Food Chemistry-X
·
2022
·
IF 6.443
Qingxia Yuan,Kunling Lv,Jinwen Huang,Shujing Sun,Ziyu Fang,Hongjie Tan,Hong Li,Dan Chen,Longyan Zhao,Chenghai Gao,Yonghong Liu
Grey mangrove ( Avicennia marina (Forssk.) Vierh.) fruit is a traditional folk medicine and health food consumed in many countries. In this study, its polysaccharides (AMFPs) were obtained and analyzed by chemical and instrumental methods, with the results indicating that AMFPs consisted of galactose, galacturonic acid, arabinose, and rhamnose in a molar ratio of 4.99:3.15:5.38:1.15. The dynamic changes in AMFPs during the digestion and fecal fermentation processes were then investigated. The results confirmed that AMFPs were not depolymerized by gastric acid and various digestive enzymes. During fermentation, 56.05 % of the AMFPs were utilized by gut microbiota. Galacturonic acid, galactose, and arabinose from AMFPs, were mostly consumed by gut microbiota. AMFPs obviously decreased harmful bacteria and increased some beneficial microbiota, including Megasphaera , Mistuokella , Prevotella , and Megamonas . Furthermore, AMFPs obviously increased the levels of various short-chain fatty acids. These findings suggest that AMFPs have potential prebiotic applications for improving gut health.
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高影响因子
PROCESS BIOCHEMISTRY
·
2022
·
IF 4.885
Zhengyu Hu,Ruiying Yu,Jinfeng Sun,Yuanqi Duan,Hongli Zhou,Wei Zhou,Gao Li
In this study, polysaccharides from the leaves of Rhododendron dauricum L. (RDPs) were decolorized using the static macroporous resin adsorption method. The RDPs were characterized and their antioxidant activities were investigated. An analytical hierarchy process (AHP) and a response surface method (RSM) were used to determine the optimum decolorization conditions as: a polysaccharide concentration of 8 mg/mL, decolorization time of 70 min, liquid–solid ratio of 6 mL/g, and decolorization temperature of 52 °C, with D101 as the best resin. Furthermore, two novel polysaccharides (RDP-1 and RDP-2) were isolated from R. dauricum leaves and characterized, which demonstrated that RDP-1 and RDP-2 were both composed of mannose , glucose, galactose , and arabinose in different molar ratios. These RDPs also exhibited a triple helix conformation, and primarily contained →6)-Glc p -(1→, →5)-Ara f -(1→, →2,4)-Man p -(1→, →6)-Gal p -(1→ and Glc p -(1→ residues with different connection sequences. Biological activity tests showed that the two polysaccharides possessed scavenging capacity against 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radicals. The results indicated that the antioxidant activities of the RDPs obtained by the static decolorization method improved, which lays a theoretical foundation for developing techniques for utilising R. dauricum .
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Frontiers in Nutrition
·
2022
·
IF 6.59
Hong Tao,Zhao Jiaying,Yin Junyi,Nie Shaoping,Xie Mingyong
Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.
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高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 9
·1389
·
IF 5.719
Jiahang Yu,Jun Qi,Zhen Li,Huixin Tian,Xinglian Xu
Monitoring food quality throughout the food supply chain is critical to ensuring global food safety and minimizing food losses. Here we find that simply by mixing an aqueous solution of sugar-stabilized Ag+and amines in an open vessel leads to the generation of Ag NPs and an intelligent evaluation system based on a colorimetric Ag+probe is developed for real-time visual monitoring of food freshness. The self-assembly reaction between methylamine (MA) generated during meat storage and the colorimetric Ag+probe produces different color changes that indicate changes in the quality of the meat. The colorimetric Ag+probe was integrated into food packaging systems for real-time monitoring of chilled broiler meat freshness. The proposed evaluation system provides a versatile approach for detecting biogenic amines and monitoring chilled broiler meat freshness and it has the advantages of high selectivity, real-time and on-site measurements, sensitivity, economy, and safety and holds great public health significance.Keywords:colorimetric Ag+probe;self-assembly;Ag NPs;amines;food detection
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Lei Li,Yun Fang,Yongmei Xia,Chunling Bo,Ye Fan
Acted as the second component of fatty acid vesicles (FAV), almost all reported chemicals except for bio-incompatible cationic amphiphiles caused a one-side pH window extension of FAV whilst the other-side shrinkage accordingly. We recently found that dodecanol could partially compensate the alkaline-side pH window shrinkage of FAV when the acidic-side was extended by sodium dodecylsulfate (SDS). Therefore, we guess that if one single small molecule played two − in − one function as both SDS and dodecanol done on the different-side pH window, it might cause a two-side pH window extension of FAV entering into near-neutral pH. Taking conjugated linoleic acid (CLA) as the model fatty acid, ribose, glucose, glucosamine and acetyl glucosamine as the models of the second component, the influence on the pH window extension for the FAV of CLA by each of the monosaccharides and the involved noncovalent bonding interactions are investigated by laser Tyndall effect, pH titrimetry, transmission electron microscopy, dynamic light scattering, ultraviolet absorbency, isothermal titration calorimetry measurement and binding energy calculation. The two-side pH window extension for the FAV of CLA is realized by aid of the two − in − one function of monosaccharides that create H-bonding interaction with the FAV surface and thus enhancing hydrophobic self-assembly of CLA. It is crucial for self-assembling FAV as primitive cell membranes and applying it as modern nutrient carriers or drug delivery systems in the physiological pH range. We propose a practical microenvironmental polarity criterion based on the experimental results to predict how the second component to extend the pH window of FAV: If the second component made the FAV surface more hydrophobic on the alkaline-side pH window while more hydrophilic on the acidic-side via molecular interactions, it could drive the corresponding-side pH window extension, otherwise the corresponding-side pH window shrinkage.
