MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Chunlei Yu,Keyun Ren,Xuezhen Xu,Jinmiao Ma,Falan Li,Yanyan Zhang,Qingqing Yang
Most applications of CRISPR-Cas12a technology for mycotoxin detection rely on aptamers as recognition elements. However, although antibodies exhibit high affinity and specificity, they require converting immune signals into nucleic acid signals and cannot directly activate the CRISPR-Cas12a system, thereby limiting their utility for highly sensitive small-molecule target detection. In this work, we conjugated CRISPR-Cas12a activator DNA (aDNA) to antibodies via strain-promoted azide-alkyne cycloaddition (SPAAC) to achieve dual functionality: transducing small-molecule target signals into nucleic acid signals and partially immobilizing the Cas12a protein. Magnetic spherical nucleic acids (MSNAs) served as trans-cleavage substrates for the CRISPR-Cas12a system. Terminal deoxynucleotidyl transferase (TdT) was used to extend polyA sequences by adding dATP to the 3’-OH ends of numerous cleaved DNA fragments. This process, combined with high-concentration NaCl-induced aggregation and color change of gold nanoparticles (AuNPs), established a cascade signal amplification system. We developed a novel ultrasensitive immunosensor, termed Cas12a-TdT Cascade Amplification for Antigen-Toxin Immunoassay (CTCA-ATI), for mycotoxin quantification. The limits of detection for aflatoxin B1 and deoxynivalenol (DON) were 2.63 × 10 −5 ng/mL and 6.23 × 10 −4 ng/mL, respectively, with linear ranges of 1 × 10 −4 –10 ng/mL for aflatoxin B1 and 1 × 10 −3 –10 ng/mL for DON. This study provides new insights into the development of cascade-amplified biosensors using CRISPR-Cas12a and demonstrates substantial potential for integrating antibodies with CRISPR-Cas technology to detect other critical targets.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Zhilei Zhao,Mengyu Ren,Wenqi Zhang,Zikuo Chen,Lu Zhang,Xinquan Qu,Junling Shi,Weiwei Xia,Xiaoguang Xu,Ying Yang
In view of the serious harm and widespread pollution caused by deoxynivalenol (DON), it is crucial to quantify its presence in the food safety assessment process. Here, we designed a core-shell-satellite nano assembly structure as a probe, composed of an anisotropic AuNR@Ag core, an ultra-thin SiO 2 layer and a high surface coverage AuNPs satellites, which showed outstanding SERS activity and high stability. Anti-DON antibodies were modified on the surface of the prepared core-shell-satellite nano-assembly structure as SERS immunoprobe for DON specific detection using SERS-based lateral flow immunoassay (LFIA) with the advantages of simplicity, rapidity, and high sensitivity. Under optimal conditions, the detection limit for detecting DON using the SERS-LFIA method was 0.053 fg/mL, and the linear detection ranged from 0.1 fg/mL to 1 μg/mL. The SERS-LFIA strips based on AuNR@Ag@SiO 2 -AuNP nanostructures demonstrated the great potential for quick quantitative DON detection, providing a reference for trace detection of highly toxin mycotoxins.
