MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Manlin Luo,Chengyan Yin,Wei Liu,Kerage Dorothy Mokeira,Meng Luo,Ling Tang,Jing Liu,Shuxia Xu
In this study, a Ce13d DNAzyme-based electrochemical biosensor for the detection of sodium ions (Na + ) in the serum was developed. The indium tin oxide (ITO) electrode was modified with Au gold nanoparticles (AuNPs), then immobilized with double-stranded DNA formed by thiol of substrate strand and enzyme strand bound via Au-S link. In the presence of Na + , the resulted Ce13d DNAzyme could preferentially bind Na + , and induced local folding of Ce13d to form a G-quadruplex-like structure, which enhanced binding ability to methylene blue (MB). By using the specificity of DNAzyme and change of its conformation, the quantitative detection of Na + could be achieved. Thus, the developed sensing platform could identify Na + with a linear response range of 10 nM-100 mM and a detection limit of 1.03 nM. The electrochemical biosensor also exhibited excellent selectivity for Na + in the presence of five interfering ions, including K + , Cu 2+ , Mg 2+ , Zn 2+ and Ca 2+ . Furthermore, the proposed biosensor showed satisfactory results for Na + in serum sample analysis, which highly correlated with the inductively coupled plasma atomic emission spectrometry method. This work demonstrated a simple and reliable method for Na + in serum, which opens the way for potential applications in disease diagnosis and environmental testing.
研究领域:
生物化学
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医学检测
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分析化学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2017
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IF 3.012
Hai-Yan Song,Tian-Fang Kang,Meng-Fan Jiang,Jing-Jing Zhang,Shui-Yuan Cheng
A novel, simple, and versatile electrochemiluminescence (ECL) sensing platform for Pb(II) ions was developed based on DNAzyme with peroxidase-like activity to catalyze the reduction of hydrogen peroxide. Poly (diallyldimethylammonium chloride) (PDDA) with positive charge was adsorbed onto the surface of graphene oxide (GO) and then CdS quantum dots (QDs) with negative charge were enriched to the surface of GO by electrostatic interaction between CdS QDs and PDDA to prepare a composite (denoted as [email protected] ) which was immobilized on the surface of a glassy carbon electrode (GCE). When hydrogen peroxide was used as coreactant CdS QDs can produce ECL signal. G-rich DNA strand, i . e . T30695, can combine with Pb (II) ions to form a stable parallel G-quadruplex. The G-quadruplex can further combine with hemin to form DNAzyme which have catalysis towards the reduction of hydrogen peroxide causing the decrease of ECL signal. Amino-modified T30695 was attached to [email protected] composite by the reaction between the amino group of T30695 and carboxyl group of CdS QDs. The ECL signal of the biosensor was linearly dependent on the logarithm of Pb(II) concentration from 1.0 × 10 − 14 to 1.0 × 10 − 11 M with a detection limit of 9 fM. An excellent performance of ECL biosensor indicated that it is promising for Pb(II) detection in real samples.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2017
·
IF 6.32
Linlin Liang,Feifei Lan,Shenguang Ge,Jinghua Yu,Na Ren,Mei Yan
A novel metal-enhanced ratiometric fluorescence/naked eye bimodal biosensor based on ZnFe2O4@Au–Ag bifunctional nanocomposite and DNA/CeO2 complex for lead ions (Pb2+) has been successfully developed. The nanocomposite probe was composed of a magnetic ZnFe2O4 core and a Au–Ag hollow nanocube shell. Upon bioconjugation, bifunctional magnetic nanocomposites could not only make the probe possess excellent recyclability but also provide an enrichment of “hot spots” for surface enhanced fluorescence detection of Pb2+ by a metal-enhanced fluorescence effect. Typically, the bifunctional nanocomposites conjugated with double-stranded DNA (included Pb2+-specific DNAzyme strand and corresponding substrate strand) to form a Pb2+ biosensor. Nanoceria as a fluorescence quencher strongly adsorbed DNA. Therefore, the formation of double-stranded DNA brought the labeled nitrogen sulfur doped carbon dots (N,S-CDs) and CeO2 into close proximity, which significantly quenched the fluorescence of N,S-CDs. The presence of Pb2+ led to the breakage of the DNAzyme strand, resulting in the fluorescence signal of Cy3 decreasing, while the fluorescence intensity of N,S-CDs aggrandized. First, a preliminary test of Pb2+ was performed by the naked eye. The disengaged DNA/CeO2 complex could result in color change after adding H2O2 because of autocatalysis of CeO2, resulting in real-time visual detection of Pb2+. If further accurate determination was required, the fluorescence intensity ratio of these two fluorescence indicators was measured at 562 and 424 nm (I562/I424). A good linear correlation exists between the log(I562/I424) and the logarithm of Pb2+ concentrations ranging from 10–12 to 3 × 10–6 M. Remarkably, the detection limit of this ratiometric biosensor was 3 × 10–13 M, which ascribed to its superior fluorescence enhancement. Interestingly, the developed bifunctional nanocomposite probe manifests good recyclability and selectivity. More importantly, the biosensor provided potential application of on-site and real-time unknown Pb2+ ions in real systems.
