ANALYST
·
2025
·
IF 3.3
Sihua Wu,Jinhui Zou,Baohua Zhang,Jiantian Lu,Guan Rong Lin,Yuwei Zhang,Li Niu
Nickel oxide (NiO) nanozyme, as an excellent oxidase mimic, have been widely used in fluorescence biological detection, water pollutant analysis, food safety and cell imaging. However, to fully achieve these applications, it is still a great challenge to regulate its crystalline micro/nanostructure and composites to achieve high enzyme activity and high specific surface area. Here, we applied a very simple thermal annealing treatment to restructure the calcined precursor of NiO. Importantly, it was found that oxygen vacancy (OV) concentration of the targeted NiO nanozyme is significantly increased while the annealing atmosphere is argon rather than air. Moreover, the as-prepared novel NiO sample (NiO-OV) nanosheets achieved ca. 2-fold enhancement in specific surface area. It is believed that the higher OV concentration and the larger specific surface area will increase the enzyme activity by accelerating electron transfer rate and increasing catalytic interfaces. The significant improvement of enzyme activity of NiO-OV was verified by the fluorescence "turn-on" experiment of Amplex red (AR). Finally, using NiO-OV/AR system, we constructed a highly sensitive enzyme sensor on L-Cys with a detection limit of 37.8 nM. It also displayed an excellent specificity for ten typical amino acid interferences.
研究领域:
生物医学工程
·
分析化学
·
材料科学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Zhuo Tan,Baoling Chu,Chenguang Wang,Jing Tian,Yi Nie,Hongshuai Gao
Efficient recovery and high-value utilization of keratin from waste human hair are of significant interest for sustainable materials development. However, the densely cross-linked disulfide bonds and extensive hydrogen-bond network of keratin make its mild and efficient extraction challenging. In this work, a redox-active deep eutectic solvent (DES) composed of L-cysteine (L-Cys) and monoethanolamine (MEA) was developed for keratin recovery from waste human hair. Under optimized conditions of 80 °C, 3.5 h, and 10 wt% solid loading, the keratin recovery yield reached 96.5%, and the solvent maintained stable performance over five recycling cycles. Experimental results, together with DFT and MD simulations, indicate that the dissolution process is driven by synergistic disulfide bond cleavage and disruption of the hydrogen-bond network, while the protein backbone remains largely intact. The recovered keratin was further phosphorylated and co-regenerated with cellulose to fabricate a phosphorylated keratin/cellulose composite membrane (P-KCM). The membrane exhibited efficient and selective Fe 3+ adsorption, with a maximum adsorption capacity of 297.8 mg·g −1 . FT-IR and XPS analyses reveal that phosphate groups serve as the primary coordination sites for Fe 3+ . In tests with real LiFePO₄ leachate, P-KCM removed 97.3% of Fe 3+ while leaving 80.1% of Li + in solution, and it retained 84.7% of its initial adsorption capacity after four adsorption-desorption cycles. This work provides a mild, efficient, and recyclable strategy for keratin valorization and offers a feasible approach for selective metal ion separation and pre-treatment in battery recycling.
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高影响因子
Nanoscale
·
2020
·Vol. 12
·期 24
·13010-13016
·
IF 6.895
Qiaoling Liu,Xiuyun Niu,Yan Zhang,Ying Zhao,Kaixin Xie,Boru Yang,Qing He,Shiyou Lv,Lin Li
The abnormal concentrations of both biothiols and pH in lysosomes are seriously related to many major diseases, such as Parkinson's and Alzheimer's diseases. Up to now, there are few reports that clearly illustrate the relationship between lysosomal pH and biothiols via fluorescence assay. Herein, novel carbon dots (Scy-CDs) are prepared with good water dispersibility and excellent photostability, and a large Stokes shift of 106 nm is exhibited under an excitation wavelength of 450 nm. The remarkable pH-dependent behavior of Scy-CDs is presented with the fluorescence quenching based on the donor-excited photoinduced electron transfer (d-PET) process. The pKa value is 5.30, which is in good agreement with the range of the normal and abnormal lysosomal pH. Upon the addition of cysteine (Cys) or homocysteine (Hcy), the d-PET process is effectively inhibited with fluorescence recovery totally. The significant co-localization of Scy-CDs with Lyso-Tracker Deep Red in HEp-2 cells and the Pearson correlation coefficient 0.88 strongly suggest that the Scy-CDs can target lysosomal pH and Cys/Hcy in living cells.
