CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Tong Xia,Zhilin Xi,Lianquan Suo,Chen Wang
A highly efficient coal dust suppressant with low initial viscosity and high adhesion-solidification properties was fabricated by reinforcing a polyacrylic acid hydrosol with dopa and glutathione (DG) redox system. The synthesis mechanism and dust suppression properties of the dust suppressant were investigated by ultraviolet–visible spectroscopy, high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy experiments combined with density functional theory calculations. The polyacrylic acid hydrosol was synthesized by free radical polymerization with an optimal concentration ratio of 0.36 % ammonium persulfate to 9 % acrylic acid; the DG redox system presented a catechol-quinone dynamic equilibrium, and the dopa polymer reinforcing adhesion was synthesized by the reverse dismutation and polymerization of dopa and dopaquinone. Results revealed that the dust suppressant system formed a multiple hydrogen bonding cross-linking network, dominated by strong double hydrogen bonding interaction formed between the carboxyl groups of dopa polymer and polyacrylic acid, and synergized by hydrogen bonds between the carboxyl groups of glutathione and PAA, and between the hydroxyl and carboxyl groups of dopa and dopaquinone. Moreover, the − OH and − COOH groups in the dust suppressant system were more tightly bonded after dehydration and can form a mesh film to effectively adhere and solidify coal dust, and the coal crust film did not raise dust at 20.526 ± 0.083 m/s wind speed.
研究领域:
环境科学
·
材料化学
·
矿山安全工程
·
高分子科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shiming Tang,Zhilin Ouyang,Ying Huo,Daocheng Liao,Zhiteng Zhang,Ying Lin,Suiping Zheng
Hydroxytyrosol has been proven beneficial to human health. However, the process involving the conversion of L-DOPA to 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) in hydroxytyrosol biosynthesis typically required the simultaneous use of decarboxylase and oxidative deaminase. In addition, phenylacetaldehyde reductase from Solanum lycopersicum (SlPAR) in hydroxytyrosol biosynthesis exhibits poor thermal stability. In this study, we unexpectedly discovered that L-DOPA decarboxylase from Pseudomonas putida (PpDODC) exhibits weak dual-functional activity for both decarboxylation and oxidative deamination of L-DOPA. Through a dual-function reshaping strategy, the best dual-functional mutant PpDODC/Y79F/Y324F achieved an enzyme activity of 0.95 U/mg and exhibited a 256.8-fold increase in activity compared to the wild-type. Through rational design of SlPAR, the optimal mutant SlPAR/G52D/V188I/N234W/Q286P (SlPAR-M4) maintained stability after 12 h' treatment at 40 °C. Based on these mutants, we established a simplified cascade to synthesize hydroxytyrosol from L-DOPA, achieving a hydroxytyrosol yield of 31.4 mM from 32 mM L-DOPA in a 5-hour reaction. This process achieved the highest molar conversion rate (98.2 %) currently reported for the synthesis of hydroxytyrosol from L-DOPA. This study provides a novel solution for hydroxytyrosol synthesis from a new perspective, and the mutants hold promise for widespread application in biotransformation studies of hydroxytyrosol and other structurally-similar compounds.
