ANALYST
·
2018
·Vol. 144
·期 2
·716-721
·
IF 3.864
Qing Juan Guo,Zi Yu Pan,Chen Men,Wen Yi Lv,Hong Yan Zou,Cheng Zhi Huang
A simple protocol for in situ growth of Cu2−xSe nanoparticles on graphene oxide hybrids (Cu2−xSe/rGO) acting as an efficient nanozyme is developed and thus a sensitive visual detection method of cancer cells is proposed. The Cu2−xSe/rGO heterogeneous nanomaterials have been proven to exhibit high peroxidase-like activity to catalyze the reaction of the peroxidase substrate in the presence of hydrogen peroxide. Herein, we used Cu2−xSe/rGO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells and a total of 63 cancer cells (MCF-7) can be distinguished by naked-eye observation. The results showed that the Cu2−xSe/rGO hybrids could be the promising nanozyme mimetics for potential applications in bioanalytical fields.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Shen Man,Dai Xianling,Ning Dongni,Xu Hanqing,Zhou Yang,Chen Gangan,Ren Zhangyin,Chen Ming,Gao Mingxuan,Bao Jing
The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, H2O2 serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu2-xSe/GO nanostructure-based nanonzymatic H2O2 electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu2-xSe/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu2-xSe nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu2-xSe NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu2-xSe/GO@Pt/SPCE sensing electrode for H2O2 (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables in-situ real-time monitoring of H2O2 released by cells. The NIR-enhanced Cu2-xSe/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (H2O2、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.
研究领域:
生物医学工程
·
分析化学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 98
·55094-55099
·
IF 3.708
Shao Qing Lie,Hong Yan Zou,Yong Chang,Cheng Zhi Huang
In this paper, negatively charged Cu2−xSe nanoparticles (NPs), stabilized by poly(styrene sulfonic acid)sodium (PSS) (PSS–Cu2−xSe NPs), were synthesized via a simple templated method, which exhibited strong localized surface plasmon resonance (LSPR) absorption in the near-infrared (NIR) region. It was found that the prepared PSS–Cu2−xSe NPs could be aggregated by lysozymes (Lys) selectively through the electrostatic interaction, since the Lys was positively charged in a neutral medium, producing a red shift of the LSPR band starting from 980 nm to 1300 nm area. With different concentrations of Lys, the LSPR band can be dynamically tuned, and the absorbance ratio of A1300/A980 has a good linear relationship with the concentration of Lys in the range of 5–100 nM, thus supplying an effective way to tune LSPR in the NIR region of semiconductors that might be applied for sensing and photothermal conversion sciences and technologies in the NIR region.
研究领域:
材料科学
·
纳米技术
·
光学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Tong Yang,Hong Yan Zou,Cheng Zhi Huang
A new heterogeneous catalytic composite composed of nonstoichiometric Cu2–xSe nanoparticles (NPs) with high copper deficiency and graphene oxide (GO) is prepared by coembedding in electrospun nanofibers of a poly(vinylpyrrolidone) (PVP) support, wherein GO in the nanofibers is converted into reduced GO (rGO) via heat treatment. The as-prepared composite Cu2–xSe/rGO/PVP nanofibers have demonstrated superior catalytic activity toward the reduction of a refractory organic compound by taking 4-nitrophenol (4-NP) as an example. In the presence of NaBH4, the Cu2–xSe/rGO/PVP nanofibers display a synergetic effect between Cu2–xSe and rGO in PVP nanofibers compared to their independent components or corresponding