Aquaculture Reports
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2020
·
IF 2.289
Liping Yang,Shaoyang Zhi,Junyi Hu,Wenlei Zhang,Yuru Zhang,Chaobin Qin,Guokun Yang,Xiao Yan,Guoxing Nie
Orexins are neuropeptides involved in energy homeostasis. In fish, the function of orexin-A in glucose metabolism is less reported. The present study aims to identify the potential role of orexin-A in the glucose absorption and metabolism in common carp. The full length of orexin cDNA was cloned using RT-PCR and RACE method. The prepro-orexin cDNA was 513 bp containing an open reading frame of 474 bp and encoded 157 amino acids which including 47 amino acids of orexin-A and 28 amino acids of orexin-B. The tissue distribution results showed that orexin was expressed in multiple tissues, especially with high-level expression in hypothalamus. The exogenous orexin-A protein was chemical synthesized. Intraperitoneal injection of orexin-A alleviated the increase of plasma glucose level at 1 h and 2 h after OGTT (Oral glucose tolerance test). Orexin-A also decreased glucose uptake activity and the expression of sglt1 and glut2 mRNA in intestine. However, orexin-A significantly increased the mRNA expression of glut2 in liver and glut4 in muscle. Moreover, the gluconeogenesis-related gene fbpase mRNA was significantly down-regulated, while glycolysis-related genes gk and hk were significantly up-regulated after treatment with orexin-A in vivo and vitro. These results implied that hyperglycemia could be alleviated in common carp after the injection of orexin-A and suggest a role of this peptide as a plasma glucose regulator by inhibition glucose absorption in intestine, promotion glucose transport to liver and muscle. Furthermore, hepatic gluconeogenesis was inhibited. Taken together, this study helped to understand the characterization of prepro-orexin and the function of orexin-A in fish glucose homeostasis.
研究领域:
神经科学
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分子生物学
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内分泌学
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水产养殖
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产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 4
·2129
·
IF 4.614
Xin Kang,Dongpeng Li,Xiaoxiang Zhao,Yanfeng Lv,Xi Chen,Xinshan Song,Xiangyu Liu,Chengrong Chen,Xin Cao
Phenanthrene (PHE) is a typical compound biomagnified in the food chain which endangers human health and generally accumulates from marine life. It has been listed as one of the 16 priority PAHs evaluated in toxicology. In order to evaluate the changes ofCYP1A GSTmRNA expression and EROD GST enzyme activity in carp exposed to lower than safe concentrations of PHE. Long-term exposure of carp to PHE at lower than safe concentrations for up to 25 days. The mRNA expression level and cytochrome P450 (CYP1A/EROD (7-Ethoxylesorufin O-deethylase)) and glutathione S-transferase (GST) activity were measured in carp liver and brain tissue. The results showed that PHE stress induced low-concentration induction and high-concentration inhibition ofCYP1Aexpression and EROD enzyme activity in the liver and brain of carp. In both two organs, GST enzyme activity was also induced. However, the expression ofGSTmRNA was first induced and then inhibited, after the 15th day. These results indicate that long-term exposure to PHE at lower than safe concentrations still poses a potential threat to carp’s oxidase system and gene expression.Keywords:Cyprinus carpio;phenanthrene ecotoxicology;cytochrome P4501A;7-ethoxylesorufin O-deethylase;glutathione S-transferase
研究领域:
环境毒理学
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分子生物学
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水生生态学
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产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2021
·Vol. 232
·期 9
·1-21
·
IF 2.52
Cano-Viveros Selene,Galar-Martínez Marcela,Gasca-Pérez Eloy,García-Medina Sandra,Ruiz-Lara Karina,Gómez-Oliván Leobardo Manuel,Islas-Flores Hariz
Aluminum, mercury, and iron have been found in high concentrations in various freshwater bodies around the world and have been shown to be very harmful contaminants to hydrobionts, causing metabolic dysfunction and damage at various levels. However, studies on mixtures of these pollutants are scarce, particularly in younger or developing organisms, which are more sensitive to damage. Therefore, the objective of this work was to evaluate the toxicity of Al, Fe, and Hg, in isolation and in mixture, on common carp embryos exposed to the maximum permissible limits described in the Mexican regulations for the protection of aquatic life, correlating the biomarkers of oxidative stress with the effects on embryonic development. For this purpose, the Cyprinus carpio embryos were exposed to iron (0.1 mg L −1 ), mercury (0.00001 mg L −1 ), aluminum (0.05 mg L −1 ), and their mixture, throughout their development and until hatching. The activity of the antioxidant enzymes (SOD, CAT, and GPx), the degree of