Cell Reports Medicine
·
2025
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IF 10.6
Dongjie Zhang,Zhaoyang Cheng,Yali Song,Huandi Li,Lin Shi,Nan Wang,Yingwen Peng,Renan Chen,Nianzheng Sun,Min Han,Fengjiao Hu,Chuntao Zong,Rui Zhang,Si Chen,Conghui Zhu,Xiaoli Zhang,Xiaobo Li,Xiaopeng Ma,Changbei Shi,Xiaofei Zhang,Rui Liu,Ziqi Ren,Lin Wang,Qi Zeng,Tingting Zeng,Xueli Chen
Rapid identification and accurate diagnosis are critical for individuals with acute leukemia (AL). Here, we propose a combined deep learning and surface-enhanced Raman scattering (DL-SERS) classification strategy to achieve rapid and sensitive identification of AL with various subtypes and genetic abnormalities. More than 390 of cerebrospinal fluid (CSF) samples are collected as targets, encompassing healthy control, AL patients, and individuals with other diseases. Sensitive SERS detection could be achieved within 5 min, using only 0.5 μL volume of CSF. Through integrated feature fusion (1D spectra and 2D image) with a transformer model, the classification method is developed to screen and diagnose AL patients, demonstrating exceptional classification performances of accuracy, sensitivity, specificity, or reliability. Also, this approach demonstrates remarkable versatility and could be extended to the classifications of meningitis diseases. The sensitive DL-SERS classification platform has the potential to be a powerful auxiliary in vitro diagnostic tool.
研究领域:
生物医学工程
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肿瘤学
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医学人工智能
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分析化学
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分子诊断
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产品关联
DOI
PubMed
高影响因子
Matter
·
2025
·
IF 17.5
Jie Shen,Yun Wang,Min Yao,Shubo Liu,Zhiguang Guo,Li Zhang,Ben Wang
Lumbar puncture is a minimally invasive technique for delivering drugs into cerebrospinal fluid (CSF). However, its effectiveness is limited by the spine’s extensive length and the narrow, complex CSF spaces, which hinder efficient passive diffusion of therapeutic agents. Here, we have engineered a differentiable stem cell-assembled soft robot (SCASR) by using a three-dimensional self-assembly process in liquid marbles. These biologically viable robots can interface with the tissues inside the CSF, adapting to the complex surroundings while navigating. With X-ray imaging and magnetic actuation, the SCASRs can be guided toward targeted regions. The SCASRs autonomously disassemble into individual cells, which then differentiate into neural cells to facilitate the restoration of neural connections, thereby aiding the recovery of paralyzed lower limbs. The proposed strategy provides an imaging-based therapeutic system for nerve injury, enabling accessibility of hard-to-reach spinal regions and facilitating efficient therapy in minimally invasive manner, by means of soft microrobots.
研究领域:
生物医学工程
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神经科学
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干细胞研究
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机器人学
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治疗系统
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产品关联
DOI
高影响因子
Molecular Catalysis
·
2023
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IF 4.6
Tongyun Zhang,Chengping Zhang,Xiaoxun Ma,Hengdao Quan
3,3,3-trifluoropropyne (HFY-2223tz) is a novel refrigerant with great physicochemical properties. Though preparation of HFY-2223tz from halogenated olefins is theoretically effective, it remains great challenges for dehydrohalogenation from halogenated olefins, limiting the detailed knowledge. Here, we established a facile investigation to explore the gas-phase dehydrochlorination reaction of 2‑chloro-3,3,3-trifluoropropene (HCFO-1233xf) over various halides (Na, K, Rb, Cs) supported active carbon (AC) catalysts. The CsF was concluded as the optimal active component with best dehydrochlorination selectivity and conversion of HCFO-1233xf. The CO 2 -TPD results illustrated that dehydrochlorination of HCFO-1233xf proceeded through E1cB mechanism. Mechanism study revealed the evolution of side-products including ( Z / E )-1,3,3,3-tetrafluoropropylene, 2,3,3,3-tetrafluoropropylene, ( Z / E )-1‑chloro‑3,3,3-trifluoroproene, and 3,3,3-trifluoroprop-1-ene, etc. However, observable deactivation of CsF catalyst was noticed, which, according to combined XRD and XPS results and density functional theory (DFT) calculations, was attributed to the F/Cl exchange between HCFO-1233xf and catalyst, leading to rapid fluorine consumption. This work thus provides basic knowledge about heterogeneous catalytic synthesis of HFY-2223tz from dehydrohalogenation of halogenated olefins.