nanofibers. Furthermore, the Cu2–xSe/rGO/PVP nanofibers exhibit a favorable water-stable property via heat treatment to solidify the hydrophilic PVP matrix, which makes the composite display good reusability, stability in aqueous solution, and separability from a water medium. This work not only presents a direct, convenient, and effective approach to doping semiconductor nanomaterials into polymer nanofibers but also provides fundamental routes for further investigations about the synergetic effect between different materials based on the platform of electrospun nanofibers.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Qiao Cu,Chaoqun Shang,Guofu Zhou,Xin Wang
The fabrication of freestanding electrodes for Na + storage is necessary to achieve high energy density. However, the large radius of Na + results in a large volume fluctuation and sluggish reaction kinetics of active materials, particularly at a high active material content, thereby impeding electrochemical performance with undesirable cycling performance or rate capability. In this study, a freestanding electrode based on the “NiSe grafted on Cu 2-x Se” heterostructure with double-carbon protective shells (NiSe/Cu 2-x Se@C@NCNFs) was successfully constructed for Na + storage. In this microstructure, N -doped carbon nanofibers (NCNFs) serve as the stem of the twinborn NiSe/Cu 2-x Se heterostructure with a built-in electric field, where NiSe improves Na + absorption and Cu 2-x Se enhances Na + diffusion. The “graft” design enabled the freestanding NiSe/Cu 2-x Se@C@NCNFs electrode with a high active mass content of 76.1 wt% to exhibit superior electrochemical performance for Na + storage (75 mAh g −1 at 2 A g −1 ) compared to those of Cu 2-x Se@C@NCNFs (26 mAh g −1 at 2 A g −1 ) and NiSe@C@NCNFs (9 mAh g −1 at 2 A g −1 ).
研究领域:
材料科学
·
电化学
·
能量存储
✓ 已验证
DOI
高影响因子
ANALYST
·
2015
·Vol. 140
·期 12
·4121-4129
·
IF 4.107
Hong Yan Zou,Peng Fei Gao,Ming Xuan Gao,Cheng Zhi Huang
Full understanding and easy construction of specific biosensing principles is necessary for disease diagnostics and therapeutics in the hope of creating new types of biosensors. Herein, we developed a new conceptual nanobiosensing platform by coupling nanometal surface energy transfer (NSET) and photo-induced electron transfer (PET) with polydopamine-embedded Cu2−xSe nanoparticles (Cu2−xSeNPs@pDA) and DNA-conjugated fluorescent organic dyes. The new prepared Cu2−xSeNPs@pDA has intense and broad localized surface plasmon resonance (LSPR) absorption over UV to near infrared (NIR) wavelengths, with different affinities toward ssDNA versus dsDNA. It also exhibits a high multiplexed fluorescence quenching ability, and thus can act as an acceptor in the energy transfer and electron transfer interactions between Cu2−xSeNPs@pDA and fluorescent organic dyes. As a proof of concept, a new biosensing platform has been successfully developed to target biomacromolecules such as DNA and proteins, in which the NSET and PET interactions between Cu2−xSeNPs@pDA and three different DNA-conjugated fluorescent dyes have been identified using steady-state and time-resolved fluorescence. A simple mathematical model was further applied to simulate the respective contributions of the coexisting NSET and PET to the total quenching observed for each DNA-conjugated dye in this sensing system. This study highlights the importance of understanding the mechanistic details of NSET and PET coupling processes, and the disclosed coupling mechanism of NSET and PET (NSET©PET) in the systems of Cu2−xSeNPs@pDA with wide wavelength range dyes provides new opportunities for sensitive biosensing applications.
