CEREAL CHEMISTRY
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2019
·Vol. 96
·期 6
·981-993
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IF 1.289
Zhongwei Ji,Ruixue Feng,Jian Mao
Background and objectives Prolamins are the primary component of millet protein, constituting over 50% ( w / w ) of the total protein. This study aimed to prepare and identify bioactive peptides from millet prolamins (MP). Findings Ultrasonic and heat treatment could change the secondary structure conformation of the protein and invert part of the α-helices into β-sheets or β-turns. After these treatments, the degree of hydrolysis increased. The alcalase-hydrolyzed fraction with molecular weight <1 kDa exhibited the highest antioxidant activity. The bioactive peptides were separated and purified by gel-filtration chromatography and reversed-phase high-performance liquid chromatography. Two novel peptides with molecular weights of 522.3 and 785.5 Da were identified as Pro-Phe-Leu-Phe (PFLF) and Ile-Ala-Leu-Leu-Ile-Pro-Phe (IALLIPF), respectively. Both peptides showed high antioxidant activity, especially PFLF, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability (RSA) of which was 149 μM TE/g protein, and the Oxygen radical absorbance capacity (ORAC) value reached 1,180 μM TE/g protein. Conclusions The results demonstrate that ultrasonic and heat treatment could enhance hydrolysis of MP by protease and antioxidant peptides with high DPPH RSA as well as ORAC value were purified and obtained. Significance and novelty Ultrasonic and heat treatment increased degree of MP hydrolysis. Two antioxidant peptides were successfully obtained from millet prolamins.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Li Zhang,Xinquan Liu,Yongjie Wang,Guangpu Liu,Zhen Zhang,Zhongxi Zhao,Huiling Cheng
Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time. Polysaccharides have been found to be important bioactive compounds in the jujube. This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv. Muzao . Water-soluble polysaccharides were extracted using an ultrasonically assisted extraction method. The purified polysaccharides (HJP) were obtained by deproteinization and decoloration. Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography. The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions. The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions. Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). In conclusion, the polysaccharides from Zizyphus jujuba cv. Muzao were discovered to have antioxidative and immunological activities.
研究领域:
药理学
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生物化学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2023
·
IF 8.8
Liqi Ai,Lei Liu,Lin Zheng,Yang Liu,Baoguo Sun,Guowan Su,Jucai Xu,Yajun Chen,Mouming Zhao
Food-derived antioxidant peptides have become the focus of research due to their high safety and low cost. However, the discovery is suffering from a low efficient and empirical approach, involving multi-step off-line separation and identification. In this work, an on-line stop-flow RPLC × SEC -MS/DPPH radical scavenging activity analysis system was developed. For optimization, the conditions: 10 m reaction loop, 200 μM DPPH radical concentration, 40℃ temperature and 0.06 % formic acid were recommended. The system was fully validated by its application in glutathione analysis. The system was further applied in analysis of complex mixed standards, and the dipeptides GC (Gly-Cys) and CW (Cys-Trp) with relatively strong DPPH radical scavenging activity were validated. Maize protein hydrolysates were used for tests and the peptide AC (Ala-Cys) of high probability with strong DPPH radical scavenging activity was identified, demonstrating a high potential of the system. This would help to facilitate the discovery of antioxidative peptides in the future.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
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IF 4.052
Shuang Zang,Sizhu Tian,Jia Jiang,Dandan Han,Xinyu Yu,Kun Wang,Dan Li,Dayong Lu,Aimin Yu,Ziwei Zhang
Twenty-one kinds of fruits including strawberry, mulberry, lemon, banana, etc. were measured for antioxidant capacity based on their ability to scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Vitamin C equivalent antioxidant capacity (VCEAC) was used to quantify antioxidant capacity of the studied fruits. The results were expressed as mg of ascorbic acid equivalent per 100 g fruit. Each fruit was divided into two parts: harvest part (fresh fruit analyzed immediately), and liquid nitrogen frozen part (fruit frozen and pulverized in liquid nitrogen). Antioxidant capacities of both fresh and frozen fruits were determined, and VCEAC values were proved to have no significant difference. For the frozen fruits, the antioxidant capacities were measured by electron spin resonance spectroscopy (ESR) and UV–vis spectrometry. VCEAC values obtained with UV–vis and ESR range from 11.48 to 345.75 mg/100 g and 7.01 to 366.26 mg/100 g. Experimental results indicated that VCEAC values obtained by two methods were highly correlated.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT ORGANIC SYNTHESIS
