INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Huayang Tang,Yian Chen,Dexun Fan,Fengguang Zhao,Shuangyan Han
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing customized strategies for MOF immobilization of enzymes remains challenging. In this study, we introduce a designable strategy involving the construction of bimetal-organic frameworks (ZnCo-MOF) based on metal ions compatibility. The DAE@MOFs materials were prepared and characterized, and the immobilization of DAE and the enzymatic characteristics of the MOF-immobilized DAE were subsequently evaluated. Remarkably, DAE@ZnCo-MOF exhibited superior recyclability which could maintain 95 % relative activity after 8 consecutive cycles. The storage stability is significantly improved compared to the free form, with a relative activity of 116 % remaining after 30 days. Molecular docking was also employed to investigate the interaction between DAE and the components of MOFs synthesis. The results demonstrate that the DAE@ZnCo-MOF exhibited enhanced catalytic efficiency and increased stability. This study introduces a viable and adaptable MOF-based immobilization strategy for enzymes, which holds the potential to expand the implementation of enzyme biocatalysts in a multitude of disciplines.
研究领域:
生物化学
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食品科学
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材料科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Jinqiu Li,Fanghao Wang,Hao Zhang,Duxia Cao,Ruifang Guan
The recognition of amino acid enantiomers is of great significance in drug research and disease diagnosis. However, the similar physical and chemical properties of enantiomers make chiral recognition challenging. Optical characterization is a promising approach to distinguish enantiomers. Carbon dots (CDs) have attracted widespread attention due to their simple synthesis, high economic benefits, and good biocompatibility. Chiral carbon dots, as fluorescent probes, have gradually received more attraction. Here, a fluorescence probe (CYS-CDs + Cu 2+ ) was constructed using D-Cys as chiral source to synthesize chiral carbon dots (CYS-CDs), and Cu 2+ as inducer to recognize the chirality of lysine enantiomers(L-/D-Lys). And then, based on the ability of Cu 2+ to quench the fluorescence of CYS-CDs and L-Lysine (L-Lys) could restore the fluorescence of the CYS-CDs + Cu 2+ probe, so achieve an “on–off-on” detection mode to detect L-Lys. On the contrary, D-Lys cannot restore the fluorescence of CYS-CDs + Cu 2+ . The probe can distinguish lysine enantiomers not only by fluorescence spectra but also by circular dichroism spectra. And, the probe can be used for the quantitative detection of L-Lys by fluorescence method, of which the detection range is 0–520 μM, and the limit of detection is only 13.70 μM. In addition, a paper-based fluorescent senor was constructed on this basis. This work may be of practical significance to expand the recognition method of amino acid enantiomers.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
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2025
·
IF 9.8
Xuhui Li,Menghan Zhang,Mengyang Yin,Xingming Ning,Zhongwei An,Xinbing Chen,Pei Chen
Advancing flexible zinc-air batteries (ZABs) hinges on efficient bifunctional oxygen electrocatalysts, yet resolving the intrinsic activity trade-off between oxygen reduction (ORR) and evolution reactions (OER) remains a long-standing scientific challenge. Herein, we propose a molecular engineering strategy to optimize the electronic configuration of Fe centers in an iron‑cobalt tetranitro-phthalocyanine complex (FeCoPc-NO 2 ), by Co atoms and electron-withdrawing nitro groups. Density functional theory and electrochemical characterizations unveil that a weak d-d coupling between adjacent Fe/Co atoms tailors the Fe 3d-orbital electron density and the adsorption energies of oxygen-containing intermediates, boosting OER activity (η 10 = 320 mV) and concurrently retaining high ORR performance (E 1/2 = 0.81 V vs. RHE). Moreover, the assembled flexible ZABs achieve a peak power density of 74.9 mW·cm −2 , high round-trip efficiency (65.8 %), prolonged cycling stability (48 h), and mechanical robustness (>90° bending). This study validates the feasibility of atomically precise molecular design in creating bifunctional electrocatalysts with exceptional performance and cost efficiency.