研究领域:
生物化学
·
生物技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 18
·6088
·
IF 4.927
Ke Shun Hu,Chong Le Chen,Huan Ru Ding,Tian Yu Wang,Qin Zhu,Yi Chen Zhou,Jia Min Chen,Jia Qi Mei,Sheng Hu,Jun Huang,Wei Rui Zhao,Le He Mei
Salvianic acid A (SAA), as the main bioactive component of the traditional Chinese herbSalvia miltiorrhiza, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA froml-DOPA was developed. In the first step,l-DOPA was transformed to 3,4-dihydroxyphenylalanine (DHPPA) using engineeredEscherichia colicells expressing membrane-bound L-amino acid deaminase fromProteus vulgaris.After that, the unpurified DHPPA was directly converted into SAA by permeabilized recombinantE. colicells co-expressingd-lactate dehydrogenase fromPediococcus acidilacticiand formate dehydrogenase fromMycobacterium vaccaeN10. Under optimized conditions, 48.3 mM of SAA could be prepared from 50 mM ofl-DOPA, with a yield of 96.6%. Therefore, the bioprocess developed here was not only environmentally friendly, but also exhibited excellent production efficiency and, thus, is promising for industrial SAA production.Keywords:salvianic acid A;l-DOPA;membrane-boundl-amino acid deaminases;biocatalysis;molecular biology;biological engineering
研究领域:
生物技术
·
药学
·
生物化工
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产品关联
DOI
PubMed
高影响因子
SYNTHETIC METALS
·
2018
·
IF 2.526
Junlong Niu,Liangmei Rao,Ping Liu,Wenwen Zhang,Wenna Zhang,Yinxiu Zuo,Keke Chai,Shuai Chen,Jingkun Xu,Xuemin Duan
Two novel chiral 3,4-dihydro-2 H -thieno[3,4- b ][1,4]dioxepine (ProDOT)-based electrochemical sensor , employing a pair of glassy carbon electrodes modified with chiral poly(2-Phenyl-propionic acid 3,4-dihydro-2 H -thieno[3,4- b ][1,4]dioxepin-3-ylmethyl ester) (PProDTM-PP), were utilized for the enantioselective detection of chiral 3,4-dihydroxyphenylalanine (DOPA). The enantioselective recognition between chiral PProDTM-PP modified glassy carbon electrodes and chiral DOPA was studied by using different electrochemical technologies. The redox activity and electrochemical stability of chiral PProDTM-PP was good. ( R )-PProDTM-PP and ( S )-PProDTM-PP showed higher peak current responses toward D -DOPA and L -DOPA, respectively. Furthermore, the recognition efficiencies of chiral PProDTM-PP are between 1.48 and 1.89, which is higher than chiral PEDOT-based electrochemical sensors (around 1.10). The chiral PProDTM-PP provides an alternative to chiral recognition.
研究领域:
生物化学
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Zhang Junzhi,Lin Binyan,Zhang Ying,Hu Xiaochao,Liu Tongtong,Liu E-Hu,Liu Shijia
Background: Baitouweng decoction (BTW) is a classic botanical drugs formula that has been widely used clinically for the treatment of gut-related disorders in China. However, its role in ameliorating ulcerative colitis (UC) remains to be explored.Purpose: The study aimed to determine the therapeutic efficacy and potential mechanism of action of BTW on dextran sodium sulfate (DSS)-induced colitis mice.Methods: In vivo: 3.5% DSS-induced experimental colitis mice were treated with BTW (Pulsatilla chinensis (Bunge) Regel, Phellodendron chinense C. K. Schneid, Coptis chinensis Franch and Fraxinus chinensis Roxb), kynurenine or DOPA decarboxylase (DDC) inhibitor (carbidopa). In vitro: Caco-2 cells were stimulated with TNF-α to activate inflammation and later treated with various concentrations of BTW and carbidopa. Model evaluation included body weight, disease activity index (DAI) score, colon length and histopathology. Cytokine levels were measured by flow cytometry. Protein levels were analyzed by proteomics and functionally annotated. The levels of tryptophan metabolites in mouse serum and colon were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Alcian Blue/Phosphate Acid Schiff (AB/PAS) staining, immunohistochemistry and western blot were used to assess the intestinal barrier function and detect the protein expression levels.Results: BTW significantly reduced the DAI, ameliorated colonic injury and regulated inflammatory cytokines in DSS-induced colitis mice. The botanical drugs formula also promoted intestinal epithelial barrier repair by enhancing the expression of the tight junction (TJ) proteins. Tryptophan metabolic signaling pathway was significantly enriched in DSS-induced UC mice, and BTW decreased the level of kynurenine, increased indole metabolites. The therapeutic effect of BTW was evidently reduced when kynurenine was given to mice. Also, BTW promoted DDC protein expression and activated the aryl hydrocarbon receptor (AHR)/IL-22 signaling pathway.Conclusion: BTW improves ulcerative colitis by promoting DDC expression, regulating the conversion of tryptophan metabolism from the kynurenine pathway to the indole metabolism pathway, thereby modulating tryptophan metabolism to increase indole metabolites, and activating AHR receptors to restore intestinal barrier function.
