JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Binbin Zhang,Jiao Ma,Kaide Ou,Xin Cong,Hao Zou,Yonggui Liao,Yajiang Yang,Hong Wang
By loading 3-nitrophenylboronic acid (3-NPBA) which can react with saccharide, the fluorescence lanthanide metallogel can emit fluorescence signals at 398, 490 and 546 nm. Thus, a three-dimensional responsive fluorescence sensor array (H 6 L/Tb 3+ /3-NPBA) was prepared to recognize saccharides. Due to the typical pH responsiveness of the fluorescence behavior of the sensor array, it is suitable for H + generation systems, such as the reaction of 3-NPBA with cis -diol of saccharides. The response of fluorescence signal intensity to 15 different saccharides was measured, and the qualitative discrimination of those saccharides was investigated by principal component analysis (PCA). The results showed that PCA elliptic clusters did not overlap, even though the concentration of the saccharides was as low as 3 mmol/L, and the discrimination rate of each saccharide reached 100 %. The “double blind” experiment indicated that the sensor system has good ability to discriminate unknown component samples. Furthermore, the discrimination rate of fructose/glucose mixtures with varied mole ratios also reached 100 %. The method of Euclidean distance confirmed that there was a good linear correlation between PCA elliptic clusters of fructose and its concentration, which could realize the quantitative analysis of fructose. The results of PCA indicated that the elliptic clusters of tea drinks with various sugars and commercially available sugar-containing drinks were loosely distributed, which further confirmed that the sensor system can effectively discriminate the saccharides under the interference of complex components.
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CATALYSIS LETTERS
·
2023
·
IF 2.8
Hao Runming,Du An,Zhu Qingling,Wu Xue,Liu Sijia,Wang Ke,Wang Yang
5-Hydroxymethylfurfural (HMF) is a high value-added platform compound, converted into bio-based polyesters and biofuels etc. In this paper, we used two commercially available hexamethylenimine and cetyltrimethylammonium bromide (CTAB) as the structure-directing agents to synthesize HMWW zeolite nanosheets via one step. A series of HMWW zeolite nanosheets with different disorder index ( DI ) were controllably prepared by adjusting the amount of CTAB, operating also as an in situ exfoliating agent, converting fructose into HMF in the tetrahydrofuran/NaCl-H 2 O biphasic solvent with low boiling point. Successful construction of disordered HMWW zeolite nanosheets was verified by PXRD. Further characterizations by BET, SEM and TEM etc. show that HMWW 4.0 with the optimum DI of 0.67 has a disordered network-like arrangement of layers with the external surface area of 224 m 2 /g and the mesoporous size of 3.5 nm. Catalytic evaluations showed that HMF selectivity was closely related to DI of the catalysts. Under the optimized DI , fructose molecules are readily accessible to acidic sites on the external surface while larger external surface area is exposed. The HMWW 4.0 zeolite catalyzed fructose dehydration in the biphasic solvent, producing 90% HMF selectivity with 93% fructose conversion at 140 °C for 6 h. In the reuses of the HMWW 4.0 catalyst, HMF selectivity was around 90% with fructose conversion decreasing by ca. 10% after six runs, indicating that it possesses the promising stability. Graphical Abstract
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Antioxidants
·
2022
·Vol. 12
·期 1
·104
·
IF 7.675
Xianchao Shang,Manman Zhang,Jing Hu,Yuqin Zhang,Long Yang,Xin Hou
Natural deep eutectic solvents (NADESs) have been gradually applied to green extraction of active ingredients. In this study, microwave-assisted NADESs were applied to the extraction of flavonoid compounds from perilla leaves. Through comparative experiments, NADES-3 (choline chloride and malic acid at a molar ratio of 1:1) was found to have the highest extraction efficiency of total flavonoids, including apigenin 7-O-caffeoylglucoside, scutellarein 7-O-diglucuronide, luteolin 7-O-diglucuronide, and scutellarein 7-O-glucuronide by HPLC-MS. The following optimal extraction parameters were obtained based on response surface design: water content in NADES of 23%, extraction power of 410 W, extraction time of 31 min, and solid–liquid ratio of 75 mg/mL, leading to the extraction yield of total flavonoids of 72.54 mg/g. Additionally, the strong antimicrobial and antiallergic activity, inhibition of nitrosation, and antioxidant activity of total flavonoids by using NADESs were confirmed. This new extraction method provides a reference for the further exploration of NADES systems and may be widely used for the green extraction of natural active ingredients.Keywords:Perillae folium;natural deep eutectic solvents;microwave-assisted extraction;chemical compositions;antimicrobial activity;response surface methodology