研究领域:
食品科学
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分析化学
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纳米技术
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毒理学
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高影响因子
Results in Chemistry
·
2026
·
IF 4.2
Guanjie Wu,Yawen Du,Long Zhao,Quhuan Ma,Xiaofeng Shi,Xinyue Chen
This study developed a method based on mass spectrometry imaging (MSI) technology, initially addressing the chemical spatial heterogeneity of the needles of Cedrus deodara (Roxb.) G. Don (NCD). Meanwhile, based on the theory of meridians, the spatial metabolomics of NCD in cardiac tissue was studied. We first established phytochemical profiles of NCD and achieved in situ visualization of metabolite distribution. Comparative analysis across geographic origins and Pinaceae species revealed environmental modulation of secondary metabolism. Importantly, traditional meridian theory reported that NCD had the property of meridian tropism in the heart where it played a role in cardio protection. Spatial metabolomics analysis revealed that several mechanisms play an important role in cardioprotection, including the reactivation of TCA cycle energetics, the enhancement of glutathione-mediated antioxidant defenses, and the localized regulation of spermine metabolism. We conducted a reasoned regions of interest (ROI) analysis based on the physiological structure of the heart, and in combination with its own important physiological activities, providing a comprehensive study on spatial metabolomics. This “component identification-spatial imaging-meridian tropism analysis” framework systematically studied the characteristics of NCD from both in vivo and in vitro, providing a new idea for the research of natural products.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
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IF 2.983
Jiaxiang Yan,Qiang Shi,Kaihao You,Yanping Li,Qinghua He
Immunoassays play an important role in the monitoring and detection of mycotoxins in food. However, mycotoxin conjugates in the immunoassay system synthesized with toxic mycotoxin as raw material has seriously harmed the environment and human health during and after the performance. In this work, using mycotoxin deoxynivalenol (DON) as a model happen, a selected DON mimotope (phage-displayed peptide) that specifically binds to anti-DON antibody was served as a mimetic competing antigen in electrochemical immunosensor for ultrasensitive and green detection of DON. Under the optimized conditions, the mimotope peptide-based electrochemical immunosensor showed a dynamic range of 0.1–10,000 pg/ml and the limit detection (LOD) of 0.07 pg/ml. In addition, the accuracy and reproducibility of the established electrochemical immunosensor were evaluated by testing cross-reactivity and spiking DON negative samples. The result showed that mimotope peptide-based immunosensor has negligible cross-reactivity with other mycotoxins, and the average recovery of corn and wheat sample was 90.4%–118% and 101.3%–111%, respectively. Furthermore, the novel concept might provide potential applications to a general method for the immunosensor of various toxic small molecules.
研究领域:
分析化学
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食品安全
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环境科学
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生物技术
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PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2022
·Vol. 45
·期 9
·1600-1609
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IF 3.614
An Kang,Jialu Jiang,Qin Li,Xianjie Sheng,Yan Chen,Jiani Tan,Liu Li,Weixing Shen,DongXin Tang,Haibo Cheng,Dongdong Sun
Scutellaria barbata D. Don ( S. barbata ) is one of the most frequently used anticancer herb medicine in China. Mechanistic understanding of the biological activities of S. barbata is hindered by limited knowledge regarding its components and metabolic profile. In this study, ultra-high-performance liquid chromatography coupled with high resolution mass spectrometry (quadrupole time-of-flight mass spectrometry) was used to identify the chemical constituents in S. barbata and their metabolic profiles in rats. By applying cleavage rules and comparison with reference substances, 89 components were identified in S. barbata , which included 45 flavonoids, 28 diterpenoids, 10 phenolics, and 6 others. A total of 110 compounds, including 32 prototype compounds and 78 metabolites, were identified or tentatively characterized in vivo. Methylation, sulfonation, and glucuronidation were the main metabolic pathways, which could be attributed to the fact that several of the compounds in S. barbata have phenolic hydroxyl groups. This is the first systematic study on the chemical constituents and in vivo metabolic profile of S. barbata . The analytical method features a quick and comprehensive dissection of the chemical composition and metabolic profile of S. barbata and provides a basis for exploring its various biological activities.