研究领域:
细胞生物学
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医学
·
分析化学
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DOI
PubMed
高影响因子
SENSORS
·
2023
·Vol. 23
·期 13
·5814
·
IF 3.9
Jing Liu,Xiaozong Dou,Hongyan Zhang
In this paper, a 2-mercaptobenzimidazole-copper nanoparticles (MBI-CuNPs) fluorescent probe with high performance based on 2-mercaptobenzimidazole functionalized copper nanoparticles was synthesized by a hydrothermal method and used for cysteine (Cys) detection in serum. The MBI-CuNPs probe exhibits strong fluorescence emission at 415 nm under the excitation at 200 nm, which is attributed to the metal-ligand charge transfer (MLCT) transition through the coordination of an MBI ligand and monovalent copper. Furthermore, the MBI-CuNPs probe has a high quenching fluorescence response to Cys, and shows a good linearity relationship with Cys in 0.05–65 µM, with a detection limit of 52 nM. Moreover, the MBI-CuNPs probe could eliminate the interference of biological mercaptan Hcy and GSH with a similar structure and reaction properties, due to the strong electron-donating ability of Cys, which can quench the fluorescence of the MBI-CuNPs probe. The MBI-CuNPs probe was applied to the analysis of Cys in real serum, and the absolute recovery rate was as high as 90.23–97.00%. Such a fluorescent probe with high sensitivity and selectivity has potential applications for the early prevention of various diseases caused by abnormal Cys levels.Keywords:fluorescent probe;monovalent copper;metal-ligand charge transfer transition;cysteine detection;serum
研究领域:
分析化学
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生物化学
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纳米技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-8
·
IF 5.479
Ma Xiaoming,Wang Zhen,He Shan,Zhao Jingyi,Lai Xiaoqi,Xu Jianguo
A fluorescent probe is described for detection of mercury(II) ion by using L-cysteine-modified gold nanoparticles (Cys-AuNP). These were fabricated by a tube-based redox reaction where Cys acts as both the reducing reagent and capping ligand. The Cys-AuNP display red fluorescence, with excitation/emission peaks at 373/625 nm. Owing to the high-affinity of the Hg(II)–Au(I) interaction and the Hg(II)/carboxy or amino group interaction, the presence of Hg(II) cause selective quenching the fluorescence, while other metal ions do not give such an effect. Based on these findings, a method was designed for the determination of Hg(II) that has attractive figures of merit. These include a low limit of detection (1.3 nM), a wide detection range (from 2 nM to 30µM), and excellent specificity. The method was applied to Hg(II) screening in (spiked) tap and river water, and it gave satisfactory results.
Schematic representation of the application of L-cysteine modified gold nanoparticles (Cys-AuNP) for qualitative and quantitative detection of mercury(II) ions. Based on the interaction between Cys-AuNP and mercury(II) ion to quench the red fluorescence of Cys-AuNP, the target mercury(II) can in turn be determined by a fluorometric method.
研究领域:
分析化学
·
环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Junchao Xu,Jian Pan,Yue Zhang,Jinbiao Liu,Lintao Zeng,Xuguang Liu
Aberrant levels of glutathione (GSH) and cysteine (Cys) are key risk indicators for some diseases, hence monitoring the levels of GSH and Cys is of great significance in clinical diagnosis. Herein, a water soluble near-infrared fluorescent probe ( CFL-NDB ) was readily prepared for fast, sensitive and discriminative detection of GSH and Cys. The probe was constructed with a near-infrared semi-heptamethine cyanine attached to 7-nitrobenzofurazan (NBD) via an ether bond. Upon substitution of NBD with GSH, this probe exhibits a significant fluorescence “turn-on” signal towards GSH at 702 nm with fast response (within 5 min) and low limit of detection (81 nM). In contrast, the probe not only displays the emission at 702 nm but also shows another emission band at 540 nm in the presence of cysteine (Cys). Therefore, this probe can be used to simultaneously detect Cys and GSH from different emission channels. Such capability of the probe has been demonstrated for quantitative determination of Cys and GSH in human plasmas. Furthermore, fluorescence imaging of HeLa cell indicated that the probe could be used for visualizing GSH and Cys in living cells.