研究领域:
化学工程
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催化
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-25
·
IF 12.6
Han Yuhan,Gu Jiacheng,Xu Miaomiao,Ma Yufei,Weng Weiji,Feng Qiyuan,He Zhenghui,Qi Wenlan,Mao Qing,Jiang Jiyao,Feng Junfeng
Microglia play a critical role in neuroinflammation, a key secondary injury mechanism following traumatic brain injury (TBI). The colony-stimulating factor 1 receptor (CSF-1R) inhibitor PLX5622 has shown promise in suppressing neuroinflammation by depleting microglia, but it lacks specificity in targeting microglia at the injury site. To overcome this limitation, we developed PLX5622 nanoparticles functionalized with the CAQK peptide for lesion-specific targeting and combined them with a hydrogel (GelMA-PPS) that possesses potent reactive oxygen species (ROS) scavenging capabilities. This nanoparticle-hydrogel drug delivery system (GelMA-PPS/P) significantly enhanced the delivery efficiency and therapeutic efficacy of PLX5622 in TBI treatment. Localized administration of this system effectively depleted microglia at the injury site, suppressed neuroinflammation, and reduced the release of inflammatory cytokines. Its ROS scavenging ability was also validated in vitro and in vivo. Together, these effects synergistically improved neurological function recovery in TBI mouse models. This innovative strategy offers a comprehensive and targeted approach to managing neuroinflammation after TBI, providing a promising avenue for advancing TBI therapies.
研究领域:
神经科学
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生物医学工程
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药剂学
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炎症研究
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纳米技术
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DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2023
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IF 4.9
Haifeng Ni,Sunxiang Qian,Jie Lu,Jie Feng,Xiao-zhou Mou,Jing Zhang
Chemoimmunotherapy is an effective cancer treatment method. Drugs are always combined and used in treating cancer. However, the characteristic of drugs varies, making it challenging to control their release kinetics utilizing delivery devices with a single microstructure. In this study, we attempted to uniformly size drugs of varying molecular weights and confine them in a compartment where immune cells may be recruited and moved freely. Dextran microgels were created as modular drug libraries to address the cryogel burst release of small molecule drugs. Then, modular drug libraries and granulocyte-macrophage colony-stimulating factor (GM-CSF) were integrated into cryogels for a combined treatment. Herein, alginate was zwitterion modified to avoid the immune reaction generated by the material. Because of its macroporous structure, the cryogel could be injected into the body, eliminating invasive surgical procedures. Results demonstrated that multiscale delivery platforms could improve the synergistic effect of various medications on tumor treatment.
