MOLECULES
·
2020
·Vol. 25
·期 8
·1825
·
IF 3.267
Yuanyang Dong,Qihang Hou,Meng Sun,Jingjing Sun,Bingkun Zhang
Plantago asiaticaL. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function ofPlantago asiaticaL. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in cells. Hydrogen-peroxide-induced oxidative stress led to decreased cell viability, impaired intercellular junction, and damage to the cell membrane and DNA. Furthermore, aesculetin ameliorated decreased cell viability induced by hydrogen peroxide via upregulation of antioxidant related genes, and apigenin also protected against H2O2mainly by improving the glutathione (GSH) antioxidant system, such as increasing the activity of glutathione peroxidase (GPX), glutathione reductase (GR), and the ration of GSH/glutathione disulfide (GSSG). Above all, these findings suggest that aesculetin and apigenin may be bioactive compounds for antioxidant function inPlantago asiaticaL.Keywords:antioxidants;compound isolation;DPPH;Plantago asiaticaL.;aesculetin;apigenin
研究领域:
植物学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
·
2024
·Vol. 50
·期 4
·1466-1475
·
IF 1.1
Lu Liushen,Qin Ting,Chen Kai,Xie Jun,Pan Liangkun,Xi Bingwen
Abstract Objective: Resveratrol, a natural product found in plants, is a polyphenolic compound with strong antioxidant activity. In order to enhance the antioxidant activity of resveratrol, we have used a natural product emodin, which has multiple hydroxyl group structure similar to resveratrol. Methods: In this study, we investigated the antioxidant activities of resveratrol and emodin in vitro respectively, including their abilities to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, hydroxyl radicals, and superoxide radicals. Results and Discussion: our results demonstrated that the combination of resveratrol and emodin significantly enhances antioxidant activity. And the process of their DPPH radical scavenging follows a first-order reaction kinetics equation. Finally, a possible mechanism for the scavenging of DPPH free radicals by resveratrol and emodin was proposed. Conclusions: We found that emodin combined with resveratrol can increase antioxidant activity. This study provides a new sight to enhance antioxidant activity.
研究领域:
药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2446
·
IF 4.6
Dan Wu,Qile Xia,Huilin Huang,Jinhu Tian,Xingqian Ye,Yanbin Wang
When the total phenolic content (TPC) and antioxidant activity of sea buckthorn juice were assayed by spectrophotometry, the reaction solutions were not clarified, so centrifugation or membrane treatment was needed before determination. In order to find a suitable method for determining TPC and antioxidant activity, the effects of centrifugation and nylon membrane treatment on the determination of TPC and antioxidant activity in sea buckthorn juice were studied. TPC was determined by the Folin-Ciocalteau method, and antioxidant activity was determined by DPPH, ABTS, and FRAP assays. For Treatment Method (C): the sample was centrifuged for 10 min at 10,000 rpm and the supernatant was taken for analysis. Method (CF): The sample was centrifuged for 10 min at 4000 rpm, filtered by Nylon 66 filtration membranes with pore size of 0.22 μm, and taken for analysis. Method (F): the sample was filtered by Nylon 66 filtration membranes with pore size of 0.22 μm and taken for analysis. Method (N): after the sample of ultrasonic extract solution reacted completely with the assay system, the reaction solution was filtered by Nylon 66 filtration membranes with pore size of 0.22 μm and colorimetric determination was performed. The results showed that centrifugation or transmembrane treatment could affect the determination of TPC and antioxidant activity of sea buckthorn juice. There was no significant difference (p> 0.05) between methods (CF) and (F), while there was a significant difference (p< 0.05) between methods (C) (F) (N) or (C) (CF) (N). The TPC and antioxidant activity of sea buckthorn juice determined by the four treatment methods showed the same trend with fermentation time, and the TPC and antioxidant activity showed a significant positive correlation (p< 0.05). The highest TPC or antioxidant activity measured by method (N) indicates that method (N) has the least loss of TPC or antioxidant activity, and it is recommended for sample assays.Keywords:DPPH;ABTS;FRAP;centrifugation;transmembrane
研究领域:
食品科学
·
分析化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic and Nano-Metal Chemistry
·
2022
·
IF 1.514
Rong Chen,Fenghe Chen,Mengyang Sun,Renyu Zhang,Songshuai Wu,Chuangang Meng
In this work, we report a controllable green synthesis of gold nanoparticles (AuNPs) using chlorogenic acid (CGA) as the only reducing and stabilizing agent. The well-dispersed AuNPs are obtained due to the protection of CGA against aggregation. The resulting AuNPs are controllable in size and shape mainly by changing the molar ratio of HAuCl4 to CGA in reductive process. With an increase in CGA concentration, the average size of AuNPs increased, which resulted in different morphologies from spherical to flower-like shapes. The dropping speed of gold precursor is also found to influence the size of AuNPs in different trends. Additionally, the advantages of CGA for synergistic biological activities are demonstrated through the enhanced antioxidant activity of AuNPs with CGA for scavenging of DPPH radicals. The controllable size and shape of nanoparticles are potentially important in nanomedical application.
