Science China-Chemistry
·
2024
·
IF 10.4
Xu Xing,Zeng Xi,Lin Xin,Lin Shiyan,Liang Shanshan,Tian Tian,Su Rui,Song Jia,Yang Chaoyong
Single-cell joint analysis of methylome and transcriptome reveals how the methylation regulates the transcriptional activity. However, traditional bench-top protocols for single-cell DNA methylation and RNA transcription co-detection are labor-intensive, cost-ineffective and contaminant-prone. Herein, we establish the DMF-scMT-seq, a highly-efficient and cost-effective method to simultaneously analyze single-cell DNA methylation and transcriptional activity based on digital microfluidics. DMF-scMT-seq automates the workflow of single-cell isolation, cellular hypotonic lysis, nucleic acid separation and methylome/transcriptome library construction in a contactless and addressable way. The system ensures high accuracy ( R >0.85), high gene detection ability (14,697 genes per cell at 4 million sequencing depth), and high CpG coverage (677,198 CpG sites per cell at 1 million sequencing depth). By using DMF-scMT-seq, the relationship of DNA methylation and RNA transcription under different genomic contexts is resolved. We further apply DMF-scMT-seq to study the dynamics of transcription regulation with methylation-inhibiting anti-tumor Decitabine, and identify the methylated promoter/gene body driven genes in response to Decitabine treatment. DMF-scMT-seq facilitates the construction of the correlation of DNA methylation and transcriptional activity at the single-cell level in a flexible, sensitive and accurate way, which is anticipated to be a powerful tool in studying single-cell biological systems.
研究领域:
生物信息学
·
表观遗传学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Wenzhong Ma,Taiyu Li,Chao Jiang,Peng Zhang,Li Deng,Rong Xu,Qi Zhang,Jing Zhong,Hideto Matsuyama
Pervaporation (PV) performance of polyimide (PI) membranes has been attempted for the recovery of high concentrations of aprotic solvents, such as N, N-dimethyl formamide (DMF) and N, N-dimethylacetamide (DMAc). In this work, we explored the effect of chain structure on the solvent resistance and pervaporation performance of hydrophilic pyromellitic dianhydride (PMDA) and 3,3′,4,4′-benzophenonetetracarboxylic dianhydride (BTDA) based polyimide membranes. According to the Hansen solubility parameters and membrane formation morphologies, we selected five aprotic solvent resistant PI membranes with different chain structures. It was found that the swelling degrees in DMF and DMAc was principally determined by the elasticity modulus of the PI membrane, and was significantly influenced by the polymer chain structure. The flexible linkages (such as –O CH 2 and CH 2 ) suppressed the PI chain packing, which resulted in the d -spacing and fractional free volume ( FFV ) of PMDA based PI membrane was higher than BTDA based PI membrane. BTDA-BZD membrane had the highest elasticity modulus together with the lowest d -spacing value (4.68 Å) due to its symmetric intermolecular interaction and rigid chain structure. PV experiments were conducted with DMF/water and DMAc/water mixtures over a wide range of temperatures (30–60 °C) with 90 wt% solvent concentration. The BTDA based PI (M4) composite membrane showed the highest separating factor around 275 at 30 °C in DMAc solution, which was much higher than in DMF solution (the separating factor is about 70). Furthermore, the chemical and thermal stability test for the PI composite membranes in the presence of the feed solvent under the PV process demonstrated the excellent separation stability in DMAc solution.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Yongwei Chen,Houxiao Wu,Zewei Liu,Xuejiao Sun,Qibin Xia,Zhong Li
Mechanochemical synthesis, driven the chemical transformation by mechanical force, has been a rapid and efficient strategy to prepare metal–organic frameworks (MOFs). In this work, a liquid-assisted mechanochemical method has been successfully applied to the synthesis of copper based MOF-505. The crystallinity and porosity of MOF-505 were investigated by adjusting the synthesis parameters, such as added solvent (type and amount) and grinding time. Results showed that the type and amount of added solvent were crucial parameters for the mechanochemical synthesis of MOF-505. Furthermore, the optimizations of MOF-505 synthesis were carried out: grinding Cu(OAc)2·H2O and H4bptc as starting materials for 80 min with 0.4 mL of DMF assisted, giving MOF-505-K with Brunauer–Emmett–Teller (BET) surface area of 977 m2/g. Meanwhile, MOF-505-K had moderate CO2 adsorption capacity of 2.01 mmol/g at 298 K and 100 kPa. The adsorptive selectivity determined from ideal adsorbed solution theory (IAST) indicated that MOF-505-K had high CO2/N2 and CO2/CH4 selectivities (26.6 and 5.5) at 298 K. Highly efficient mechanochemical synthesis of MOF-505 provides the promise to facilitate the industrial application of CO2 capture.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 61