lipoperoxidation, and the content of hydroperoxides and total proteins, as well as the morphological development of the embryos were evaluated at 12 h, 24 h, 48 h, 72 h, and 96 h of exposure. The results showed that the metals under study are toxic to C. carpio embryos and that their interaction modifies the toxic response. Thus, iron generates alterations in the activity of antioxidant enzymes and modification in embryonic development to a lesser extent than aluminum and the mixture of metals, while mercury exerts its toxicity on embryos by mechanisms other than oxidative stress, but the modification to the activity of antioxidant enzymes may contribute to the changes observed in embryonic development. The changes in the response of biomarkers of embryotoxicity and oxidative stress suggest that the combination of metals produces an antagonism-type interaction, so this study provides a precedent for future research to determine the type of interaction that a mixture of contaminants generates in developing aquatic organisms. Graphical abstract
研究领域:
环境毒理学
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水产生物学
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生态学
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DOI
高影响因子
Aquaculture Reports
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2023
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IF 3.7
X. Tian,Y.S. Shan,N.N. Peng,X. Ma,X. Shi,H. Li,X.J. Li,X.L. Meng
The evolution of teleost coloration is strongly linked with the fish-specific genome duplication (FSGD). Sepiapterin reductase (Spr) is commonly required in pteridine pigment synthesis and two paralogs are found in teleosts. However, the functions of spra and sprb in pigmentation have not been completely elucidated. Here we found that spra was intensively expressed in skin, fins and scales of koi carp, while sprb was ubiquitously expressed in all tissues . Then, the Spr enzyme activity inhibitor, sulfapyridine (SFD), was used to explore the phenotype and genes variation after spr inhibition. Following the administration of SFD, spra was significantly suppressed but sprb was up-regulated in skin. Additionally, the color phenotypes shifted from red to yellow due to a reduction in carotenoid concentration and the number of xanthophores/erythrophores in scales. Furthermore, the genes involved in carotenoid metabolism were influenced. However, pteridine metabolism-related genes rarely exhibited obvious changes. The results indicate that spra inhibition resulted in the dysregulation of carotenoid metabolism and pigment cells reduction, whereas sprb was likely to maintain the conserved functions in pteridine synthesis for metabolic homoeostasis. This study provided evidences for the different functions of spr paralogs in carotenoid and pteridine metabolism and for better understanding of the relationship between genome duplication and color pattern diversification.
研究领域:
生物学
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遗传学
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生物化学
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DOI
高影响因子
Fishes
·
2023
·Vol. 8
·期 12
·595
·
IF 2.3
Wenqian Wang,Shengyan Su,Ping Dong,Wenrong Feng,Jianlin Li,Chengfeng Zhang,Yongkai Tang
Photoperiod is one of the most important environmental cues for organisms, and it plays a crucial role in regulating feeding, behavior, growth, and metabolism. However, seasonal photoperiods are often overlooked in carp culture or experiments, with a poorly understood effect on lipid metabolism and oxidative stress in fish. To explore the effects of seasonal photoperiods, we exposed Huanghe carp (Cyprinus carpio haematopterus) to summer photoperiod (14 h light:10 h dark) and winter photoperiod (10 h light:14 h dark) daylight conditions in an eight-week experiment. Our results suggested that the winter photoperiod significantly increased the liver TG level as well as the transcript levels of genes related to lipid synthesis, indicating that the lipid metabolism in Huanghe carp liver was enhanced compared to summer photoperiod conditions, and that lipid deposition may be responsible for the increase in body weight level and hepatosomatic index. Additionally, MDA, GSH, GSH-PX, and T-AOC levels were significantly elevated in the liver of fish under the winter photoperiod, suggesting that Huanghe carp responded to winter photoperiod exposure-induced oxidative stress in the liver by enhancing the antioxidant response. Based on transcriptome analysis, the winter photoperiod activated hepatic autophagy response and the FOXO signaling pathway in Huanghe carp. Combined with the correlation analysis, the Huanghe carp maintains the physiological health of the liver by activating the FOXO signaling pathway-mediated cell cycle regulation and autophagy response in response to oxidative stress during winter photoperiod exposure. Our study provides the first evidence for the physiological regulation of the liver in Huanghe carp under seasonal photoperiod stimulation.