研究领域:
生物传感
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Catalysts
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2022
·Vol. 13
·期 1
·81
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IF 4.501
Ying Zhang,Junya Liao,Hao Liang
DNA-based biomimetic enzymes have attracted extensive attention due to their unique structure and stability compared to natural enzymes. Meanwhile, the specific sequences of DNA itself also have a catalytic effect. Herein, we first designed three guanine-rich DNA sequences numbered c−Myc3c, PG4TC, and TTT to construct g−quadruplex/hemin DNAzymes. Then, the g−quadruplex/hemin DNAzymes with the best activity were selected by a comprehensive examination of activity, degradation rate, and affinity. Subsequently, the stability and reusability of UiO66−DNAzymes were investigated using UiO66 as the carrier to immobilize DNAzymes. The results showed that UiO66−DNAzymes had excellent reusability and stability. Finally, UiO66−DNAzymes were successfully used for glucose detection by cascading with glucose oxidase (GOx) with a detection limit of 0.62 μM. The constructed glucose sensor had a good specificity, which is of great significance for developing a novel, accurate, fast, and economical glucose detection sensor.Keywords:metal-organic frameworks;DNAzymes;enzyme immobilization;biomimetic enzymes;glucose detection
研究领域:
生物化学
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纳米技术
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分析化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Xiuhua Zhang,Heqin Liu,Wanrong Hong,Yumin Wang,Jin-Wen Liu,Yingfeng Qin
In this work, a novel fluorescence nanoprobe based on MnO 2 nanosheets and DNAzyme cyclic amplification was developed for label-free detection of GSH. In our design, the DNA@MnO 2 nanoprobe was facilely prepared through the adsorption of DNA probes on the surface of MnO 2 nanosheets. Importantly, MnO 2 nanosheets in the DNA@MnO 2 nanoprobe can act as the recognizer of analytical target GSH. In the presence of GSH, the DNA@MnO 2 nanoprobe are decomposed owing to redox-responsive reaction between GSH and MnO 2 nanosheets, resulting in the generation of abundant Mn 2+ and the desorption of DNA probes. Subsequently, the Mn 2+ -dependent DNAzyme recycling amplification was rapidly initiated and a large number of the blocked G-rich sequences in DNA probes was released. The G-rich sequences were efficiently folded into G-quadruplex structures, which can remarkably light up the fluorescence intensity of thioflavin T (ThT). Experimental data demonstrated that the DNA@MnO 2 nanoprobes for GSH detection have a wide linear range from 0 to 2000 µM. Moreover, this fluorescence sensing strategy was successfully applied for the detection of GSH in complex biological media with satisfying results, providing a simple, facile and cost-efficient nanoplatform for GSH-related clinical disease diagnosis.