研究领域:
纳米医学
·
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 42
·e202203493
·
IF 2.307
Xiang Yu,Mengyu Liu,Zaiheng Huang,Yanzhi Tao,Hao Yang,Hongbin Gan,Guangzai Nong
Graphical Quercetin and selenite were combined to form a quercetin selenium complex at 100°C. The mass of the product was 30.3 % of the mass of quercetin and the structure was analyzed in depth. The complex had a DPPH antioxidant activity of 97.91 %, and the prepared skin cream containing 1 % of the complex had an antioxidant activity of 82.4 %. Flavonoid complexes have more biological activities than parent flavonoids, including antioxidant, anticancer, antibacterial, and many others. Selenium is an essential trace element for human body. Therefore, a quercetin-Se complex was synthesized and analyzed in this paper, getting the results as following: (1) A light- yellow product was obtained with the max yield of 30.30 %. (2) The complex molecule contains two quercetin units and a selenoxyl group, called selenious acid acyl diquercetin. (3) It has a strong antioxidant activity with a maximum DPPH scavenging rate of 97.71 %. In addition, we prepared a skin cream containing 1 % quercetin selenium complex, and it has excellent antioxidant activity with a DPPH scavenging rate of 82.4 %. Therefore, the generated selenious acid acyl diquercetin is a potential antioxidant material for skin care products.
研究领域:
化学
·
生物学
·
药学
✓ 已验证
DOI
高影响因子
Frontiers in Nutrition
·
2020
·
IF 3.365
Luo Xuguang,Wang Jin,Chen Haiqiang,Zhou Aimei,Song Mingyue,Zhong Qingping,Chen Hanmin,Cao Yong
Finger citron (Citrus medica L. var. sarcodactylis Swingle) is a traditional Chinese herb and considered as a healthy food. Flavonoids are the major bioactive substances in Finger citron. In this study, the major flavonoids of finger citron (FFC) were purified with AB-8 macroporous resins, and then three of them were identified as diosmetin-6-8-di-C-glucoside, hesperidin and diosmetin-6-C-glucoside, and other two were preliminarily inferred as limocitrol 3-alpha-l-arabinopyranosyl-(1->3)-galactoside and scutellarein 4′-methyl ether 7-glucoside by high-performance liquid chromatography and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry. Further, their antioxidation and antiaging activities were determined in vitro and in vivo. In vitro, chemical assays revealed that the purified FFC had strong antioxidative activity as demonstrated by its strong DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid) diammonium salt] radical scavenging activities and ORAC (oxygen radical absorbance capacity). In vivo, the purified FFC significantly increased the mean and maximum lifespan of Caenorhabditis elegans by 31.26 and 26.59%, respectively, and showed no side effects on their physiological functions. Under normal and oxidative stress conditions, purified FFC reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde, while increased superoxide dismutase (SOD) and catalase (CAT) enzyme activities in C. elegans. Together, we successfully identified three major substances in purified FFC of finger citron and determined the excellent antiaging activity of FFC, which is attributed to its strong antioxidative activity and effect on homeostasis of ROS.
研究领域:
植物化学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Dongmei Li,Xiaokang Na,Haitao Wang,Yisha Xie,Shuang Cong,Yukun Song,Xianbing Xu,Bei-Wei Zhu,Mingqian Tan
Food-borne nanoparticles have received great attention because of their unique physicochemical properties and potential health risk. In this study, carbon dots (CDs) formed during one of the most important chemical reactions in the food processing field, the Maillard reaction from the model system including glucose and lysine, were investigated. The CDs purified from Maillard reaction products emitted a strong blue fluorescence under ultraviolet light with a fluorescent quantum yield of 16.30%. In addition, they were roughly spherical, with sizes of around 4.3 nm, and mainly composed of carbon, oxygen, hydrogen, and nitrogen. Their surface groups such as hydroxyl, amino, and carboxyl groups were found to possibly enable CDs to scavenge DPPH and hydroxyl radicals. Furthermore, the cytotoxicity assessment of CDs showed that they could readily enter HepG2 cells while causing negligible cell death at low concentration. However, high CDs concentrations were highly cytotoxic and led to cell death via interference of the glycolytic pathway.
研究领域:
食品科学
·
生物医学
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Ji Xiaolong,Guo Jianhang,Pan Feibing,Kuang Fengjun,Chen Haiming,Guo Xudan,Liu Yanqi
A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food.