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Maofu Geng,Yuhong Tao,Zhenyu Zhang,Yuewen Dai,Juncheng Huang,Fei Liu,Haining Na,Jin Zhu
Through analysis of the transformation of carbon sources including glucose, fructose, and 5-hydroxymethylfurfural in hydrothermal carbonization (HTC) and the adsorption of carbon tubes (CNTs) with them, the formation mechanism of a hydroxyl-rich carbon layer on the CNT surface is clearly revealed. During HTC, the adsorption of furan and aromatic clusters and even primary particles by CNTs by π–π interaction is noticed as the key condition to improve the utilization rate of carbon sources and the quality of the carbon layer. The coated CNTs own a hydroxyl-rich carbon layer with a thickness of several nanometers on its surface, which provides the ability for functional applications. With the use of the hydroxyl-rich carbon layer-coated CNTs, a low-acid aqueous system is established for the hydrolysis of cellulose to sugar. Accordingly, the cellulose conversion and glucose yield are obviously improved. The route of HTC for hydroxylation of CNTs not only fundamentally avoids the use of strong acid oxidation in traditional method but also provides the useful guidance to prepare functional carbon materials with application prospects.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2023
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IF 3.4
HuaYang Si,Yimeng Chen,Jie Yang,Xiaodong Wen
Although the roots of Achyranthes bidentata (AB), Cyathula officinalis (CO) and Achyranthes aspera (AA) are different drugs, they are always confused in clinical practice due to their similar chemical components and functions. As polysaccharides are abundant in these drugs, a systematic comparison of polysaccharides from AB, CO and AA is not only necessary to understand their similar but not identical functions, but also helpful for the quality control of them. In this study, polysaccharides from 22 batches of AB, CO and AA were compared with monosaccharide composition , molecular weight distribution and saccharide mapping. Polysaccharides of AB, CO and AA had similar monosaccharide compositions but their relative contents of fructose, glucuronic acid , galacturonic acid and glucose were significant different, and could be used as key markers to distinguish them. Results from molecular weight distribution and saccharide mapping showed polysaccharides from AB, CO and AA were mainly composed of fructans with β-2,1 and β-2, 6- D -fructosidic linkages, but their degree of polymerization were different. Meanwhile, pectins were also contained in these three drugs. AB is partial to immunomodulation while CO is partial to removing blood stasis . Fructans and pectins are the similar bioactive substance basis of AB, CO and AA whereas their structural difference might be contributed to the efficacy differentia of these three drugs. This study provides a better understanding on the profiles of polysaccharides from AB, CO and AA, further guiding their clinical usage and facilitating their quality control.
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CARBOHYDRATE RESEARCH
·
2023
·
IF 3.1
Kuan Li,Jinling Wang,Yadong Zhuang,Guangxiang Yuan,Yuxiu Li,Xiaolan Zhu
N-(2-Deoxy- D -glucos-2-yl)- L -histidine (Glu-His), one of Heyns rearrangement products (HRPs), was prepared by condensation , dehydration and rearrangement using l -Histidine and d -Fructose as raw materials with methanol as solvent. The response surface method (RSM) was used to improve yield of product and the optimal reaction condition was as following: the original ratio of Fru:His was 1.2:1 and the temperature and time of reaction was 73.2 °C and 4.7 h, and the yield of final product was 74.10% with the purity of 99.7%. The structure of product was identified by IR, NMR and conformed as C 12 H 19 N 3 O 7 (317.1 Da) by high-resolution mass spectrometry (HRMS) and UPLC-MS/MS. The pyrolysis behavior of Glu-His showed that its initial pyrolysis temperature was 145.2 °C and the total weight loss reached 70.61% at 800 °C. The number of pyrolysis products increased with the increase of temperature, and the main pyrolysis products were pyrans , furans, pyrazines , pyrroles , pyridines, indoles and etc. with burnt-sweet, baking, nutty, sweet and floral aroma features. At last, the fragrance enhancement effect of Glu-His in the preparation of reconstructed tobacco stem (RTS) was investigated and the result of sensory evaluation showed that the smoke of RTS cigarettes brought about more sweet and moist, less irritation, better flavor and comfort with the addition of Glu-His (0.25%, w/w).