研究领域:
分析化学
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纳米技术
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生物医学工程
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生物化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2014
·
IF 4.517
Xue Gong,Jinfu Li,Wenjiao Zhou,Yun Xiang,Ruo Yuan,Yaqin Chai
Based on target recycling amplification, the development of a new label-free, simple and sensitive colorimetric detection method for ATP by using un-modified aptamers and DNAzymes is described. The association of the model target molecules (ATP) with the corresponding aptamers of the dsDNA probes leads to the release of the G-quadruplex sequences. The ATP-bound aptamers can be further degraded by Exonuclease III to release ATP, which can again bind the aptamers of the dsDNA probes to initiate the target recycling amplification process. Due to this target recycling amplification, the amount of the released G-quadruplex sequences is significantly enhanced. Subsequently, these G-quadruplex sequences bind hemin to form numerous peroxidase mimicking DNAzymes, which cause substantially intensified color change of the probe solution for highly sensitive colorimetric detection of ATP down to the sub-nanomolar (0.33 nM) level. Our method is highly selective toward ATP against other control molecules and can be performed in one single homogeneous solution, which makes our sensing approach hold great potential for sensitive colorimetric detection of other small molecules and proteins.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
AQUACULTURE
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2025
·
IF 3.9
Shuai Zhang,Siying Li,Huili Tian,Ming'ao Wang,Mingsheng Lyu,Shujun Wang
Decapod Iridescent Virus 1 (DIV1) leads to high mortality rates in shrimp farming, significantly impacting the aquaculture industry economically. Currently, there are no effective treatments for controlling DIV1 infections, and management primarily relies on preventative measures. Rapid and precise diagnosis of DIV1 is crucial. In this study, we identified two DNA enzymes, MCP-1 and MCP-8, that can specifically recognize DIV1 via in vitro selection. The detection limit of MCP-1 was 6.89 copies/μL of DIV1. We developed a DNAzyme-based “turn off” biosensor for quick detection of DIV1, which is both highly specific and sensitive, by integrating SYBR Green I to enhance the signal. The detection limit of biosensor could reach 2.01 copies/μL of DIV1. Compared to conventional detection methods, this sensor delivers results in 5 min without the need for complex procedures, greatly streamlining the detection process.
研究领域:
分子生物学
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水产养殖学
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病毒学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
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2024
·
IF 4.1
Xiaona Zhai,Song Yang,Shuai Wang,Pengcheng Wang,Debin Ji
DNAzymes have gained various applications in scientific fields, but their practical uses in everyday life are still being investigated. Hair dyeing, a popular cosmetic practice, still poses potential health risks, owing to the toxicity of commercial hair dyes. In this study, we assessed the feasibility of DNAzyme-catalyzed hair dyeing using natural pigments. We found that DNAzymes of peroxidase activity can expedite dopamine polymerization, resulting in the black coloration of human hair in around half an hour. This process can be achieved by using the milder Tris buffer to substitute the NH 4 OH with 0.1 mM H 2 O 2 . In addition, these DNAzymes can catalyze the polymerization of other natural pigments. Thus, our mild and rapid hair dyeing approach has the potential to substitute traditional harsh hair dyeing conditions and also provides new insights into the field of DNAzyme applications.
研究领域:
生物化学
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化妆品科学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
·
IF 14.224
Jiamin Xu,Mingbin Liu,Weihua Zhao,Suqin Wang,Minfang Gui,Hongbo Li,Ruqin Yu
Lead ions are one of many common environmental pollutants, that can cause posing a serious threat to people’s health, thus, their efficient and sensitive detection is important. We propose a cascade signal amplification sensor using a DNAzyme-based strand displacement amplification (SDA) and hybridization chain reaction (HCR) for the high-sensitivity detection of Pb 2+ . In the demonstrated sensor system, the target metal ion can activate DNAzyme to cause a strand displacement reaction. Under the synergistic action of polymerase and nickase, large numbers of DNA strands are generated that can initiate HCR. The subsequent HCR can restore the fluorescence intensity of the FAM quenched for the fluorescence resonance energy transfer effect, which exhibits a strong fluorescence signal. The DNAzyme-based sensor allowed the detection of Pb 2+ down to 16.8 pM and resulted in a good dynamic line relationship ranging from 50 pM to 500 nM, and the biosensor also showed good selectivity . Furthermore, we confirmed that the proposed sensor can still detect lead ions in complex environments such as lake water, milk, and serum. We believe these findings will provide new ideas for the detection of toxic elements ions in the environment and food.