研究领域:
药学
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生物医学工程
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癌症治疗
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材料科学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Zhang Nan,Gao Xiaochen,Zhang Weijia,Xiong Junyao,Cao Xiaojian,Fu Zhen F.,Cui Min
Japanese encephalitis virus (JEV) is one of the most important members of the flavivirus family. It is a typical zoonotic pathogen that has caused substantial social and economic losses worldwide. The relation between JEV-induced immunosuppression and inflammatory responses has not been thoroughly investigated. In this study, cells infiltrating the brain tissue of JEV-infected mice were mainly identified as monocytic myeloid-derived suppressor cells (M-MDSCs), which subsequently differentiated into CD3+ macrophages. Co-culture with T cells showed that both splenic M-MDSCs and brain infiltrated M-MDSCs isolated from JEV-infected mice inhibited T cell proliferation through ARG1 and iNOS. The splenectomy model revealed that JEV-induced M-MDSCs were mainly derived from bone marrow and migrated to the spleen and central nervous system (CNS). The results of the transcriptome analysis and IRF7-deficient mice indicated that the ZBP1-IRF7 signaling pathway stimulated by JEV RNA played a central role in the induction of M-MDSCs. M-MDSCs migrated into the CNS through the chemokine CCL2/N-CCL2 derived from astrocytes and brain infiltrated M-MDSCs differentiated into CD3+ macrophages through a mechanism mediated by M-CSF, IL-6 and IFN-γ in the brain microenvironment. These findings provide evidence for the mechanism that JEV regulates the differentiation of M-MDSCs and thereby exacerbates pathogenicity, which represents a potential therapeutic target for Japanese encephalitis (JE).
研究领域:
免疫学
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神经病毒学
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病毒学
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致病机制
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiaoying Wang,Rong Chen,Juanjuan Hu,Wei Yuan
Amyloid-β oligomers (AβOs) was the core-biomarker of Alzheimer's disease (AD), and the detection of AβOs is very important for the early diagnosis of AD. However, existing tests for AβOs are majorly suffering from complex process and poor sensitivity. Thus, an adjustable AβOs electrochemiluminescence (ECL) aptasensor based on the synergistic effect of self-enhanced metal-organic gel (AgCNS) and triple-helix DNA system (THS) was successfully constructed. AgCNS was prepared by an extremely simple one-pot method and was an innovative luminophore with excellent ECL performance. The AgCNS-labeled complementary sequence ( [email protected] ) was interlaced with the unlabeled aptamer (Apt) carrying two short-arms fixed on the gold electrode (GE) to form the THS. Along with the specific-binding of AβOs and Apt, the THS was disrupted and adjusted flexibly between “on” and “off”, resulting in significant changes in the ECL signals. Thus, ECL detection of AβOs was sensitively achieved with a detection limit as low as 0.23 fM and the different forms of Aβ can be specifically distinguished. The aptasensor also exhibited satisfactory selectivity, stability and reproducibility. Moreover, when proposed method and ELISA-kit were simultaneously applied to artificial cerebrospinal fluid (A-CSF) samples, the obtained results were completely consistent, reflecting the potential clinical application value of this work.
研究领域:
生物化学
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分析化学
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神经科学
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诊断学
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DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2025
·
IF 7
Lv Yubing,Zhou Yanli,Lv Chaoran,Wang Mengyao,Sheng Nan,Lin Yuanyuan,Wang Cancan,Xu Maotian,Zhang Jing,Yan Mei
In view of the importance of quantification of beta-amyloid (Aβ), an “aptamer -target- peptide” sandwich electrochemical biosensor to assay Aβ42 was fabricated by using gold–palladium bimetallic nanoparticles covalent organic framework-carbon cloth (Au–Pd@COF TTF-Thi -CC) and prion protein (PrP C ) labeled with horseradish peroxidase (HRP) nanocomposites. The concentration of Aβ42 showed a positive correlation with the peak current resulting from the oxidation of hydroquinone (HQ) by HRP. In contrast, the reference current signal from thionin (Thi), derived from covalent organic framework (COF), showed a negative correlation. The current response of the electrochemical biosensor was proportional to the Aβ concentrations ranging from 0.01 to 2.5 nM with a detection limit (LOD) of 2.17 fM ( S / N = 3). The sensitivity of the sandwich electrochemical biosensor could be primarily amplified based on the substrates of Au–Pd@COF TTF-Thi -CC. The AuNPs and PdNPs not only facilitated the bonding of the linker based on the Au–S bond but also improved the performance of COF TTF-Thi -CC. Secondary sensitivity amplification of the biosensor could be achieved by using PrP C -labeled HRP to catalyze hydroquinone in solution. Furthermore, the biosensor showed excellent selectivity, anti-interference performance, and stability. The Aβ concentrations in cerebrospinal fluid (CSF) were successfully evaluated using the constructed biosensor. This assay provides a potential tool for early-stage Alzheimer's disease diagnosis.