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高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 3
·177
·
IF 6.085
Ying Wang,Xiao-Ming Li,Sui-Qun Yang,Fan-Zhong Zhang,Bin-Gui Wang,Hong-Lei Li,Ling-Hong Meng
An unusual sesquiterpene glycoside trichoacorside A (1) and two novel sorbicillinoid glycosides sorbicillisides A (2) and B (3), together with a known compound sorbicillin (4), were isolated and identified from the culture extract of an endophytic fungusTrichoderma longibrachiatumEN-586, obtained from the marine red algaLaurencia obtusa. Trichoacorside A (1) is the first representative of a glucosamine-coupled acorane-type sesquiterpenoid. Their structures were elucidated based on detailed interpretation of NMR and mass spectroscopic data. The absolute configurations were determined by X-ray crystallographic analysis, chemical derivatization, and DP4+ probability analysis. The antimicrobial activities of compounds1–4against several human, aquatic, and plant pathogens were evaluated.Keywords:Trichoderma longibrachiatum;sesquiterpene glycoside;secondary metabolites;antimicrobial activity
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 6
·3385-3399
·
IF 3.612
Yu Zhang,Kexin Li,Maobin Chen,Shangling Fang,Da Zhen,Jinghua Cao,Zhengqi Wu,Ke Zhang
Summary In this study, a novel chrysanthemum polysaccharide (CP) was obtained and investigated from Chrysanthemum morifolium tea in Hubei province, China. Molecular characterisation and emulsifying properties of the CP were elucidated. The effects of different factors, including storage time, metal ions, pH value and heat treatment, were studied on the stability of emulsion. Results showed that the mean particle diameter (MPD) ( d 32 ) varied from 5.54 to 0.49 μm with the polysaccharide concentration ranging from 0.5wt% to 3.0wt%. The MPD ( d 32 ) of emulsions stabilised by chrysanthemum tea polysaccharide (ECP) was smaller than that of GA at 2wt%, and the MPD ( d 32 ) of ECP remained stable with no stratification occurred at 25 °C for 10 days. ECP also showed good stability under acidic conditions (3.0–7.0). With increase of Na + and Ca 2+ concentration, MPD( d 32 ) and zeta potential values raised significantly, from 2.24 to 7.24 μm and from −40.52 to −35.35 mV respectively. After high-temperature heat treatment, MPD ( d 32 ) and zeta potential showed no significant change, indicating ECP presented good storage stability. In conclusion, CP helped stabilised emulsions that could be used as a natural emulsifier for future application.