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DOI
高影响因子
ENVIRONMENT INTERNATIONAL
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2025
·
IF 9.7
Xiaojing Li,Huanqi Zhang,Peizhong Yu,Xiaozhe Chen,Yuhong Yang,Shiwen Xu
Chlorpyrifos (CPF), a typical organophosphorus pesticide, is frequently applied for pest control in households and agriculture worldwide. Liver, being the primary site for xenobiotic metabolism, is extremely vulnerable to drug threats. To elucidate the hepatotoxic mechanism of CPF and identify targets for its relief, the overlapping data between CPF-associated targets and liver injury-related genes were analyzed. It was found that CPF hepatotoxicity targets were mainly involved in oxidoreductase activity, fatty acid metabolism, glutathione metabolic process, response to biotic stimulus, ferroptosis, and mitochondrion disorders. TP53 molecular exhibited the strong binding stability with CPF, and TP53-mediated SLC7A11/GPX4 signaling suppression contributed to CPF-induced hepatotoxicity in Cyprinus carpio . CPF disrupted iron homeostasis by downregulating FTH, NCOA4, and FPN expressions, while simultaneously increasing ACSL4 expression to promote the accumulation of lipid peroxides MDA and LPO. Meanwhile, CPF reduced intracellular GPX4 levels, thereby diminishing lipid ROS scavenging capacity and triggering ferroptosis. Selenium (Se) could reverse CPF- and Erastin-induced ferroptosis and mitochondrial dysfunction. GSH depletion and GPX4 silencing reversed the alleviating effect of Se on CPF-induced lipid peroxidation, but they had little effect on iron contents. Meanwhile, Se addition also increased GSH contents and GPX4 protein expressions. The results revealed some targets of CPF-induced hepatotoxicity. These studies provided the theoretical basis for the future development of environmental monitoring and pollutant control strategies.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROTECTION
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2021
·
IF 2.077
Xiao Liu,Mingli Zhang,Xi Meng,Yanhong Bai,Xiuping Dong
ABSTRACT Plasma-activated water (PAW) is a new sanitizer that has received considerable attention for application in food industries. This research aimed to evaluate the effect of PAW on the inactivation of Shewanella putrefaciens and quality attributes of Yellow River carp ( Cyprinus carpio ) fillets. The carp fillet samples were immersed in sterile deionized water (SDW) or SDW activated by plasma discharge for 120 s (PAW120) for 1.5, 3.0, 4.5, or 6.0 min. After being treated by PAW120 for 6 min, the population of S. putrefaciens on carp fillets was significantly ( P < 0.05) decreased by 1.03 log CFU/g. Compared with SDW-treated samples, the L* value of PAW120–treated carp fillets was increased and the a* value was decreased after PAW120 treatment, whereas there was no significant ( P > 0.05) difference in the b* value. Compared with SDW, PAW120 caused no significant ( P > 0.05) changes in sensory properties and texture attributes of carp fillets including hardness, springiness, gumminess, and cohesiveness. However, 6-min PAW120 treatment caused a significant increase in the lipid oxidation level and a decrease in the pH value of the carp fillets. This work provides a basis for the potential application of PAW in the preservation of aquatic products.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2024
·
IF 4.2
Hongmin Lu,Yue Zhang,Xin Zhang,Ruoqi Wang,Tiantian Guo,Qi Wang,Hongjing Zhao,Mingwei Xing