研究领域:
材料科学
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电化学
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纳米催化
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能源存储与转换
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分子工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Dan Zhao,Jingjing Huang,Juan Li,Xiao Ma,Fang Wang,Honglei Zhang,Jiaze Xie,Jian Sun,Chuanxia Chen
Dual-readout protocols with two signal transduction channels can significantly improve the detection precision and diversity. In contrast to the previous tedious and time-consuming preparation of two response materials, a convenient and commercially available small molecule o -Phenylenediamine (OPD)-based dual-mode system was developed for the ultrasensitive detection of D-penicillamine (D-PA). Fortunately and interestingly, it is demonstrated that chloride ions (Cl − ) could largely enhance the oxidative ability of copper ions (Cu 2+ ) and improve the reaction rate and efficiency of the Cu 2+ -OPD system, leading to the boosted colorimetric and fluorescent signals and shorter reaction time. Meanwhile, D-PA could strongly bind with Cu 2+ to form a stable Cu 2+ -D-PA complex, thereby inhibiting the aforementioned oxidation process and exhibiting decrease of absorbance values and fluorescence intensity. Inspired by the excellent analytical performance, an “INH” molecular logic gate was devised by employing Cl − and D-PA as the inputs, and the dual-mode strategy has been successfully employed to probe D-PA in tablets and human serum samples. Such cost-effective yet efficacious analytical tool not only improve the sensing performance of the Cu 2+ -OPD reaction system, but also enhance the detection sensitivity for D-PA and promote the application and development of OPD-based reactions.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2025
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IF 3.6
Jin Li-Qun,Liu Meng-Dan,Guan Zi-Yu,Li Yi-Xin,Xue Ya-Ping,Liu Zhi-Qiang,Zheng Yu-Guo
DAAO is applied as a potential catalyst in the biosynthesis of L-PPT. However, its low solubility expression constrains its broader industrial application. Herein, a novel DAAO derived from Cladophialophora carrionii ( Cc DAAO) was identified, which demonstrated superior catalytic performance toward D-Ala (specific activity: 106.38 ± 1.21 U/mg, K m : 1.56 ± 0.06 mM), along with remarkable thermostability and broad substrate spectrum. Under optimal culture conditions, the soluble expression level of Cc DAAO was enhanced through a co-expression strategy with molecular chaperones, and the enzyme activity increased by 36.3% compared with the initial level. Subsequently, Cc DAAO was constructed as a fusion protein (CGD) with catalase from Geobacillus sp. CHB1 ( Gb CAT) and applied in a D-amino acid aminotransferase (DAAT)-mediated cascade system. In a 2 L reaction system, this cascade system achieved complete conversion (> 99%) of 1 M D,L-PPT within 8 h, exhibiting a yield of 11.26 g/L/h for PPO, which represents a significant improvement over existing reports. This study presents a promising practical approach for the industrial production of optically pure L-PPT.
研究领域:
生物催化
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酶工程
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药物合成
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工业微生物
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
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2024
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IF 3.9
Wenhao Xia,Shimeng Liu,Lihui Huang,Hao Wu,Tao Bai,Shanshan Wang,Fei Hao,Xiao Wang,Wen Wang
d -tagatose, a rare sugar with broad applications in the food and pharmaceutical industries, can be synthesized from d -fructose by epimerases, an approach with significant biosynthesis potential. However, so far there has been only one attempt to engineer tagatose 4‑epimerase through random mutagenesis. The lack of exploration has severely restricted the application of epimerases in the industrial production of d -tagatose. The previous research primarily focused on enhancing the catalytic activity of epimerases through random mutagenesis, with limited understanding of how substrate-adjacent residues affect enzyme efficiency, which was hence the subject of this study. First, using a semi-rational design approach, after three rounds of mutagenesis and screening, the mutant (S125D/S268A/Q124E) of D-tagaturonate epimerase (UxaE) from Thermotoga neapolitana was obtained, which increased d -tagatose production efficiency by 10.8-fold compared to the wild type, with a specific activity of 1505.6 U/g, representing the highest specific activity reported for a UxaE mutant to date. Molecular dynamics simulations showed that the substitution of Ser to Asp-125 enhanced substrate-enzyme interactions, while the substitution of Glu to Gln-124 reinforced the hydrogen bond network around the catalytic residue Glu-128, further reshaping the substrate-binding pocket and enhancing catalytic activity. This study for the first time explored the enhancement of the C4 epimerization function of epimerases by reshaping their substrate-binding pockets, thereby providing a feasible route for the industrial biosynthesis of d -tagatose.
