Small
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2024
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IF 13
Beng Ma,Ying Yu,Jiayi Li,Yunqi Zhang,Bo Sun,Anqi Ji,Kexing Song,Linlin Shi,Hao Hu,Shegan Gao,Haoyan Cheng
Self-propelled nanomotors possess strong propulsion and penetration abilities, which can increase the efficiency of cellular uptake of nanoparticles and enhance their cytotoxicity against tumor cells, opening a new path for treating major diseases. In this study, the concept of driving nanomotors by alternately stretching and contracting a temperature-sensitive polymer (TS-P) chain is proposed. The TS-Ps are successfully linked to one side of Cu 2-x Se@Au (CS@Au) nanoparticles to form a Janus structure, which is designated as Cu 2-x Se@Au-polymer (CS@Au-P) nanomotors. Under near-infrared (NIR) light irradiation, Cu 2-x Se nanoparticles generate photothermal effects that change the system temperature, triggering the alternation of the TS-P structure to generate a mechanical force that propels the motion of CS@Au-P nanomotors. The nanomotor significantly improved the cellular uptake of nanoparticles and enhanced their penetration and accumulation in tumor. Furthermore, the exceptional photothermal conversion efficiency of CS@Au-P nanomotors suggests their potential as nanomaterials for photothermal therapy (PTT). The prepared material exhibited good biocompatibility and anti-tumor effects both in vivo and in vitro, providing new research insights into the design and application of nanomotors in tumor therapy.
研究领域:
纳米技术
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癌症治疗
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Xufeng Lu,Xiaodong Chen,Chengyin Lin,Yongdong Yi,Shengsheng Zhao,Bingzi Zhu,Wenhai Deng,Xiang Wang,Zuoliang Xie,Shangrui Rao,Zhonglin Ni,Tao You,Liyi Li,Yingpeng Huang,Xiangyang Xue,Yaojun Yu,Weijian Sun,Xian Shen
The induction of cuproptosis, a recently identified form of copper-dependent immunogenic cell death, is a promising approach for antitumor therapy. However, sufficient accumulation of intracellular copper ions (Cu 2+ ) in tumor cells is essential for inducing cuproptosis. Herein, an intelligent cuproptosis-inducing nanosystem is constructed by encapsulating copper oxide (CuO) nanoparticles with the copper ionophore elesclomol (ES). After uptake by tumor cells, ES@CuO is degraded to release Cu 2+ and ES to synergistically trigger cuproptosis, thereby significantly inhibiting the tumor growth of murine B16 melanoma cells. Moreover, ES@CuO further promoted cuproptosis-mediated immune responses and reprogrammed the immunosuppressive tumor microenvironment by increasing the number of tumor-infiltrating lymphocytes and secreted inflammatory cytokines. Additionally, combining ES@CuO with programmed cell death-1 (PD-1) immunotherapy substantially increased the antitumor efficacy in murine melanoma. Overall, the findings of this study can lead to the use of a novel strategy for cuproptosis-mediated antitumor therapy, which may enhance the efficacy of immune checkpoint inhibitor therapy.
研究领域:
肿瘤学
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纳米技术
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免疫疗法
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
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IF 7.1
Xinxing Wang,Youxiao Qi,You Shen,Yue Yuan,Lindi Zhang,Chongyu Zhang,Yanhui Sun
It is a significant and challenging task to simultaneously detection of multiple heavy metal ions with convenience, sensitivity and reliability. Herein, a novel ratiometric electrochemical sensing method was established for the simultaneously detection of three main heavy metal ion pollutants (Cd 2+ , Pb 2+ and Cu 2+ ). The sensing platform was constructed by a composite of ferrocenecarboxylic acid functionalized metal-organic framework (MOF), Fc-NH 2 -UiO-66, and thermally reduced graphene oxide (trGNO), which was designated as trGNO/Fc-NH 2 -UiO-66. NH 2 -UiO-66 has porous structure and large specific surface area, which is beneficial to the adsorption and preconcentration of heavy metal ions. The introduction of trGNO and Fc improves the conductivity and electrochemical activity of the MOF material. Moreover, the signal of Fc can be used as internal reference to develop ratiometric detection, which greatly improves the reproducibility and reliability of electrochemical detection. Based on this ratiometric electrochemical sensing platform, the simultaneous, sensitive and reliable detection of Cd 2+ , Pb 2+ and Cu 2+ was realized. This work provides a new sensing platform for simultaneous detection of multiple heavy metal ions and greatly expands the application of UiO-66-type MOFs in electrochemical field.