研究领域:
环境科学
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生物传感
·
分子生物学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Ruiying Li,Qiang Liu,Yan Jin,Baoxin Li
A short G-rich sequence with three G-tracts can form a G-triplex (G3), a recently identified noncanonical DNA structure . Until now, very limited functional study and application of G3 is reported. Herein, we integrated G3 with isothermal exponential amplification reaction (EXPAR) for achieving simple and sensitive biosensing strategy. In this strategy, the cascade EXPAR cycles produces larger numbers of short G-rich sequences, which self-assemble into G3 structure and then bind hemin to form G3/hemin DNAzyme. This G3/hemin DNAzyme–based EXPAR strategy could detect as low as 4.7 fM target DNA with colorimetric detection, and the sensitivity of G3/hemin DNAzyme–based EXPAR strategy was much higher than that of the conventional G-quadruplex/hemin DNAzyme–based EXPAR strategy. We explored the reason for higher sensitivity of G3/hemin DNAzyme–based EXPAR strategy. The experimental results demonstrated that G3/hemin DNAzyme is an ideal signal generator for EXPAR-based biosensing platform. This work opens a new avenue to develop effective signal amplification strategy for ultrasensitive biosensing. This work is also helpful for a deeper understanding of G3 structure and the future application of G3.
研究领域:
分子生物学
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分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Junhua Chen,Gu Shi,Chong Yan
A visual detection method was proposed for polychlorinated biphenyls (PCBs) detection using lateral flow test paper as the sensing platform. The aptamer sequence was used to recognize the target 3,3′,4,4′-tetrachlorobiphenyl (PCB77). The integration of Zn2+-dependent DNAzyme with toehold-mediated strand displacement reaction significantly improved the response signals. Gold nanoparticles were utilized as the signal tracers in the test paper, making the results visible directly by the naked eye. Under optimal conditions, the paper enables the visual detection of PCB77 as low as 10 pM without additional instrumentation. The assay displays a high selectivity for PCB77 against potential interfering molecules. The visual test paper is robust and has been applied to the detection of PCB77 in milk samples with good recovery and satisfactory accuracy. Using two different PCBs (PCB77 and PCB72) as inputs, we further fabricated OR and AND logic gates, which is conducive to the development of an intelligent detection strategy for PCBs monitoring. Given the attractive characteristics of disposability, low cost, logic operation, and intuitive output, the test paper shows great promise for on-site screening of PCBs in resource-limited areas.
研究领域:
分析化学
·
环境科学
·
生物传感
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Yue-Hong Pang,Yi-Ying Wang,Meng-Meng Sun,Xiao-Fang Shen
The cauliflower mosaicvirus 35S (CaMV35S), as the most common promoter, has a usage rate of 70 % in transgenic crops, especially in dicots. Herein, a visual detection method of rolling circle amplification (RCA) combined with DNAzyme technology was developed for the determination of CaMV35S gene. A template sequence of the G-quadruple gene was designed for combining with the target gene CaMV35S. When the target gene existed, the RCA reaction occurred through the action of the enzyme, and the product combined with hemin to generate DNAzyme, which catalyzed the color reaction of 2′-hydrazine-bis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS). Under the optimal experimental conditions, the linear range is 1 × 10 −13 ∼1 × 10 -8 mol/L, and the detection limit is 3 × 10 -14 mol/L (S/N = 3). The method has been successfully applied to standard samples and practical samples (soybean and tofu). For verifying the feasibility and reliability, real-time fluorescent quantitative PCR method was used to detect the target gene CaMV35S, and the results were in good agreement with those of our method, indicating that our developed method can be used for the determination of actual samples.
研究领域:
分子生物学
·
基因工程
·
农业科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）