Environmental pollution caused by arsenic or its compounds is called arsenic pollution. Arsenic pollution mainly comes from people mining and smelting arsenic compounds. In addition, arsenic compounds' widespread use and production of arsenic-containing pesticides, arsenic-rich water used to irrigate farms, or high arsenic levels in foods caused by coal burning are all sources of arsenic contamination. Arsenic contamination poses a significant threat to global public health. It is reported that exposure to arsenic can induce severe renal injury. However, the underlying mechanism needs to be clarified. In this study, the arsenic exposure model in vivo and in vitro was used to explore the mechanism of arsenic-induced renal injury, especially the role of ferroptosis and its regulatory mechanism, and then to evaluate its anti-pollution effect by supplementing zinc. The results showed that arsenic significantly induced ferroptosis, characterized by up-regulating the expression of YAP and TFR in kidney and CIK cells and then increasing the levels of Fe 2+ and ROS, lipid peroxidation, and iron metabolism. Microscopic observation revealed the shrinkage of mitochondria and the increase in membrane density. In addition, molecular docking and inhibitor experiments further confirmed that arsenic is involved in the process of ferroptosis by activating YAP and TFR. These results clarify the harmful effects of arsenic on carp kidneys and its mechanism and highlight the critical interactions between the YAP-TFR pathway, ROS, and ferroptosis. Importantly, this study found that zinc can reduce ferroptosis caused by the arsenic-activated YAP-TFR pathway by inhibiting YAP activation and lipid peroxidation.
研究领域:
环境科学
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毒理学
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水生生物学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·9209833
·
IF 3.9
Yuru Zhang,Haiying Fan,Yiman Zhang,Kedi Gao,Shibo Zhang,Xianglin Cao,Xinxin Xu,Ronghua Lu
Carbohydrates in aquaculture feeds can induce metabolic disturbance when exceeding fish utilization capacity, leading to lipid accumulation and insulin resistance. Bitter melon (BM; Momordica charantia ), rich in saponins, flavonoids, and polysaccharides, shows potential as a functional feed additive for glycemic control and lipid metabolism modulation. This investigation systematically assessed the effects of BM powder (BMP) supplementation (at 0.5%, 1%, and 1.5%) in high-starch (HG) diets for Cyprinus carpio . Compared to the HG group, BMP supplementation significantly reduced serum glucose (GLU) and triglycerides (TGs), while elevating total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C). The BMP group exhibited reduced malondialdehyde (MDA), enhanced antioxidation ability, and mitigated hepatopancreatic and intestinal histopathological damage from carbohydrate overload. Meanwhile, BMP restored muscle C20:3 n −6 and C22:6 n −3 (DHA) levels decreased by HG diets. Notably, 1.5% BMP decreased hepatopancreatic and muscular glycogen/lipid deposition, induced by a high-carbohydrate diet. Gene expression analysis revealed BMP upregulated glycolysis-related genes (glucokinase [ gk ], pyruvate kinase [ pk ], and pfk ) across tissues (hepatopancreas, muscle, intestine, and adipose tissue), while suppressing glycogen synthesis ( gys ) and gluconeogenesis ( g6pase ) genes. Besides, lipid biosynthesis genes were downregulated, corroborating reduced ectopic lipid storage. Taken together, these findings demonstrate that BMP supplementation significantly improves glycemic control, lipid metabolism, and antioxidant capacity in common carp. This suggests that BMP could serve as a natural, sustainable aquafeed additive to counter metabolic syndrome in intensively farmed fish.