研究领域:
分析化学
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环境科学
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材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Junkai Gao,Miaomiao Cai,Zeguang Nie,Junwei Zhang,Yan Chen
Wettability and roughness modification of membrane surface are crucial for its utilization in the separation of oil/water mixture and emulsion. However, traditional modification method for membrane had the drawbacks of high cost, complex preparation and low flux. In this study, for the first time, based on tannic acid (TA), Cu 2+ and ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), a novel, facile, and cost-effective interface engineering method for significantly enhancing the flux and separation efficiency of polyvinylidene fluoride (PVDF) microfiltration membrane for oil/water mixture and emulsion was developed via in situ extraction of metal ions. TA and Cu 2+ could form TA-Cu 2+ complex which could deposit on the surface of PVDF microfiltration membrane, and then Cu 2+ was extracted by EDTA-2Na, which not only enhanced the surface roughness and wettability of the membrane, but also reserved the porous structure of the matrix membrane. The resulted membrane possessed superhydrophilicity and underwater superoleophobicity, which showed high membrane fluxes of 8841.94 Lm −2 h −1 bar −1 and 1440 Lm −2 h −1 bar −1 for oil/water mixtures and emulsified oil respectively, and the separation efficiency of TA/EDTA-2Na/PVDF could reach 99.99%. Moreover, the membrane still exhibited excellent stability after long soaking treatment in NaCl solution for 48 h. Thus, in this study, a new strategy for membrane surface modification was developed, which had considerable promise for real application of oily wastewater treatment.
研究领域:
环境科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2018
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IF 3.466
Fen Jiang,Yu Liu,Xiahui Wang,Zongning Yin
In this work, we investigated the feasibility of employing metal ion that coordinated with amino group grafted mesoporous silica as a pH-sensitive release carrier of insulin. Mesoporous silica particles were firstly grafted with amino groups. After coordination of metal ions onto the surface of mesoporous pore, insulin was loaded onto mesoporous silica particles through coordination bonds with metal ions. Insulin-loaded mesoporous silica particle with an acid sensitivity between the pH range of physiological environment (pH 7.4) and diabetic acidosis (pH 7.0) and with an insulin encapsulation rate of 74.50% was identified by investigating the influence factors of pH response value. The morphology of particles was confirmed by different analytical methods, indicating that insulin-loaded mesoporous silica particles maintained the rod-like and orderly hexagonal phase mesoporous structure. The coordination mechanism results showed that half of the amino groups on the carrier were coordinated with Cu 2+ , and insulin was successfully coordinated with Cu 2+ without any damage on the secondary structure. The amount of insulin coordination with Cu 2+ was positively correlated with the pH of the reaction solution. The cytotoxicity result shows that SBA 15 -NH 2 -Cu 2+ -Ins was biocompatible with cells in the concentration ranging from 25 to 100 μg·mL −1 . In vivo study preformed on rats showed that the insulin-loaded particles elicited a period hypoglycemic response. Furthermore, fluorescence and UV methods revealed that insulin-loaded mesoporous silica particles had no significant effect on the secondary structure of serum albumin .
