LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Lihan Wang,Xinyi Pang,Jiayuan Zhao,Haonan Jin,Xinyan Yang,Shiqian Fu,Shasha Cheng,Hongxuan Li,Chao Miao,Chaoxin Man,Yujun Jiang
Cronobacter sakazakii ( C. sakazakii ) is a food-borne pathogen that can be spread by powdered infant formula (PIF) to cause illness in infants. Bacteriophages (phages) are an effective potential strategy to control food-borne pathogens. Phage JK004 which isolated from the native environment of C. sakazakii was a novel virulent phage with short latent period and large burst size. JK004 had tolerance to heat, pH and osmotic-stress. The phage JK004 did not contain known virulence, transduction or antibiotic resistance genes, indicating that JK004 is safe in the practical application. In addition, because of the biofilms formation, C. sakazakii could adhere to material surfaces and was difficult to remove, resulting in persistent contamination of the PIF. JK004 could effectively remove the biofilms that have formed on sterile 96-well plates with 87% inhibition rate. While the inhibition rate of bacteria adhered on the material surfaces such as polyethylene, rubber and stainless steel was 99.95%, 99.83%, 99.84%, respectively, with significant difference between the JK004 treated group and the control group ( p < 0.05). The antibacterial activity of JK004 against C. sakazakii in PIF was also excellent. This study provides a basis for the potential application of JK004 for controlling of C. sakazakii in food production.
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DOI
高影响因子
Food Bioscience
·
2024
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IF 4.8
Danliangmin Song,Jingqi Cheng,Kai Dong,Shiyu Liu,Chaoxin Man,Shiqian Fu,Xinyan Yang,Qianyu Zhao,Yujun Jiang
Cronobacter sakazakii is a facultative anaerobic gram-negative pathogen that has attracted widespread attention for causing necrotizing enterocolitis in infants (premature and low-weight newborns) by contaminating powdered infant formula. Several studies have found that the antibiotic tolerance of Cronobacter sakazakii isolated from food sources is increasing, thereby markedly increasing the difficulty of clinical treatment of Cronobacter sakazakii infection. At present, most studies are focused on clarifying the pathogenicity of Cronobacter sakazakii , but there is still a gap in the study of necrotizing enterocolitis in neonatal rats caused by Cronobacter sakazakii with different antibiotic tolerance. To fill this gap, we analyzed and compared the ability of Cronobacter sakazakii induced by different classes of antibiotics to induce necrotizing enterocolitis in neonatal rats by mediating TLR4-NLRP3-IL-1β pathway. In the results, Cronobacter sakazakii acquired tolerance to aminoglycosides, quinolones and cephalosporins, and caused more inflammatory damage in neonatal rats, improved the ability of mediating TLR4-NLRP3-IL-1β pathway to induce necrotizing enterocolitis. Compared with the XS001 group, the NEC scores of neonatal rats in XS001-Amp, XS001-Ami, XS001-Ofl and XS001-Ceo groups were increased by 8.3%, 58.3%, 50.0% and 58.3%. The acquisition of penicillin and tetracycline tolerance had little effect on the ability of Cronobacter sakazakii to induce necrotizing enterocolitis in neonatal rats. The ability of Cronobacter sakazakii to induce necrotizing enterocolitis in neonatal rats was significantly different due to the type and tolerance of antibiotics.
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
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IF 8
Qing Kang,Shuai-qi Zhang,Tong Lin,Jin-zhao Li,Chen-jing Ma,Jing-bo Jiao,Cheng Li,Xin-jun Du,Shuo Wang
Cronobacter sakazakii ( C. sakazakii ), a foodborne pathogen frequently present in contaminated powdered infant formula (PIF), poses a substantial threat to food safety. Herein, a novel fluorescent biosensor based on gold nanoclusters (AuNCs) that exhibit aggregation-induced emission (AIE) when triggered by nucleic acids was developed. This biosensor integrates the exponential amplification reaction (EXPAR) with hybridization chain reaction (HCR) signal amplification (EXHCR), enabling ultrasensitive detection of C. sakazakii . The aggregations of glutathione protected AuNCs assembled by G-rich sequences (HP1, and HP2) was affected by the EXHCR reaction initiated with C. sakazakii , forming G-quadruplex nanowires, leading to the dis-aggregation of AuNCs and a turn-off response of fluorescence, thereby realizing the detection of C. sakazakii . The changes are discernible on cellulose paper with the naked eyes. The biosensor demonstrates exceptional selectivity due to the integration of an aptamer and a dual signal amplification strategy. Under optimized conditions, it achieves a remarkable detection limit of 1.10×10 0 CFU/mL, and a broad linear range from 1.10×10 0 to 1.10×10 7 CFU/mL . Furthermore, the biosensor’s capability to identify C. sakazakii in artificially contaminated milk powder with recoveries ranging from 97.8% to 107%, underscores its reliability and accuracy. Overall, this study offers a promising approach for nucleic acid and AIE-based detection, exhibiting considerable potential for developing highly efficient bacterial detectors.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaodong Cao,Minli Liu,Jie Lu,Hongge Lv,Jinzhe Han,Shudong He,Yongkang Ye,Xiangyang Chen,Zhaojun Wei,Haisong Zheng
This work presented an electrochemical biosensor for the detection of virulence outer membrane protein A ( ompA ) gene of Cronobacter sakazakii ( C. sakazakii ), which was based on the mimic peroxidase activity of boron doped quantum dots-Au nanoparticles (BQDs-AuNPs) and a signal amplification strategy of exonuclease III (Exo III)-assisted target-recycling (EATR). The electrochemical signal was come from the electrochemical reduction of H 2 O 2 by BQDs-AuNPs nanozyme. Due to the enhanced peroxidase-mimic electrocatalytic efficiency of BQDs-AuNPs and the EATR strategy, the biosensor showed a broad linear range (1.0 × 10 -15 – 1.0 × 10 -8 mol L -1 ) and a low limit of detection (LOD, 4.0 × 10 -17 mol L -1 ). The constructed biosensor could also be applied in direct detection of extracted DNA from C. sakazakii . A good linear relationship (7.8 – 7.8 × 10 6 CFU mL −1 ) between the logarithm concentration of C. sakazakii and electrochemical signal was obtained with a LOD of 2.6 CFU mL −1 . The biosensor was applied in the detection of impA gene segments in contaminated infant formula with recoveries ranged in 83.4 – 108.2%.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）