研究领域:
生物传感
·
材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Liqiang Wang,Xiaoyu Yuan,Qianyu Cai,Yutong Chen,Zhi Jia,Qiongmei Mai,Jie Liu,Yanan Liu
ABSTRACT Mitochondrial dysfunction in microglia has been implicated as a key pathogenesis of most neurodegenerative diseases including Alzheimer's disease (AD). Abnormal production of reactive oxygen species (ROS) and neuroinflammation caused by mitochondrial oxidative stress are important factors leading to neuronal death in AD. Herein, a "dual brake" strategy to synergistically halt mitochondrial dysfunction and neuroinflammation targeting mitochondria in microglia is proposed. To achieve this goal, (3-carboxypropyl) triphenyl-phosphonium bromide (TPP)-modified Cu 2-x Se nanozymes (Cu 2-x Se-TPP NPs) with dual enzyme-like activities was designed. Cu 2-x Se-TPP NPs with superoxide dismutase-mimetic (SOD) and catalase-mimetic (CAT) activities can effectively scavenge ROS in the mitochondria of microglia and relieve mitochondrial oxidative stress. In vivo studies demonstrated that Cu 2-x Se-TPP NPs can alleviate oxidative stress and promote neuroprotection in the hippocampus of AD model mice. In addition, Cu 2-x Se-TPP NPs can regulate the polarization of microglia from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype, promote A β phagocytosis and reshape the AD inflammatory microenvironment, thus effectively attenuating AD neuropathology and rescuing cognitive deficits in AD model mice. Taken together, this strategy preventing mitochondrial damage and remodeling the inflammatory microenvironment will provide a new perspective for AD therapy.
研究领域:
神经科学
·
药理学
·
纳米技术
·
生物医学工程
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
ShaSha Wang,Lingjie Sheng,Yang Lu
In the present study, we developed a nanozyme-based direct competitive immunoassay to detect walnut allergen (Jug r 4) in foods. Walnut monoclonal antibody (mAb) and Cu 2− x Se@Bi 2 MoO 6 nanocomposites were generated to form a signal probe by electrostatic adsorption. The nanocomposites had high peroxidase-like activity and could be stored at room temperature. The linear range of the optimized assay was 137.35 ± 0.14 to 3526.0 ± 4.53 μg/L with a limit of detection of 81.63 ± 0.89 μg/L. This method was validated by detecting walnut protein in bread, biscuits, and milk samples, providing recovery rates of 70.63–127.04 %. The fast and sensitive assay was suitable for the rapid detection of walnut allergen in foods.
研究领域:
食品科学
·
分析化学
·
纳米技术
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Peng Zhou,Shuo Qi,Mingyu Zhang,Liping Wang,Qizhong Huang,Zhean Su,Zhenghao Guo,Yueli Hu,Yong Wang,Shuangqiang Chen
With the porous feature, high aspect ratio and high electronic conductivity, nitrogen-doped one-dimensional carbon nanofibers can effectively shorten ionic transport pathways and enhance the intrinsic electronic conductivity of metal selenides electrodes, showing a great potential as anode material for the next-generation lithium-ion batteries (LIBs). In this work, we have synthesized nitrogen-doped carbon nanofibers encapsulated Cu 2-x Se (N-CNFs@Cu 2-x Se) with an average particle size of 20 nm, via electrospinning and in-situ selenization process, giving an excellent lithium storage capability. Specifically, such electrodes delivered a specific capacity of 1079.1 mA h g −1 at 0.1 A g −1 after 100 cycles, while 639.4 mA h g −1 at 2 A g −1 after 1000 cycles, exhibiting an ultralong cycle life. The gradually increased capacity was attributed to phase transformation of Cu 2-x Se from crystalline to amorphous state, which leads to the capacity improvement from increased capacitive behavior. The in-situ X-ray diffraction harvest at the real time state of battery demonstrated the phase transition of Cu 2-x Se with lithium and phase regeneration among cycles, verifying copper selenide has the features of the high reversibility of conversion reaction and superior thermostability. The high capacity and long cycling life of N CNFs@Cu 2-x Se make it possible for great application potentials for high-performance lithium-ion batteries.