·35524-35531
·
IF 3.049
Xinyu Mao,Liqun Zhu,Huicong Liu,Haining Chen,Pengfei Ju,Weiping Li
Directly dispersing graphene into an electrolyte still remains a crucial difficulty in electrodepositing a graphene enhanced composite coating onto electrical contact materials. Herein, graphene was synthesized via electrochemical exfoliation in an N,N-dimethylformamide (DMF)/H2O solution containing (NH4)2SO4. The electrochemically exfoliated graphene nanosheets (GNs) were directly dispersed by sonication. In comparison with graphene synthesized from aqueous solution, the GNs electrochemically exfoliated in the DMF/H2O–(NH4)2SO4 solution exhibit a lower degree of oxidation. Cu/graphene composite coatings were subsequently electrodeposited onto Cu foils by adding Cu2+ into the as-fabricated graphene solution. The surface nanostructure of the Cu/graphene composite coatings was transformed from loose pine needles to a uniform and compact structure with an increase in the concentration of Cu2+, which indicated that the controllable synthesis of Cu/graphene composite coatings with different performances could be achieved in graphene dispersions after adding Cu2+. In order to synthesize graphene via electrochemical exfoliation and directly electrodeposit a Cu/graphene composite coating without adding CuSO4 or any other additive, an attempt was made to directly electrodeposit a Cu/graphene composite coating in CuSO4/DMF/H2O solution after electrochemical exfoliation.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RARE METALS
·
2024
·Vol. 43
·期 8
·3748-3757
·
IF 9.6
Ye Xiang-Yang,Bao Ke-Pan,Luo Sai-Nan,Li Xin,Chen Tai-Qiang,Xia Shui-Xin,Yuan Tao,Pang Yue-Peng,Zheng Shi-You
Composite solid electrolytes (CSEs) containing polymer matrices and inorganic fillers hold promise for the next generation of solid-state batteries. However, the role of residual solvents in CSEs remains controversial. This study investigated the evolution and function of the residual solvent in a polymer-Li 2 B 12 H 12 CSE. A partial reaction occurred between Li 2 B 12 H 12 and solvent N, N-dimethylformamide (DMF), which produced dimethylaminomethanol (DMAM) in the CSE. Density functional theory calculations have revealed that DMAM forms stronger hydrogen bonds with polyvinylidene fluoride chains than DMF, which can have a plasticizing effect on the polymer matrix, leading to lower crystallinity and quicker segment motion. Therefore, this CSE exhibited improved Li-ion conducting properties, enabling the stable cycling of Li||LiFePO 4 solid-state batteries. This study provided insights into the role of residual solvents in CSEs.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yujie Chen,Caiyun Zhang,Jiangyang Xie,Haijie Li,Wenjing Dai,Qiliang Deng,Shuo Wang
A highly crystalline covalent organic frameworks (COFs) formed by condensation reaction between 1, 3, 5-tris (4-aminophenyl) benzene and 4, 4′-biphenyldicarboxaldehyde is utilized as a sensing platform for water in organic solvent over a broad concentration range. The resulting COFs exhibits brilliant fluorescence in various organic solvents such as methanol, DMF, acetonitrile and ethanol, moreover its fluorescence intensity has a significant and rapid response to the content of water in organic solvent over a broad concentration range. The broadest sensing range is achieved over 7%–70% (v/v) for water in DMF, and the lowest limit of detection is 0.042% (v/v) for water in methanol among the investigated organic solvents. The superior properties of the sensing platform expand the application ranges of COFs and endow the resulting COFs with a great prospect in practical applications for highly efficient detecting water in organic solvent.