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TALANTA
·
2023
·
IF 6.1
Zhi-Yong Guo,Chen Zhang,Lin-min Chen,Mei-huang Zeng,Qiu-hong Yao,Ting-Xiu Ye,He-zhou Luo,Xiao-mei Chen,Xi Chen
Shell-isolated colloid plasmonic nanomaterials-based nanoreactor is a well-established platform widely applied in catalyst or Surface Enhanced Raman Scattering (SERS) sensors. The potentials versatility of nanoreactor platform is mainly implemented by the well-defined and tailorable structure of colloid plasmonic nanomaterials . Currently, a competitive conjugative-mediated nanoreactor is introduced to determine glucose with SERS. Glucose-conjugating nanoreactor, as convertors of the sensors, are constructed by coordinated deposition colloidal gold nanoparticles with sodium nitroprusside framework (Au@SNF) and covalently bonded 4-mercaptopyridine (4-Mpy) with self-assembly strategy. The nanoreactor contained the signal-amplifier Au@SNF NPs, conjugative-mediated signal receiver 4-Mpy, and signal internal standard molecular CN − . In addition to well-defined morphology and functionality, conjugative-mediated and internal standards method are also employed to benefit the nanoreactor. The two-parameter strategy significantly improves the signal indication and correction. Using this proposed platform, the competitive-mediated nanoreactor provides a quantitative SERS detection of glucose, and extends the applicability of SERS in more complicated and reproducibility analysis. Meanwhile, the nanoreactor based sensors also exhibited better properties to detect glucose in various food samples and bio-samples which provided strongly appliance for glucose sensors.
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VETERINARY MICROBIOLOGY
·
2023
·
IF 3.3
Lujie Zhang,Xing Liu,Jian Mao,Yangyang Sun,Yanni Gao,Juan Bai,Ping Jiang
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important causative agents in the pig industry worldwide, causing reproductive failure in sows and respiratory problems in growing pigs. Glucose metabolism is a major pathway for energy production and interacts with many cellular processes, such as innate immunity response. It is unclear whether PRRSV infection can use the glucose metabolic pathway to generate immune escape in favor of viral replication. Here, we found that high glucose promotes PRRSV replication and glycolysis, and inhibits poly(I:C)-induced RLR signaling. Conversely, inhibition of the glycolysis pathway significantly promoted poly(I:C)-induced RLR signaling and inhibited PRRSV replication, suggesting that glycolysis promotes PRRSV replication by inhibiting interferon signaling. Furthermore, PRRSV promotes glycolysis to produce lactate, which acts as a key metabolite to promote viral replication by inhibiting RLR signaling by targeting MAVS. And the glycolytic inhibitors targeting HK2 and LDHA in glycolysis could inhibit PRRSV replication. Taken together, these findings suggested that PRRSV infection promotes glycolysis to produce lactate, which targets MAVS to inhibit RLR signaling and thus promote viral replication. Our findings provide an insight into the pathogenesis of PRRSV and offer a theoretical basis for further development of antiviral therapeutic targets.
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CELL RESEARCH
·
2023
·
IF 44.1
Ai Yuan-li,Wang Wei-jia,Liu Fan-jian,Fang Wei,Chen Hang-zi,Wu Liu-zheng,Hong Xuehui,Zhu Yuekun,Zhang Ci-xiong,Liu Long-yu,Hong Wen-bin,Zhou Bo,Chen Qi-tao,Wu Qiao
Pyroptosis is a type of regulated cell death executed by gasdermin family members. However, how gasdermin-mediated pyroptosis is negatively regulated remains unclear. Here, we demonstrate that mannose, a hexose, inhibits GSDME-mediated pyroptosis by activating AMP-activated protein kinase (AMPK). Mechanistically, mannose metabolism in the hexosamine biosynthetic pathway increases levels of the metabolite N -acetylglucosamine-6-phosphate (GlcNAc-6P), which binds AMPK to facilitate AMPK phosphorylation by LKB1. Activated AMPK then phosphorylates GSDME at Thr6, which leads to blockade of caspase-3-induced GSDME cleavage, thereby repressing pyroptosis. The regulatory role of AMPK-mediated GSDME phosphorylation was further confirmed in AMPK knockout and GSDME T6E or GSDME T6A knock-in mice. In mouse primary cancer models, mannose administration suppressed pyroptosis in small intestine and kidney to alleviate cisplatin- or oxaliplatin-induced tissue toxicity without impairing antitumor effects. The protective effect of mannose was also verified in a small group of patients with gastrointestinal cancer who received normal chemotherapy. Our study reveals a novel mechanism whereby mannose antagonizes GSDME-mediated pyroptosis through GlcNAc-6P-mediated activation of AMPK, and suggests the utility of mannose supplementation in alleviating chemotherapy-induced side effects in clinic applications.