研究领域:
材料科学
·
能源存储
·
纳米技术
·
电化学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 8
·4361-4370
·
IF 6.739
Xijian Liu,Qian Wang,Chun Li,Rujia Zou,Bo Li,Guosheng Song,Kaibing Xu,Yun Zheng,Junqing Hu
A low-toxic difunctional nanoplatform integrating both photothermal therapy and chemotherapy for killing cancer cells using Cu2−xSe@mSiO2–PEG core–shell nanoparticles is reported. Silica coating and further PEG modification improve the hydrophilicity and biocompatibility of copper selenide nanoparticles. As-prepared Cu2−xSe@mSiO2–PEG nanoparticles not only display strong near infrared (NIR) region absorption and good photothermal effect, but also exhibit excellent biocompatibility. The mesoporous silica shell is provided as the carrier for loading the anticancer drug, doxorubicin (DOX). Moreover, the release of DOX from Cu2−xSe@mSiO2–PEG core–shell nanoparticles can be triggered by pH and NIR light, resulting in a synergistic effect for killing cancer cells. Importantly, the combination of photothermal therapy and chemotherapy driven by NIR radiation with safe power density significantly improves the therapeutic efficacy, and demonstrates better therapeutic effects for cancer treatment than individual therapy.
研究领域:
纳米医学
·
肿瘤学
·
生物材料科学
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Bin Pan,Yang Jin,Yan Chen,Juan Wang,Kangnan Wang,Sunfang Chen,Mingyang Lei,Wenguo Cui,Junjie Li,Yu Qian
The widespread abuse of antibiotics has led to the emergence of drug-resistant bacterial strains, severely complicating the treatment of infectious wounds. Traditional therapies are often limited to antibacterial or anti-inflammatory approaches, making it challenging to manage both early-stage infection and later-stage tissue regeneration simultaneously. Herein, a Cu 2−x Se nanoparticles (CSNPs) spray containing mixed-valence copper ions, enabling the programmed healing of infectious wounds is developed through light-controlled cascade regulation. In the early infection stages, the copper ions released from CSNPs exert antibacterial effects via chemodynamic therapy (CDT), with their activity significantly enhanced under near-infrared (NIR) irradiation. At later stages, Cu 2+ /Cu + ions enter cells, interact with cysteine residues in KEAP1 protein, and induce conformational changes, triggering deubiquitinase activity and suppressing macrophage-driven inflammation. Findings from in vitro and in vivo studies consistently revealed that CSNPs efficiently depleted glutathione and enhanced H 2 O 2 breakdown, leading to the generation of •OH and O 2 •- under NIR exposure, which resulted in an MRSA elimination rate exceeding 99%. Mechanistically, CSNPs bind to KEAP1, removing the ubiquitin linkage from NRF2, thereby inhibiting inflammation. This CSNPs spray provides a dual synergistic effect of antimicrobial and tissue-regenerative properties, offering an innovative approach for treating antibiotic-resistant infections in wounds.
研究领域:
纳米医学
·
抗菌治疗
·
伤口愈合
·
生物医学材料
·
光疗
·
炎症调控
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Xue Li,Lin Yang,Chen Men,Yi Fen Xie,Jia Jun Liu,Hong Yan Zou,Yuan Fang Li,Lei Zhan,Cheng Zhi Huang
Photothermal effects (PTEs) have been greatly concerned with the fast development of new photothermal nanomaterials. Herein we propose a photothermal immunoassay (PTIA) by taking mycotoxins (AFB1) as an example based on the PTEs of plasmonic Cu2–xSe nanocrystals (NCs). By loading plasmonic Cu2–xSe NCs into liposomes to form photothermal soft nanoballs (ptSNBs), on which aptamer of AFB1 previously assembled, a sandwich structure of AFB1 could be formed with the aptamer on ptSNBs and capture antibody. The heat released from the ptSNBs under NIR irradiation, owing to the plasmonic photothermal light-to-heat conversion through photon–electron–phonon coupling, makes the temperature of substrate solution increased, and the increased temperature has a linear relationship with the AFB1 content. Owing to the large amounts of plasmonic Cu2–xSe NCs in the ptSNBs, the PTEs get amplified, making AFB1 higher than 1 ng/mL detectable in food even if with a rough homemade immunothermometer. The proposal of PTIA opens a new field of immunoassay including developing photothermal nanostructures, new thermometers, PTIA theory, and so on.
研究领域:
纳米技术
·
分析化学
·
食品安全
·
生物物理学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）