研究领域:
中药学
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分析化学
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药理学
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高影响因子
Stress Biology
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2023
·Vol. 3
·期 1
·1-17
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IF 0
Duan Kaili,Shen Qifang,Wang Yu,Xiang Ping,Shi Yutong,Yang Chenfei,Jiang Cong,Wang Guanghui,Xu Jin-Rong,Zhang Xue
Plant hormones are important for regulating growth, development, and plant-pathogen interactions. Some of them are inhibitory to growth of fungal pathogens but the underlying mechanism is not clear. In this study, we found that hyphal growth of Fusarium graminearum was significantly reduced by high concentrations of IAA and its metabolically stable analogue 2,4-dichlorophenoxyacetic acid (2,4-D). Besides inhibitory effects on growth rate, treatments with 2,4-D also caused significant reduction in conidiation, conidium germination, and germ tube growth. Treatments with 2,4-D had no obvious effect on sexual reproduction but significantly reduced TRI gene expression, toxisome formation, and DON production. More importantly, treatments with 2,4-D were inhibitory to infection structure formation and pathogenesis at concentrations higher than 100 µM. The presence of 1000 µM 2,4-D almost completely inhibited plant infection and invasive growth. In F. graminearum , 2,4-D induced ROS accumulation and FgHog1 activation but reduced the phosphorylation level of Gpmk1 MAP kinase. Metabolomics analysis showed that the accumulation of a number of metabolites such as glycerol and arabitol was increased by 2,4-D treatment in the wild type but not in the Fghog1 mutant. Transformants expressing the dominant active FgPBS2 S451D T455D allele were less sensitive to 2,4-D and had elevated levels of intracellular glycerol and arabitol induced by 2,4-D in PH-1. Taken together, our results showed that treatments with 2,4-D interfere with two important MAP kinase pathways and are inhibitory to hyphal growth, DON biosynthesis, and plant infection in F. graminearum .
研究领域:
植物病理学
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分子生物学
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植物激素
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DOI
Journal of Integrative Medicine-JIM
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2024
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IF 4.2
Zhen Wang,Min Liu,Guang-xing Li,Liu Zhang,Kai-yue Ding,Si-qi Li,Bing-qing Gao,Peng Cheng,Hyok-Chol Choe,Lun-yue Xia,Yu-tong Yang,Yi Liu,Xue Sui,Jun-nan Ma,Lin Zhang
Objective Despite the combination of Scutellaria barbata D. Don and Scleromitrion diffusum (Willd.) R.J. Wang (SB-SD) being a recognized Chinese medicinal herbal pair that is commonly used in the treatment of ovarian cancer, there is a poor understanding of their pharmacological mechanisms. This study examines the antitumor properties and potential mechanisms of SB-SD on human ovarian cancer A2780 cells through a multi-omics approach, establishing a pharmacological basis for clinical utilization. Methods A range of mass ratios and reagents were used in the hot reflux extraction of SB-SD. The inhibitory effect of the SB-SD extracts on A2780 cell proliferation was assessed using the cell-counting kit 8 assay. A zebrafish tumor implantation model was used to evaluate the effects of SB-SD extracts on tumor growth and metastasis in vivo. Transcriptomics and proteomics were used to investigate alterations in biological pathways in A2780 cells after treatment with different concentrations of SB-SD extract. Cell cycle, cell apoptosis, intracellular free iron concentration, intracellular reactive oxygen species (ROS) concentration, malondialdehyde (MDA), and mitochondrial membrane potential were measured. Real-time quantitative reverse transcription polymerase chain reaction and Western blotting were utilized to investigate the effects of heme catabolism and ferritinophagy on ferroptosis induced by SB-SD extract in A2780 cells. Results The 70% ethanol extract of SB-SD (a mass ratio of 4:1) inhibited A2780 cell proliferation significantly with a half maximal inhibitory concentration of 660 μg/mL in a concentration- and time-dependent manner. Moreover, it effectively suppressed tumor growth and metastasis in a zebrafish tumor implantation model. SB-SD extract induced the accumulation of free iron, ROS, MDA, and mitochondrial damage in A2780 cells. The mechanisms might involve the upregulated expression of ferritinophagy-related genes microtubule-associated protein 1 light chain 3, autophagy-related gene 5, and nuclear receptor coactivator 4. Conclusions SB-SD extract effectively inhibited the development of ovarian cancer both in vitro and in vivo. Its mechanism of action involved inducing ferroptosis by facilitating heme catabolism and ferritinophagy. This herbal pair holds promise as a potential therapeutic option for ovarian cancer treatment and may be utilized in combination with routine treatment to improve the treatment outcomes of ovarian cancer patients.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2020