研究领域:
生物化学
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生物技术
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酶工程
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分子生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2016
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IF 4.052
Baogang Xie,Yalan Liu,Huiqin Zou,Yong Son,Huiyun Wang,Haipeng Wang,Jianghua Shao
d -Glucaric acid (GA) derivatives exhibit anti-cancerogenic properties in vivo in apples, but quantitative information about these derivatives is limited. Hydrophilic interaction-based HPLC with ultraviolet detection or mass spectrometry was developed to quantify GA and/or d -glucaro-1,4-lacton (1,4-GL) in apples. Although the formation of 1,4-GL from GA could be the prerequisite to exert biological effects in vivo , only a small portion of GA (<5%) was identified and converted to 1,4-GL in the rat stomach. The 1,4-GL content in apples ranged from 0.3 mg/g to 0.9 mg/g, and this amount can substantiate health claims associated with apples. The amount of 1,4-GL was 1.5 times higher in Gala and the ratio of 1,4-GL to GA was lower in Green Delicious apples than those in the other varieties. Our findings suggested that the variety and maturity of apples at harvest are factors that determine 1,4-GL content.
研究领域:
食品科学
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植物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
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IF 3.858
Jianjiong Li,Shanshan Yu,Yingang Wang,Peiyuan Yao,Qiaqing Wu,Dunming Zhu
(S)-2-Aminobutane, d-alanine, and d-homoalanine are important intermediates for the production of various active pharmaceutical ingredients and food additives. The preparation of these small chiral amine or amino acids with high water solubility still demands searching for efficient methods. In this work, we identified an ω-transaminase (ω-TA) from Sinirhodobacter hungdaonensis (ShdTA) that catalyzed the kinetic resolution of racemic 2-aminobutane at a concentration of 800 mM using pyruvate as the amino acceptor, leading to the simultaneous isolation of enantiopure (S)-2-aminobutane and d-alanine in 46% and 90% yield, respectively. In addition, (S)-2-aminobutane (98% ee) and d-homoalanine (99% ee) were isolated in 45% and 93% yield, respectively, in the kinetic resolution of racemic 2-aminobutane at a concentration of 400 mM coupled with deamination of l-threonine by threonine deaminase. We thus developed a biocatalytic process for the practical synthesis of these valuable small chiral amine and d-amino acids.
研究领域:
生物催化
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有机化学
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药物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Xinrui Tang,Abdullah Arsalan,Guoyan Zhang,Junhua Yun,Cunsheng Zhang,Xianghui Qi
D-Allose, a rare sugar, has gained significant attention not only as a low-calorie sweetener but also for its anticancer, antitumor, anti-inflammatory, antioxidant, and other pharmaceutical properties. Despite its potential, achieving high-level biosynthesis of D-allose remains challenging due to inefficient biocatalysts, low conversion rates, and the high cost of substrates. Here, we explored the food-grade coexpression of Blautia produca D-allulose 3-epimerase (Bp-DAE) and Bacillus subtilis L-rhamnose isomerase (BsL-RI) within a single cell using B. subtilis WB800N as the host. Using this system, D-allose was synthesized via a simple, cost-effective, one-pot enzymatic process, employing whole cells as catalysts and D-fructose as the substrate. The system exhibited optimal activity at 65 °C, pH 8.5, with 1 mM Mn2+ and 20 g/L of whole-cell dry weight. Initial production reached 12.5 g/L D-allose with a 12.5% yield from 100 g/L D-fructose. Optimization of dual promoter combinations enhanced production, achieving 15.0, 29.1, and 43.2 g/L D-allose from 100, 200, and 300 g/L D-fructose, with yields of 15.00, 14.55, and 14.40%, respectively. This D-allose production biocatalyst offers a scalable and economically viable platform for the industrial production of rare sugar.