研究领域:
生物传感器
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纳米技术
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神经化学
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电化学
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分析化学
·
阿尔茨海默病研究
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产品关联
DOI
高影响因子
Nano-Micro Letters
·
2022
·Vol. 14
·期 1
·1-15
·
IF 23.655
Shen Shen,Yi Jia,Sun Zhongda,Guo Zihao,He Tianyiyi,Ma Liyun,Li Huimin,Fu Jiajia,Lee Chengkuo,Wang Zhong Lin
A wearable triboelectric nanogenerator (denoted as CSF-TENG) is designed using biodegradable and carboxymethyl chitosan-silk fibroin (CSF) film.
In vitro biodegradation of CSF film is performed through trypsin and lysozyme. 63.1% of CSF film is removed by trypsin and lysozyme after degrading for 11 days.
An intuitive writing system is designed by CSF-TENGs-based human-machine interface to promptly track writing steps, highlight the stroke in advance, and access the accuracy of letters.
研究领域:
材料科学
·
纳米技术
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生物医学工程
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人机交互
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产品关联
DOI
PubMed
高影响因子
Progress in Orthodontics
·
2024
·Vol. 25
·期 1
·1-13
·
IF 3.5
Chen Wenyuanfeng,Qu Yuan,Liu Yining,Zhang Guorui,Sharhan Hasan M.,Zhang Xinzhu,Zhang Kunwu,Cao Baocheng
Background Orthodontic pain affects the physical and mental health of patients. The spinal trigeminal subnucleus caudalis (SPVC) contributes to the transmission of pain information and serves as a relay station for integrating orofacial damage information. Recently, glial cells have been found to be crucial for both acute and maintenance phases of pain. It has also been demonstrated that rho kinase (ROCK) inhibitors can manage different pain models by inhibiting glial cell activation. Here, we hypothesized that orthodontic pain is related to glial cells in the SPVC, and Fasudil, a representative rho/rock kinase inhibitor, can relieve orthodontic pain by regulating the function of glial cells and the related inflammatory factors. In this study, we constructed a rat model of tooth movement pain and used immunofluorescence staining to evaluate the activation of microglia and astrocytes. Quantitative real-time PCR was used to detect the release of related cytokines and the expression of pain-related genes in the SPVC. Simultaneously, we investigated the effect of Fasudil on the aforementioned indicators. Results In the SPVC, the expression of c-Fos peaked on day 1 along with the expression of OX42 (related to microglial activation), CD16 (a pro-inflammatory factor), and CD206 (an anti-inflammatory factor) on day 3 after tooth movement, followed by a gradual decrease. GFAP-staining showed that the number of activated astrocytes was the highest on day 5 and that cell morphology became complex. After Fasudil treatment, the expression of these proteins showed a downward trend. The mRNA levels of pro-inflammatory factors (IL-1β and TNF-α) peaked on day 3, and the mRNA expression of the anti-inflammatory factor TGF-β was the lowest 3 days after tooth movement. Fasudil inhibited the mRNA expression of pain-related genes encoding CSF-1, t-PA, CTSS, and BDNF. Conclusion This study shows that tooth movement can cause the activation of glial cells in SPVC, and ROCK inhibitor Fasudil can inhibit the activation of glial cells and reduce the expression of the related inflammatory factors. This study presents for the first time the potential application of Fasudil in othodontic pain.
