRE on AH through histopathological examination, liver function, gut microbiota composition, and the intestinal barrier. The potential targets of PRE on AH were predicted by bioinformatics. Western blotting, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) staining, and corresponding kits were used to investigate PRE effects on predicting targets and associated signaling pathways in AH mice. In AH model mice, PRE treatment increased transformed mouse 3T3 cell double minute 2 (MDM2) expression to inhibit apoptosis; it also markedly downregulated protein kinase C alpha (PKCα), prostaglandin-endoperoxide synthase 1 (PTGS1), and mitogen-activated protein kinase 1 (MAPK1) activity to alleviate inflammation. Thus, PRE treatment also recovered the intestinal barrier, decreased the lipopolysaccharide (LPS) levels of plasma and the liver, enhanced liver function, and improved the composition of intestinal microbiota. In general, PRE targeting MDM2, PKCα, MAPK1, and PTGS1 ameliorated ConA-induced AH by attenuating inflammation and apoptosis, restoring the intestinal barrier, enhancing the liver function, and improving the gut microbiota, which revealed that the purple rice seed coat might hold possibilities as a therapeutic option for AH.Keywords:Oryza sativaL. indica;acute hepatitis;MDM2;PKCα;PTGS1;gut microbiota
研究领域:
药理学
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肝病学
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免疫学
·
生物信息学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Xiaojian Li,Yaoguang Wang,Li Shi,Hongmin Ma,Yong Zhang,Bin Du,Dan Wu,Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
研究领域:
生物传感
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Wen-Bo Zhao,Xiao Liang,Zi-Tao Zhong,Ji-Tao Li,Dan Mei,Juan Yang,Qin-Qin Han,Ya-Ling Yang
Salmonella ( Sal ) and Escherichia coli ( Esc ) are two common foodborne pathogens that cause significant harm to humans. Therefore, strict control of Esc and Sal in food and the environment is essential. Herein, we developed a colorimetric/fluorescence dual-mode strategy for the detection of two pathogenic bacteria. Taking advantage of the characteristic that concanavalin A (ConA) can capture bacteria via the recognition of the sugar residues, it was coupled with magnetic nanoparticles (MNPs) to form MNPs@ConA as the capture probe. In addition, the antibody (Ab) can specifically recognize the bacteria, and after coupling with CdTe quantum dots (QDs), QDs@Ab was employed as the signal probe. This study shows that MNPs can catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxTMB to be recognized by the naked eye, while QDs exhibited strong fluorescence, which can also be observed by the naked eye, allowing for a clear differentiation in Sal and Esc detection. The signal was linearly correlated with the target bacteria with a satisfied visualized results. The practicability of the method was verified using milk tea, milk, and juice samples, with recovery rates ranging from 93.5 % to 106 %. The feasibility of this method for the detection of Sal and Esc was validated, and it poses a significant potential for food safety monitoring.
研究领域:
生物传感器
·
食品安全
·
纳米技术
·
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Qiuyue Sha,Ruixue Guan,Huiying Su,Liang Zhang,Bi-Feng Liu,Zhaoyu Hu,Xin Liu
Protein-encapsulated gold nanoclusters (Au NCs) have recently gained much attention in biosensing and bioimaging applications owing to their remarkable fluorescence properties, nontoxicity and good biocompatibility. In this work, the mannose was grafted onto the bovine serum albumin (BSA) encapsulated Au NCs (BSA-Au NCs) to produce a mannose functionalized BSA-Au NCs (Man-BSA-Au NCs) as a new fluorescence probe for Concanavalin A (Con A) detection and human breast cancer cell imaging. A new strategy with mannose-BSA conjugates as template was firstly applied for the synthesis of Man-BSA-Au NCs, leading to a high loading of mannose (767.6 ± 7.2 mg/L) onto BSA-Au NCs. The as-prepared Man-BSA-Au NCs showed advantages of facile preparation, good monodispersity and strong red-emission. Notably, aggregation-induced fluorescence quenching of Man-BSA-Au NCs was triggered by Con A due to the multivalent cooperative interactions between mannose and Con A, which was subsequently confirmed by MALDI-TOF MS. Hence highly selective and sensitive fluorescence detection of Con A was achieved by using Man-BSA-Au NCs as a fluorescence sensor. A good linear relationship was obtained over the range of 0.01–1 μM (R 2 = 0.994) with a detection limit of 0.62 nM (S/N = 3). The developed sensor was then applied to determine Con A in human serum with acceptable recoveries of 93.70–104.8%. Moreover, based on the specific recognition between mannose and overexpressed mannose receptors on human breast cancer cells, the Man-BSA-Au NCs were successfully utilized for cancer cell imaging with good specificity.