研究领域:
材料科学
·
化学传感
✓ 已验证
DOI
高影响因子
Polymers
·
2018
·Vol. 10
·期 3
·289
·
IF 2.935
Yulu Wang,Liqiang Jin
A novel self-colored waterborne polyurethane (SCPU) was synthesized and used in the preparation of a microfiber synthetic leather (MS-Leather) base in order to reduce the pollution caused byN,N-dimethylformamide (DMF) and dyes. The SCPU was prepared using the reaction of a reactive brilliant red K-2G with a waterborne polyurethane which was then extended via a first generation of hyperbranched poly(amine-ester). With the introduction of the dye, new absorption peaks at 1118 cm−1[S=O], 1413 cm−1[N=N], and 1635 cm−1[C=N] appeared in the Fourier transform infrared (FTIR) spectrum of SCPU, and SCPU mean particle size increased to 162 nm. The X-ray diffraction (XRD) peak intensity of SCPU at 19.27° was 1310 cts. The thermal stability of SCPU at 200–280 °C was inferior to that of the control sample; however, it improved at temperatures above 360 °C. Finally, a free-dyeing MS-Leather base prepared by using SCPU without DMF was manufactured. It was found that the dyes were distributed mainly in the polyurethane matrix rather than in the microfibers. Moreover, the color changes of the base before and after being washed in both a water and a soap solution were 0.93 and 4.21, respectively. This indicated that the base’s washing color fastness to water was better than to a soap solution.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2011
·Vol. 49
·期 22
·4721-4724
·
IF 3.894
Hou Chen,Min Zhang,Mengmeng Yu,Hongyan Jiang
Graphical Iron tube was applied as catalyst source in the design of continuous SET-LRP of AN without any ligand using BPN as initiator in NMP and DMF, respectively. First-order kinetics of polymerization with respect to the monomer concentration, linear increase of the molecular weight, and narrow polydispersity with monomer conversion showed the controlled radical polymerization characters. An increase in polymerization rate in NMP was observed compared with in DMF. The living feature of PAN was verified by chain extensions of PAN with MMA.
研究领域:
化学工程
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
OPTICAL MATERIALS
·
2021
·
IF 3.08
Yunpeng Wang,Ruiting Hao,Jie Guo,Xiaoming Li,Shuiliu Fang,Huimin Liu,Shuaihui Sun
In this paper, magnesium was successfully introduced into CZTS using the DMF-based solution method, and CZMTS solar cell was prepared. The effects of Mg doping on the morphology, crystal structure, and electronic properties of the CZTS absorber were investigated in detail. The crystal structure of the CZMTS absorber and the chemical valence states of the other elements (Cu, Zn, Sn, and S) were not changed as shown by XRD , Raman, and XPS analyses. The results show that Mg doping can improve the electronic properties and microstructure of CZTS absorbers, thus enhancing the PCE from 2.91% to 4.10%. This performance enhancement is associated with good crystal quality, reduced cation-disordering defects, and high external quantum efficiency at the longwave region caused less recombination and open circuit voltage loss. Therefore, Mg doping has a positive effect on improving the performance of CZTS solar cells.