研究领域:
药学
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纳米技术
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药物递送系统
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生物相容性
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糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Rui Chen,Yongzhen Dong,Feng Hong,Xiya Zhang,Xiaohong Wang,Jia Wang,Yiping Chen
A rapid and accurate detection method is needed for the quantitation of ochratoxin A in agricultural products due to its high toxicity. A microparticle-counting immunosensor based on polydopamine nanoparticle-mediated click chemistry was established for the highly-sensitive detection of ochratoxin A. Polydopamine nanoparticles with good biocompatibility and a strong metal-chelating ability were synthesized and conjugated with the antibody. The Coupled compounds were then used as an immune carrier to change the Cu 2+ concentration via an immuno-reaction. Some of the remaining Cu 2+ ions were reduced to Cu + ions, which caused azide-polystyrene microspheres and alkyne-polystyrene microspheres to aggregate via a Cu + ion-mediated click reaction. Particle counting was used to distinguish changes in the sizes of the polystyrene microspheres from dispersed to aggregated to detect ochratoxin A. It showed a wide linear detection range of 0.5–800 ng/mL, and a detection limit of 0.2 ng/mL. This assay provides an attractive analytical tool for the accurate detection of trace targets in complex samples.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Ling Yang,Ting Huang,Shuqiang Wang,Jingpu Tang,Chenchen Lin,Xiaoping Wu
In this work, a histidine-tagged hexapeptide functionalized monolithic material is described for the efficient capillary microextraction (CME) of microcystins (MCs) from environment waters. 1-Vinylimidazole (VIM) monomer was utilized to synthesize the matrix polymer monolith by the copolymerization with divinylbenzene (DVB) crosslinker in the presence of radical initiator azobisisobutyronitrile (AIBN). The affinity sorbent was then prepared by a metal ion chelation-based multi-step approach, in which the histidine tag peptide Trp-His-Trp-Leu-Gln-Trp (WHWLQW) was functionalized on a Cu 2+ modified polymer monolith via a chelation reaction between the imidazole group of histidine and Cu 2+ . The resultant WHWLQW functionalized monolithic material had high affinity, hierarchical porosity and good mechanical stability. Especially, it exhibited favorable enrichment capacity for MCs compared to other affinity materials as previously reported. With the optimized CME protocol, MCs at ultra-trace level can be analyzed by CME coupled to high performance liquid chromatography-mass spectrometry (HPLC-MS) in different aqueous environmental matrices. The average recoveries were ranged from 89.3% to 104.5% with relative standard deviations (RSD%) of 2.1–8.4, demonstrating high extraction efficiency and repeatability. The limits of detection (LODs) of MCs were determined to be 0.28–0.45 ng L -1 . This novel biomimetic sorbent shows promising potential for the enrichment of ultra-trace pollutants in environment waters.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Minghai Dai,Xiufei Lin,Peng Hua,Simeng Wang,Xiaoliang Sun,Chengxuan Tang,Chi Zhang,Liangle Liu
Autologous or allogeneic bone tissue grafts remain the mainstay of treatment for clinical bone defects. However, the risk of infection and donor scarcity in bone grafting pose challenges to the process. Therefore, the development of excellent biomaterial grafts is of great clinical importance for the repair of bone defects. In this study, we used gas-assisted microfluidics to construct double-cross-linked hydrogel microspheres with good biological function based on the ionic cross-linking of Cu 2+ with alginate and photo-cross-linking of gelatin methacryloylamide (GelMA) by loading vascular endothelial growth factor (VEGF) and His-tagged bone morphogenetic protein-2 (BMP2) (AGMP@VEGF&BMP2). The Cu 2+ component in the microspheres showed good antibacterial and drug-release behavior, whereas VEGF and BMP2 effectively promoted angiogenesis and bone tissue repair. In in vitro and in vivo experiments, the dual cross-linked hydrogel microspheres showed good biological function and biocompatibility. These results demonstrate that AGMP@VEGF&BMP2 microspheres could be used as a bone defect graft substitute to promote effective healing of bone defects and may be applied to other tissue engineering studies.
CAS / MDL:
研究领域:
生物医学工程
·
组织工程
·
材料科学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Rongrong Luo,Jingjing Su,Wenying Zhang,Wenguang Huang,Qianqian Zhou,Pengchao Sun,Yongxing Zhao
Rheumatoid arthritis (RA) is characterized by high level of reactive oxygen species (ROS) and proinflammatory cytokines, which facilitate the activation of the inflammatory signaling such as NF-κB pathway and exacerbate the development of inflammation. Herein, we designed a nanodrug by encapsulating the NO donor S-nitrosoglutathione (GSNO) into an emulsion and coating the surface with a polydopamine (PDA) layer to yield GSNO@PDA, which simultaneously scavenged the extra ROS and suppressed NF-κB signaling for potent RA treatment. To enhance the cellular uptake and NO generation efficiency, dextran sulfate (DS) and Cu 2+ were anchored on the surface of GSNO@PDA to obtain the final formulation GSNO@PDA@DS. Our results demonstrated that GSNO@PDA@DS were successfully prepared and the modification of DS effectively boosted the cellular uptake of GSNO@PDA@DS. Moreover, GSNO@PDA@DS lowered cellular ROS and elevated intracellular NO, resulting in a decrease of M1 phenotype, inhibition of NF-κB pathway and down-regulation of proinflammatory cytokine tumor necrosis factor-α (TNF-α). Further in vivo studies confirmed that GSNO@PDA@DS significantly relieved symptoms and bone erosion by regulating the microenvironment of RA, highlighting the potential of GSNO@PDA@DS for RA therapy through ROS scavenging and NO-mediated suppression of inflammatory signaling.