研究领域:
生物化学
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医学诊断
·
荧光成像
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121881
·
IF 7.5
Qun Liu,Jiexiong Zhong,Huaiyu Wang,Shiqi Song,Xiaohui Xu,Wei Yang,Min Lu
The thorough removal of Cu 2+ from water poses a formidable challenge in environmental remediation. To overcome the performance limitations of traditional adsorbents that rely on physical pores or single functional groups. This study successfully constructs a hierarchical mesoporous composite, denoted as Cys-PDA@CoNi-LDH (where Cys, PDA, and LDH refer to L -cysteine, polydopamine, and layered double hydroxide, respectively). It is synthesized by grafting Cys onto a PDA framework via Michael addition, followed by in situ growth of CoNi-LDH nanosheets. The composite exhibits a high Cu 2+ adsorption capacity of 295.3 mg·g −1 and a removal efficiency of 92.2 % under optimal conditions (318 K, pH=6), with strong resistance to ionic interference. The synergistic mechanism involves specific -SH coordination, LDH ion exchange, surface complexation, and mesoporous confinement. Efficient desorption (86 % recovery) is achieved using 0.1 mol·L −1 HCl, and over 75 % of the initial capacity is retained after five cycles. Fixed-bed column experiments confirm its practical potential, with breakthrough curves well-fitted by Thomas and Yoon-Nelson models (adjusted R 2 = 0.986–0.999). Notably, it achieves a treatment capacity of 1.7 L·g −1 for low-concentration Cu 2+ (5 mg·L −1 ). This work provides a promising strategy for designing selective and renewable adsorbents for water purification.
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guo Wei,Chuanliang Wang,Rui Wang,Xuhui Geng,Jinhua Li,Lingxin Chen,Zhihua Song
In this study, a novel carbon dots-based system was developed for the sequential quantification of Au 3+ and L-cysteine (L-Cys). The system comprises N,F-doped carbon dots (N,F-CDs), a custom-designed miniaturized fluorimeter, and test strips. The N,F-CDs exhibit outstanding optical properties, including a large Stokes shift (127 nm) and high fluorescence intensity. The test strips offer low-cost, low-volume sample consumption, while the miniaturized fluorimeter provides high sensitivity in a compact format. The system demonstrated excellent analytical performance, including high sensitivity (limits of detection: 0.048–0.488 μmol/L for Au 3+ and 8.7–40.9 nmol/L for L-Cys), minimal matrix interference (within ±20 %), high accuracy (97.5 % to 104.5 % for Au 3+ , 97.6 % to 107.5 % for L-Cys), good precision (2.9 % to 4.5 % for Au 3+ , 1.2 % to 7.0 % for L-Cys), robust stability, and strong inter/intra-day reproducibility (relative standard deviations ≤2.1 %). Furthermore, it showed high selectivity for Au 3+ and L-Cys in complex matrices such as tap water, milk, and lemon-flavored beverages.
研究领域:
分析化学
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纳米技术
·
环境监测
·
生物分析
·
材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 14
·7955-7960
·
IF 3.049
Hui Zhang,Lizhen Xu,Wenxiu Li,Wenqiang Chen,Qi Xiao,Jun Huang,Chusheng Huang,Jiarong Sheng,Xiangzhi Song
A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.