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JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 61
·期 4
·346-354
·
IF 3.046
Die Li,Mei Hong,Yan Wang,Chunyang Bao,Xiaoling Xu,Jing Chen,Bin Peng,Jingyu Zhang,Bingyu Wang,Qiang Zhang
Porous carbon nanospheres have attracted extensive attention due to their excellent advantages in specific surface area, pore distribution, and electronic conductivity. Herein, boron-doped hollow carbon nanospheres with mesoporous walls have been successfully synthesized via a combination of hard/soft templating and boronic acid/sugar ligation. Surface-initiated Cu(0) mediated reversible deactivation radical polymerization was used to initiate the polymerization of 2-hydroxyethyl acrylate and poly(ethylene glycol) methyl ether acrylate from the surface of silica. 4-carboxy-3-fluorophenylboronic acid was introduced as a boron precursor by N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride coupling and fructose was introduced as a carbon precursor by boronic esters. After carbonization and template etching, boron-doped porous carbon spheres could be obtained. We believe such in-situ heteroatom-doped method has great potential in preparing hollow mesoporous carbon nanospheres with excellent properties. Graphical
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ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Yu Peng,Luyao Jiang,Junhua Di,Cuiluan Ma,Qi Li,Yucai He
Biobased 2,5-bis(hydroxymethyl)furan is a versatile building block for the production of drugs, polymers, crown ethers, bioactive compounds, and value-added intermediates. To explore a more efficient route to synthesize 2,5-bis(hydroxymethyl)furan, a tandem transformation by quick dehydration with a deep eutectic solvent betaine:benzenesulfonic acid (Bet:BSA) and mild bioreduction with recombinant Escherichia coli (E. coli) DCF was developed for converting d-fructose into 2,5-bis(hydroxymethyl)furan. The 5-hydroxymethylfurfural yield reached 62.3 ± 1.6% (based on d-fructose) in Bet:BSA at 120 °C after a short time (2 min). d-Fructose-valorized 5-hydroxymethylfurfural was biologically transformed to 2,5-bis(hydroxymethyl)furan by newly constructed recombinant E. coli DCF containing reductase and formate dehydrogenase at 40 °C and pH 7.5 after 48 h, with a yield of 99.6 ± 0.1% (based on 5-hydroxymethylfurfural) in Bet:BSA-water containing the cosubstrate HCOONa (5-HMF-to-HCOONa molar ratio, 1:2.5). Meanwhile, 150 mM commercial 5-hydroxymethylfurfural was transformed into 2,5-bis(hydroxymethyl)furan in a yield of 94.4 ± 0.9%. An efficient hybrid process was constructed to valorize d-fructose to 2,5-bis(hydroxymethyl)furan in Bet:BSA-water, which has good industrial application potential.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Chunhua Li,Xiao Xu,Fuli Wang,Yue Zhao,Yubo Shi,Xin Zhao,Jue Liu
It is significant to develop low-cost, sensitive, and portable sensing platform for point-of-care testing of ascorbic acid (AA) in food quality evaluation. Herein, a smart point-of-care (SPOC) sensor, consisting of fluorescent paper chip, 3D printed-accessories and smartphone, was developed for ultrasensitive and visual quantitative detection of AA. Fluorescent paper chip was made through printing silicon doped carbon dots (SiCDs)-Fe 3+ as “ink” onto filter paper, in which SiCDs emitting strong fluorescence signal quenched by Fe 3+ , and then recovered by AA owing to releasing –NH 2 /–OH and introducing defects on SiCDs. The smartphone with an application named “ AA-Tester ” was established to analyze AA and exhibited high sensitivity with low detection limits of 18.12 nmol/L. HPLC method was used to verify the SPOC sensor and obtained satisfactory accuracy with relative standard deviations of 0.79–2.31 %. The portable sensing platform integrated fluorescence sensor with smartphone-device will be favorable for ultrasensitive and on-the-spot detection of AA.
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