研究领域:
药理学
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胃肠病学
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免疫学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2011
·Vol. 24
·期 2
·332-337
·
IF 2.721
Qian Han,Qiao Chen,Yonghua Wang,Juan Zhou,Yingzi Fu
A simple sensor was obtained through N -isobutyryl- L -( D )-cysteine enantiomers self-assembled monolayer. It was demonstrated that the N -isobutyryl-cysteine modified gold electrodes can enantioselectively recognize 3,4-dihydroxyphenylalanine (dopa) enantiomers. The electrocatalytic behaviors of enantiomers were analyzed with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Selectivity of the chiral surface was estimated by investigating two mixture enantiomers (dopa-ascorbic acid and dopa-tyrosine), it certified that the sensor was only satisfactorily used to specific recognize dopa enantiomers. The possible mechanism for the enantioselective recognition was discussed.
研究领域:
电分析化学
·
手性识别
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传感器与生物传感器
·
对映选择性检测
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DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Kun Yang,Ruyi Wang,Junjian Lu,Jin Wang,Xuewei Liao,Chen Wang
Recognition and separation of chiral isomers are of great importance in both industrial and biological applications. However, owing to identical molecular formulas and chemical properties of enantiomers , signal transduction and amplification are still two major challenges in chiral sensing. In this study, we developed an enantioselective device by integrating chiral covalent organic framework nanosheets (CONs) with nanochannels for sensitive identification and quantification of enantiomers . Using 3,4-dihydroxyphenylalanine (DOPA) as the model analyte, the as-prepared chiral nanofluidic device exhibits a remarkable chiral recognition ability to l -DOPA than d -DOPA. More importantly, due to the chelation of DOPA with Fe 3+ ions, it can efficiently block the ion transport through channel and shield the channel surface charge, which will amplify the difference in the electrochemical response of l -DOPA and d -DOPA. Therefore, a sensitive chiral recognition can be achieved using the present nanofluidic device coupled using electrochemical amplification strategy. Notably, using this method, an ultra-low concentration of l -DOPA (as low as 0.21 pM) can be facilely and successfully detected with a linear range of 1 pM–10 μM. This study provides a reliable and sensitive approach for achieving highly selective detection of chiral molecules.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
·
2013
·
IF 0.9
Yihang Lv,Hui Ma,Dongdong Gao,Yi Zhong,Hong Xu,Zhiping Mao
Adhesive polymers, which contain an unusual amino acid, 3,4-dihydroxyphenylalanine (DOPA), can bind strongly to all organic or inorganic surfaces. However, due to DOPA’s poor solubility in organic solvents, it is difficult for us to get desired adhesive polymers. In this paper, poly(N-benzyloxycarbonyl-3,4-dihydroxyphenylalanine) (PNBD) was synthesized based on the modified L-DOPA. Gel Permeation Chromatography was used to analyze the molecular weight of PNBD. The structure of PNBD was characterized by Fourier Transform Infrared Spectroscopy and Hydrogen-1 Nuclear Magnetic Resonance. The adhesive properties of PNBD were determined by lap shear tensile strength tests. The results indicated that PNBD was prepared successfully and had formidable adhesive performance on the various substrates.
研究领域:
材料科学
·
生物化学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Jian Yang,Jiale Jin,Haojie Yu,Yun Wang,Li Wang,Xiaowei Liu,Dongyu Wang,Yudi Huang,Chenguang Ouyang,Yu Wang,Yichuan Hong,Shuning Ren,Jian Hu,Jingyi Feng
Severe trauma not only damages the skin, but also affects the bones, causing osteoarthritis or bone defects. Traditional treatment strategies (such as sutures, metal stents, etc. ) have problems with the need for a second surgery and difficulty in degradation, which cannot meet the treatment expectations of clinical doctors well. As the novel biomedical material, hydrogels have great potential in the treatment of bone defects due to the advantages of seamless filling, degradability and easy operation. Here, we designed a composite hydrogel (Gel-Gym/HS-Dopa) composed of glycidyl methacrylate-modified gelatin (Gel-Gym) and dopamine-functionalized chondroitin sulfate (HS-Dopa). The hydrogel exhibited good tissue adhesion, self-healing, biodegradability, anti-inflammatory and biocompatibility. In animal experiments, Gel-Gym/HS-Dopa hydrogel could significantly promote the rat skin wound healing and bone defect reconstruction in vivo . Therefore, the hydrogel was promising as a potential deep trauma dressing.