研究领域:
牙科学
·
神经科学
·
疼痛管理
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药理学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Jiulong Zhang,Xiaoyan Sun,Mengdan Xu,Xiufeng Zhao,Chunrong Yang,Kexin Li,Fan Zhao,Haiyang Hu,Mingxi Qiao,Dawei Chen,Xiuli Zhao
Circumventing immune resistance and boosting immune response is the ultimate goal of cancer immunotherapy . Herein, we reported a tumor-associated macrophage (TAM) membrane-camouflaged nanodecoy containing a self-amplifying reactive oxygen species (ROS)-sensitive prodrug nanoparticle for specifically inducing immunogenic cell death (ICD) in combination with TAM depletion. A versatile ROS-cleavable camptothecin (CPT) prodrug (DCC) was synthesized through a thioacetal linker between CPT and the ROS generator cinnamaldehyde (CA), which could self-assemble into a uniform prodrug nanoparticle to realize a positive feedback loop of “ROS-triggered CA/CPT release and CA/CPT-mediated ROS generation.” This DCC was further modified with the TAM membrane (abbreviated as DCC@M2), which could not only target both primary tumors and lung metastasis nodules through VCAM-1/α 4 β 1 integrin interaction but also absorb CSF-1 secreted by tumor cells to disturb the interaction between TAMs and cancer cells. Our nanodecoy could effectively induce ICD cascade and deplete TAMs for priming tumor-specific effector T cell infiltration for antitumor immune response activation, which represents a versatile approach for cancer immunotherapy. Statement of significance A tumor-associated macrophage (TAM) membrane-camouflaged nanodecoy containing a self-amplifying reactive oxygen species (ROS)-sensitive prodrug nanoparticle was fabricated for the first time. This ROS-cleavable camptothecin (CPT)/cinnamaldehyde (CA) prodrug (DCC) could self-assemble into a uniform nanoparticle to realize the positive feedback loop of “ROS-triggered CA/CPT release and CA/CPT-mediated ROS generation.” After TAM membrane coating, this system (DCC@M2) could not only target both primary tumors and lung metastatic nodules but also scavenge CSF-1 secreted by tumor cells for TAM depletion for sufficient chemotherapy-sensitized immunotherapy.
研究领域:
癌症免疫治疗
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纳米医学
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药学
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免疫学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
MICROBIAL PATHOGENESIS
·
2024
·
IF 3.3
Tongyu Zhang,Yawen Tian,Xiao Zhang,Wenfeng Wang,Yingkai He,Chongbo Ge,Futing Jia,Zhannan Wang,Yanlong Jiang
The H9N2 avian influenza virus (AIV) is spreading worldwide. Presence of H9N2 virus tends to increase the chances of infection with other pathogens which can lead to more serious economic losses. In a previous study, a regulated delayed lysis Salmonella vector was used to deliver a DNA vaccine named pYL233 encoding M1 protein, mosaic HA protein and chicken GM-CSF adjuvant. To further increase its efficiency, chitosan as a natural adjuvant was applied in this study. The purified plasmid pYL233 was coated with chitosan to form a DNA containing nanoparticles (named CS233) by ionic gel method and immunized by intranasal boost immunization in birds primed by oral administration with Salmonella strain. The CS233 DNA nanoparticle has a particle size of about 150 nm, with an encapsulation efficiency of 93.2 ± 0.12 % which protected the DNA plasmid from DNase I digestion and could be stable for a period of time at 37°. After intranasal boost immunization, the CS233 immunized chickens elicited higher antibody response, elevated CD4 + T cells and CD8 + T cells activation and increased T-lymphocyte proliferation, as well as increased productions of IL-4 and IFN-γ. After challenge, chickens immunized with CS233 resulted in the lowest levels of pulmonary virus titer and viral shedding as compared to the other challenge groups. The results showed that the combination of intranasal immunization with chitosan-coated DNA vaccine and oral immunization with regulatory delayed lytic Salmonella strain could enhance the immune response and able to provide protection against H9N2 challenge.
研究领域:
免疫学
·
病毒学
·
兽医学
✓ 已验证
DOI
PubMed
高影响因子
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