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2025
·Vol. 22
·期 6
·754-768
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IF 2.5
Dat Van Nguyen,Ly Duou Tran,Phuong Uyen Ngoc Vu,Luc Van Meervelt,Mai Ngoc Thi Nguyen,Anh Lan Ngo,Hoan Quoc Duong
Backgroundo-Aminophenol derivatives are of particular interest for their diverse biological activities and potential therapeutic applications. Such as, antioxidant, antibacterial, and cytotoxic activities.ObjectivesThis study aimed to design and synthesize a series of novel o-aminophenol derivatives through an efficient multi-step process, characterize them using modern spectroscopic techniques, and evaluate their antimicrobial, antioxidant, and cytotoxic activities.MethodsA series of novel derivatives of o-aminophenol have been successfully synthesized with very high efficiency through a simple six-step process using readily available chemicals and straightforward reactions. The structures of all products were accurately determined using modern spectroscopic methods such as 1D and 2D NMR, as well as IR, MS spectroscopy, and X-ray methods. The antimicrobial activities of eight o-nitrophenol derivatives were assessed against Gram (-) and Gram (+) bacteria as well as fungi. In comparison, antioxidant activities were tested for two o-nitrophenol and 11 o-aminophenol derivatives using SC50 and EC50 assays. Cytotoxicity was evaluated on KB, HepG2, A549, and MCF7 cancer cell lines.ResultsSix synthesized compounds 5b, 5c, 5g, 6b, 6c, 6g exhibited unusual doublet signals in the H8 region of the 1H NMR spectrum, attributed to atropisomer formation. Eight o-nitrophenol derivatives demonstrated weak antimicrobial activity, with MIC values ranging from 100 to 200 µg/mL. Compound 5g showed activity against all tested bacterial and fungal strains. In antioxidant testing, eight o-aminophenol derivatives 6a, 6b, 6c, 6e, 6f, 6h, 6i, and 12b displayed excellent activity, with SC50 values between 18.95 and 34.26 µg/mL, approaching ascorbic acid's SC50 value of 12.60 µg/mL. Three derivatives 6d, 6g, and 12a showed superior antioxidant activity with EC50 values between 4.00 and 11.25 µg/mL, surpassing quercetin's standard of 9.8 µg/mL. Cytotoxicity assays revealed that o-aminophenol derivatives 6b, 6c, 6f, 6i, and 12b exhibited moderate inhibitory effects on KB cell lines with IC50 values from 32 to 74.94 µg/mL. Compound 6i demonstrated moderate cytotoxic activity against HepG2, A549, and MCF7 cell lines, with IC50 values of 29.46, 71.29, and 80.02 µg/mL, respectively.ConclusionDesign, synthesis, antimicrobial activity, DPPH Radical Scavenging, Cytotoxic activity, Evaluation of H8 signal anomalies in certain compounds, and Single crystal X-ray diffraction analysis.
研究领域:
药物化学
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有机合成
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药理学
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肿瘤学
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微生物学
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天然产物化学
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分子药理学
✓ 已验证
DOI
PubMed
高影响因子
FITOTERAPIA
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2020
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IF 2.527
Xiu-Qing Song,Jia Sun,Jin-Hai Yu,Jun-Sheng Zhang,Jie Bao,Hua Zhang
Six new compounds including four prenylated indole alkaloids ( 1 – 4 ) and two lignans ( 5 – 6 ), along with eight known cometabolites ( 7 – 14 ), were isolated from the flower buds of Tussilago farfara . Structures of the new compounds were elucidated by comparison with structurally related known analogues and also by comprehensive spectroscopic analyses. Their absolute configurations were determined by a variety of means including Mosher's method, Marfey's analysis, electronic circular dichroism (ECD) exciton chirality method and ECD calculations. Our bioassays have established that compounds 1 and 2 showed potent α -glucosidase inhibitory activity with IC 50 values of 105 ± 4.7 and 35.2 ± 3.2 μM, respectively, while the known 13 and 14 exerted moderate DPPH radical scavenging activity with IC 50 values of 45.2 ± 2.9 and 29.2 ± 2.0 μM, respectively.