研究领域:
生物医学材料
·
组织工程
·
创伤修复
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2018
·
IF 2.275
Xiao-Ling Tang,Hui Suo,Ren-Chao Zheng,Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) naturally catalyzes the reversible β-elimination of l -tyrosine to phenol, pyruvate and ammonium. With its reverse reaction (synthetic activity), l -tyrosine and its derivatives could be synthesized with high atom economy, which are widely used in pharmaceutical industries . In this study, a high-throughput screening method for synthetic activity of TPL was developed. One of the substrate, sodium pyruvate was found to react with salicylaldehyde under alkali condition, forming a yellow color compound. The concentration of sodium pyruvate can be quantified according to the absorbance of the colorimetric compound at wavelength of 465 nm and the activity of TPL could be screened according to the decrease of the absorbance. After optimization of the colorimetric reaction conditions, the established high-throughput screening method was successfully used for screening of TPL with enhanced activity for l -DOPA synthesis. The confirmed sensitivity and accuracy demonstrated the feasibility and application potential of this screening method.
研究领域:
生物化学
·
制药工程
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产品关联
DOI
PubMed
高影响因子
ChemPlusChem
·
2018
·Vol. 83
·期 11
·1038-1043
·
IF 3.205
Song Wang,Hejin Jiang,Li Zhang,Jian Jiang,Minghua Liu
Graphical Supramolecular gels formed from hemin and histidine amphiphiles were tested as artificial enzymes to catalyse the oxidation of the chiral substrate L-DOPA to L-dopachrome. The supramolecular gel made up of left-handed helices enhanced the catalytic activity of hemin effectively, and more interestingly, it exhibited enantioselectivity. Supramolecular co-assembly provides an efficient way to combine structural and functional units through noncovalent interactions and produce materials with enhanced performance. Here, using a chiral amphiphilic histidine derivative (LHC18 or DHC18) as a gelator, we have successfully co-assembled the gelator and hemin through gelation. During the co-assembly, the chiral information of the gelator was transcribed into the assemblies, and thus right- and left-handed helical ribbons from LHC18-Hemin and DHC18-Hemin co-assemblies were obtained, respectively, which were confirmed by CD spectra and SEM observations. These helical ribbons were used as artificial enzymes to catalyse the oxidation reaction of 3,4-dihydroxy-L-phenylalanine (L-DOPA). The catalytic activity of hemin in supramolecular gels can be effectively enhanced over that of free hemin. Moreover, by virtue of induced chirality, the supramolecular gels of LHC18-Hemin and DHC18-Hemin exhibited obvious enantioselective activity. This work provides a way to combine chirality transfer and supramolecular asymmetric catalysis.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Lei Ding,Chundan Ye,Junchen Zhu,Cuirong Sun
Chiral impurity analysis presents significant challenges due to limitations of detection methods. Levodopa (L-Dopa) is the primary medication for Parkinson's disease treatment for over 50 years. However, the detection of its chiral impurity is difficult due to the similarity of polarity. This study presents a novel analytical method for the quantitative detection of trace amount of D-Dopa, as well as other three impurities. The method demonstrated excellent chromatographic resolution for D-Dopa and L-Dopa (resolution = 4.15) and achieved a derivatization efficiency of 97% using a chlorine-labeled chiral probe (D-BPCl) for chemical derivatization, followed by analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a conventional C18 column. Meanwhile, D-BPCl showed strong chiral selectivity on D-Dopa, and the ratio of mass spectrometric response for labeled D-Dopa to that of L-enantiomers was 2.78-fold under the same condition. The developed method demonstrates high sensitivity with detection limits of 7.88 μ g L -1 for D-Dopa, and 9.85 μ g L -1 for L-Dopa, with good linearity for every analyte (R 2 > 0.99), recoveries ranging from 94.1% to 106.8%, and high repeatability with relative standard deviation (RSD) values predominantly below 5%. Then the method was applied to analyze fifteen batches of levodopa tablets from five manufacturers. D-Dopa was successfully detected with the corresponding content ranging from 0.15% to 0.44%, which suggests that D-Dopa are more likely generated during the production process. The study underscores the significant safety concerns regarding Dopa-containing medications.
研究领域:
药物分析
·
药物质量控制
·
手性分离
·
药物代谢
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）