研究领域:
分析化学
·
细胞生物学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jinqiu Li,Fanghao Wang,Hao Zhang,Duxia Cao,Ruifang Guan
The recognition of amino acid enantiomers is of great significance in drug research and disease diagnosis. However, the similar physical and chemical properties of enantiomers make chiral recognition challenging. Optical characterization is a promising approach to distinguish enantiomers. Carbon dots (CDs) have attracted widespread attention due to their simple synthesis, high economic benefits, and good biocompatibility. Chiral carbon dots, as fluorescent probes, have gradually received more attraction. Here, a fluorescence probe (CYS-CDs + Cu 2+ ) was constructed using D-Cys as chiral source to synthesize chiral carbon dots (CYS-CDs), and Cu 2+ as inducer to recognize the chirality of lysine enantiomers(L-/D-Lys). And then, based on the ability of Cu 2+ to quench the fluorescence of CYS-CDs and L-Lysine (L-Lys) could restore the fluorescence of the CYS-CDs + Cu 2+ probe, so achieve an “on–off-on” detection mode to detect L-Lys. On the contrary, D-Lys cannot restore the fluorescence of CYS-CDs + Cu 2+ . The probe can distinguish lysine enantiomers not only by fluorescence spectra but also by circular dichroism spectra. And, the probe can be used for the quantitative detection of L-Lys by fluorescence method, of which the detection range is 0–520 μM, and the limit of detection is only 13.70 μM. In addition, a paper-based fluorescent senor was constructed on this basis. This work may be of practical significance to expand the recognition method of amino acid enantiomers.
研究领域:
生物化学
·
分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2015
·Vol. 62
·期 8
·739-746
·
IF 0.648
Feng Li,Yaqin Chai,Ruo Yuan,Xin Li,Yuqiao Yang
In this study, a nanocomposite of 3, 4, 9, 10-perylenetetracarboxylic acid and L-cysteine (PTCA-Cys) with satisfactory water-solubility and film-forming ability was prepared and worked as substrate for modifying the glassy carbon electrode. Then, gold nanoparticles (AuNPs) were immobilized to achieve a PTCA-Cys-AuNPs modified electrode which provided more reaction positions on the sensor. Scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and different pulse voltammetry were employed to characterize the assembly process of the sensor. The constructed sensor displayed desirable sensitivity, selectivity and stability towards the simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Under the optimal experimental conditions, the oxidation peaks of AA, DA and UA appeared at 64, 240 and 376 mV, respectively. The corresponding linear response ranges were 3.2–435, 0.04–100 and 0.80–297 μM, and the detection limits were 1.1, 0.010 and 0.27 μM (S/N=3), respectively.
研究领域:
电分析化学
·
纳米材料
·
生物传感器
·
环境与生物医学分析
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DOI
高影响因子
LANGMUIR
·
2017
·
IF 3.833
Tingting Zhou,Jiagen Li,Xu Jia,Xubo Zhao,Peng Liu
Novel biocompatible and biodegradable pH/reduction dual-responsive oxidized alginate-doxorubicin (mPEG-OAL-DOX/Cys) prodrug nanohydrogels were designed for tumor-specific intracellular triggered release of anticancer drug DOX by conjugating DOX via acid-labile Schiff base linkage into the PEGylated oxidized alginate (mPEG-OAL) cross-linked with bioreducible disulfide bond. The effect of the complexation with cyclodextrins (α-CD and β-CD) before or after the cross-linking of the mPEG-OAL on the DOX content and controlled release performance was investigated. It was found that the cyclodextrin inclusion complex prodrug nanohydrogels mPEG(CD)-OAL-DOX/Cys, prepared by cross-linking of the mPEG-OAL after complexation with cyclodextrins, exhibited better pH/reduction dual-responsive controlled release performance than the mPEG-OAL-DOX/Cys(CD) ones prepared by cross-linking of the mPEG-OAL before complexation with cyclodextrins, owing to the supramolecular cross-linking of the adjacent pseudopolyrotaxanes. Especially for the cyclodextrin inclusion complex prodrug nanohydrogels mPEG(α-CD)-OAL-DOX/Cys, DOX was released rapidly under lower pH media mimicking the tumor microenvironment and completely released within 48 h, while the premature leakage under the simulated physiological condition was ∼40%, without burst release in both cases. The cellular toxicity and uptake results demonstrated that the mPEG(α-CD)-OAL-DOX/Cys prodrug nanohydrogels possessed similar inhibition against cancer cell growth in comparison with the free DOX and enhanced drug intracellular accumulation.
研究领域:
药剂学
·
生物材料
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）