研究领域:
植物化学
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天然产物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
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2024
·
IF 4.3
Minmin Chen,Xiaoqun Wang,Xiawen Zhou,Baiyu Huang,Yujie Zhao,Haiying Liu,Qiuling He
Tetrastigma hemsleyanum , a traditional Chinese medicinal plant with anti-inflammatory, anti-cancer, and anti-tumor properties, faces increasing abiotic stress due to climate change, agricultural chemicals, and industrialization. This study investigated how three abiotic stress factors influence antioxidant enzyme activity, MDA levels, DPPH free radical scavenging capacity, chlorophyll, carotenoids, active compounds, and gene expression in different T. hemsleyanum strains. The comprehensive evaluation indicates that the ZJWZ strain holds potential as a preferred parental material for future resistance breeding. Furthermore, PAL gene expression was strongly positively correlated with flavonoid and phenol contents, highlighting its role in the stress response through the phenylpropanoid-flavonoid pathway. This study contributes to the standardization of the production and breeding of superior strains of T. hemsleyanum . It also lays the foundation for investigating how plants react to environmental stressors.
研究领域:
植物学
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药用植物学
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环境生物学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
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IF 3.7
Rundong Yang,Siyan Liu,Feifei Wang,Shuyi Li,Na Zhang,Zhenzhou Zhu
Laminaria polysaccharides (LP) have been shown to effectively stabilize selenium nanoparticles (SeNPs), forming LP–SeNP complexes with enhanced bioactivity. However, the correlation between their bioactivity and physicochemical properties remains inadequately explored. This study used chemical reduction with LP as stabilizer to investigate how LP-to-selenium mass ratio (LPSMR), reaction time, and temperature influence particle size, selenium content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of LP–SeNPs. The results showed that enhanced DPPH scavenging correlated with higher selenium content and smaller particle size, primarily modulated by LPSMR and temperature. Under optimal conditions (LPSMR of 1; temperature of 25°C), the resulting LP–SeNPs exhibited uniform morphology with a particle size of 81.41 nm and selenium content of 653.91 mg/g. This stability was achieved through non-covalent interactions between LP and SeNPs, providing superior light and acid resistance compared to unmodified SeNPs. Notably, LP–SeNPs showed synergistic antioxidant effects, with lower half-maximal inhibitory concentration (IC 50 ) values for scavenging DPPH, hydroxyl, and superoxide anion radicals than LP or SeNPs alone, and enhanced hypoglycemic activity. Cytotoxicity assays confirmed LP–SeNPs had reduced toxicity compared to Na 2 SeO 3 and selenopeptide. These findings provide insights into the structure-activity relationships of LP–SeNPs and support their potential application as antioxidant and hypoglycemic agents.
研究领域:
纳米技术
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药学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 10
·1897
·
IF 3.06
Andreea David,Fang Wang,Xiaoming Sun,Hongna Li,Jieru Lin,Peilei Li,Gang Deng
In the present study, the composition of essential oil isolated from the roots ofVetiveria zizanioides(L.) Nash, harvested in China, was studied, along with the bioactivities. A green novel method using an eco-friendly solvent, CO2-pressurized ethanol, or carbon dioxide expanded ethanol (CXE) was employed to isolate the essential oil from the root ofVetiveria zizanioides(L.) Nash with the purpose of replacing the traditional method and supercritical fluid extraction (SFE). After investigating the major operating factors of CXE, the optimal conditions were obtained as follows: 8.4 MPa, 50 °C, 5 mL/min ethanol, and 0.22 mole fraction of CO2, presenting an extraction oil that ranged from 5.12% to 7.42%, higher than that of hydrodistillation (HD) or indirect vapor distillation (IVD). The Gas Chromatography-Mass Spectrometry (GC-MS) analysis showed that three major components, including valerenol (18.48%), valerenal (10.21%), and β-Cadinene (6.23%), are found in CXE oil, while a total of 23 components were identified, 48 components less than using conventional hydrodistillation. Furthermore, the antimicrobial activities of root oils were evaluated by the microdilution method, which showed that CXE oil exhibited an ability against Gram-positive bacteria, especiallyStaphylococcus aureus, approximately equivalent to traditional samples. Additionally, the DPPH free radical scavenging assay demonstrated that the antioxidant abilities of root oils were sorted in the descending order: IVD > HD > CXE > SFE. In conclusion, after a comprehensive comparison with the conventional methods, the CXE-related technique might be a promising green manufacturing pattern for the production of quality vetiver oil, due to the modification of ethanol by the variable addition of non-polar compressible CO2, ultimately resulting in a prominent dissolving capability for the extraction of vetiver solutes.Keywords:vetiver essential oil;CO2expanded ethanol;hydrodistillation;chemical composition;antimicrobial activity;DPPH free radical scavenging assay