研究领域:
植物化学
·
食品科学
·
天然产物化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 10
·2663
·
IF 3.098
Tian Chai,Xiao-Bo Zhao,Wei-Feng Wang,Yin Qiang,Xiao-Yun Zhang,Jun-Li Yang
Gx-50 is a bioactive compound for the treatment of Alzheimer’s disease (AD) found in Sichuan pepper (Zanthoxylum bungeanum). In order to find a stronger anti-AD lead compound, 20 gx-50 (1–20) analogs have been designed and synthesized, and their molecular structures were determined based on nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis, as well as comparison with literature data. Compounds1–20were evaluated for their anti-AD potential by using DPPH radical scavenging assay for considering their anti-oxidant activity, thioflavin T (ThT) fluorescence assay for considering the inhibitory or disaggregate potency of Aβ, and transgenicDrosophilamodel assay for evaluating their rescue effect on memory loss. Finally, compound13was determined as a promising anti-AD candidate.Keywords:Alzheimer’s disease;gx-50;DPPH assay;ThT assay;transgenicDrosophilamodel assay
研究领域:
药理学
·
神经科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
·
2014
·Vol. 33
·期 1
·159-168
·
IF 0.684
Wang Xueqin,Xing Ronge,Liu Song,Yu Huahua,Li Kecheng,Chen Zuoyuan,Li Pengcheng
Mackerel ( Pneumatophorus japonic u s ) proteins were hydrolyzed by five proteases: trypsin, papain, neutrase, acid protease, and flavourzyme. The hydrolysate treated by neutrase exhibited the highest antioxidant activity. Response surface methodology (RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate (MPH) with the highest DPPH radical scavenging activity. The MPH was fractioned using a series of ultrafiltration membranes and five fractions, namely, MPH-I (>10 kDa), MPH-II (10–2.5 kDa), MPH-III (1–2.5 kDa), MPH-IV (0.4–1 kDa), and MPH-V (below 0.4 kDa), were obtained. DPPH radical scavenging activity, reducing power, hydroxyl radical scavenging activity, and the lipid peroxidation inhibition capability of these fractions were evaluated. The fractions in molecular weights <2.5 kDa (MPH-III, MPH-IV, and MPH-V), which occupied 93.4% of the total fractions, showed the strongest antioxidant activity; and the antioxidant activities of the three fractions are similar to each other. Using SP Sephadex C-25 and Sephadex G-25 columns, eight fractions were obtained from the MPH (<2.5 kDa). The isolated peptide I (1 664 kDa) displayed the highest DPPH radical scavenging activity. Therefore, MPH is a potential source of antioxidant peptides.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 12
·e16074
·
IF 2.19
Xia Li,Guozhu Zhang,Jing Li,Tiemin Jiang,Huiying Chen,Peijun Li,Yuan Guan
In this paper, water extracted polysaccharides from Passiflora edulis peel (WPEP) was degraded by ascorbic acid-hydrogen peroxide (Vc-H 2 O 2 ). The degradation conditions were optimized by response surface methodology (RSM). The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate of degraded WPEP (DWPEP) was 45.38% under optimal conditions (Vc-H 2 O 2 concentration 9.3 mM, temperature 52°C, time 1.4 hr). And the molecular weight (Mw) of DWPEP (38 kDa) was 32.20% (WPEP was 118 kDa). WPEP and DWPEP were mainly composed of galacturonic acid. Their backbone structures, functional groups and β-glycoside linkage patterns are similar, however, still with some differences. Antioxidant tests showed that WPEP and DWPEP had strong DPPH radical scavenging ability (93.03% and 81.70%), moderate hydroxyl radical scavenging ability (53.99% and 26.59%), and low reducing power (0.698 and 0.52). In conclusion, these differences in structures and properties may be an important factor affecting the antioxidant activity of polysaccharides. Novelty impact statement In this paper, the Vc-H 2 O 2 system was used to degrade the polysaccharides extracted from Passiflora edulis peel. The molecular weight, intrinsic viscosity, and rigid conformation changed obviously. The degraded polysaccharides are valuable to expand their application and to improve the practical efficacy.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Applied Spectroscopy
·
2023
·Vol. 90
·期 4
·883-892
·
IF 0.7
Shi Ying,Guo Ya-Dan,Mi Yi-Duo,Cheng Jia,Dong Wen,Zhang Gao-Feng,Zeng Yi
The antioxidant activities of flavonoid mixtures can be used to investigate the synergistic antioxidant mechanism of flavonoids. The antioxidant capacities of three flavonoids (quercetin, baicalein, and daidzein) and β-carotene in binary and ternary mixtures with kaempferol were analyzed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay by means of absorption spectroscopy. The results showed that the number of hydroxyl groups and o-hydroxyl groups had a significant effect on the antioxidant activity of the flavonoids, and the mixture of kaempferol, quercetin, and baicalein showed optimal synergistic antioxidant activity. Compared with quercetin and baicalein, kaempferol had the fastest inhibition rate, and multiple prolonged kinetic processes associated with the scavenging of DPPH radicals occurred in mixtures of kaempferol with the other flavonoids and β-carotene. Kaempferol has a potential synergistic antioxidant effect when mixed with daidzein and β-carotene, and the results suggested that this may be due to the regeneration of kaempferol after antioxidation. By means of classic UV-Vis spectroscopy, reaction details of the synergistic antioxidant process of DPPH radical scavenging by flavonoids can be obtained.
研究领域:
食品科学
·
植物化学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）