研究领域:
药理学
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纳米技术
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炎症生物学
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风湿病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TETRAHEDRON
·
2023
·
IF 2.1
Jianping Zhang,Jinming Liu,Gengwen Yin,Wenlong Hou,Haiquan Zhang
Nucleophilic substitution reaction of 1,7-dibromo-perylene diimide derivatives can expend the chemical structure and photophysical properties of perylene diimide. Here, we reported firstly the synthesis and mechanism of 1-bromo-7-hydroxyl perylene diimide derivative by the 1,7-dibromo-perylene diimide derivative in electron donor solvents (such as, NMP, DMF and etc) and under visible light irradiation . The structure of 1-dibromo-7-hydroxyl perylene diimide was confirmed by HR-MS, UV–Vis absorption spectra and comparison of UV–Vis absorption spectra with product from the heating chemical reaction. Furthermore, the 1-bromo-7-hydroxyl-perylene diimide derivative showed a higher optical response to some metal ions , such as Fe 3+ , Cu 2+ and etc.
研究领域:
有机化学
·
光化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Ning Gan,Yuqing Lin,Yiren Zhang,Yulong Qiu,Jianguo Yu,Qianhong She,Tooru Ooya,Qian Lin,Hideto Matsuyama
Tunable gating ion-conducting membranes with high water permeability and precise ion selectivity remain urgently demanded in smart nanofiltration for efficient lithium extraction . Herein, inspired by the filtration function of protein channels, we report a smart and high-performance supramolecular membrane constructed via coordination between quaternary ammonium ligands ( m -phenylenediamine/polyethyleneimine) and metal ion center (Cu 2+ ). The pore architecture of the supramolecular membrane is dynamic and can be switched by applying pH stimulus, where coordinated interchain expands when exposed to acidic operating conditions and induces concomitantly reinforced electrostatic exclusion due to enhanced local charge density. The tunable gating regularity enables operando modulation of precise ionic separation in situ. The resulting membrane exhibits significantly enhanced water permeance (19.8 L m −2 h −1 ·bar −1 ) and simultaneously promoted Li + /Mg 2+ selectivity (R Mg2+ = 96.4 %), surpassing typical trade-off between permeability and selectivity at pH 3 condition. This study demonstrates the amazing capability of environmental-responsive regulation of membrane pore-channel structure, and provides inspiration for engineering advanced supramolecular membranes for lithium extraction and beyond.
研究领域:
材料科学
·
化学工程
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Lu Tian,Wenjing Zhao,Lin Li,Yaoli Tong,Guanlin Peng,Yingqi Li
We herein provide a facile and green strategy for the fabrication of red fluorescence gold nanoclusters ( [email protected] ) with low toxicity, good stability and excellent fluorescence (λ em = 650 nm, λ ex = 320 nm) by mixing aqueous tetrachloroauric acid with diluted egg white under alkaline environment using microwave-assisted method. Confocal fluorescence imaging showed that the [email protected] can enter living cells and distribute in the cytoplasm. In addition, the fluorescence of [email protected] is sensitive to the response of Cu 2+ and Hg 2+ with a detection limit of 14.48 nM and 0.81 nM, respectively. Interestingly, we found that the fluorescent signal of the quenched [email protected] (II) system could be recovered in the presence of the glutathione (GSH) in Tris-HCl medium (pH 6.5). This assay displayed a rapid response and highly selectivity toward GSH with a detection limit of 91.9 nM. More excitingly, the fluorescent signal of the [email protected] (II) system (OFF) could be activated in living tumor cells (ON) such as HepG2 cells rather than in living normal cells (HT22 cells, OFF) without any exogenous GSH. The result showed that the system can be successfully applied for tumor cells recognition, which implied its great potential application in early prevention of cancer. Furthermore, the [email protected] can also be used for patterning, staining and temperature sensing. We propose that the highly fluorescent [email protected] can be eco-friendly and potentially large-scale development for versatile applications and so merits further study.
研究领域:
纳米技术
·
生物医学工程
·
癌症研究
·
细胞生物学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）