研究领域:
生物化学
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食品科学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 11
·1727
·
IF 4.7
Chengyu Lu,Ziwei Chen,Yuvaraj Ravikumar,Guoyan Zhang,Xinrui Tang,Yufei Zhang,Mei Zhao,Wenjing Sun,Xianghui Qi
L-Arabinose isomerase (L-AI) has been commonly used as an efficient biocatalyst to produce D-tagatose via the isomerization of D-galactose. However, it remains a significant challenge to efficiently synthesize D-tagatose using the native (wild type) L-AI at an industrial scale. Hence, it is extremely urgent to redesign L-AI to improve its catalytic efficiency towards D-galactose, and herein a structure-based molecular modification ofLactobacillus plantarumCY6 L-AI (LpAI) was performed. Among the engineered LpAI, both F118M and F279I mutants showed an increased D-galactose isomerization activity. Particularly, the specific activity of double mutant F118M/F279I towards D-galactose was increased by 210.1% compared to that of the wild type LpAI (WT). Besides the catalytic activity, the substrate preference of F118M/F279I was also largely changed from L-arabinose to D-galactose. In the enzymatic production of D-tagatose, the yield and conversion ratio of F118M/F279I were increased by 81.2% and 79.6%, respectively, compared to that of WT. Furthermore, the D-tagatose production of whole cells expressing F118M/F279I displayed about 2-fold higher than that of WT cell. These results revealed that the designed site-directed mutagenesis is useful for improving the catalytic efficiency of LpAI towards D-galactose.
研究领域:
生物化学
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食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Tingting Tian,Mingxia Liu,Lixia Chen,Fengjiao Zhang,Xin Yao,Hong Zhao,Xiangjun Li
For D-amino acid (DAA) electrochemical biosensors , it is necessary to achieve chiral recognition in racemic solutions or mixtures. However, common chiral recognition is only performed in a single isomer solution. Here, D-amino acid oxidase (DAAO) was used as a chiral selector, and carbon nanotubes (CNTs) as a signal amplifier to construct a non-mediator-style DAA biosensor. The biosensor showed high performance against enantiomer interference: in alanine (Ala) enantiomer mixtures, accurate quantification of D-Ala was achieved when the concentration ratio of L-Ala to D-Ala was 100. In Ala racemic solutions, the linear equation slope was almost consistent with that of standard D-Ala. This high performance was due to the combination of stereoselectivity (enzyme protein) and a catalytic reaction (redox center). The mechanism for the electrical signal change of the biosensor was explored and verified by cyclic voltammetry (CV). The results showed that (i) flavin adenine dinucleotide (FAD, redox center of DAAO) was a direct electroactive substance that produced a reduction peak current; in the presence of O 2 , the amount of FAD increased leading to an increase of the reduction peak current. (ii) In the presence of DAA, the chemical reaction FAD + DAA → imino acids + FADH 2 occurred and consumed FAD, which resulted in its decrease; thus, the reduction peak current also decreased. Under the same oxygen concentration, the linear decrease of the reduction peak current in the presence of DAA was due to FAD consumption. The biosensor was used for practical analyses in milk and urine samples with satisfactory results.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Deng Jun,Wu Sai,Yao Mengyun,Gao Changyou
Chirality is one of the ubiquitous phenomena in biological systems. The left handed (L-) amino acids and right handed (D-) sugars are normally found in proteins and in RNAs and DNAs, respectively. The effect of chiral surfaces at the nanoscale on cellular uptake has, however, not been explored. This study reveals for the first time the molecular chirality on gold nanoparticles (AuNPs) functions as a direct regulator for cellular uptake. Monolayers of 2-mercaptoacetyl-L(D)-valine (L(D)-MAV) and poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were formed on AuNPs surface, respectively. The internalized amount of PAV-AuNPs was several times larger than that of MAV-AuNPs by A549 and HepG2 cells, regardless of the chirality difference. However, the D-PAV-AuNPs were internalized with significantly larger amount than the L-PAV-AuNPs. This chirality-dependent uptake effect is likely attributed to the preferable interaction between the L-phospholipid-based cell membrane and the D-enantiomers.
研究领域:
纳米技术
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细胞生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）