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IF 4.244
Yong Guo,Zhiyan Liu,Enhua Hou,Nannan Ma,Jiangping Fan,Cheng-Yun Jin,Ruige Yang
(+)-Nootkatone, a non-food bioactive natural bicyclic sesquiterpene ketone, is isolated from Chamaecyparis nootkatensis [D. Don] Spach as a renewable forest resource. In continuation of our effort to develop synthetic natural derived insecticides from non-food bioactive products, a small library of thirty N -(1,3- thiazol-2-yl)carboxamides fused (+)-nootkatone was prepared by molecular hybridization and characterized by 1 H/ 13 C NMR, HR-MS, and IR spectroscopy. Their insecticidal activities against Mythimna separata Walker and Plutella xylostella Linnaeus were evaluated. Compounds B6 , B7 , B9 , B19 – 21 and B24 showed better insecticidal activity against M. separata than the botanical insecticide azadirachtin , and their LC 50 values ranged from 0.55−0.68 mg/mL. Particularly, compound B9 exhibited 1.87-fold more pronounced insecticidal activity against M. separata than azadirachtin. The insecticidal activity of B21 against P. xylostella was 1.37-fold of that of azadirachtin. Through acetylcholinesterase (AChE) inhibitory activity and molecular docking studies, AChE may be the insecticidal target of B9 against M. separata . In addition, three pronounced compounds B9 , B21 and B24 exhibited low hemolytic and cytotoxic activities on normal mammalian cells. These findings will give insights into the further development of (+)-nootkatone derivatives as potentially synthetic natural derived insecticides for pest management.
研究领域:
植物化学
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昆虫学
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药物化学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 45
·35448-35452
·
IF 3.84
Xuejuan Chen,Xiaojing Bai,Huiyan Li,Bailin Zhang
An aptamer-based microcantilever array sensor for detection of fumonisin B-1 (FB1) was developed. The sensing cantilevers in the array were functionalized with self-assembled monolayers (SAMs) of thiolated FB1-specific aptamer, while the reference cantilevers were modified with 6-mercapto-1-hexanol SAMs to eliminate interference from the environment by detecting the deflection induced by nonspecific interactions. This differential deflection amplitude between sensing and reference cantilevers shows a linearity relation with the concentration of FB1 in the range from 0.1 to 40 μg mL−1, with a limit of detection of 33 ng mL−1 (S/N = 3). The sensor exhibits good specificity over ochratoxin A (OTA) and deoxynivalenol (DON). This biosensor provides a promising approach to detect FB1 in food and agricultural products.
研究领域:
生物传感器
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食品安全
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分析化学
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毒理学
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高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Shengyang Zhou,Xiaojun Zhu,Shanshan Song,Maozhong Sun,Hua Kuang,Chuanlai Xu,Lingling Guo
Mycotoxins, as secondary metabolites produced by fungi, have been the focus of researchers in various countries and are considered to be one of the major risk factors in agricultural products. There is an urgent need for a rapid, simple and high-performance method to detect residues of harmful mycotoxins in agricultural foods. We have developed a gold nanoparticle-based multiplexed immunochromatographic strip biosensor that can simultaneously detect fifteen mycotoxins in cereal samples. With this optimized procedure, five representative mycotoxins, deoxynivalenol (DON), zearalenone (ZEN), T-2 toxin (T-2), tenuazonic acid (TEA) and alternariol (AOH) were detected in the range of 0.91–4.77, 0.04–0.56, 0.11–0.68, 0.12–1.02 and 0.09–0.75 ng/mL, respectively. The accuracy and stability of these measurements were demonstrated by analysis of spiked samples with recoveries of 91.8%–115.3% and coefficients of variation <8.7%. In addition, commercially available samples of real cereals were tested using the strips and showed good agreement with the results verified by LC-MS/MS. Therefore, Our assembled ICA strips can be used for the simultaneous detection of 5 mycotoxins and their analogs (15 mycotoxins in total) in grain samples, and the results were consistent between different types of cereal foods, this multiplexed immunochromatographic strip biosensor can be used as an effective tool for the primary screening of mycotoxin residues in agricultural products.
研究领域:
农业科学
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食品安全
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分析化学
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纳米技术
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毒理学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）