研究领域:
纳米技术
·
生物化学
·
生物医学工程
·
癌症研究
·
分析化学
·
荧光光谱
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-11
·
IF 6.232
Tang Tingting,Yang Fan,Wang Lina,Zhao Chunxin,Nie Fei,GuopingYang
An ultrasensitive sandwich electrochemiluminescence (ECL) biosensor was designed for determination of concanavalin A (ConA) through the specific carbohydrate-ConA interactions. Three-dimensional porous metal-organic framework (Zn-MOF) was synthesized, which loaded a large amount of luminescent reagents as luminol by encapsulating into its pores to form Zn-MOF@luminol complex. Interestingly, Zn-MOF also acted as the coreactant accelerator in the luminol-H 2 O 2 ECL system. This Zn-MOF@luminol complex was used as the signal probe to achieve a super strong and stable ECL signal. In addition, three-dimensional hierarchical molybdenum disulfide nanoflower and multiwalled carbon nanotubes complex (MoS 2 NF@MWCNTs) with peroxidase-mimicking enzyme property were used as a substrate to modify the glassy carbon electrode to further enhance the ECL signal of luminol by promoting decomposition of H 2 O 2 into reactive oxygen species (ROSs). In addition to the horseradish peroxidase (HRP) catalysis effect on the luminol ECL signal, a triple amplified ConA sandwich ECL sensor with high sensitivity sensor was constructed. The linear range for ConA detection was from 0.5 pg/mL to 100 ng/mL with a detection limit of 0.3 pg/mL ( S / N = 3). The recovery test for ConA in human serum samples was performed with satisfactory results.
Graphical abstract
研究领域:
生物传感器
·
分析化学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
·
2016
·Vol. 120
·期 6
·560-570
·
IF 3.097
Hongjun Zhang,Baoling Ju,Xiaoli Zhang,Yanfei Zhu,Ying Nie,Yuanhong Xu,Qiuxia Lei
Magnolol is a pharmacological biphenolic compound extracted from Chinese herb Magnolia officinalis , which displays anti-inflammatory and antioxidant effects. This study was aimed at exploring the potential effect of magnolol on immune-related liver fibrosis. Herein, BALB/c mice were injected with concanavalin A (ConA, 8 mg/kg/week) up to 6 weeks to establish hepatic fibrosis, and magnolol (10, 20, 30 mg/kg/day) was given to these mice orally throughout the whole experiment. We found that magnolol preserved liver function and attenuated liver fibrotic injury in vivo . In response to ConA stimulation, the CD4 + T cells preferred to polarizing towards CD4 + T helper 17 (Th17) cells in liver. Magnolol was observed to inhibit Th17 cell differentiation in ConA-treated liver in addition to suppressing interleukin (IL)-17A generation. Hepatic stellate cells were activated in fibrotic liver as demonstrated by increased alpha smooth muscle actin (α-SMA) and desmin. More transforming growth factor (TGF)-β1 and activin A were secreted into the serum. Magnolol suppressed this abnormal HSC activation. Furthermore, the phosphorylation of Smad3 in its linker area (Thr179, Ser 204/208/213) was inhibited by magnolol. In vitro , the recombinant IL-17A plus TGF-β1 or activin A induced activation of human LX2 HSCs and promoted their collagen production. Smad3/Smad4 signalling pathway was activated in LX2 cells exposed to the fibrotic stimuli, as illustrated by the up-regulated phospho-Smad3 and the enhanced interaction between Smad3 and Smad4. These alterations were suppressed by magnolol. Collectively, our study reveals a novel antifibrotic effect of magnolol on Th17 cell-mediated fibrosis.
研究领域:
药理学
·
免疫学
·
肝病学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Yu Fan,Xingrong Tan,Xiaofang Liu,Xin Ou,Shihong Chen,Shaping Wei
A non-enzymatic electrochemiluminescence (ECL) sensor based on the competitive reaction between glucose and phenoxy dextran (DexP) for concanavalin A (ConA) binding sites was constructed for detecting glucose. The hybrid of graphitic carbon nitride nanosheet and 3,4,9,10-perylenetetracarboxylic acid (g-C 3 N 4 -PTCA) as signal probe was modified onto the glass carbon electrode (GCE) for immobilizing DexP through π-π interaction. Then, ConA was incubated onto the electrode via the specific binding between ConA and DexP. When the modified electrode was immersed into glucose solution, glucose would compete with DexP for ConA. With the increase in the concentration of glucose, the more ConA was taken away from the electrode, resulting in a gradual increase in ECL intensity, thus, achieving the determination of glucose. The linear range was 1.0×10 −10 ∼5.2×10 −5 M and the detection limit was 4.0×10 −11 M (S/N = 3). Moreover, this sensor possessed high sensitivity, good stability and reproducibility, indicating a promising development of non-enzymatic ECL glucose sensor.
研究领域:
分析化学
·
生物传感器
·
电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）