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高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Hualing Xie,Jingyu Fang,Mohamed A. Farag,Zhenhao Li,Peilong Sun,Ping Shao
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF- α , TGF- β 1, IL-6, IL-1 β ) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides , Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 10
·4167
·
IF 4.6
Shilei Wang,Yuan Peng,Yixin Zhuang,Nan Wang,Jianchang Jin,Zhajun Zhan
Two polysaccharides, named APS2-I and APS3-I, were purified from the water extract of Radix Astragali. The average molecular weight of APS2-I was 1.96 × 106Da and composed of Man, Rha, GlcA, GalA, Glc, Gal, Xyl, and Ara in a molar ratio of 2.3:4.8:1.7:14.0:5.8:11.7:2.8:12.6, while the average molecular weight of APS3-I was 3.91 × 106Da and composed of Rha, GalA, Glc, Gal, and Ara in a molar ratio of 0.8:2.3:0.8:2.3:4.1. Biological evaluation showed APS2-I and APS3-I had significant antioxidant activity and myocardial protection activity. Furthermore, total polysaccharide treatment could significantly enhance hemodynamic parameters and improve cardiac function in rat ischemia and reperfusion isolated heart models. These results provided important information for the clinical application of APS in the field of cardiovascular disease and implied that Astragalus polysaccharides (APS) could be considered as a reference for the quality control of Radix Astragali.Keywords:Astragalus membranaceus;polysaccharide;cardio-protection;antioxidant
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Zhiyong Xie,Yixuan Bai,Guijie Chen,Wei Dong,Yujia Peng,Weiqi Xu,Yi Sun,Xiaoxiong Zeng,Zhonghua Liu
Aspergillus cristatus is the dominant fungus involved in the fermentation of Fuzhuan brick tea (FBT). The intracellular polysaccharides (IPSs) from A. cristatus (MK346334, NCBI), isolated from FBT, exhibited immunomodulatory activity in vitro while the effects in vivo on immune system and gut microbiota remain unclear. In this study, IPSs and the purified fraction (IPSs-2) from IPSs were prepared and their immunomodulatory activities were investigated with cyclophosphamide (Cy)-induced immunosuppressive mice. As results, IPSs strengthened the immune function, manifesting in the improvement of body weight, daily intake, immune organ indices, cytokines and immunoglobulin. Meanwhile, IPSs attenuated Cy-induced intestinal barrier injury and promoted the expression of tight junction proteins and mucin, reinforcing the intestinal barrier function. Moreover, IPSs not only promoted the production of short-chain fatty acids and the expression of G protein-coupled receptor (GPR), but also balanced dysbiosis of gut microbiota through elevating the growth of beneficial bacteria while reducing pathobionts to maintain the homeostasis of the microbial ecology. These results suggested that IPSs exerted immunomodulatory activity linking with the restoration of intestinal barrier function and regulation of gut microbiota, which contributes to the development of novel probiotics and effective immunomodulators for strengthening host immunity and gut health.
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高影响因子
Small
·
2021
·Vol. 18
·期 2
·2104477
·
IF 13.281
Lei Xie,Jinrong Liu,Xiaobing Bao,Jiadong Chen,Xiaozhong Zheng,Yanjun He,Wei Zhang,Jie Zeng,Yong Wang,Biao Kong
Synthesis of anisotropic carbonaceous nano- and micro-materials with well-ordered mesoporous structures has attracted increasing attention for a broad scope of applications. Although hard-templating method has been widely employed, overcoming the viscous forces to prepare anisotropic mesoporous materials is particularly challenging via the universal soft-templating method, especially from sustainable biomass as a carbon resource. Herein, the synthesis of biomass-derived nanowire-arrays based mesoporous nanorods and teeth-like superstructures is reported, through a simple and straightforward polyelectrolyte assisted soft-templating hydrothermal carbonization (HTC) approach. A surface energy induced interfacial assembly mechanism with the synergetic interactions between micelles, nanowire, nanorods, and polyelectrolyte is proposed. The polyelectrolyte acts not only as a stabilizer to decrease the surface energy of cylindrical micelles, nanowires and nanorods, but also as a structure-directing agent to regulate the oriented attachment and anisotropic assembly of micelles, nanowires, and nanorods. After a calcination treatment, the carbon nanorod and teeth-like superstructure are successfully coupled with Ru to directly produce supported catalysts for the hydrogen evolution reaction, exhibiting much better performance than the isotropic nanospheres based catalyst. This HTC approach will open up new avenues for the synthesis of anisotropic materials with various morphologies and dimensions, expanding the palette of materials selection for many applications.
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Shuying Zhao,Shibing Wang,Chenglong Qiu,Zaixiang Xu,Linhan Ren,Yuhang Wang,Suiqin Li,Jiahui He,Kai Li,Yinjie Xu,Mengxin Wang,Xing Zhong,Jianguo Wang
Indirect electrocatalytic alcohol oxidation mediated by aminoxyl radicals generally requires large amounts of aminoxyl radicals to activate the oxidation of alcohols, which limits its potential for industrial utilization. In this study, the cavity structure of carbonaceous nano-volcanoes (CNV) grown on 3D graphite felt as nanoreactors advantageously provides a microenvironment with high compartmentalised reactant concentrations for efficient electrocatalytic alcohol oxidation. By extending the retention time of reactant molecules in the CNV through cavity enrichment, their interaction probability with the electrodes was maximized, resulting in the intensification of mass transport and kinetically favorable settings. Meanwhile, abundant oxygen-containing functional groups on the surface constructed by the CNV provided hydrogen bonding forces to anchor the reactive aminoxyl radicals (4-acetamido-2,2,6,6-tetrame-thylpi-peridin-1-oxyl, ACT) during the reaction. Based on the intermediate-rich local microenvironment, a high turnover frequency of up to 31818 h −1 can be achieved even with only 0.5 mol% ACT-mediated. The electrocatalytic oxidation of benzyl and heterocyclic substrates can be achieved by this method under mild conditions. To showcase the synthetic efficacy and practical applicability of this approach, the successful oxidation of a chiral primary alcohol precursor to the generic drug levetiracetam was demonstrated on a larger scale, presenting further evidence of its practicality and usefulness in chemical synthesis.
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高影响因子
危险品化学品经营许可证（带存储）