研究领域:
植物化学
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药理学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·230
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IF 4.7
Hua Li,Yurong Mao,Danni Ma,Hua Li,Ruixin Liu,Sirithon Siriamornpun
Water boiling under atmospheric pressure (CAP) and water boiling under high pressure (CHP) are two popular domestic cooking methods for Chinese porridge making. In this study, we aimed to evaluate the effects of these two methods on the phenolic acid composition, antioxidant activity, and starch digestibility of triticale porridges. The contents of total free and total bound phenolic acids in the CHP sample were 1.3 and 1.6 times higher than those in the CAP counterpart, respectively, although the DPPH and ABTS values of these two samples were comparable. CAP induced more small pieces of starch than CHP, and the gelatinization enthalpy was 19% higher in the CHP sample than that in the CAP. Both cooking methods increased the starch digestibility, while the CHP sample (58.84) showed a lower GI than the CAP (61.52). These results may promote the application of triticale in health-promoting staple foods.Keywords:phenolic acid;DPPH radical scavenging ability;ABTS radical scavenging ability;resistant starch;starch digestion;glycemic index;cereal
研究领域:
食品科学
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营养学
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化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Qin Li,Binjin Zhao,Jing Shi,Jianbo Chu,Xinyue Zhang,Qiyuan Shi
In this study, the potential of supramolecular solvent/equilibrium solution (SUPRAS/EqS) for the extraction of caffeoylquinic acids from the flowers of Artemisia anomala (AAF) coupled with re-extraction was evaluated. Extraction and re-extraction parameters were optimized based on the yield of 3-chlorogenic acid, isochlorogenic acid A-C. The DPPH radical scavenging and xanthine oxidase inhibitory activity of the final extracts were also evaluated. SUPRAS/EqS, made up by n -octanoic acid, ethanol, 5% acetic acid aqueous solution, offered advantages in terms of rapidity (extraction for 1 min) and simplicity (the process involved vortex-shaken and centrifugation at room temperature), thus avoiding cost processes based on high pressure or temperature. Multi-stage countercurrent extraction (MSCE) procedure was successfully applied to reduce the consumption of extraction solvent (24-fold volume of raw materials in total). SUPRAS/EqS-MSCE extracts were evaporated to recycle ethanol and then re-extracted by water for 3 times (with a total consumption of 9-fold volume of material weight). The contents of 3-chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C in the final extract were 3.61 ± 0.01, 2.32 ± 0.01, 26.42 ± 0.05, 5.38 ± 0.01 mg/g dry weight of AAF with a recovery rate of 95.0%, 92.7%, 90.6% and 94.5%, respectively. Furthermore, the final extract of AAF exhibited moderate DPPH radical scavenging activity (IC 50 value was 839. 46 ± 1.02 μg/mL, vitamin C’s was 220.29 ± 1.02 μg/mL) and weak xanthine oxidase inhibitory activity (IC 50 value was 4518.56 ± 1.03 μg/mL, quercetin’s was 103.04 ± 1.04 μg/mL).
研究领域:
植物化学
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提取技术
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药理学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Shuqi Guo,Xueze Zhang,Shangxuan Li,Huifang Wang,Xianghua Gao,Baolong Niu,Wenfeng Li
Quercetin (Que) is a naturally occurring bioactive compound with various potential health benefits, but its low stability and low bioaccessibility limit its application in functional foods. Therefore, there is an urgent need to prepare a novel composite hydrogel bead with efficient and controlled release. Specifically, porous starch (PS) was first prepared by enzymatic hydrolysis to increase the specific surface area and thus enhance the adsorption rate of Que. Scanning electron microscopy confirmed the formation of the PS and the adsorption of Que. The maximum swelling properties of carboxymethylcellulose-porous starch composite hydrogel beads (CQPZ) were reduced by adding different amounts of zinc oxide to achieve slow release. The results showed that the higher the amount of zinc oxide, the stronger the slow-release performance, while the lower the drug loading capacity. To obtain composite hydrogel beads with both low swelling and high drug-carrying properties, bilayer gel beads (CQPZ/CS) were prepared by electrostatic self-assembly of carboxymethyl cellulose and chitosan. The experimental results showed that the drug loading was significantly increased by nearly 2-fold while enhancing the slow-release performance. In addition, the CQPZ/CS composite hydrogel beads enhanced the stability of Que under light and heat conditions, and the DPPH radical scavenging rate after UV irradiation was still greater than 75%, which was much higher than that of free quercetin (31.08%). In conclusion, the prepared hydrogel beads are a promising delivery system for bioactive compounds.
研究领域:
食品科学
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药剂学
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材料科学
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生物技术
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）