研究领域:
材料科学
·
能源科学
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Yong Zhang,Jingshu Tang,Yujun Zhou,Qiong Xiao,Qiuyu Chen,Hongyue Wang,Jiaqi Lan,Lei Wu,Ying Peng
Background: The pharmacological activity of dimethyl fumarate (DMF) in treating psoriasis and multiple sclerosis (MS) is not fully understood. DMF is hydrolysed to monomethyl fumarate (MMF) in vivo , which is believed to account for the therapeutic effects of DMF. However, previous studies have provided evidence that DMF also enters the circulation. Given that DMF is short-lived in the blood, whether DMF has a therapeutic impact is still unclear. Methods: Lipopolysaccharide (LPS)-mediated RAW264.7 cell activation was used as a model of inflammation to explore the anti-inflammatory effects of short-term DMF exposure in vitro . Whole blood LPS stimulation assay was applied to compare the anti-inflammatory effects of DMF and MMF in vivo . Griess assay was performed to examined nitrite release. The expression of pro-inflammatory cytokines and transcription factors were measured by quantitative PCR (qPCR), ELISA and Western blot. Depletion of intracellular glutathione (GSH) was evaluated by Ellman’s assay. Luciferase reporter assays were performed to evaluate DMF effects on Nrf2-ARE pathway activation, promoter activity of Nfkbiz and mRNA stability of Nfkbiz . Binding of STAT3 to the IκBζ promoter were examined using Chromatin immunoprecipitation (ChIP) assay. Results: Short-term exposure to DMF significantly inhibited the inflammatory response of RAW264.7 cells and suppressed LPS-induced IκBζ expression. Importantly, oral DMF but not oral MMF administration significantly inhibited IκBζ transcription in murine peripheral blood cells. We demonstrated that the expression of IκBζ is affected by the availability of intracellular GSH and regulated by the transcription factor Nrf2 and STAT3. DMF with strong electrophilicity can rapidly deplete intracellular GSH, activate the Nrf2-ARE pathway, and inhibit the binding of STAT3 to the IκBζ promoter, thereby suppressing IκBζ expression in macrophages. Conclusion: These results demonstrate the rapid anti-inflammatory effects of DMF in macrophages, providing evidence to support the direct anti-inflammatory activity of DMF.
研究领域:
药理学
·
免疫学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2026
·Vol. 237
·期 21
·1249
·
IF 3.5
Liu Guowei,Liu Baolong,Peng Xiaoqian,Gao Ning,Wang Sheng,Yuan Zhongshan,Chen Yingwen,Fan Mengjie
High-concentration N,N-dimethylformamide (DMF) wastewater presents a major challenge in hazardous chemical waste treatment due to its chemical stability, poor biodegradability, and significant biotoxicity. To address the insufficient thermal stability of conventional catalyst supports, limited activity of single metals, and the lack of synergistic catalyst–reactor process studies in thermocatalytic oxidation, this thesis focuses on multiphase fluidized-bed catalytic cracking and establishes an integrated “catalyst–reactor–process” technical system. Transition metal-doped alumina aerogel catalysts were prepared via a sol–gel method, with Ni and Cu identified as the optimal active components. The optimized Ni 3 Cu 2 O x –Al 2 O 3 catalyst (Ni/Cu oxide mass ratio of 3:2, calcined at 550 °C for 5 h) achieved 99.96% DMF cracking and 98.66% chemical oxygen demand (COD) removal for 8000 mg/L DMF at 380 °C. Characterization revealed a three-dimensional mesoporous structure with a specific surface area of 236.44 m 2 /g. The fluidized-bed operating parameters were systematically optimized, and good catalytic performance was obtained over a DMF concentration range of 8000–32000 mg/L. No apparent competitive inhibition was observed in DMF–phenol mixed wastewater, demonstrating the general applicability of the catalytic process. The constructed fluidized-bed/fixed-bed two-stage series process achieved deep purification, with DMF cracking approaching 100% and a maximum COD removal of 99.33%. This study provides an efficient catalytic process scheme for the harmless treatment of high-concentration DMF wastewater. Graphical
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Lingbo Liu,Jian Shen,Kangbing Wu,Nianjun Yang
Commercially available and stable red phosphorus (RP) is a promising material to produce two-dimensional RP nanomaterials. In this work, RP nanosheets are produced using a liquid-phase exfoliation approach with aid of ultrasonication. The RP nanosheets exfoliated in the solvents of ultrapure water, isopropanol, and N,N-dimethylformamide (DMF) exhibit different sizes and thicknesses. More uniform and thinner RP nanosheets are obtained in DMF (ERP-DMF), due to optimized surface energies between RP nanosheets and DMF. These RP nanosheets show varied activities towards electrooxidation of one case molecule of 4-nitrophenol (4-NP). ERP-DMF features a larger active area and a higher electron transfer rate than those produced in water and isopropanol. Sensitive, reproducible, stable, and selective monitoring of 4-NP is then achieved using ERP-DMF as the sensing material. This study provides a general approach to produce two-dimensional nanomaterials from bulk materials as well as the guidance to explore their potential applications.
研究领域:
材料科学
·
电化学
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）