研究领域:
水产动物营养
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鱼类代谢
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功能性饲料添加剂
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碳水化合物代谢
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脂质生理学
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抗氧化生物学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Zhiying Miao,Zhiruo Miao,Xiaohua Teng,Shiwen Xu
Lead (Pb) has been concerned as one of the most severe hazardous contaminants, because it can cause pyroptosis in multiple tissues of mammals and birds. Melatonin (Mel) has attracted much interest for its role in governing intestinal injury via microRNAs (miRNAs). To explore the effect of Mel on Pb exposure-induced intestinal epithelial cell pyroptosis in common carps by regulating miR-17-5p/TXNIP axis, the Pb exposure and Pb-Mel treated models were constructed in vivo . The results elucidated that the suppressed expression of miR-17-5p and intensified level of TXNIP were primarily detected in Pb-exposed gut tissues, and both abolished with Mel addition, along with downregulated Pb-mediated elevated expression of NLRP3, CASP1, IL1β and GSDMD. Additionally, the targeting relationship between miR-17-5p and TXNIP were demonstrated by dual-luciferase reporter assay, and on this basis, miR-17-5p NC, mimic and inhibitor cell models were established. Thereby, Thereby, the expression of TXNIP in the miR-17-5p mimic groups was significant lower in the Pb-exposure but still elevated than the Control group, and the expression of NLRP3 and NLRP3-dependent pyrotposis-related genes performed consistent alterations. Noticeably, the expression of TXNIP suppressed with Mel addition even in the miR-17-5p inhibitor cell model, resulting in the inactivation of NLRP3 inflammasome-dependent pyroptosis. Overall, we draw the conclusion as Mel attenuates Pb-induced intestinal epithelial cell pyroptosis via miR-17-5p/TXNIP axis. The present study provides a novel perspective for toxicological mechanism of Pb, and new insights for the detoxification mechanism of Mel.
研究领域:
毒理学
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分子生物学
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水生生物学
·
环境科学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
·
IF 4.7
Qian Wu,Wenrui Yang,Yanju Bi,Yujie Yao,Chengzhi Li,Xiaojing Li
Chlorpyrifos (CPF), a widely used organophosphate pesticide that has caused large-scale contamination globally, has become a major concern. Baicalein (BAI), as a flavonoid extract, shows anti-inflammatory as well as antioxidant activities . The kidneys of fish serve to excrete toxins and are major target organs for environmental contaminants. However, it is not obvious whether BAI can counteract the damage caused by CPF exposure to fish kidneys . Therefore, we conducted a 30-day simulation of CPF poisoning and/or BAI treatment by adding 23.2 μg/L CPF to water and/or 0.15 g/kg BAI to feed. In the transmission electron microscopy results, we observed obvious phenomenon of autophagy and apoptosis in the CPF group, and the TUNEL staining and immunofluorescence of LC3B and p62 double-staining results confirmed that CPF induced autophagy and apoptosis in the kidney of common carp . Furthermore, CPF induced the increase of ROS level and inhibition of PI3K and Nrf2 pathways, which in turn triggered oxidative stress , autophagy and apoptosis in carp kidney according to western blot , RT-qPCR and kit assays. However, addition of BAI significantly alleviated oxidative stress , autophagy and apoptosis due to binding to PI3K protein. Additionally, through phylogenetic tree and structural domain analyses, we also found that the binding sites of BAI and PI3K are conserved in a variety of representative species. These results suggest that BAI antagonizes CPF-caused renal impairments in carp involving the PI3K/AKT pathway and the Nrf2 pathway. Our findings provide new insights into the nephrotoxicity effects of CPF and the potential use of BAI as a detoxification agent for CPF intoxication.
研究领域:
环境毒理学
·
药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xin Du,Baohua Kong,Junjie He,Quanyu Zhang,Geer An,Tingting Zhang,Xiufang Xia
The inhibitory effect of water-tailored natural deep eutectic solvents (NADES) constructed from citric acid and trehalose with different amounts on the quality deterioration and oxidation of frozen-thawed (F-T) mirror carp ( Cyprinus carpio L. ) surimi was studied. NADES was obtained by citric acid to trehalose and the effect of moisture addition (v/v) on the structure, physicochemical, and anti-freezing capacity of NADES was assessed. NADES + 10 % H 2 O has relatively low viscosity (25 %) and strong freezing resistance. However, a 50 % H 2 O addition leads to the disappearance of the hydrogen bond. The addition of NADES effectively inhibits water loss, migration, and mechanical damage on F-T surimi. An inhibitory effect of 4 % (w/w) NADES on oxidation was verified by a decrease in carbonyl contents (17.4 %, 8.63 %) and TBARS (37.9 %, 15.2 %) of surimi compared with control ( P < 0.05) and sucrose + sorbitol after 5F-T cycles, suggesting the potential of NADES as a cryoprotectant for the food industry.
研究领域:
食品科学
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低温生物学
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水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）